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Table of contents Centrifuge 5427 R English (EN) Table of contents Operating Centrifuge on see p. Tab. Fig. p. manual 5427 R English Operating (EN) manual 1 Operating instructions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5 1.1 Using this manual . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5 1.2 Danger symbols and danger levels . . . . . . . . . .
Table of contents Centrifuge 5427 R English (EN) 5.5 5.6 5.7 Cooling . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 30 5.5.1 Set the temperature. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 30 5.5.2 Temperature display . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 30 5.5.3 Temperature monitoring . . .
Operating instructions Centrifuge 5427 R English (EN) 1 1.1 Operating instructions Using this manual Read this operating manual completely before using the device for the first time. Please also note the operating instructions for the accessories, if applicable. This operating manual is part of the product. Thus, it must always be easily accessible. Enclose this operating manual when transferring the device to third parties. If this manual is lost, please request another one.
Operating instructions Centrifuge 5427 R English (EN) 1.
Product description Centrifuge 5427 R English (EN) 2 2.1 Product description Main illustration Abb. 2-1: Front and rear view of the Centrifuge 5427 R USB 6 7 1 2 open 3 menu enter short start stop rpm rcf 4 fast temp 5 8 9 10 Fig.
Product description Centrifuge 5427 R English (EN) 2.2 Delivery package Quant Order no. Order no. Description ity (International) (North America) Centrifuge 5427 R 1 See chapter Ordering Information for corresponding device version, equipment and order number Fuse 1 5301 850.249 022654403 4.0 A (230 V), 2 pieces or 5427 850.341 022654381 8.0 AT UL (120 V/100 V), 2 pieces or 5811 352.006 022664107 10.0 AT UL (100 V), 2 pieces Rotor key 1 5416 301.001 022634305 Standard 1 Mains power cable 1 5409 850.
Product description Centrifuge 5427 R English (EN) 2.4 Rotors Eppendorf centrifuges may only be operated with rotors that are intended for use with the corresponding centrifuge. Only use rotors which are marked with the name of the centrifuge (e.g., 5427 R). 2.4.1 Rotor FA-45-12-17 Rotor FA-45-12-17 Aerosol-tight fixed-angle rotor for 12 reaction tubes Vessels Vessel Capacity Max. g-force: 20 598 × g Max. speed: 14 000 rpm Max. load (adapter, tube and contents): 12 × 9.
Product description Centrifuge 5427 R English (EN) 2.4.2 Rotor FA-45-24-11 Rotor FA-45-24-11 Aerosol-tight fixed-angle rotor for 24 tubes Vessels Vessel Capacity Max. g-force: 25 001 × g Max. speed: 16 220 rpm Max. load (adapter, tube and contents): 24 × 3.75 g Adapters Adapter base Max. g-force: Order no. (international) Tube diameter Max. speed Vessels per adapter/rotor Vessel Centrifugation radius – 1.5 mL/2.0 mL – 25 001 × g Ø 11 mm 16 220 rpm -/24 8.
Product description Centrifuge 5427 R English (EN) 2.4.3 Rotor FA-45-24-11-Kit Rotor FA-45-24-11-Kit Aerosol-tight fixed-angle rotor for 24 tubes Vessels Vessel Capacity Max. g-force: 19 090 × g Max. speed: 13 200 rpm Max. load (adapter, tube and contents): 24 × 3.75 g Adapters Adapter base Max. g-force: Order no. (international) Tube diameter Max. speed Vessels per adapter/rotor Vessel Centrifugation radius – 1.5 mL/2.0 mL – 19 090 × g Ø 11 mm 13 200 rpm -/24 9.
Product description Centrifuge 5427 R English (EN) 2.4.4 Rotors FA-45-30-11 and F-45-30-11 Rotor FA-45-30-11 Aerosol-tight fixed-angle rotor for 30 tubes Rotor F-45-30-11 Fixed-angle rotor for 30 tubes Vessels Vessel Capacity Max. g-force: 20 817 × g Max. speed: 14 000 rpm Max. load (adapter, tube and contents): 30 × 3.75 g Adapters Adapter base Max. g-force: Order no. (international) Tube diameter Max. speed Vessels per adapter/rotor Vessel Centrifugation radius – 1.5 mL/2.
Product description Centrifuge 5427 R English (EN) 2.4.5 Rotors FA-45-48-11 and F-45-48-11 FA-45-48-11 rotor Aerosol-tight fixed-angle rotor for 48 tubes Rotor F-45-48-11 Fixed-angle rotor for 48 tubes Vessels Vessel Capacity Max. g-force: 18 213 × g (outer ring) 16 049 × g (inner ring) Max. speed: 12 700 rpm Max. load (adapter, tube and contents): 48 × 3.75 g Adapters Adapter base Max. g-force: Outer ring Inner ring Order no. (international) Tube diameter Max.
Product description Centrifuge 5427 R English (EN) 2.4.6 Rotor F-45-48-5-PCR Rotor F-45-48-5-PCR Fixed-angle rotor for 48 PCR tubes Tubes Tube Max. g-force: 11 710 × g Max. speed: 10 500 rpm Max. load (tube and contents): 48 × 0.43 g Adapter Capacity Adapter base Max. g-force: Tube diameter Max. speed Tubes per adapter/ rotor Centrifugation radius – 0.2 mL conical 11 710 × g Ø 6 mm 10 500 rpm –/48 PCR strips 9.5 cm – 0.2 mL conical 11 710 × g Ø 6 mm 10 500 rpm –/6 × 8 2.
Safety Centrifuge 5427 R English (EN) 3 3.1 Safety Intended use The Centrifuge 5427 R is intended exclusively for indoor use and for separating aqueous solutions and suspensions of various densities in approved test tubes. 3.2 User profile This device may only be operated by trained specialist staff. They must have carefully read the operating manual and be familiar with the function of the device. 3.3 Application limits 3.3.
Safety Centrifuge 5427 R English (EN) Beginning with the first commissioning, the following rotors including the corresponding buckets, carriers and rotor lids have a maximum service life of 7 years or of the number of cycles specified in the table (depending on what occurs first).
Safety Centrifuge 5427 R English (EN) 3.5 Warnings for intended use Read the operating manual and observe the following general safety instructions before using the Centrifuge 5427 R. 3.5.1 Personal injury or damage to the equipment WARNING! Electric shock due to damage to device or mains cable. Only switch on the device if the device and mains cable are undamaged. Only use devices that have been properly installed or repaired.
Safety Centrifuge 5427 R English (EN) CAUTION! Poor safety due to incorrect accessories and spare parts. The use of accessories and spare parts other than those recommended by Eppendorf may impair the safety, functioning and precision of the device. Eppendorf cannot be held liable or accept any liability for damage resulting from the use of incorrect or non-recommended accessories and spare parts, or from the improper use of such equipment.
Safety Centrifuge 5427 R English (EN) CAUTION! Risk of injury from overloaded rotor. The Centrifuge 5427 R is designed for the centrifugation of material with a max. density of 1.2 g/mL at maximum speed and filling volume and/or load. Do not exceed the maximum load of the rotor. NOTICE! Damage to rotors from aggressive chemicals. Rotors are high-quality components which withstand extreme stresses. This stability can be impaired by aggressive chemicals.
Safety Centrifuge 5427 R English (EN) 3.5.5 Aerosol-tight centrifugation WARNING! Risk to health due to limited aerosol tightness with incorrect rotor/rotor lid combination. Aerosol-tight centrifugation is guaranteed only if the rotors and rotor lids intended for this purpose are used. The designation of aerosol-tight fixed-angle rotors always starts with FA.The aerosol-tight rotors and rotor lids of this centrifuge are additionally marked with a red ring on the rotor and a red rotor lid screw.
Installation Centrifuge 5427 R English (EN) 4 4.1 Installation Selecting the location NOTICE! If an error occurs, the objects in the immediate proximity of the device will be damaged. In accordance with recommendations in EN 61010-2-020, leave a safety clearance of 30 cm around the device during operation. Please remove all materials and objects from this area. NOTICE! Damage from overheating. Do not install the device near heat sources (e.g. heating, drying cabinet).
Installation Centrifuge 5427 R English (EN) 4.3 Installing the instrument Prerequisites The device is on a suitable lab bench. NOTICE! Damage to electronic components due to condensation. Condensate can form in the device after it has been moved from a cool environment to a warmer environment. After installing the device, wait for at least 4 h. Only then connect the device to the mains power supply. NOTICE! Centrifuge 5427 R: compressor damage after improper transport.
Operation Centrifuge 5427 R English (EN) 5 5.1 Operation Overview of operating controls Before using the Centrifuge 5427 R, familiarize yourself with the display and operating controls. Abb. 5-1: Operator panel of the Centrifuge 5427 R 1 10 Fig. 5-1: 2 10 4 3 time temp speed 9 8 7 5 6 Operator panel of the Centrifuge 5427 R 1 Call and select the menu parameters (see Menu navigation on p.
Operation Centrifuge 5427 R English (EN) Abb. 5-2: Display of the Centrifuge 5427 R. 1 2 3 4 5 6 9 Fig. 5-2: 7 8 Display of the Centrifuge 5427 R. 1 Centrifugation time 6 g-force (rcf) or speed (rpm) (see Rotors on p. 9) 2 Key lock Key lock. Centrifugation parameters cannot be 7 Status of centrifuge accidentally changed. : Centrifuge lid unlocked. No key lock. Centrifugation parameters can : Centrifuge lid locked. be changed. (Flashing): Centrifuging in progress.
Operation Centrifuge 5427 R English (EN) 5.2 Menu navigation Proceed as follows to change settings in the device menu: 1. Open the menu. 2. Select the desired menu item. 3. Confirm your selection. 4. Select the setting of the corresponding parameters. 5. Confirm the changed setting. The BACK menu item belonging to the first menu level appears. 6. Exit the menu. To exit the second menu level without changing a parameter, select the BACK menu item and confirm with menu/enter. 5.3 Menu Tab.
Operation Centrifuge 5427 R English (EN) Menu level 1 (M 1) Menu level 2 (M 2) SHORT Set the speed of the short spin centrifugation. No SOFT function with short spin centrifugation. MAX Short spin centrifugation at the maximum speed of the inserted rotor Set Short spin centrifugation with set speed (g-force (rcf) or speed (rpm)). Temp Set the time limit for continuous cooling ("ECO shut-off") (see p. 30). 8h Default setting: Continuous cooling ends after 8 h.
Operation Centrifuge 5427 R English (EN) 5.4 Preparing for centrifugation 5.4.1 Switching on the centrifuge Switch the centrifuge on using the mains/power switch. The centrifuge lid opens automatically after switching on using the mains/power switch. The parameter settings of the last run are displayed. 5.4.2 Inserting the rotor 1. Fit the rotor vertically on the motor shaft. 2. Insert the supplied rotor key into the rotor nut. 3. Turn rotor key clockwise until the rotor nut is firmly tightened.
Operation Centrifuge 5427 R English (EN) 5.4.5 Loading the rotor 5.4.5.1 Loading a fixed-angle rotor CAUTION! Risk of injury due to asymmetric loading of a rotor. Load rotors symmetrically with identical tubes. Only load adapters with suitable tubes. Always use the same type of tubes (weight, material/density and volume). Check symmetric loading by balancing the adapters and tubes used with scales. CAUTION! Risk from damaged or overloaded tubes.
Operation Centrifuge 5427 R English (EN) Spin columns For centrifuging spin columns in the rotor FA-45-24-11-Kit, you can leave the tube lids open. However, this can only be done using the tubes provided by kit manufacturers for this purpose. For reliable centrifugation, you must lean the open tube lids against the edge of the rotor. Ensure that the lids do not protrude past the edge of the rotor in the process, then attach the matching rotor lid. 5.4.5.
Operation Centrifuge 5427 R English (EN) 5.4.6 Closing the rotor lid 1. Only QuickLock rotor lid: check that the external sealing ring correctly fits in the groove. 2. Vertically fit the rotor lid on the rotor. 3. Turn the rotor lid screw clockwise to seal the rotor. Only QuickLock rotor lid: Turn the rotor lid screw clockwise as far as it will go, until an audible "click" can be heard. The rotor is not properly closed until an audible click is heard! 5.4.
Operation Centrifuge 5427 R English (EN) 5.5.3 Temperature monitoring After the set temperature has been reached, the centrifuge reacts to temperature deviations during centrifugation as follows: Deviation from target temperature Action ±3 °C Temperature display flashes. ±5 °C Display shows Err 18. Centrifugation is stopped automatically. 5.5.4 Start FastTemp With the FastTemp function, you can immediately start a temperature run without samples, at rotor-specific or temperature-specific speeds.
Operation Centrifuge 5427 R English (EN) 5.5.5 Continuous cooling Continuous cooling Continuous cooling maintains the rotor chamber at the set temperature if the rotor stops. • During continuous cooling the display shows the set temperature. • To prevent the rotor chamber from freezing or condensation from forming, the temperature does not go below 4 °C , irrespective of the set temperature. • If the rotor stops, temperature control is slower than during centrifugation or a temperature control run.
Operation Centrifuge 5427 R English (EN) 5.6 Centrifuging CAUTION! Risk from incorrectly-loaded rotors and damaged/overloaded tubes! Before commencing centrifugation, follow the safety instructions relating to risks from asymmetrically loaded and/or overloaded rotors and from overloaded, damaged and/or open tubes (see Warnings for intended use on p. 17). WARNING! Risk of injury from improperly attached rotors and rotor lids. Only centrifuge with rotor and rotor lid firmly tightened.
Operation Centrifuge 5427 R English (EN) 5.6.2 Centrifuging in continuous operation Perform the following steps in the sequence described. 1. Use time to set the continuous run. The continuous run function can be set above 9:59 h or below 10 s. The timer shows oo to indicate continuous operation. 2. Use temp to set the temperature. 3. With speed, set the g-force (rcf) or speed (rpm). 4. Press the start/stop key to start the centrifugation. The symbol blinks in the display when the rotor is running.
Operation Centrifuge 5427 R English (EN) 5.6.4 Setting the centrifugation radius If an adapter for tubes is used in a rotor, this changes the centrifugation radius. In the RAD menu item, the parameter for the tube/adapter combination has to be set. Prerequisites • Rotor is inserted. • The centrifuge has detected the rotor (see Automatic rotor detection on p. 27). 1. Press the menu/enter key. 2. Select the RAD menu item using the arrow key. Confirm with the menu/enter key.
Operation Centrifuge 5427 R English (EN)
Maintenance Centrifuge 5427 R English (EN) 6 6.1 Maintenance Prepare cleaning/disinfection Clean all accessible surfaces of the device and the accessories at least weekly and when contaminated. Clean the rotor regularly. This way the rotor is protected and the durability is prolonged. Furthermore, observe the notes on decontamination (see Decontamination before shipment on p. 40) when the device is sent to the authorized Technical Service for repairs.
Maintenance Centrifuge 5427 R English (EN) Autoclaving All rotors, rotor lids and adapters can be autoclaved (121 °C, 20 min). Aerosol tightness Check that the seals are intact before use. Only QuickLock rotor lid: Replace the sealing ring in the lid groove when it becomes worn. The sealing rings require regular care to protect the rotors.
Maintenance Centrifuge 5427 R English (EN) 4. Rinse the rotors and accessories thoroughly with distilled water. Rinse the rotor bores of fixed-angle rotors particularly thoroughly. Do not immerse the rotor in liquid as liquid can get trapped inside the cavities. 5. Place rotors and accessories on a cloth to dry. Place fixed-angle rotors with the rotor bores facing downwards to allow the bores to also dry. 6. Correctly reinsert the rotor lid sealing ring in the clean and dry groove. 7.
Maintenance Centrifuge 5427 R English (EN) How to proceed in case of glass breakage 1. Remove all splinters and glass powder from the rotor chamber and accessories. 2. Thoroughly clean the rotor and rotor chamber. Thoroughly clean the bores of the fixed-angle rotors, in particular. 3. If required, replace the adapters to prevent any further damage. 4. Regularly check the rotor bores for deposits and damage. 6.5 Replacing fuses DANGER! Electric shock.
Troubleshooting Centrifuge 5427 R English (EN) 7 Troubleshooting If you cannot remedy an error with the recommended measures, please contact your local Eppendorf partner. The contact address can be found online at: www.eppendorf.com. 7.1 General errors Symptom/message Cause Remedy No display. No mains power connection. Check the mains power connection. No display. Power failure. Check the mains fuse of the laboratory. Lid of the device cannot be opened. Rotor is still running.
Troubleshooting Centrifuge 5427 R English (EN) 7.2 Error messages Proceed as follows if the following error messages appear: 1. Remove fault (see remedy). 2. If necessary, repeat centrifugation. Symptom/message Cause Remedy ERR 1 Check rotor. If this error message appears again, Rotor not detected. test with a different rotor. Err 2 Electronics fault. Switch centrifuge off and back on again after > 20 s. NO RPM (ERR 3) Error in speed measuring system.
Troubleshooting Centrifuge 5427 R English (EN) Symptom/message Cause Remedy Err 22 Switch centrifuge off and back on Electronics fault. again after > 20 s. NO E-FAN (ERR 23) Electronic fan is faulty. Switch centrifuge off and back on again after > 20 s. Err 24 Failure at the compressor. Allow the centrifuge to cool down and repeat the run. INT (ERR 25) Power failure during a run. Check the mains connection. Err 28 Error during speed check.
Troubleshooting Centrifuge 5427 R English (EN) 7.3 Emergency release If the centrifuge lid cannot be opened, you can activate the emergency release manually. WARNING! Risk of injury from rotating rotor. If the emergency release of the lid is operated, the rotor may continue rotating for several minutes. Wait until the rotor stop before operating the emergency release. Check the monitoring glass in the centrifuge lid.
Transport, storage and disposal Centrifuge 5427 R English (EN) 8 8.1 Transport, storage and disposal Transport CAUTION! Risk of injury due to lifting and carrying heavy loads The device is heavy. Lifting and carrying the device can lead to back injuries. Transport and lift the device with an adequate number of helpers only. Use a transport aid to transport the device. Remove the rotor from the centrifuge before transport.
Transport, storage and disposal Centrifuge 5427 R English (EN)
Technical data Centrifuge 5427 R English (EN) 9 9.1 Technical data Power supply Centrifuge 5427 R Mains connection 230 V, 50 Hz – 60 Hz 120 V, 50 Hz – 60 Hz 100 V, 50 Hz – 60 Hz Current consumption 2.4 A (230 V ) 4.6 A (120 V ) 5.
Technical data Centrifuge 5427 R English (EN) 9.4 Application parameters Run time 10 s – 9:59 h, infinite (∞), • 10 s – 2 min: can be set in increments of 10 s • 2 min – 10 min: can be set in increments of 30 s • 10 min – 9:59 h: can be set in increments of 1 min Temperature -11 °C – 40 °C Relative centrifugal force 1 × g – 25 001 × g can be set in increments of 50 × g Speed 100 rpm – 16 220 rpm can be set in increments of 50 rpm Maximum load 48 tube with 2.
Technical data Centrifuge 5427 R English (EN) 9.
Technical data Centrifuge 5427 R English (EN)
Ordering Information Centrifuge 5427 R English (EN) 10 10.1 Ordering Information Centrifuge 5427 R Order no. (International) 5409 000.012 5409 000.136 5409 000.039 Order no. (North America) 5409000136 10.2 Accessories 10.2.1 Rotors and rotor lids Order no. (International) Order no. (North America) 5409 702.009 5409702009 5409 703.005 5409703005 5409 717.006 5409717006 5409 706.004 5409706004 5409 707.000 5409707000 5820 762.004 5820762004 5409 708.007 5409708007 5409 709.
Ordering Information Centrifuge 5427 R English (EN) Order no. (International) Order no. (North America) 5409 713.000 5409713000 5409 704.001 5409704001 5409 705.008 5409705008 5820 767.006 5820767006 5409 700.006 5409700006 5409 701.002 5409701002 5409 716.000 5409716000 5409 714.007 5409714007 5409 715.003 5409715003 5409 720.007 5409720007 5409 721.003 5409721003 5409 719.
Ordering Information Centrifuge 5427 R English (EN) 10.2.3 Other accessories Order no. (International) Order no. (North America) 5416 301.001 5409 850.083 022634305 10.3 Description Rotor key Standard Tray for condensation water Safety Order no. (International) Order no. (North America) 5301 850.249 5427 850.341 5811 352.006 022654403 022654381 022664107 Description Fuse 4.0 A (230 V), 2 pieces 8.0 AT UL (120 V/100 V), 2 pieces 10.
Ordering Information Centrifuge 5427 R English (EN)
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