Specifications

PN 718113AF
23
Laser Induced Fluorescence Detection
Operation
status on the Direct Control and Status screen reflects the position of this on/off switch and not the
state of the laser.
Time Program
When programming a method with LIF Detection, select Method > Instrument Setup.
The instrument setup window will appear. See Figure 18.
Figure 18 LIF Detector Initial Conditions Screen
NOTE Excitation and Emission Wavelength settings are for indication purposes only. The excitation and
emission wavelengths are determined by the laser and filter combination installed in the LIF detector.
Dynamic range: A Dynamic range of 1.0 through 1000 RFU can be selected.
Dynamic Range
In the IEEE Standard Dictionary of Electrical and Electronics Terms, the dynamic range is defined as
“The range of output levels that can be read, from saturation level to the level of minimum
discernible output signal.” For the LIF detector, the upper limit of the dynamic range corresponds
to the maximum output signal from the detector (the highest RFU value detectable). If a dynamic
range is selected which is lower than the largest peak, peak tops will be truncated and flattened
(see Figure 19).