User manual

56-3124-15 Edition AF 2003-08 9
BufferPrep mode
If a method compilation error appears when trying to run a BufferPrep mode method,
the method does not start but the BufferPrep recipe is still downloaded to the Pump
instrument module. An error will then occur as soon as another BufferPrep recipe is
downloaded, e.g. if you try to run a BufferPrep method again. This error will also occur
when a BufferPrep recipe is selected manually before a method running in BufferPrep
mode is started and when running a method which includes BufferPrep.
Action: To “remove” the downloaded BufferPrep recipe from the Pump module, execute
any instruction, e.g. a Flow instruction, and then press the End button. (The dummy
instruction must be executed for the End button to be enabled.)
This action applies to all situations where you want to abort BufferPrep mode.
PumpWash
If you press the Continue button during PumpWash execution the Pump module may be
set in End mode although the method continues to execute. This affects the whole
method execution and will most probably give incorrect results.
Action: Never press Continue during PumpWash execution.
Module calibrations
Calibrations made directly on ÄKTAdesign system modules are logged only in the System
Control logbook and not in the Calibration page of Documentation or in the Audit Trail.
This means that Calibration page and Audit Trail information might not be up-to-date.
Action: Always calibrate ÄKTAdesign system modules by using UNICORN calibration
functions in System Control.
Alarm during PumpWash or SystemWash execution
If an ALARM event occurs during PumpWash, the system is Paused as intended but is
Continued when PumpWash has ended. (The same applies to the SystemWash
instruction.) Normally, the system will remain in Pause after an ALARM.
Action: No action possible.
Outlet valve positions
The following sequence will cause the outlet valve positions to be wrong when used with
10 and 100 system strategies in combination with a Frac-900:
Insert a flowrate and fractionation command
Switch Outlet Valve to F1
Switch back Outlet Valve to F2
This will cause the Outlet Valve to be switched to F2, but it will immediately switch back
to F1. This is not noted in the logbook. Any other position than F2 for the Outlet Valve
works well. The problem occurs both if the sequence is used manually or in a method.
Action: Give the F2 command again or choose another outlet position.