New Brunswick Biological Shakers Excella® E-25/25R Operating Manual M1353-0050 Revision D
COPYRIGHT: Copyright © 2011 New Brunswick Scientific Co., Inc., USA. No part of this publication may be reproduced without the prior permission of the copyright owner. New Brunswick Scientific reserves the right to change information in this document without notice. Updates to information in this document reflect our commitment to continuing product development and improvement. TRADEMARKS: Excella® and Innova® are trademarks owned and registered by New Brunswick Scientific Co., Inc., USA.
CAUTION! Risk of damage to personnel and/or equipment! This equipment must be operated as described in this manual. Please read the entire Operating Manual before attempting to use this equipment. If operational guidelines are not followed, equipment damage and personal injury can occur. Do not use this equipment in a hazardous atmosphere or with hazardous materials for which the equipment was not designed. New Brunswick Scientific Co., Inc.
WARRANTY New Brunswick Scientific equipment is protected by a comprehensive warranty. The warranty covers faulty components and assembly, and our obligation under this warranty is limited to repairing or replacing the instrument or part thereof, which shall prove to be defective after our examination.
TABLE OF CONTENTS 1 OVERVIEW.......................................................................................................................... 7 1.1 1.2 2 INSPECTION, VERIFICATION & UNPACKING OF EQUIPMENT........................ 11 2.1 2.2 2.3 2.4 2.5 3 STARTING THE SHAKER.................................................................................................. 22 CONTINUOUS (UNLIMITED) RUN ....................................................................................
9 REPLACEMENT PARTS & ACCESSORIES................................................................ 35 9.1 9.2 10 REPLACEMENT PARTS .................................................................................................... 35 ACCESSORIES ................................................................................................................. 35 DRAWINGS .................................................................................................................... 37 10.1 10.
1 OVERVIEW The Excella® E-25 Classic Incubator and E-25R Classic Refrigerated Incubator Shakers are large capacity shakers utilizing a UniCentric single eccentric counterbalanced drive to provide horizontal plane rotary motion in a 2.54 cm (1 in) circular orbit. A Proportional/Integral (PI) Microprocessor controller with instantaneous digital feedback controls the speed over a range of 50-400 rpm.
1.1 Specifications Excella E-25 & E-25R Incubator Shaker SHAKING Speed1 Control Accuracy Indication Stroke/Orbit TEMPERATURE Range (E-25) Range (E-25R) Control Accuracy Indication Ambient* Operating Environment Refrigerant (E-25R only) Alarms Timer Automatic Restart Drive Interrupt Electrical Requirements ETL Regulatory Standards CE Regulatory Standards Platform Overall Dimensions Chamber Dimensions 50-400 rpm 1 rpm 3-digit LED, displayed in 1 rpm increments 2.
1.2 Certifications The Excella E-25 & E-25R have been tested to ETL standards, to comply with UL and CAN/CSA electrical safety standards (see “ETL Regulatory Standards” in the specifications table, Section 1.1). As attested in the CE Declaration of Conformity reproduced on the following page, they also conform to the appropriate CE standards (see also “CE Regulatory Standards” in the specifications table).
M1353-0050 Excella® E-25/E25R Classic Incubator Shakers Operating Manual
2 2.1 INSPECTION, VERIFICATION & UNPACKING OF EQUIPMENT Inspection of Boxes After you receive your order from New Brunswick Scientific, inspect the boxes carefully for any damage that may have occurred during shipping. Report any damage to the carrier and to your local New Brunswick Sales Order Department. 2.2 Unpacking Save all packing materials and Operating Manual.
2.4 Out of Box Concerns If any part of your order was damaged during shipping, is missing pieces, or fails to operate properly, please contact your sales representative. 2.5 Warranty Registration Please register electronically at our Website: www.nbsc.
3 3.1 PREPARING THE LOCATION Physical Location It is essential that the instrument be situated in a area where there is sufficient space for the shaker and platform to clear walls and obstructions during operation. The surface on which the shaker is placed must be smooth, level, and able to support the shaker under full load operating conditions. CRUSH WARNING! Do not attempt to lift the E-25 or E-25R by hand. Always use a lifter or suitable equipment when raising the shaker.
In all cases, voltage variation must not exceed ± 10%. 3.4 Space Requirements Figure 1: Space Requirements 1 2 4 3 5 1 2 3 4 5 Height with lid open: 165.7 cm (65¼ in) 10 cm (4 in) front & left side clearance 127 cm (50 in) 87.
4 4.1 FEATURES Front Panel The keypad on the top (front) panel is the primary user interface to command the Excella E-10. This section will acquaint you with the keypad’s user interface keys, LED display, status indicators and function indicators.
4.1.1 LED Display The digital display on the control panel is a three-digit LED DISPLAY. During normal shaker operation, the display will indicate: Shaker status (On/Off) Shaking speed Chamber temperature Setpoints Hours remaining (in a timed run) Lid open (“LID”) 4.1.2 User Interface Keys As you can see in Figure 2a, the keypad has four user interface keys: START/STOP, , and SELECT. This is how they are used: START/STOP This key is used to start or stop the shaker.
HEAT Illuminates to indicate that the heater is on. TIME Indicates that the timer is in operation. The shaker can be programmed to run for a preset time from 0.1 to 99.9 hours. The timer can be disengaged without stopping an ongoing run. 4.1.4 Function Indicators Four function indicator lights are located to the right of the LED DISPLAY. They indicate the current parameter(s) being displayed: C Interior chamber temperature.
5 5.1 GETTING STARTED Installation of Platform A platform is a separate item that is required for operation. The E-25 and E-25R can be used with a wide variety of New Brunswick 46 x 76 cm (18 x 30 in) platforms, which will accept a variety of clamps for flasks test tubes, etc. See Section 9, Replacement Parts & Accessories, for details. A platform must be installed on the shaker prior to use.
5.2 Installing Flask Clamps Flask clamps purchased for use with universal platforms (see Section 9.2) require installation. Clamps are installed by securing the base of the clamp to the platform with the correct type and number of screws. All clamps are shipped complete with hardware. Clamps for 2-, 2.8- and 4-liter flasks are shipped with an additional girdle to keep the flasks in place. The girdle is an assembly of springs and sections of rubber tubing.
Figure 4b: Clamp Fastener NOTE: The upper girdle secures the flask within the clamp, and the bottom girdle keeps the flask from spinning. New Brunswick flask clamps are used on a variety of shaker platforms. Flat head screws of different lengths and thread pitch are used to secure the clamp, and all types are provided when you purchase clamps.
Figure 5: E-25 and E-25R Rear Panel 1 2 1 Power connector New Brunswick Scientific 2 Electrical specification plate Operating Manual
6 6.1 OPERATION Starting the Shaker To initially start the shaker, close the lid and turn the ON/OFF SWITCH on the right side panel (see Figure 2, repeated here for easy reference) to the ON position. Figure 2: Front Panel 1 2 1 Keypad & LED display 2 ON/OFF switch The shaking action may be stopped or started by pressing the START/STOP KEY. When the shaker begins to operate, the LED DISPLAY will track the speed as it accelerates to the last entered setpoint.
4. Set the speed by using the (UP) or (DOWN) KEY until the desired setpoint is displayed. Holding the (UP) or (DOWN) KEY will cause the setting to change more rapidly. NOTE: The setpoint may be changed during a run without stopping the shaker by following steps 2 - 4 above. During speed changes, a visual alarm (flashing RPM INDICATOR) will flash and an audible alarm will sound until the speed returns to within 5 rpm of the setpoint. 6.3 Checking a Setpoint 1.
If the shaker is stopped: 5. Press the START/STOP KEY. The shaker will start in untimed mode. 6. Press the START/STOP KEY again. The shaker will stop and the display will read OFF. 7. Press the START/STOP KEY a third time; the TIME INDICATOR will light and the shaker will start the timed run. To disable the visual alarm (flashing TIME INDICATOR), press the SELECT KEY and change to any other function. To cancel the timer while the shaker is running: 1. Press the SELECT KEY until the HRS indicator lights.
6.6 Temperature Setpoint Press the SELECT KEY until the function C INDICATOR illuminates. The temperature can be set from 5C above ambient temperature to 60C (non-refrigerated shakers) or from 4C to 60C (refrigerated shakers). Increasing or decreasing the setpoint is accomplished with the (UP) or (DOWN) KEY. Ambient temperature is measured one meter from the front of the shaker. During operation, if the temperature of the chamber is more than 1.
NOTE: The °C light will pulse rapidly to indicate it is not operating in the factory default mode. It will pulse for a longer duration and less rapidly (with a frequency of approximately one second) to indicate temperature is more than one degree above or below setpoint. To return to the factory calibration: 1. Press the SELECT KEY until the function C INDICATOR illuminates. 2. Simultaneously press the (UP) and (DOWN) KEYS. The display will indicate CAL. 3.
6.10 Maximum Recommended Speed Figure 6 shows the maximum recommended speed for your shaker, according to load. Figure 6: Load & Speed for E-25/E-25R 400 350 300 250 200 50, 125, 250 mL 20% Full 150 1L, 2L, 2.8L 20% Full 100 50 0 <30 30 to 36 36 to 40 40 to 60 >60 Platform and Load (lb) NOTE: In Figure 6, “20% Full” refers to the amount of liquid in the flasks. The platforms are fully loaded with flasks.
7 PREVENTIVE MAINTENANCE WARNING! Always turn off the shaker and disconnect the power cord from the power supply BEFORE performing any maintenance on the shaker. 7.1 Cleaning External Surfaces The shaker may be cleaned using a damp cloth or any standard, household or laboratory cleaner to wipe down its outer surfaces. Do not use abrasive or corrosive compounds to clean this instrument, as they may damage the shaker and void the warranty. 7.
4. Swing the ELECTRICAL PANEL to the right to access the PCB board that houses the fuse(s); see Figures 8a (E-25) and 8b (E-25R), where Item 1 identifies fuse holders— see Figures 8c & 8d for details.
Figure 8c: Fuse Holder Detail (E-25) 1 Figure 8d: Fuse Holder Detail (E-25R) 1 1 5. 6. 7. 8. Fuse Remove the fuse and check it. If it has failed, replace the fuse. Swing the ELECTRICAL PANEL to the left, flush against the shaker. Using the screws set aside, fasten the ELECTRICAL PANEL in place. With two hands, reinstall the top bezel, snapping the back in place first, then the front. Verify that it fits snugly and securely.
WARNING! The following procedures are provided for your information only. Do not attempt to perform these service interventions yourself unless you are an authorized service technician. 7.3 Belt Replacement To gain access to the drive belt, your service technician will follow these steps with reference to Figure 9: WARNING! Always keep fingers clear of the drive belt and pulley. 1. 2. 3. 4. Set the ON/OFF power switch to OFF. Disconnect the power cord from the electrical outlet. Open the lid.
Figure 9: Drive Belt Replacement 2 1 4 3 4 5 8 6 7 1 2 3 4 Upper bearing housing E-ring Flexure arm Flexure arm nuts 5 6 7 8 Counterweighted pulley Drive belt Motor mounting bracket Motor pulley 14. Use the hex wrench to tighten the four hex nuts on the motor mounting bracket. 15. Reinstall the upper bearing housing on the shaft. 16. Reattach the flexure arms to the bearing housing mounting plate with the four nuts previously removed. 17. Reattach the E-ring to the shaft. 18.
8 TROUBLESHOOTING If any problems occur with your shaker, do not attempt to perform any service on the shaker other than specified in this manual. Unauthorized servicing may void the warranty. Please contact your local New Brunswick Sales Order Department In any correspondence with New Brunswick, please refer to the Model Number and Serial Number of your shaker. This information is on the ELECTRICAL SPECIFICATION PLATE, located on the rear panel of the shaker.
Symptom(s) Operating noise Incubator does not reach set temperature. Incorrect temperature indication. Probable Cause(s) & Solution(s) Load out of balance: unload all contents, then reload. Loose component(s) in platform, subplatform and/or drive assembly: call for service. Heater fuse blown: replace. Compressor fuse blown: replace. Compressor over-pressure switch activated: call for service. Ambient temperature too high or too low: cool or heat the room as needed. Defective heater: call for service.
9 REPLACEMENT PARTS & ACCESSORIES When ordering replacement or accessory parts, or requesting service information, please provide the Model Number and Serial Number of your shaker. This information is on the ELECTRICAL SPECIFICATION PLATE, located on the side panel of the shaker. 9.1 Replacement Parts Table 2: Service Parts Part Description 8.0A Fuse (Motor) 15A Circuit Breaker 120V 15A Power Cord 220V Power Cord V Belt Gasket, Door AC Connector, Power Entry Drive Assembly 9.
Table 4: Universal Platform Flask Capacity Flask Type 50 ml Erlenmeyer Flasks 125 ml Erlenmeyer Flasks 250 ml Erlenmeyer Flasks 500 ml Erlenmeyer Flasks 1 L Erlenmeyer Flasks 2 L Erlenmeyer Flasks 2.8 L Fernbach Flasks 4 L Erlenmeyer Flasks 6 L Erlenmeyer Flasks Capacity 92 39 30 18 12 8 6 6 4 Table 5: Carriers & Test Tube Racks Accessory Description Part Number Utility Tray with rubber mat for shaking 96 well plates, petri dishes and other labware at low speeds.
10 DRAWINGS Figure 10: E-25 & E-25R Control Schematic (Overview) NOTE: For a larger version of this drawing, contact your sales or service representative.
Figure 11a: 230V ac, 50 Hz Power Schematic 230Vac/50Hz OPERATION POWER TRANSFORMER WHITE GRAY BLACK RED N 100V 120V 230V M1324-8015 FROM M1324-7003 TB001 RED COND. FAN ORANGE M1324-8017 BLACK WHITE YELLOW M1324-8016 VIOLET OVER PRESSURE SWITCH COMPRESSOR UNIT J4-1 J4-3 J4-5 J4-2 J4-4 J4-6 M1324-8017 Figure 11b: 100V ac, 60 Hz Power Schematic 100Vac/60Hz OPERATION POWER TRANSFORMER WHITE GRAY BLACK RED N 100V 120V 230V M1324-8015 TB001 FROM M1324-7003 ORANGE COND.
Figure 11c: 120V ac, 60 Hz Power Schematic 120Vac/60Hz OPERATION TB001 FROM M1324-7003 RED COND. FAN ORANGE M1324-8017 BLACK WHITE YELLOW M1324-8016 VIOLET OVER PRESSURE SWITCH COMPRESSOR UNIT J4-1 J4-3 J4-5 J4-2 J4-4 J4-6 M1324-8017 Figure 11d: 100V ac, 50 Hz Power Schematic 100Vac/50Hz OPERATION POWER TRANSFORMER WHITE GRAY BLACK RED N 100V 120V 230V M1324-8015 TB001 FROM M1324-7003 ORANGE COND.
Figure 12: E-25R Refrigeration Schematic 10.
10.
11 A Accessories · 35 Actual Temperature · 25 Alarm Functions · 24 Ambient Temperature · 7 Audible Alarm Disabling · 24 C Calibrating Speed · 26 Carriers · 36 CE Standards · 10 Certifications · 9 Checking Setpoints · 23 Clamp Hardware · 20 Cleaning External Surfaces · 28 Contact New Brunswick How to · 2 Continuous Run · 23 Copyright Notice · 3 Customer Satisfaction Form 6300 · 12 D Declaration of Conformity · 10 Dedicated Platforms · 35 Disclaimer Notice · 3 Double Girdle Clamps · 19 Drawings Index o
Regulatory Standards · 9 Relative Humidity · 13 Replacement Parts · 35 S Service Parts · 35 Shaking Speed Maximum Recommended · 27 Shipping Damage · 12 Specifications · 8 Speed Calibration · 26 Starting the Shaker · 22 Status Indicators · 16 T Table of Contents · 5 Tables Index of · 41 Temperature Correction Value · 25 Temperature Offset Calibration · 25 Temperature Setpoint · 25 Test Tube Racks · 36 New Brunswick Scientific Timed Functions · 23 Timer Cancelling the · 24 Troubleshooting · 33 U Univ
M1353-0050 Excella® E-25/E25R Classic Incubator Shakers Operating Manual
Your local distributor for New Brunsdwick products: www.nbsc.com/ContactUs New Brunswick Scientific, 175 Freshwater Boulevard, Enfield, CT 06082-4444 USA Eppendorf AG 22331 Hamburg Germany Tel: +49 40 538 01-0 Fax: +49 40 538 01-556 E-mail: eppendorf@eppendorf.com New Brunswick Scientific Europe B.V. Nijmegen The Netherlands Tel: +31 (0) 24 3717 600 E-mail: europe@nbsbv.nl Eppendorf North America, Inc. Hauppauge, NY USA Tel: +1 516 334 7500 +1 800 645 3050 E-mail: info@eppendorf.
