New Brunswick Water Bath Shaker C-76 Classic Operating Manual M1248-0051 Revision G
COPYRIGHT: Copyright © 2013 Eppendorf AG, Hamburg. No part of this publication may be reproduced without the prior permission of the copyright owner. The company reserves the right to change information in this document without notice. Updates to information in this document reflect our commitment to continuing product development and improvement. TRADEMARKS: Eppendorf® is a registered trademark, and New Brunswick™ and the New Brunswick Logo™ are trademarks of Eppendorf AG, Hamburg, Germany.
WARNING! This equipment must be operated as described in this manual. If operational guidelines are not followed, equipment damage and personal injury can occur. Please read entire Operating manual before attempting to use this unit.
TABLE OF CONTENTS 1 OVERVIEW .......................................................................................................................................................6 1.1 2 INSPECTION, VERIFICATION & UNPACKING OF EQUIPMENT ........................................................9 2.1 2.2 2.3 3 INSPECTION OF BOXES .....................................................................................................................................9 PACKING LIST VERIFICATION .............
8 TROUBLESHOOTING...................................................................................................................................34 9 REPLACEMENT PARTS & ACCESSORY INFORMATION.................................................................35 9.1 REPLACEMENT PARTS ....................................................................................................................................35 9.2 ACCESSORIES .......................................................................
1 OVERVIEW The C-76 Classic Water Bath Shaker is a shaker utilizing an eccentric counterbalanced drive to provide horizontal plane rotary motion in a 1/2” (1.3 cm) circular orbit. A Proportional/Integral (PI) Microprocessor controller with instantaneous digital feedback controls the speed over a range of 50-300 RPM. It also provides temperature control over a range of 7°C above ambient to 80°C. For lower temperatures, an optional water bath cooling coil is available.
1.1 Specifications C-76 Classic Water Bath Shaker Speed • Control Accuracy • Indication • Stroke Temperature Control Indication Heaters Ambient Operating Environment Alarms Timer Automatic Restart Electrical Requirements Platform Overall Dimensions Weight 50-300 rpm ± 2 rpm 3 Digit LED in 1 rpm increments ½ inch (1.3 cm) 7°C above ambient (or cooler temperatures with optional cooling coil) to 80°C* ± 0.25°C Digital LED display in 0.
M1248-0051 C-76 Classic Water Bath Shaker Operating manual
2 2.1 INSPECTION, VERIFICATION & UNPACKING OF EQUIPMENT Inspection of Boxes After you receive your order from Eppendorf, inspect the boxes carefully for any damage that may have occurred during shipping. Report any damage immediately to the carrier and to your local Eppendorf Sales Order Department. 2.2 Packing List Verification Verify against your packing list that you have received the correct materials. 2.3 Unpacking of Equipment Save all packing materials and the Operating manual.
3 3.1 PREPARING THE LOCATION Physical Location It is essential that the instrument be situated in an area where there is sufficient space for the shaker and platform to clear walls and potential obstructions during operation. The surface on which the unit is placed must be smooth, level, and able to support the shaker under full load operating conditions. 3.
3.3 Drain Valve Connection The unit should be placed in close proximity to an open drain. It is recommended that a 3/8” ID hose be connected to the DRAIN VALVE for draining the bath. The DRAIN VALVE is located near the rear of the unit, on the side opposite the ON/OFF SWITCH (see Figure 1 below). Figure 1: Top View DRAIN VALVE ON/OFF SWITCH 3.4 Cooling Coil Connections If your water bath is equipped with an optional COOLING COIL, prepare the coil for operation: 1.
Figure 2: Water Bath Assembly Rear Panel 2. Knock out the two plugs on the center of the two grommets on the rear panel (see Figure 3).
3. Slip two lengths of 3/8 inch (9.5 mm) ID hose with hose clamps over the coil ends. The hoses should be long enough to suit water or coolant supply and return (drain) length requirements. Be careful to note or label the operation of each hose. 4. Allow 1/8 inch (3 mm) to 3/16 inch (5 mm) of hose to extend beyond the hose clamps and secure in place. Ensure that the clamps do not damage the hose. 5.
4 FEATURES 4.1 Keypad FUNCTION INDICATORS STATUS INDICATORS MAINT RPM SET HRS TIME ºC MUTE I LED DISPLAY START STOP s t SELECT USER INTERFACE KEYS 4.1.1 LED Display The digital display on the control panel is a-three digit LED DISPLAY. During normal shaker operation the display will indicate: • • • • • 4.1.2 Shaker status (on/off) Shaking speed Chamber temperature Setpoints Hours remaining (timed run) User Interface Keys • START/STOP This key is used to start or stop the shaker.
• SELECT • σ(UP), τ(DOWN) This key is used to change the displayed parameter. These keys are used to increase or descrease the setpoint of a displayed parameter. They also allow the user to enter the SET MODE for setpoint changes. 4.1.3 Status Indicators Four status indicator lights are located to the left of the LED DISPLAY: • MAINT Remains lit after 10,000 hours of use. Accumulated running time is internally monitored and may be displayed as a guideline.
4.2 Platform Assemblies The C-76 can be used with a wide variety of New Brunswick 12-inch X 16.5-inch (31 X 42 cm) platforms, which will accept a variety of clamps for flasks, test tubes, etc. A platform, which is a separate item, is required for operation. Refer to the Replacement Parts and Accessory Information section of this manual for details. 4.
4.4 Optional Cooling Coil The optional COOLING COIL enables the shaker to maintain accurate temperature control within 7°C of coolant temperature to 80°C. 4.5 Optional Gable Covers The optional GABLE COVERS, available in PLEXIGLAS and STAINLESS STEEL, are designed to maintain culture sterility by allowing condensation from the bath to run down the sides of the cover, and not drip directly on the contents of the bath.
5 5.1 GETTING STARTED Electrical Connections CAUTION! Before making electrical connections, verify that the power source voltage matches the voltage on the electrical specification plate and the on/off switch is on the OFF position. The electrical specification plate is located on the side panel of the unit near the on/off switch. Also verify that the power cord plug is compatible with your outlet.
5.2 Installation of Drive Arms and Platform The SUSPENSION ARM KIT included in your shipment allows you to install a platform in the bath. The kit includes a set of four each DRIVE ARMS, KNOBS, LOCKWASHERS and PLATFORM SCREWS. The DRIVE ARMS attach to the SUSPENSION ARM and are secured by a KNOB and LOCKWASHER (see Figure 6 below). The platform rests on the DRIVE ARMS inside the bath and is secured by the PLATFORM SCREWS. The DRIVE ARMS and PLATFORM can be installed separately or as an assembly.
Figure 7: Platform Screw Locations DRIVE ARMS (4) Figure 8: Platform Installation M1248-0051 C-76 Classic Water Bath Shaker Operating manual
NOTE: For ease and convenience, an additional Suspension Arm Kit (M12480730) is available. A platform can also be installed by securing it to the drive arms, and then securing the entire assembly to the unit. Verify that the arms are secured in place before operating the unit. 5.3 Installing Flask Clamps Flask clamps purchased for use with universal platforms (see Section 9.2) require installation.
3. After making sure the sections of tubing are located between the clamp legs, roll the first girdle down the legs of the clamp as far as it can go. The tubing sections will rest against the platform, and the springs will be under the clamp base. 4. Place the second girdle around the upper portion of clamp body (just as the first girdle was initially). Make sure that its spring sections rest against the clamp legs, while its rubber tubing sections sit against the flask, in between the clamp legs.
The minimum water level required for operation is ½ inch (12.7 mm) above the top of the PLATFORM. For most applications, the maximum allowable level of water in the bath is 2 inches (5 cm) above the top of the PLATFORM. The maximum level is measured with flasks in the bath.
6 6.1 OPERATION Starting the C-76 To initially start the shaker, turn the ON/OFF SWITCH on the side of shaker to the ON position. If the Shaker begins to operate, the LED DISPLAY will track the speed as it accelerates to the last entered setpoint. The shaking action may be stopped or started by pressing the START/STOP KEY. 6.2 Continuous (Unlimited) Run 1. Press SELECT until the RPM INDICATOR is illuminated. 2. If the display indicates that the shaker is OFF, press the START/STOP KEY. 3.
CAUTION! Holding the σ or τ key for more than 0.5 seconds will cause the speed setpoint to change. Should this occur, resetting will be necessary. 6.4 Timed Functions The shaker can be programmed to stop automatically after a preset time period of 0.1 hour to 99.9 hours. There must be power to the shaker in order to set the timer; however, a timed run can be initiated while the unit is either shaking or stopped. 6.4.1 Setting the Timer To set the time: 1.
6.5 Alarm Functions The shaker has a visible alarm (flashing TIME INDICATOR) that is activated (a) at the end of a timed run, or (b) if the temperature is 1ºC or more from the setpoint, or if the speed is 5 RPM or more from the setpoint. It may be deactivated in the following way: 1. Press the SELECT KEY until the HRS INDICATOR is illuminated. 2. Simultaneously press the σ and τ KEYS. The SET and MAINT INDICATORS will flash. 3. While the SET and MAINT INDICATORS are flashing, press the START/STOP KEY.
3. The SET and MAINT INDICATORS will flash and the accumulated running time will be displayed in hundreds of hours (i.e., “02” equals 200 hours; “102” equals 10,200 hours). This display will continue for 10 seconds and then default to the previous mode readout. 6.9 Maint Indicator After 10,000 hours of operation, the MAINT INDICATOR will illuminate. Preventive maintenance is recommended at this point. To deactivate the MAINT INDICATOR, follow these steps: 1.
7 PREVENTIVE MAINTENANCE WARNING! Always turn off the shaker and disconnect the power cord from the power supply before performing maintenance on the unit. 7.1 Cleaning Surfaces Clean the exterior surfaces of the unit with a damp cloth. You may also use any standard household or laboratory cleaner. Do not use abrasive or corrosive compounds, as they may damage the unit and void the warranty.
Figure 5: Shaker Rear Panel FUSE HOLDER POWER CONNECTOR 1. Insert a small flat-bladed screwdriver into the fuse holder groove and turn counterclockwise until it disengages. The fuse holder will spring free. Figure 10: Fuse Holder Detail 2. Check the fuse and replace it if necessary.
7.3 Belt Replacement To replace the drive belt of the shaker: WARNING! The following procedure should only be performed by an NBSauthorized service technician. WARNING! Always turn off the shaker and disconnect the power cord from the power supply before changing the belt. 1. Allow sufficient time for the water bath to cool, then open the DRAIN VALVE and allow the bath to drain. Close the DRAIN VALVE and remove the drain hose if attached. 2.
4. Slide the water bath assembly to the front of the unit and tilt the assembly to gain access to the heater wires. Label or make a note of the location of each wire, then detach them from the terminal block. 5. Lift and remove the water bath assembly from the unit and set it aside. Figure 12: Top View without Water Bath Assembly TERMINAL BLOCK SUSPENSION ARM 6. Remove the four Phillips head screws (shown) that hold the SUSPENSION ARM in place, and remove the SUSPENSION ARM from the unit.
Figure 13: Top View, Lower Mover Plate Removed SUBPLATFORM ATTACHING HARDWARE (BOTH SIDES) LOWER MOVER PLATE 7. Loosen and remove the hardware that attaches the UPPER FLEXURES to LOWER MOVER PLATE.
8. Using your fingers, reach under the SUBPLATFORM and move the belt so that it falls off the drive pulley. Remove the belt from the bearing housing pulley. Figure 15: Belt Removal BELT DRIVE PULLEY BEARING HOUSING PULLEY LOWER MOVER PLATE 9. Grab the belt from underneath the SUBPLATFORM, pull it forward and over one corner of the SUBPLATFORM. Repeat for the other corner, until the belt clears the SUBPLATFORM, but is constrained by the LOWER MOVER PLATE. 10.
8 TROUBLESHOOTING If any problems occur with your shaker, do not attempt to perform any service on the unit other than specified in this manual. Unauthorized servicing may void the warranty. Please contact your local Eppendorf Sales Order Department In any correspondence with Eppendorf, please refer to the Model Number and Serial Number of your unit. This information is on the ELECTRICAL SPECIFICATION PLATE, which is located on the side panel of the unit, near the ON/OFF SWITCH.
9 REPLACEMENT PARTS & ACCESSORY INFORMATION When ordering replacement or accessory parts, or requesting service information, please provide the Model Number and Serial Number of your shaker. This information is on the ELECTRICAL SPECIFICATION PLATE, which is located on the side panel of the unit. 9.1 Replacement Parts Part Description V-Belt Fuse, Slo Blo 0.5 A, 250 V 9.
9.2.2 Test Tube Racks Accessory Description Per Bath Test Tube Rack for 13 mm test tubes* Test Tube Rack for 16 mm test tubes* Test Tube Rack for 20 mm test tubes* Test Tube Rack for 25 mm test tubes * 4 4 4 4 Per Rack Part Number 60 36 29 18 AG7-TT13 AG7-TT16 AG7-TT20 AG7-TT25 * Requires Sub-Platform M1239-9939 9.2.
10 DRAWINGS Figure 16: Control Schematic, 100-130 VAC 50/60 Hz New Brunswick Operating manual
Figure 17: Control Schematic, 200-240 VAC 50/60 Hz M1248-0051 C-76 Classic Water Bath Shaker Operating manual
11 A Accessory Descriptions and Part Numbers · 36 Alarm Functions · 26 B Baffles · 16 Installation · 16 Belt Replacement · 32, 33 Replacement · 30 C Clamp Hardware · 22 Cleaning Surfaces · 28 Continuous Run · 24 Cooling Coil · 12, 17 Connecting · 12 D Double Girdle Clamps · 21 Drain Valve · 30 Connecting · 11 Drive Arms · 19, 20, 21, 30 Installation · 19 Knobs · 19, 20, 21 Lockwashers · 19, 21 E Electrical Connections · 18 Environment · 10 Equipment Inspection Of · 9 Unpacking Of · 9 F Flask Clamps
U Unlimited Run · 24 Upper Flexures · 32, 33 User Interface Keys · 14 M1248-0051 C-76 Classic Water Bath Shaker W Water Bath Removal · 30 Operating manual
New Brunswick Operating manual
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