Specifications

PN 718113AF
11
Laser Induced Fluorescence Detection
Installation
Installing the LIF Detector Module
Prior to installing the LIF Detector, install the filter(s) and filter housing.
Installing Filters in the Filter Housing
The filter housing, which is inserted in the right side of the LIF Detector, has two filter holders, one
for each detector channel. Refer to Figure 6 for a detailed diagram of the Filter Housing. One or
more filters can be used in each filter holder, depending on the application and the width of the
filter. During typical operation two filters are used: one laser filter and one emission filter. The
laser filter prevents scattered laser light from passing into the detector. The emission filter is a
band pass filter that allows only the fluorescence signal at a selected wavelength to pass into the
detector.
Beckman Coulter supplies the following filters:
• 520 nm, 560 nm and 675 nm emission
• 488 nm laser filter (notch filter)
Depending on the application and laser, you may use any standard 0.5 inch diameter optical filter
in the range of 350 to 750 nm.
To install filter(s) into the filter holder (refer to Figure 6):
1 Hold the filter housing in the same orientation as shown in the drawing.
2 Remove the beam blocker if present.
3 Hold the filter with the arrow on the outside edge of the filter pointing up.
(If the filter does not have an arrow on its edge, refer to the note below). Be sure to handle filters
by the edges.
4 Carefully place the filter into the filter holder.
5 When all filters are in the filter holder, insert the spring and install the filter holder in the
appropriate location (channel 1 or 2) in the filter housing.