User manual

1200 Series Variable Wavelength Detector User Manual 69
How to optimize the detector 5
Set the Detector Parameters
1 Set peakwidth as close as possible to the width (at half height) of a narrow
peak of interest.
2 Choose the sample wavelength.
• at a longer wavelength than the cut-off wavelength of the mobile phase,
• at a wavelength where the analytes have strong absorptivity if you want
to get the lowest possible detection limit,
• at a wavelength with moderate absorptivity if you work with high
concentrations, and
• preferably where the spectrum is flat for better linearity.
3 Consider to use time-programming to further optimization.
Table 12 Peakwidth Settings
Peakwidth at half height Rise time [10.. 90%] Data rate Module
< 0.00125 minutes < 0.031 seconds 54.96 Hz G1314C
0.00125 minutes 0.031 seconds 27.48 Hz G1314C
0.0025 minutes 0.062 seconds 13.74 Hz G1314C
0.005 minutes 0.125 seconds 13.74 Hz G1314B / G1314C
0.01 minutes 0.25 seconds 13.74 Hz G1314B / G1314C
0.025 minutes 0.50 seconds 13.74 Hz G1314B / G1314C
0.05 minutes 1 second 6.87 Hz G1314B / G1314C
0.1 minutes 2 seconds 3.43 Hz G1314B / G1314C
0.2 minutes 4 seconds 1.72 Hz G1314B / G1314C
0.4 minutes 8 seconds 0.86 Hz G1314B / G1314C
NOTE
The G1314C VWD-SL can be operated with a G1323B just in standard mode as G1314B - no
higher data rate selection is available.