Specifications

PN 718113AF

Laser Induced Fluorescence Detection

Operation

Figure 19 Electropherograms (A, B) with different Dynamic Ranges

For optimal results, select a dynamic range slightly larger than the tallest peak in the

electropherogram.

NOTE

The higher the dynamic range, the higher the relative noise level in the electropherogram. This effect

is not significant at dynamic range settings of ≤ 100, but is clearly visible at a dynamic range setting of

1000. For optimal sensitivity, use a dynamic range setting of ≤ 100, even if this would involve dilution of

the sample prior to injection.

Calibration Correction Factor

Unlike absorbance detectors, there is no absolute reference point for fluorescence detectors;

measurements are reported relative to a defined “standard” in relative fluorescence units (RFU).

This measurement is dependent on a number of parameters including but not limited to:

• Capillary internal diameter

• Concentration of the fluorescent species

A. Electropherogram with Dynamic Range set to 100.

B. Same Electropherogram with Dynamic Range set to 10. Note that the heights of all

peaks have exceeded the dynamic range setting and were truncated.