Instruction Manual

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10 Technical data
BioPhotometer plus — Operating manual
10.5 Photometer
10.6 Other technical parameters
Measuring principle Absorption single beam photometer with reference
beam and several fixed wavelenghts
Light source Xenon flash light
Monochromator Holographic concave grating
Beam receiver Silicon photo diodes
Wavelengths 230, 260, 280, 340, 405, 490, 550, 595, 650 nm
Wavelength selection Dependent on method, controlled by program
Spectral bandwidth 5 nm at 230 to 340 nm
7 nm at 405 to 650 nm
Systematic wavelength error ± 1 nm at 230 to 280 nm
± 2 nm at 340 to 650 nm
Photometric measuring range Quartz glass cuvette:
• A = 0 to 3, except:
• A = 0 to 2 at 340 nm
• Only for dye methods: 
A = 0 to 2 at 550/650 nm
UVette (Eppendorf):
• A = 0 to 2.5 at 230 nm
• A = 0 to 2.6 at 260 nm
• A = 0 to 2.8 at 280 nm
• Other values see quartz glass cuvette
Reading accuracy ΔA = 0.001
Random photometric error  0.002 at A = 0
 0.005 at A = 1
Systematic photometric error ± 1% at A = 1
Stray light component < 0.05 %
Cuvette material For DNA, RNA, Oligo, Protein UV, Assay 340:
Quartz glass or UV transparent plastic 
(UVette by Eppendorf)
For OD600, Bradford, Lowry, BCA, Assay 405,
Assay 490:
Glass or plastic
Cuvette shaft 12.5 mm x 12,5 mm, not temperature-controlled
Overall cuvette height Min. 36 mm
Height of the light beam in the cuvette 8.5mm
Light beam in the cuvette Width: 1 mm
Height: 1.5 mm
Keyboard 19 foil keys
Display Illuminated graphic display, 33 mm x 60 mm
Operator guidance language English, French, German
Result output Via display and printer:
Absorbance, concentration, ratio, FOI
Standards and regulations According to VDE, CE, IEC 1010-1
EN
Operating manual