User guide

76 Dako Autostainer | User Guide
Compatibility Check
A compatibility check has been built into the Dako Autostainer software to minimize programming
errors resulting from assigning incompatible reagents (such as alkaline phosphatase-labeled
streptavidin and DAB substrate) to a slide protocol. This feature has two check levels. The first check
is the species reactivity compatibility of the primary antibody and the secondary reagent used. The
second addresses the compatibility rules dictated by the enzyme used in the detection system. The
following tables summarize the compatibility rules and codes assigned to each reagent type.
A. Species Reactivity
Reagent Type
(Protocol Element)
Description Compatibility
Code
Primary Antibody All monoclonal primary antibodies raised in
mouse (e.g., mouse anti-human)
A
Primary Antibody All polyclonal primary antibodies raised in rabbit
(e.g., rabbit anti-human)
B
Secondary Reagent All secondary reagents (antibodies) compatible
with monoclonal antibodies raised in mouse
(e.g., biotinylated anti-mouse IgG)
A
Secondary Reagent All secondary reagents (antibodies) reacting
with polyclonal antibodies raised in rabbit (e.g.,
biotinylated anti-rabbit IgG)
B
B. Enzyme System Compatibility
Reagent Type
(Protocol Element)
Description Compatibility
Code
Endogenous Enzyme Block, HRP-compatible X
Endogenous Enzyme Block, AP-compatible Y
Tertiary Reagent HRP-labeled streptavidin X
Tertiary Reagent AP-labeled streptavidin Y
Substrate HRP-compatible substrates (i.e. DAB, AEC) X
Substrate AP-compatible substrates (i.e. Fast Red, New
Fuchsin)
Y