BIOHAZARD CABINET MANUAL Model 10448 - EBC 47, 4-Foot Type A/B3 Model 10276, 6-Foot Type A Model 10451, 6-Foot Type B3 Operation Manual A Fedders Engineered Products Company Biohazard Cabinet Manual NSF Std 49 (92) 011130
CAUTIONS AND WARNINGS READ AND SAVE THESE INSTRUCTIONS WARNING! TO REDUCE THE RISK OF FIRE, ELECTRICAL SHOCK, OR INJURY TO PERSONS, OBSERVE THE FOLLOWING: A. Read this manual completely before installing or operating the ENVIRCO Biohazard Cabinet. B. Use this unit only in the manner intended by the manufacturer. If you have any questions, contact the manufacturer: ENVIRCO 101 McNeill Road Sanford, NC 27330 Tel: (919) 775-2201 Tel: (800) 884-0002 Fax: (800) 458-2379 Email: info@envirco.
E. Caution: Do not, under any circumstances, cut or remove the grounding prong from the plug. 5-20 P Plug 5-20 R Receptacle 5-30 P Plug 5-30 R Receptacle 10276, 6-Ft Type A 10451, 6-Ft Type B3 10448 - EBC 47, 4-Ft Type A/B3 Figure 1: Plug and Receptacle Requirements F. Unplug the power cord and decontaminate before servicing the cabinet or changing the filters. Do not use extension cords. If the power cord or plug becomes frayed, cracked, broken or otherwise damaged, replace it immediately. G.
TABLE OF CONTENTS CAUTIONS AND WARNINGS...................................................................................................2 TABLE OF CONTENTS .............................................................................................................4 I. INTRODUCTION ............................................................................................................5 II. DEFINITIONS...................................................................................................
I. INTRODUCTION Investigators and technicians concerned with microbiological safety have for many years utilized specialized containment enclosures to limit their exposure to harmful pathogens. In addition, it is recognized that exacting research, as well as routine pathology, is often compromised by environmental contamination. The ENVIRCO Class II Biohazard Cabinet is designed to limit the contamination exposure of both the worker and the work when handling biohazard material.
Class II, Type A/B3 Cabinets: 1. Meets the requirements of Class II, Type A cabinets when the HEPA filtered exhaust air is recirculated back to the laboratory. 2. Meets the requirements of Class II, Type B3 cabinets when the exhaust air is vented to the outside atmosphere after HEPA filtration. 3. Confirms to the requirements for either the Type A or Type B3 cabinet outlined above. III.
IV. FEATURES 1. The hinged viewscreen is constructed with safety glass and has a sevendegree angle for comfortable viewing. The hinge enables easy loading and unloading of work and allows maximum accessibility when cleaning the interior work area. 2. The all stainless steel work area has a lift-out work tray and support for easy cleaning. 3. The self-contained drain pan with external drain valve simplifies flushing and cleanup of major spills. 4.
V. CABINET DIMENSIONS Model A B C D E F 10448 – EBC 47, 4-Ft Type A/B3 47.0” 32.5” 62.0” 58.0” 4.0” 28.5” 10276, 6-Ft Type A 10451, 6-Ft Type B3 70.0” 32.5” 62.0” 58.0” 4.0” 28.
VI. SPECIFICATIONS Electrical: 10448 - EBC 47, 4-Ft Type A/B3: 115v, 60 Hz, 20 amp 10276, 6-Ft Type A: 115v, 60 Hz, 30 amp 10451, 6-Ft Type B3: 115v, 60 Hz, 30 amp 10448 - EBC 47, 4-Ft Type A/B3: Single power cord with a NEC 5-20p cap plug 10276, 6-Ft Type A: Single power cord with a NEC 5-30p cap plug 10451, 6-Ft Type B3: Single power cord with a NEC 5-30p cap plug 10448 - EBC 47, 4-Ft Type A/B3: One (1) 3/4 h.p.
Airflow 10448 - EBC 47, 4-Ft Type A/B3: Direct Inflow Method -Velocity -Airflow Type A/B3 100 – 110 fpm 253 – 278 cfm Calculated Face Velocity Exhaust Volume Exhaust Filter Area Front Access Area 115 – 125 fpm 290 – 316 cfm 1.07 sq. ft. 2.53 sq.
NOTES: 1. In performing the velocity profile test, the front row of velocity readings (behind the window) must be taken with the thermoanemometer probe positioned perpendicular to the window with the probe head facing the window. 2. Velocity readings for the middle row(s) and rear row must be taken with the probe positioned parallel to the window. 3.
VII. INSTALLATION Cabinet Location The cabinet should be placed in an area of the laboratory free from strong air currents, heavy traffic and particulate microbial or chemical contamination. This will reduce any disruption of the airflow patterns at the face opening and will prolong the life of the HEPA filters. The ceiling height above the cabinet should allow four (4) inches of clearance above the exhaust area to allow free air passage. Installation and Leveling Caution: Do not remove the front panel.
Service Connection Make sure that a 20 amp, 115 V outlet with the proper receptacle is available for the 10448 - EBC 47, 4-Ft Type A/B3. Both the 10276, 6-Ft Type A and the 10451, 6-Ft Type B3 units require a 30 amp, 115 V outlet with the proper receptacle. Connect the air, gas or vacuum service line to the external connection point located on the top of the cabinet. The connections should be field tested for leak tightness.
External Exhaust Connection (Optional) Under normal conditions, the Class II Type A Cabinet does not require external exhaust from the laboratory. However, when used in the Type B3 configuration, the cabinet must be connected to an external laboratory exhaust system with the use of a thimble connection. This ensures that the exhaust air balance of the Biohazard Cabinet is not disturbed by fluctuation in the external exhaust system.
10” diameter centered on thimble 20.87” 26.50” 5.35” 3.50” 20.25” 22.25” 2.90” 2.65” 20.87” 26.56” 14.25” 6.
VIII. TESTING AND CERTIFICATION The ENVIRCO Biohazard Cabinet has been fully tested by the manufacturer prior to shipment, however, shipping and handling during installation may result in leaks in the HEPA filter or other cabinet seals. The cabinet should be adjusted for proper airflow balance after it has been installed in the final location. Recommended Tests The following tests are recommended at initial installation: 1. Aerosol Leak Test of the recirculating air HEPA filters. 2.
Re-certification It is recommended that the ENVIRCO Biohazard Cabinet be re-certified every six (6) months and the HEPA filters are replaced if necessary. An Authorized Service Contractor can provide this service upon request and should be contacted directly.
Testing 1. Calibrate the photometer with the aerosol generator so that the upstream concentration is 100 percent. The upstream concentration for all HEPA filters may be sampled by removing the pipe plug from the penetration provided on the top front of the cabinet and inserting the photometer probe in the penetration fitting. Set the photometer to ZERO and verify the 100 percent upstream and zero downstream readings are achieved. Set the range switch to read a minimum of 0.01 percent. 2.
Drawing Number PD – 10448-EBC 47 8 FT POWER CORD WITH NEMA TYPE 5-20P CAP PLUG LAB SERVICE WIRING DIAGRAM SIDE SECTION FRONT ELEVATION (Access Panels Removed for Clarity) IX.
Drawing Number PD – 10276 / 10451 WIRING DIAGRAM SIDE SECTION FRONT ELEVATION (Access Panels Removed for Clarity) X.
XI. FILTER REPAIRS Caution: If the cabinet has been used with infectious agents, decontamination is necessary before removing access panels. Shipping movement and handling may cause filter leaks. These leaks can normally be repaired as follows: 1. Caulking with silicone RTV can usually repair small leaks in the filter media or at an adhesive joint from the downstream side of the HEPA filter. 2. Leaks at gasket seal may also be stopped with the RTV sealant on the downstream side.
AIRFLOW ADJUSTMENT Airflow adjustments can be made with either the solid state speed control inside the electrical control panel and/or with the exhaust damper. Access to the damper adjusting rod is gained through the plugged access hole in the front access panel of the cabinet. The recirculating supply airflow and the exhaust airflow velocities must be set per values on page 10 of this manual.
Rear of work deck 5.88” 5.88” 6.00” Probe set at the bottom edge of the window 7.25” 6.00” (Typical) Figure 9: Downflow Velocity Grid, Models 10276, 6-Ft Type A and 10451, 6-Ft Type B3 Inflow Airflow Measurement (Type A & Type B3 Units) Direct Inflow Method The inflow airflow is measured by placing the 8” x 24” hood directly in front of the window and blocking off the entire remaining opening.
3.58” 4.00” 4.00” 3.08” Flange of Exhaust Collar 4.00” Flange of Exhaust Collar 3.08” Exhaust Velocity Grid Exhaust Velocity Grid 10448 EBC 47, 4-Ft Type A/B3 10276, 6-Ft Type A 10451, 6-Ft Type B3 Figure 10: Exhaust Velocity Grid XIII. POST-INSTALLATION TESTING Visual Containment Test After the cabinet has been balanced for proper air velocity readings, it should be checked with a simple visual smoke test. 1.
Electrical Tests Electrical grounding tests should be made to assure that all exposed metal surfaces of the cabinet are grounded. Equipment 0-1 ohm full scale ohmmeter Testing 1. Disconnect the cabinet from the power source. 2. Connect one lead of the ohmmeter to the ground pin on the power cord plug. 3. Touch the other lead to the ground pin on the convenience outlet, stainless steel work deck, viewscreen frame, and several of the exposed screw fasteners on the front of the cabinet. 4. XIV.
Surface Maintenance Painted surfaces and the outside of the safety viewscreen may be cleaned with soap and water. A light abrasive soap can be used on the stainless steel areas to help remove scratches and stains. Rub in the direction of the grain only. Use Of Ancillary Equipment Do not use equipment requiring more than three (3) amperes maximum. Bunsen Burner Caution: Bunsen Burners should never be operated unless the cabinet blowers are turned “ON.
Working in the Biohazard Cabinet Caution: Because of the highly specialized nature of the ENVIRCO Biohazard Cabinet, it is recommended that an Authorized Service Contractor perform all service and maintenance. A maintenance and service decontamination procedure is available upon request. 1. The cabinet is ready to use only after an Authorized ENVIRCO Service Contractor has completed on-site testing and certification procedures. 2.
XV. CABINET PRESSURE TESTING The ENVIRCO Biohazard Cabinet is designed so that there are no contaminated plenums under positive pressure when the cabinet is operating. The entire outer shell of the cabinet and the work area are at negative pressure with respect to the laboratory. Therefore, any leakage at the cabinet penetrations or at the access panels is "inward" and thus not a contamination hazard.
XVI. LABELING USED ON THE CABINET Review all labeling on the ENVIRCO Biohazard Cabinet as it provides both the installer and the worker with important information regarding cabinet function and safety. IMPORTANT WARNING Do Not Plug in Cabinet. Service Connection(s) Do Not Use Cabinet Until Certification Has Been Completed.
WIRING DIAGRAMS 6 5 4 3 2 1 1 2 3 BLK CONN 'C' WHT WHT GRN BLK BLK CONN 'G' GRN NEMA 5 20P 3 AMPS MAX WHT BRN M1 BRN 3 2 1 BLK WHT BLK 370V 15 MFD BLK WHT GRN BRN BRN XVII.
6 5 4 3 2 1 6 5 4 3 2 1 1 2 3 GRN 'C' BLK NEMA 5 20P WHT 'E1' WHT GRN BLK WHT 3 AMPS MAX BLU BRN 'E2' BRN M1 BLU WHT 'B' 3 2 1 BLK WHT BLK BLK WHT WHT BRN BRN BRN GRN BRN BRN BRN M2 10 9 8 7 6 5 4 3 2 1 370V 15 MFD BRN BRN BRN BRN SSSC 2 1 WHT WHT SSSC 2 1 WHT WHT BLK BLK SENSOR BLU BLU BLK BLK BUZZER BLK 1 WHT 2 BLK BLK BLU WHT CONN 'H' BLK BLOWER 15 AMPS RECP 3 AMPS LIGHTS 3 AMPS BLK BLK 1 WHT 2 WHT WHT RED 6 RED BLU 5 BLU CONN 'K' Figure
XVIII. HEPA FILTER REPLACEMENT The HEPA filters require replacement when the air resistance is such that the specified air velocities cannot be achieved. Replacement should also be made when the HEPA filter is damaged beyond repair or if five percent or more of the filter surface has been patched. Only an Authorized Service Contractor should replace the HEPA filters. Filter Removal Caution: Do not remove the front panel until the cabinet has been decontaminated.
XIX. REPLACEMENT AND OPTIONAL PARTS DESCRIPTION Assembly, Motor/Blower Motor, 3/4 H.P. O.D.P., 1625 rpm Motor, 1/2 H.P. O.D.P.
XX. BIBLIOGRAPHY 1. Effective Use of the Laminar Flow Biological Safety Cabinet. U.S. Department of Health, Education and Welfare, Office of Biohazard and Environmental Control, Bethesda, MD. 2. Federal Standard No. 209B, U.S. Government Printing Office, 1973, Superseding Federal Standard No. 209A, 1966. 3. National Cancer Institute Safety Standards for Research Involving Chemical Carcinogens, DHEW Publication No.(NIH) 76-900, 1975. 4. NSF International Standard No.
XXI. GLOSSARY Aerosol: A colloid of liquid or solid particles suspended in a gas, usually air. Ambient: Surrounding on all sides. Biohazard: A contraction of the words biological and hazard. Infectious agents presenting a risk or potential risk to the well being of man, or other animals, either directly through infection of indirectly through disruption of the environment. Carcinogen: A substance or agent producing or inciting cancer.
XXII. RISK LEVELS, CLASSIFICATION Low Risk The risk level of the agents and/or the operation has minimal effect on personnel, animals, or plants under ordinary conditions. This classification is restricted to all etiological agents designated Class I by the U.S. Department of HEW, Center for Disease Control. Moderate Risk The risk level of the agents and/or the operations requires special conditions for control or containment because of: 1. Known pathogenicity to personnel, animals, or plants. 2.
XXIII. MANUFACTURER'S LIMITED WARRANTY The Biohazard Cabinet is manufactured by ENVIRCO, of Albuquerque, New Mexico and is warranted to be free from defects in material and workmanship under normal use. This warranty is subject to the following qualifications, conditions and limitations set forth below to provide information concerning the duration and extent of this warranty, the procedure to be taken to obtain performance, and other information concerning the warranty policy.
