Specifications

PN 718113AF
2
Laser Induced Fluorescence Detection
System Overview — LIF System
System Overview — LIF System
The LIF system consists of an instrument, along with the LIF Detector module, the LIF Interconnect
Module, and a Laser Module. Refer to Figure 1 for more information. A regular instrument capillary
cartridge with a LIF Detector plug installed is required for use with this system. The system requires
the instrument software, version 2.0 or later.
The LIF Detector
The Laser Induced Fluorescence (LIF) Detector is available as an optional accessory. The LIF detector
uses a laser light source. A 488 nm argon-ion laser is available from Beckman Coulter. Other lasers
can be adapted. Refer to the section titled Using Other Lasers with the LIF Detector for more
information. A fiber cable transmits excitation light from the laser to the capillary in the cartridge.
Substances in the capillary which fluoresce at the laser wavelength are detected. The LIF Detector
measures and records this fluorescence, which appears as a peak in the software.
The initial installation of the LIF Detector must be performed by a Beckman Coulter Field Service
Engineer. The instrument can easily be converted between LIF and UV/PDA modes as detector
components are modular.
The LIF Detector can be used in the indirect mode. In this mode, a fluorescent buffer system is used
to detect non-fluorescing components.
Component Description
The laser is used as the excitation source. The Interconnect Module is used to connect the laser to
the instrument. A fiber optic cable and probe directs the laser light to the capillary. The LIF
cartridge plug focuses the emitted light into the LIF Detector Module. The LIF detector collects the
fluorescence emissions and converts it into an output signal. Filters are installed in the detector
filter housing to correspond with the lasers and chemistry being used with the system.
Power requirements 100/120V, 12A, 50/60 Hz
or 220/240V, 6A, 50/60 Hz
Ambient Temperature Operating Range 15 to 40°C (39 to 104°F)
Recommended Best Performance Range 15 to 30°C (59 to 86°F)
Relative Humidity <95% non-condensing @ 35°C (121°F)
a. These specifications are for a 75 mm I.D. capillary.
Table 1 LIF Detection Specifications (Continued)
Specification Value