User manual

66 1200 Series Variable Wavelength Detector User Manual
5 How to optimize the detector
Optimizing the Detector Performance
The detector has a variety of parameters that can be used to optimize
performance.
The information below will guide you on how to get the best detector
performance. Follow these rules as a start for new applications. It gives a
rule-of-thumb for optimizing the detector parameters.
Match the Flow Cell to the Column
Figure 26 recommends the flow cell that matches the column used. If more
than one selection is appropriate, use the larger flow cell to get the best
detection limit. Use the smaller flow cell for best peak resolution.
Figure 26 Choosing a Flow Cell
Typical peak
width
Column length Recommended flow cell
Internal column diameter
Typical flow rate 0.05 – 0.2 ml/min
1.0 mm 2.1 mm 3.0 mm 4.6 mm
0.2 – 0.4 ml/min 0.4 – 0.8 ml/min 1 – 2 ml/min
<= 5 cm
10 cm
20 cm
>= 40 cm
0.025 min
0.05 min
0.1 min
0.2 min
Micro
flow cell
Standard
flow cell
Semi-micro
flow cell