User Manual

Product description
Eppendorf Cell Culture Consumables
English (EN)
5
2.2 Flasks
The flasks are suitable for centrifugation (see Technical data on p. 7).
2.2.1 Filter cap
When fully closed, flasks with a filter cap are suitable for the growth of cells as the gas
exchange takes place continuously via the filter.
2.2.2 Plug-seal cap
The flasks can be completely closed with the plug-seal cap.
To incubate the cells, turn the cap to the vent position (Vent). Securely engaging the cap
in the Vent position ensures that the cells are properly vented.
•Prevention of the edge effect:
The 96-well plate has a peripheral moat
which can be filled prior to cell seeding
in order to prevent the edge effect.
Before filling the cells into the wells,
pipette 5 mL water/buffer into the
peripheral moat.
In order to make the filling of the moat
easier, you can reduce the surface
tension of the liquid by adding serum,
proteins or detergent. Additional
information is available in Eppendorf
Application Note 326.
In addition, it is also possible to fill the inter-well space within the plate with 8 to 10 mL
of liquid. This delays the cooling of the medium in the wells when the plate is used
outside of the incubator.
•Additional wells:
For the growth of very small numbers of
cells, the 6-well plate has 2 small,
additional wells (1) at the center.
These wells can be used for a direct
sample preparation on the plate (e.g.
preparing of coloring or antibodies
solutions).
1