on see Operating CellTram® p. Tab. Fig. —p. manual Air · Oil · vario Operating manual 5176 900.
Copyright © 2012 Eppendorf AG, Hamburg. No part of this publication may be reproduced without the prior permission of the copyright owner. CellTram, Eppendorf and TransferTip are registered trademarks of Eppendorf AG, Hamburg, Germany. Registered trademarks are not marked in all cases with ™ or ® in this manual. 5176 900.
CellTram® Air · Oil · vario — Operating manual Table of contents 1 Table of contents 1 Operating instructions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1.1 Using this manual. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1.2 Danger symbols and danger levels . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1.2.1 Danger symbols . . . . . . . . . . . . . . . .
CellTram® Air · Oil · vario — Operating manual 4 5 Operation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20 5.1 Holding and transferring cells . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20 5.2 Suctioning liquid . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 21 6 Maintenance. . . . . . . . . . . . . . . . . . . . . . . . . .
CellTram® Air · Oil · vario — Operating manual 1 Operating instructions 1 Operating instructions 1.1 Using this manual Read this operating manual completely before using the device for the first time. Please also note the operating instructions for the accessories, if applicable. This operating manual is part of the product. Please keep it in a place that is easily accessible. Enclose this operating manual when transferring the device to third parties.
CellTram® Air · Oil · vario — Operating manual 2 2 2.1 Product description Product description Main illustration 2.1.1 CellTram Air Abb. 1:Side view 1 Fig. 1: 2 3 4 5 6 7 8 Side view 1 Rotary knob 2 Scale (from 0 to 9) 3 Tilting joint 4 Piston window 5 Foot 6 Ventilation screw 7 Barb screw 8 Pressure tubing Abb. 2:Top view 1 2 15 10 5 0 Fig.
CellTram® Air · Oil · vario — Operating manual 2.1.2 CellTram Oil Abb. 3:Side view 1 2 3 4 5 6 7 8 9 10 Fig. 3: Side view 1 Pressure tubing 2 Barb screw 3 Cylinder body (transparent) 4 Filling bore with filling screw 5 Piston (in initial position) 6 Housing 7 Scale 8 Rotary knob 9 Tilting joint 10 Foot 2.1.3 CellTram vario Abb. 4:Side view 1 2 3 4 5 6 7 8 9 10 11 Fig.
CellTram® Air · Oil · vario — Operating manual 2.1.4 Universal capillary holder Abb. 5:Universal capillary holder 1 Fig. 5: 2 3 4 5 Universal capillary holder 1 Barb 2 Rear knurled screw 3 Tension piece 4 Front knurled screw 5 Grip head 0 for capillaries with an outer diameter of 1.0 to 1.1 mm 2.1.5 Grip head Abb. 6:Grip head 0 with O-rings and sealing washer Fig.
CellTram® Air · Oil · vario — Operating manual 2.2 Features The CellTram Air, CellTram Oil and CellTram vario are manual piston pumps. Using a movable piston, the CellTram generates differences in pressure which are transferred directly to a capillary via a pressure tube. The piston is moved in the CellTram using the dial. A clockwise rotation, i.e. to the right, results in an increase in pressure, the capillary contents are pressed out. A counterclockwise rotation, i.e.
CellTram® Air · Oil · vario — Operating manual 2.3 Delivery package 2.3.1 Quantit y Order no. (international) Description 1 5176 000.017 CellTram Air 1 5176 114.004 Pressure tube 1m 5176 190.002 Universal capillary holder For holding Femtotips, Femtotips II or grip heads Including grip head 0, adapter for Femtotips and spare O-rings 1 - Allen key (4 mm) 1 5176 900.018 Operating Manual CellTram Air/oil/vario 1 2.3.2 CellTram Oil Quantit y Order no.
CellTram® Air · Oil · vario — Operating manual Quantit y Order no. (international) Description 1 - Quick valve system For simple filling of the CellTram Consists of a premounted filling screw, disposable syringe and filling tube with a Luer Lock connector 1 - Bottle with mineral oil 25 mL 1 - Allen key (4 mm) 1 5176 900.
CellTram® Air · Oil · vario — Operating manual 3 3 3.1 Safety Safety User profile The device and accessories may only be operated by trained and skilled personnel. Before using the device, read the operating manual carefully and familiarize yourself with the device's mode of operation. 3.2 Intended use The CellTram has been designed and manufactured for use in the field of Intracytoplasmic sperm injection (ICSI) in conjunction with Eppendorf VacuTips and Transfertips.
CellTram® Air · Oil · vario — Operating manual CAUTION! 3.4 CAUTION! Poor safety due to incorrect accessories and spare parts. The use of accessories and spare parts other than those recommended by Eppendorf may impair the safety, functioning and precision of the device. Eppendorf cannot be held liable or accept any liability for damage resulting from the use of incorrect or non-recommended accessories and spare parts, or from the improper use of such equipment.
CellTram® Air · Oil · vario — Operating manual 4 4 4.1 Installation Installation Selecting the location Please select the location according to the following criteria: • Stable • Level • Horizontal • Vibration-damped 4.2 Mounting the CellTram Valid for: • CellTram Air • CellTram Oil • CellTram vario Abb. 8:ExampleCellTram Air 1 Fig. 8: 2 ExampleCellTram Air 1 Pressure tubing 2 Universal capillary holder For holding Eppendorf capillaries (e.g.
CellTram® Air · Oil · vario — Operating manual 4.3 Filling oil Valid for: • CellTram Oil • CellTram vario Hint! Hint! Hint! Work with an air cushion in the visible area of the capillary in order to avoid contact between the oil and the medium. Observe the legal regulations for purity of the oil. The supplied oil is generally used in the ICSI area to coat drops containing embryos / egg cells or sperm. The supplied oil is not sterile.
CellTram® Air · Oil · vario — Operating manual 4.3.1 Heating the syringe 1. Screw the filling hose on to the tip. 2. Aspirate at least 3 mL of oil into the syringe. 4.3.2 Connecting the filling tube 1. Turn the rotary knob to move the piston to the initial position. 2. Unscrew the blind screw. 3. Screw the filling tube in the filling bore. 4.3.3 Filling the piston chamber and universal capillary holder with oil Prerequisites The piston is in the starting position. 1. Hold the CellTram vertically. 2.
CellTram® Air · Oil · vario — Operating manual 4. Completely fill the piston chamber with oil. Make sure that there are no air bubbles in the tip of the piston chamber. 5. Completely fill the universal capillary holder with oil. 6. Visually check that no air bubbles remain in the universal capillary holder. 4.3.4 Sealing the filling bore 1. Position the CellTram horizontally. 2. Unscrew the filling tube. 3. Fill the filling bore with oil.
CellTram® Air · Oil · vario — Operating manual 4. Screw on the blind screw. When doing so, make sure that no new air bubbles enter the bore. 5. Soak up any excess oil using a soft cellulose cloth. 4.3.5 Removing air bubbles 1. If there are still air bubbles in the piston chamber: slant the CellTram to allow air bubbles to escape to the filling bore. Remove the air bubbles. 4.4 WARNING! CAUTION! Hint! 18 Mounting the capillary WARNING! Risk of injury due to flying capillaries and glass splinters.
CellTram® Air · Oil · vario — Operating manual 1. Slightly screw the grip head into the universal capillary holder. 2. Slide the capillary through the inner O-rings up to the stop into the universal capillary holder. No stop is felt with capillaries with an inner diameter < 0.8 mm. Hint! 3. Tighten the grip head. 4. In the case of CellTram Oil and CellTram vario, carefully wipe any excess oil off of the universal capillary holder and the capillary using a soft cellulose cloth if necessary.
CellTram® Air · Oil · vario — Operating manual 5 5 5.1 Hint! Operation Operation Holding and transferring cells CellTram Air: An overpressure or vacuum can be built up in the capillary using the volume of the piston chamber (cylinder) and the pressure tube. Therefore, the pressure behavior varies depending on the position of the piston. If the piston is located, e.g., in position 10 to 15, the differences in pressure are greater than, e.g., in position 0 to 5.
CellTram® Air · Oil · vario — Operating manual 5.2 Suctioning liquid Valid for: • CellTram Air 1. Dip the capillary into the liquid that is to be aspirated. 2. Turn the rotary knob counterclockwise to the left (by approx. 2 revolutions) to aspirate the liquid by creating a low pressure. 3. Wait for a short period and then loosen the ventilation screw. 4. Retighten the ventilation screw after pressure compensation.
CellTram® Air · Oil · vario — Operating manual 6 6 6.1 Maintenance Maintenance Replacing O-rings If the O-rings on the universal capillary holder or on the grip head are damaged, they can be replaced. 1. Remove the O-rings and the sealing washer with the extraction tool. 2. Insert the 2 new O-rings and a new sealing washer in the correct order. 3. Make sure that the 2 sealing washers and 2 matching O-rings are correctly arranged in the grip head. 6.
CellTram® Air · Oil · vario — Operating manual Required equipment • Alcohol (ethanol, isopropanol) or disinfectants containing alcohol Proceed as follows: 1. Choose the disinfection method which corresponds to the legal regulations and guidelines in place for your range of application. 2. Wipe all surfaces of the CellTram and accessories using the disinfectant. 3. Flush the pressure tube and universal capillary holder with the disinfectant if required. 4.
CellTram® Air · Oil · vario — Operating manual 7 7 7.1 7.1.1 Technical data Ambient conditions CellTram Air Ambience For indoor use only Ambient temperature max. 40°C Max. relative humidity Max. 80% Maximum pressure 2 900 hPa 7.1.2 CellTram Oil Ambience For indoor use only Ambient temperature max. 40°C Max. relative humidity Max. 80% Maximum pressure Approx. 20 000 hPa 7.1.3 24 Technical data CellTram vario Ambience For indoor use only Ambient temperature max. 40°C Max.
CellTram® Air · Oil · vario — Operating manual 7.2 Weight/dimensions 7.2.1 CellTram Air Footprint (w/o pressure tubing) 175 mm × 60 mm Weight Approx. 1.4 kg Rotary knob diameter 40 mm 7.2.2 CellTram Oil Footprint (w/o pressure tubing) 275 mm × 60 mm Weight Approx. 1.7 kg Rotary knob diameter 40 mm 7.2.3 CellTram vario Footprint (w/o pressure tubing) 29.5 x 6 cm Weight Approx. 1.7 kg Rotary knob diameter (coarse) 42 mm Rotary knob diameter (fine) 34 mm 7.2.
CellTram® Air · Oil · vario — Operating manual 7.3 7.3.1 CellTram Air Change in volume (per rotary knob revolution): 88 μL Minimum adjustment volume < 0.2 μL Adjustable total volume 2.640 μL Entire stroke of the piston 15 mm (corresponds to 30 revolutions) Generation of pressure Using piston and cylinder 7.3.2 CellTram Oil Change in volume (per rotary knob revolution): 9,6 μL Minimum adjustment volume: < 0.02 μL Adjustable total volume 960 μL Total filling volume (incl.
CellTram® Air · Oil · vario — Operating manual 8 8 8.1 Ordering information Ordering information CellTram Order no. (international) Description 5176 000.017 CellTram Air 5176 000.025 CellTram Oil 5176 000.033 CellTram vario 8.2 Accessories Order no. (international) Description 5176 114.004 Pressure tube 1m 5176 190.002 Universal capillary holder For holding Femtotips, Femtotips II or grip heads Including grip head 0, adapter for Femtotips and spare O-rings 5176 210.
CellTram® Air · Oil · vario — Operating manual 8.3 Capillaries Various capillaries for sperm injection and the containment of cells are available. VacuTips have been optimized for holding suspension cells (e.g., oocytes or blastocysts). TransferTips have been especially developed for transferring sperm in ICSI applications. Microcapillary VacuTip Microcapillary for holding suspension cells.
CellTram® Air · Oil · vario — Operating manual 9 9 9.1 Transport, storage and disposal Transport, storage and disposal Storage Air temperature Relative humidity Atmospheric pressure In transport packaging -25 °C – 55 °C 10 % – 95 % 70 kPa – 106 kPa Without transport packaging 10 % – 95 % 70 kPa – 106 kPa 9.
Your local distributor: www.eppendorf.com/worldwide Eppendorf AG · 22331 Hamburg · Germany · Tel: +49 40 53801-0 · Fax: +49 40 538 01-556 · E-mail: eppendorf@eppendorf.com Eppendorf North America, Inc. · 102 Motor Parkway · Hauppauge, N.Y. 11788-5178 · USA Tel: +1 516 334 7500 · Toll free phone: +1 800-645-3050 · Fax: +1 516 334 7506 · E-mail: info@eppendorf.com Application Support Europe: Tel: +49 1803 666 789 (Preis je nach Tarif im Ausland; 9 ct/min aus dem dt.
