User guide

6 Dako Autostainer | User Guide
Adding Slides................................................................................................................. 39
Deleting a Slide.............................................................................................................. 40
The Slide Information Button............................................................................................. 40
Entering Slide Information Through the SLIDE INFORMATION Button ........................ 41
Deleting Slide ID Information through the SLIDE INFORMATION Button..................... 42
Deleting a Slide Identification ........................................................................................ 42
Deleting a Case # .......................................................................................................... 43
Deleting a Block ID ........................................................................................................ 43
Deleting a Specific Slide ................................................................................................ 44
Slide Rearrangement ........................................................................................................ 44
Rearranging slide positions on the Programming Grid .................................................. 44
Section 9 | Designing a Protocol ....................................................................................... 45
Protocol Elements ............................................................................................................. 46
Rinse Buffer ................................................................................................................... 46
Rinse Water ................................................................................................................... 46
Standard Rinse Replacement........................................................................................ 46
Substrate-Batch ............................................................................................................. 47
Switch (*Swtch).............................................................................................................. 47
Creating and Editing a Protocol Template......................................................................... 47
Saving a Protocol Template........................................................................................... 48
Deleting a Protocol Template ........................................................................................ 49
Using a New Protocol Template Without Saving It ........................................................ 50
Using a Saved Protocol Template ................................................................................. 50
Using a Saved Protocol Template for a Specific Auto Program .................................... 50
Selecting and Editing the Reagent Dispense Volume....................................................... 51
Selecting and Editing the Reagent Dispense Volume for All Steps in a Protocol.......... 51
Selecting and Editing the Reagent Dispense Volume for a Specific Step in a Protocol 52
Section 10 | Programming Slides ...................................................................................... 53
Defining Slide Specific Reagents ...................................................................................... 53
Defining Detection Reagents (Secondary, Tertiary, Substrate, etc.) ............................. 53
Defining Primary Antibodies .......................................................................................... 54
Assigning Positive and Negative Control Reagents ...................................................... 55
Defining Specific Rinses ................................................................................................ 55
Auto Programming ............................................................................................................ 56
Creating and Saving an Auto Program .......................................................................... 56
Deleting an Auto Programming Item.............................................................................. 57
Using an Auto Programming Item.................................................................................. 58
Getting Help with Auto Programming ............................................................................ 58
Copy/Paste........................................................................................................................ 58
Copy/Paste Individual Reagents.................................................................................... 58
Copy/Paste Entire Rows................................................................................................ 59
Editing a Protocol Step...................................................................................................... 59
Changing a reagent for a step: ...................................................................................... 59
Replacing a reagent with skip step (no reagent added to slide): ................................... 60
Editing a reagent in a staining step for one run only:..................................................... 60
Printing Options................................................................................................................. 61
Slide Labels ................................................................................................................... 61
Reagent Labels.............................................................................................................. 61
Printing reagent labels not from a program. .................................................................. 62
The Main Grid Report .................................................................................................... 62
Immunohistochemical Report (IHC Report)................................................................... 63
Run Log Printout............................................................................................................ 63
Viewing Programmed Slides.......................................................................................... 64
Assigning Reagent Dispense Locations............................................................................ 65
Assigning Reagent Dispense Volumes ............................................................................. 66