User guide

A custom method is built one step at a time by entering commands. Commands

include the following:

A) Inject

This command adds reagents. The inject range is 0.1-15mls in 0.1ml

increments.

To inject a reagent:

(1) Choose the appropriate reagent in the reagent drop down box.

(2) Enter the volume required via the keypad.

(3) Press Inject.

(4) To inject secondary reagents, repeat this process at the proper time in the

custom method.

Note: Purging the last reagent and prepping for the new reagent are

automatically taken into account by the ahb. program, and need not be

addressed in custom method development.

B) Heat To

This command determines the Set Point temperature, not sample temperature.

Range is 10°-125° C increments of 0.1°. For establishing accurate sample

temp, see section 5.3. Variable A or Variable B can be used in this field as

well. See section 5.2 for additional details. “A” is 95.0 sample temperature

without a reflux cap and “B” is 95.0 with a reflux cap. These are defaults.

Insure that they have not been modified in the maintenance mode.

C) Wait For

This command determines the amount of time the samples will be heated

during a step. Range is 1-999 minutes. The Wait For time does not start until

the Graphite temp reaches within 5 degrees of the Set Point temp. An

automatic command is sent for the elevator to lower the samples down into

the block, prior to the countdown.

D) Cool For

This command sets the amount of time the samples will be cooled. Range is

0.1-999 minutes. The speed of the fan automatically increases to it’s

maximum for efficient sample cooling.

Technical Note

When creating a custom method, samples must be cooled for a minimum of 20

minutes between heating and adding a reagent. Based on our tests, we

recommend you add 1-2 ml of DI Water prior to reagent additions. This helps

cool samples even further. HYDROGEN PEROXIDE can cause violent

reactions with some samples. A minimum of 35 minutes must be entered as a

cooling time, and you must add 2ml DI Water prior to the injection of H2O2.

7.7.1 Example of Custom Method Development