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Copyright© 2013 Eppendorf AG, Hamburg. No part of this publication may be reproduced without the prior permission of the copyright owner. Trademarks Eppendorf® and the Eppendorf logo are registered trademarks of Eppendorf AG. Microtainer® is a registered trademark of Becton Dickinson, Franklin Lakes, NJ, USA. Trademarks are not marked in all cases with ™ or ® in this manual. 5401 900.
Table of contents Centrifuge 5418 / 5418 R English (EN) Table of contents p. Tab. Operating Centrifuge on see Fig. p. manual 5418 / 5418 R English Operating (EN) manual 1 Operating instructions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5 1.1 Using this manual . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5 1.2 Danger symbols and danger levels . . .
Table of contents Centrifuge 5418 / 5418 R English (EN) 5.3 5.4 5.5 5.6 Cooling (only 5418 R) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22 5.3.1 Temperature adjustment . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22 5.3.2 Temperature display . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22 5.3.3 Temperature monitoring . . .
Operating instructions Centrifuge 5418 / 5418 R English (EN) 1 Operating instructions 1.1 Using this manual Read this operating manual completely before using the device for the first time. Please also note the operating instructions for the accessories, if applicable. This operating manual is part of the product. Thus, it must always be easily accessible. Enclose this operating manual when transferring the device to third parties. If this manual is lost, please request another one.
Operating instructions Centrifuge 5418 / 5418 R English (EN) 1.
Product description Centrifuge 5418 / 5418 R English (EN) 2 Product description 2.1 Main illustration Abb. 2-1: Front and rear view of the Centrifuge 5418 1 2 1 2 3 4 6 7 8 9 5 Fig. 2-1: Front and rear view of the Centrifuge 5418 Abb. 2-2: Front and rear view of the Centrifuge 5418 R 1 2 1 2 5 3 4 6 9 10 10 7 8 Fig.
Product description Centrifuge 5418 / 5418 R English (EN) 2.2 Delivery package 2.2.1 Centrifuge 5418 Quantity Order no. Order no. (International) (North America) 1 - - 1 or 5425 351.003 5425 353.006 022668188 022668226 1 5416 301.001 022634305 1 - - 1 1 5401 900.022 5401 900.030 5401900022 5401900030 2.2.2 Description Centrifuge 5418 See chapter Ordering Information for corresponding device version, equipment and order number Fuse 2.
Product description Centrifuge 5418 / 5418 R English (EN) 2.3 Features The compact and easy-to-use Centrifuge 5418 / 5418 R has a capacity of 18 x 2 ml and reaches a maximum of 16,873 x g / 14,000 rpm. The microcentrifuge is equipped with an aerosol-tight standard rotor for centrifugation of the following tubes: • Micro test tubes (0.2 to 2.0 ml) • Microtainers (0.6 ml) • Spin columns (1.5/2.
Product description Centrifuge 5418 / 5418 R English (EN) 2.4.1 rcf display and calculation Use the rpm/rcf key to switch the display of centrifugation speed between rcf and rpm. Only 5418 R: For speeds ≤ 800 rpm only the lowest adjustable g-force (100 x g) is displayed upon switching. The exact g-force (rcf) can be determined using the formula given below. Ensure that the g-force (rcf) displayed upon switching is standardized to suit the rotor without an adapter.
Safety Centrifuge 5418 / 5418 R English (EN) 3 Safety 3.1 Intended use The Centrifuge 5418 / 5418 R is intended exclusively for indoor use and for separating aqueous solutions and suspensions of various densities in approved test tubes. 3.2 User profile This device may only be operated by trained specialist staff. They must have carefully read the operating manual and be familiar with the function of the device. 3.3 Application limits 3.3.
Safety Centrifuge 5418 / 5418 R English (EN) Accessories Maximum service from the first commissioning onward Rotor lid 3 years Requirement: The "click" is still audible when tightening the rotor lid screw. Plastic adapters 1 year For the rotor described there is no limit on the service life as long as the following prerequisites are met: • Proper use, • Recommended maintenance • Undamaged condition The date of manufacture is stamped on the rotors in the format 03/10 (= March 2010).
Safety Centrifuge 5418 / 5418 R English (EN) WARNING! Lethal voltages inside the device. Ensure that the housing is always closed and undamaged so that no parts inside the device can be contacted by accident. Do not remove the housing of the device. Do not allow any liquids to penetrate the inside of the housing. Do not allow the device to be opened by anyone except service personnel who have been specifically authorized by Eppendorf.
Safety Centrifuge 5418 / 5418 R English (EN) NOTICE! Damage to device due to spilled liquids. 1. Switch the device off. 2. Disconnect the device from the power supply. 3. Carefully clean the device and the accessories in accordance with the cleaning and disinfection instructions in the operating manual. 4. If a different cleaning and disinfecting method is to be used, contact Eppendorf AG to ensure that the intended method will not damage the device.
Safety Centrifuge 5418 / 5418 R English (EN) NOTICE! Damage to rotors from aggressive chemicals. Rotors are high-quality components which withstand extreme stresses. This stability can be impaired by aggressive chemicals. Avoid the use of aggressive chemicals, including strong and weak alkali, strong acids, solutions with mercury, copper and other heavy metal ions, halogenated hydrocarbons, concentrated saline solutions and phenol.
Safety Centrifuge 5418 / 5418 R English (EN) 3.5.5 Aerosol-tight centrifugation WARNING! Risk to health due to limited aerosol tightness with incorrect rotor/rotor lid combination. Aerosol-tight centrifugation is guaranteed only if the rotors and rotor lids intended for this purpose are used. For fixed-angle rotors the labeling always begins with FA, swing-bucket rotors are labeled with AT (aerosol tight).
Installation Centrifuge 5418 / 5418 R English (EN) 4 Installation 4.1 Selecting the location NOTICE! If an error occurs, the objects in the immediate proximity of the device will be damaged. In accordance with recommendations in EN 61010-2-020, leave a safety clearance of 30 cm around the device during operation. Please remove all materials and objects from this area. NOTICE! Damage from overheating. Do not install the device near to any heat sources (e.g., heating, drying cabinet).
Installation Centrifuge 5418 / 5418 R English (EN) 4.3 Installing the instrument NOTICE! Damage to electronic components due to condensation. Condensation can form inside the device after the device has been moved from a cool to a warmer environment. 5418: Wait for at least 3 hours before connecting the device to the power supply. Only 5418: Alternative: Let the device heat up for 30 minutes right before a brief transport.
Operation Centrifuge 5418 / 5418 R English (EN) 5 Operation 5.1 Overview of operating controls Familiarize yourself with the operating controls and the display before using the Centrifuge 5418 / 5418 R for the first time. Abb. 5-1: Control panel of Centrifuge 5418 and Centrifuge 5418 R. 1 3 2 5418 5 (1) 5418 R 4 Fig. 5-1: 2 4 (9) 5 9 8 8 6 7 6 3 Control panel of Centrifuge 5418 and Centrifuge 5418 R.
Operation Centrifuge 5418 / 5418 R English (EN) Abb. 5-2: Display of Centrifuge 5418 and Centrifuge 5418 R. 1 2 5418 Fig. 5-2: 3 7 1 4 6 5 5418 R 2 8 3 7 Display of Centrifuge 5418 and Centrifuge 5418 R. 1 Centrifugation time 5 Temperature (only 5418 R) 2 g-force (rcf) / speed (rpm) 5418 R: Set value x 1000 6 Status of the key lock (only 5418 R) : Centrifuging parameters cannot be modified unintentionally. : No key lock. 3 Status of centrifuge : Centrifuge lid unlocked.
Operation Centrifuge 5418 / 5418 R English (EN) 5.2.3 Loading the rotor CAUTION! Risk of injury due to asymmetric loading of a rotor. Load rotors symmetrically with identical tubes. Only load adapters with suitable tubes. Always use the same type of tubes (weight, material/density and volume). Check symmetric loading by balancing the adapters and tubes used with scales. CAUTION! Risk from damaged or overloaded tubes.
Operation Centrifuge 5418 / 5418 R English (EN) 1. Check to see if the outer sealing ring is correctly positioned in the groove. 2. Fit the rotor lid vertically onto the rotor. 3. Lock the rotor by turning the red rotor lid screw clockwise beyond an audible click until it can be turned no further. The rotor is correctly locked after the audible "click" is heard! 5.2.5 Closing the centrifuge lid WARNING! Crushing of the fingers with the centrifuge lid.
Operation Centrifuge 5418 / 5418 R English (EN) 5.3.4 FastTemp This function can be used to start a temperature control run directly without samples with a rotor and temperature-specific speed in order to quickly adjust the rotor chamber, incl. rotor and adapters, up to the set target temperature. Prerequisites • • • • The centrifuge is switched on. Rotor and rotor lid are correctly mounted. The centrifuge lid is closed.
Operation Centrifuge 5418 / 5418 R English (EN) 5.4 Centrifuging CAUTION! Risk from incorrectly-loaded rotors and damaged/overloaded tubes! Before commencing centrifugation, follow the safety instructions relating to risks from asymmetrically loaded and/or overloaded rotors and from overloaded, damaged and/or open tubes (see Warnings for intended use on p. 12). WARNING! Risk of injury from improperly attached rotors and rotor lids. Only centrifuge with rotor and rotor lid firmly tightened.
Operation Centrifuge 5418 / 5418 R English (EN) End of centrifugation • The centrifuge automatically stops after the set time has elapsed. The elapsed centrifugation will be shown in a blinking display during the braking process. A signal tone sounds when the rotor is at a standstill. • Only 5418: The centrifuge lid opens automatically. The display shows the symbol . • Only 5418 R: The centrifuge lid remains closed to maintain the sample temperature. You can open it by pressing the open key. 5.
Operation Centrifuge 5418 / 5418 R English (EN) 5.4.3.2 Performing short spin centrifugation 1. Only 5418 R: A short run at preselected g-force (rcf) or speed (rpm) can be set directly using the speed dial. 2. Only 5418 R: Use the temp arrow keys to adjust the temperature. 3. Start short run: Hold down the short key. • In the display flashes while the rotor is running. • The time is counted upwards in seconds. • During short run centrifuging all other keys are blocked. 4.
Maintenance Centrifuge 5418 / 5418 R English (EN) 6 Maintenance 6.1 Prepare cleaning/disinfection The procedure described in the following chapter applies to the cleaning as well as to the disinfection or decontamination. The table below describes the steps required on top of this: Cleaning Disinfecting/decontamination 1. Use a mild cleaning fluid to clean the accessible 1. Choose the disinfection method which surfaces of the device and the accessories. corresponds to the legal regulations and 2.
Maintenance Centrifuge 5418 / 5418 R English (EN) NOTICE! Damage from UV and other high-energy radiation. Do not use UV, beta, gamma, or any other high-energy radiation for disinfecting. Avoid storage in areas with strong UV radiation Autoclaving Rotors, rotor lids and adapters can be autoclaved (121 °C, 20 min). Replace the sealing ring in the lid groove of the aerosol-tight rotor lid after a maximum of 50 autoclaving cycles. Aerosol tightness Check that the seals are intact before use.
Maintenance Centrifuge 5418 / 5418 R English (EN) 5. Rinse the rotors and accessories thoroughly with distilled water. Rinse the rotor bores of fixed-angle rotors particularly thoroughly. Do not immerse the rotor in liquid as liquid can get trapped inside the cavities. 6. Place rotors and accessories on a cloth to dry. Place fixed-angle rotors with the rotor bores facing downwards to allow the bores to also dry. 7. Correctly reinsert the rotor lid sealing ring in the clean and dry groove. 8.
Maintenance Centrifuge 5418 / 5418 R English (EN) NOTICE! Glass breakage in the rotor chamber Glass tubes in the rotor chamber may break if the g-force is too high. Broken glass can damage the rotor, accessories and samples. Please note the manufacturer's information on the recommended centrifugation parameters (load and speed).
Troubleshooting Centrifuge 5418 / 5418 R English (EN) 7 Troubleshooting If you cannot remedy an error with the recommended measures, please contact your local Eppendorf partner. The contact address can be found online at: www.eppendorf.com/worldwide. 7.1 General errors Symptom/message Cause Remedy No display. No power connection. Check mains/power connection. No display. Power failure. Check the mains fuse of the device (see Fuses on p. 30). Check the mains fuse of the laboratory.
Troubleshooting Centrifuge 5418 / 5418 R English (EN) 7.2 Error messages If one of the following error messages appears, proceed as follows: 1. Remove fault (see Remedies). 2. If necessary, repeat centrifugation. Symptom/message Cause Remedy LID ERROR (5418)/ Centrifuge lid cannot be locked. LID (5418 R) Try again to close centrifuge lid. LID ERROR (5418)/ Centrifuge lid cannot be released. LID (5418 R) 1. Switch the centrifuge off and back on. 2. Press the open key.
Troubleshooting Centrifuge 5418 / 5418 R English (EN) Symptom/message Cause Remedy Error 19 (only for 5418 R Cooling circuit is overheated. Check unhindered air circulation Error 20 (only for 5418 R) Temperature sensor in rotor chamber is faulty. Switch centrifuge off and back on Error 21 (only for 5418 R) Temperature sensor on condenser is faulty. Switch centrifuge off and back on Error 24 (only for 5418 R) Cooling unit fault, e.g. overheated. Allow the centrifuge to cool down and 7.
Troubleshooting Centrifuge 5418 / 5418 R English (EN)
Transport, storage and disposal Centrifuge 5418 / 5418 R English (EN) 8 Transport, storage and disposal 8.1 Transport CAUTION! Bodily injury due to lifting and carrying heavy loads The device is heavy. Lifting and carrying the device can lead to back injuries. The device must be transported by least two people. Use a transport aid (e.g., dolly) to transport the device longer distances. Remove the rotor from the centrifuge before transport. Use the original packaging for transport.
Transport, storage and disposal Centrifuge 5418 / 5418 R English (EN)
Technical data Centrifuge 5418 / 5418 R English (EN) 9 Technical data 9.1 Power supply Centrifuge 5418 Mains connection 230 V, 50 Hz – 60 Hz 120 V, 50 Hz – 60 Hz 100 V, 50 Hz – 60 Hz Current consumption 1.4 A (230 V ) 2.8 A (120 V ) 3.4 A (100 V ) Power consumption Maximum 170 W EMC: Interference emission. (radio interference) EN 61326 – Class B (230 V) FCC15 - Class A (120 V/100 V) EMC: Noise immunity EN 61326 Overvoltage category II Fuses 2.5 AT (230 V) 5.
Technical data Centrifuge 5418 / 5418 R English (EN) 9.2 Ambient conditions Environment: Only for use indoors. Ambient temperature: Centrifuge 5418: 2 °C to 40 °C Centrifuge 5418 R: 15 °C to 35 °C Max. relative humidity: 75 %, non-condensing humidity. Atmospheric pressure: Use up to an altitude of 2 000 m above MSL. Degree of pollution: 2 9.3 Weight/dimensions Tab. 9-1: Centrifuge 5418 Dimensions: Width: 208 cm (8.19 in) Depth: 300 cm (11.8 in) Height: 210 cm (8.
Technical data Centrifuge 5418 / 5418 R English (EN) 9.4 Application parameters Start and stop times according to DIN 58 970 Rotor FA-45-18-11 5418 5418 R Voltage Acceleration times Deceleration times Voltage Acceleration times Deceleration times 230 V 120 V 100 V 16 s 16 s 18 s 18 s 18 s 18 s 230 V 120 V 100 V 13 s 13 s 13 s 13 s 13 s 13 s These values were calculated at 23°C.
Technical data Centrifuge 5418 / 5418 R English (EN)
Ordering information Centrifuge 5418 / 5418 R English (EN) 10 Ordering information 10.1 Centrifuge 5418 Order no. (International) 5418 000.017 - 10.2 022620321 022620304 Description Centrifuge 5418 with rotor FA-45-18-11 incl. rotor lid 230 V / 50 – 60 Hz 120 V / 50 – 60 Hz, with US-plug Centrifuge 5418 R Order no. (International) 5401 000.013 - 10.3 Order no. (North America) Order no. (North America) 5401000013 5401000137 Description Centrifuge 5418 R with rotor FA-45-18-11 incl.
Ordering information Centrifuge 5418 / 5418 R English (EN) 10.4 Fuses 10.4.1 Fuses for Centrifuge 5418 Order no. (International) Order no. (North America) 5425 351.003 5425 353.006 022668188 022668226 10.4.2 Description Fuse 2.5 A T (230 V), set of 2 5 A T (120 V), UL, set of 2 Fuses for Centrifuge 5418 R Order no. (International) Order no. (North America) 5425 351.003 5426 355.100 022668188 022668200 Description Fuse 2.5 A T (230 V), set of 2 6.
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