Repeater nual ® (X)stream gEN) manual Register your instrument! www.eppendorf.
Copyright© 2014 Eppendorf AG, Hamburg. All rights reserved, including graphics and images. No part of this publication may be reproduced without the prior permission of the copyright owner. Eppendorf® and the Eppendorf logo are registered trademarks of Eppendorf AG, Hamburg, Germany. Combitips®, Combitips advanced®, Combitips plus®, Biopur®, Multipette stream®, Multipette Xstream®, PhysioCare Concept®, Repeater® are registered trademarks of Eppendorf AG, Hamburg, Germany.
Table of contents Multipette®/Repeater ® (X)stream 3 English (EN) Table of contents 1 Operating instructions. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1.1 Using this manual . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1.2 Danger symbols and danger levels . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1.2.1 Danger symbols . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Table of contents 4 Multipette®/Repeater ® (X)stream English (EN) 5.8 5.9 5.10 5.11 Pipetting . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Titration. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Stopping or canceling pipetting operations . . . . . . . . . . . . . . . . . . . . . . . . . Adjusting the device settings . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Operating instructions Multipette®/Repeater ® (X)stream 5 English (EN) 1 1.1 Operating instructions Using this manual Read this operating manual completely before using the device for the first time. Please also note the operating instructions for the accessories, if applicable. The instructions for use for the Combitips advanced can be found on our webpage www.eppendorf.com/cbt. This operating manual is part of the product. Please keep it in a place that is easily accessible.
Operating instructions 6 Multipette®/Repeater ® (X)stream English (EN) 1.3 Symbols used Depiction Meaning 1. 2. Actions in the specified order Actions without a specified order • List: Step in the figures 1 Direction of movement Text Display or software texts Additional information 1.4 Glossary A Adapter advanced Connecting piece for the dispenser when using Combitips advanced 25 mL and 50 mL B Biopur Eppendorf Biopur® is an Eppendorf AG purity grade for consumables.
Operating instructions Multipette®/Repeater ® (X)stream 7 English (EN) F Free jet dispensing Dispensing the liquid without the Combitip touching the tube inner wall. If a drip forms on the Combitip after free jet dispensing, this drip always belongs to the next dispensing step. We recommend completing a dispensing series using only the free jet method or wall dispensing method. Complete the reverse stroke in accordance with the dispensing series using either the free jet or wall dispensing method.
Operating instructions 8 Multipette®/Repeater ® (X)stream English (EN) V Viscosity Viscosity describes the viscidity of liquids and suspensions. The dynamic or absolute viscosity is indicated in Pa·s or in mPa·s. In older literature, the unit P or cP is used (1 mPa·s corresponds to 1 cP). At room temperature, a 50 % glycerin solution has a viscosity of approx. 6 mPa·s. As the glycerin concentration increases, viscosity increases considerably. Absolutely anhydrous glycerin has a viscosity of approx.
Product description Multipette®/Repeater ® (X)stream 9 English (EN) 2 2.1 Product description Main illustration Abb. 2-1:Front view Asp Dis Ad s select reset Pip Ttr Seq +/– 1 2 3 4 5 6 7 8 9 1 Connector socket For connecting the charging plug of an Eppendorf Multipette stream/Repeater stream power supply device. 2 Hardware reset button Triggering a hardware reset interrupts the power supply (see p. 40). 3 Selection dial For setting the operating mode.
Product description 10 Multipette®/Repeater ® (X)stream English (EN) 1 Viewing window for the infrared interface For Service. Abb. 2-2:Side view 1 am ® Xstre ette Multip 2 3 4 2 Battery compartment lid For covering the battery compartment. The battery compartment contains the serial number of the Multipette stream/Repeater stream. 3 Charging contact and mounting For the battery charging option and for fixing the Multipette stream/Repeater stream in the charging stand (additionally available).
Product description Multipette®/Repeater ® (X)stream 11 English (EN) 2.3 Features In combination with a Combitip, the Multipette stream/Repeater stream is a motor-driven and electronically controlled manual dispenser which operates according to the positive displacement principle. 2.3.
Product description 12 Multipette®/Repeater ® (X)stream English (EN) 2.4 Warranty In the case of warranty claims, please contact your responsible Eppendorf contractual partner. No warranty is provided for any damage due to misuse or if the device has been opened by unauthorized persons. The rechargeable lithium-ion battery and all other wear parts are excluded from the warranty. 2.5 Display The display has 10 brightness levels. The display switches off during usage intervals.
Product description Multipette®/Repeater ® (X)stream 13 English (EN) Abb. 2-3:Display 2 3 100.0 µL 1 Fig. 2-3: 4 • 20 1 Volume Unit in μL or mL. 2 Direction of the next piston movement Aspirate liquid Dispense liquid 3 Key lock 6 5 Display is only displayed if the +/- rocker switch is locked. Can be stored or activated for the device parameters (see p. 34). 4 Battery Battery charging status.
Product description 14 Multipette®/Repeater ® (X)stream English (EN) 2.6.1 Combitips advanced ^ 0.1 mL 0.2 mL 0.5 mL 1 mL 2.5 mL 5 mL 10 mL 25 mL 2.6.2 Volume ranges for Combitips advanced Combitips advanced Volume range Increment 0.1 mL white 1.0 μL – 100 μL 0.1 μL 0.2 mL light blue 2.0 μL – 200 μL 0.2 μL 0.5 mL purple 5.0 μL – 500 μL 0.5 μL 1.0 mL yellow 10 μL – 1000 μL 1.0 μL 2.5 mL green 25 μL – 2500 μL 2.5 μL 5.0 mL blue 50 μL – 5000 μL 5.0 μL 10 mL orange 0.
Product description Multipette®/Repeater ® (X)stream 15 English (EN) 2.7 Materials NOTICE! Aggressive substances may damage dispensers, Combitips and accessories. Check the chemical resistance when using organic solvents or aggressive chemicals. Please note the cleaning instructions.
Safety 16 Multipette®/Repeater ® (X)stream English (EN) 3 3.1 Safety Intended use The Multipette stream/Repeater stream is a lab device and in combination with a Combitip advanced it is intended for dispensing aqueous solutions in the volume range of 1 μL – 50 mL. In vivo applications (applications in or on the human body) are not permitted. The !!! use DEV_MODEL !!!! may only be operated by trained specialists.
Safety Multipette®/Repeater ® (X)stream 17 English (EN) CAUTION! Danger to persons from careless use Never point the opening of a Multipette stream/Repeater stream which is equipped with a Combitip at yourself or other persons. Only initiate dispensing if it is safe to do so. With any dispensing task please ensure that you do not endanger yourself and other persons. Check the set speed before commencing dispensing operations.
Safety 18 Multipette®/Repeater ® (X)stream English (EN) NOTICE! Material damage due to incorrect dispensing. When using the larger Combitips and highly viscous solutions the Multipette may heat up. Only use highly viscous solutions for short dispensing series. NOTICE! Incorrect dispensing results due to evaporation. If dispensing is continued with a Combitip that has already been filled after a waiting time, the dispensing volume in the next dispensing step may be slightly reduced due to evaporation.
Installation Multipette®/Repeater ® (X)stream 19 English (EN) 4 4.1 Installation Mains/power supply device assembly 3 1 Power plug adapters a EU b United Kingdom c USA d Australia 2 Mains/power supply device 3 Charging plug Insert the power plug adapter required for your mains/power supply into the opening of the mains/power supply device. If there is any doubt as to which power plug adapter should be used for the power supply unit, you should consult an electrician. 4.
Installation 20 Multipette®/Repeater ® (X)stream English (EN) 4.3 Charge the rechargeable battery WARNING! Incorrect or damaged mains/power supply devices can lead to severe personal injury or damage to the device. Incorrect or damaged mains/power supply devices may cause electric shocks, cause the device to overheat, catch fire, melt, short circuit or similar damage. Only use the mains/power supply device which is included in the delivery for charging the battery.
Installation Multipette®/Repeater ® (X)stream 21 English (EN) You can also use the additionally available charger stand 4880 or the charger carousel 4880 (see Accessories on p. 45). To charge the Multipette stream/Repeater stream using the charger carousel 4880, you must order a suitable charger shell for the Multipette stream/ Repeater stream in addition. 4.4 Assembling the Combitip advanced and Adapter advanced Combitips advanced with a maximum volume of 0.1 mL – 10 mL can be used immediately.
Installation 22 Multipette®/Repeater ® (X)stream English (EN) 4.5 Inserting the Combitip NOTICE! Device damage due to incorrect handling of the inserted Combitip Insert the Combitip straight into the dispenser from below. Do not rotate the inserted Combitip. Never grip the dispenser using the Combitip. The Combitip is easier to insert if the ejector is held pressed down during insertion. 1 2 1. If the Combitip piston has been pushed out, push it back into the cylinder of the Combitip. 2.
Installation Multipette®/Repeater ® (X)stream 23 English (EN) 4.6 Removing the Combitip CAUTION! Personal injury and damage to the Multipette stream/Repeater stream and to the Combitip by operating the ejector at the wrong time. If you eject the Combitip during dispensing, liquid may escape. If the ejector is operated at the wrong time, only the cylinder of the Combitip is released. The piston can only be released if it is in the basic position.
Operation 24 Multipette®/Repeater ® (X)stream English (EN) 5 5.1 Operation Principle of operation This chapter is provided for information purposes only and is not intended to provide instructions. All operating modes have a comparable procedure. The following steps occur in all operating modes except the aspiration (Asp) operating mode: 1 2 3 1 2 4 3 4 Parameter settings 1. The parameters that are available can vary depending on the operating mode.
Operation Multipette®/Repeater ® (X)stream 25 English (EN) Dispensing steps 3 The liquid dispensing angle should always be as steep as possible. A dispensing angle greater than 45° can result in an incorrect dispensing volume during the final dispensing steps. 4. Dispense liquid. You can change some parameters during dispensing. 5. There are two possible variants for continuing execution: • Renewed aspiration Perform further dispensing process with the previously set parameters.
Operation 26 Multipette®/Repeater ® (X)stream English (EN) If you eject the Combitip before pressing the actuate key, the changes will not be saved. CAUTION! Personal injury and damage to the Multipette stream/Repeater stream and to the Combitip by operating the ejector at the wrong time. If you eject the Combitip during dispensing, liquid may escape. If the ejector is operated at the wrong time, only the cylinder of the Combitip is released. The piston can only be released if it is in the basic position.
Operation Multipette®/Repeater ® (X)stream 27 English (EN) Display Parameter function Speed Set time: 5.4 1.0 s Dis Ads Seq Asp Pip Ttr Dispensing speed Select the highest speed for dispensing small volumes in free jet mode. Select a low speed for viscous solutions. If there is a risk of liquid splashing out of the destination tube during dispensing, reduce the dispensing speed. ● ● ● ● ● ● Interval time Distance of time between the dispensing steps. You can choose times between 0.1 - 10 s.
Operation 28 Multipette®/Repeater ® (X)stream English (EN) Long = aspirate 1.Short = no step 2.Short = empty 7. There are two possible variants for continuing execution: • Renewed aspiration a Immerse the Combitip into the liquid to be dispensed. b Press and hold the actuate key for 2 seconds to aspirate the liquid. • Empty the Combitip fully and remove a Hold the Multipette stream/Repeater stream over a tube to dispense the residual liquid. b Press the actuate key twice to empty the residual liquid.
Operation Multipette®/Repeater ® (X)stream 29 English (EN) After complete dispensing, the display shows: Long = aspirate 1.Short = no step 2.Short = empty 7. There are two possible variants for continuing execution: • Renewed aspiration a Immerse the Combitip into the liquid to be dispensed. b Press and hold the actuate key for 2 seconds to aspirate the liquid. • Completely empty the Combitip and remove a Hold the Multipette stream/Repeater stream over a tube to dispense the residual liquid.
Operation 30 Multipette®/Repeater ® (X)stream English (EN) 3. Press the actuate key to aspirate the liquid. The display will then show: Discard next step 4. Hold the Multipette stream/Repeater stream over a waste container or the supply vessel and press the actuate key. The reverse stroke is performed. This step is not a dispensing step. 5. Hold the Combitip over the first tube into which the liquid is to be dispensed. 6. Press the actuate key to dispense the liquid.
Operation Multipette®/Repeater ® (X)stream 31 English (EN) 2. Press the actuate key. The display shows: Press key for special step 3. Hold the Multipette stream/Repeater stream over a waste container or the supply vessel and press the actuate key to trigger a "special step" without aspiration of liquid. The reverse stroke is performed. This step is not a dispensing step. 4. Immerse the Combitip into the liquid to be aspirated. Press the actuate key to aspirate the liquid.
Operation 32 Multipette®/Repeater ® (X)stream English (EN) 4. Hold the Multipette stream/Repeater stream over a waste container or the supply vessel and press the actuate key. The reverse stroke is performed. This step is not a dispensing step. 5. Hold the Combitip over the target vessel. Press the actuate key to dispense the liquid. After complete dispensing, the display shows: Long = aspirate 1.Short = no step 2.Short = empty 6.
Operation Multipette®/Repeater ® (X)stream 33 English (EN) The display will then show: Discard next step 4. Hold the Multipette Xstream/Repeater Xstream over a waste container or the supply vessel and press the actuate key. The reverse stroke is performed. This step is not a dispensing step. 5. Hold the Combitip over the tube into which the liquid is to be titrated. Start titration 6. Keep the actuate key pressed or press the key step by step to dispense the liquid.
Operation 34 Multipette®/Repeater ® (X)stream English (EN) 5.10 Stopping or canceling pipetting operations In an emergency you can cancel pipetting as follows: During aspiration, press the reset or actuate key. After a canceled aspiration operation the aspirated partial volume can be dispensed. During dispensing, 1. press the reset key. Piston movement is stopped. After a stopped dispensing operation it is not possible to correctly dispense the remaining volume. 2.
Operation Multipette®/Repeater ® (X)stream 35 English (EN) To access the device settings, proceed as follows: 1. If a Combitip is in the Multipette stream/Repeater stream: press ejector. The display then shows: TIP: Insert Combitip 2. Press both rocker switches up at the same time. The following appears briefly in the display: Entry system settings 3. Select the parameter to be changed using the select rocker switch. 4. Change the setting of the selected parameter using the +/- rocker switch. 5.
Troubleshooting 36 Multipette®/Repeater ® (X)stream English (EN) 6 6.1 Troubleshooting Error search Symptom/ message Cause Remedy Display: One segment empty. Battery exhausted. 1. Finish dispensing. 2. Charge battery (see p. 20). Display: One segment empty. Battery exhausted. 1. Finish dispensing. 2. Charge battery (see p. 20). Display: Battery severely discharged. 1. Confirm with the actuate key. 2. Charge battery (see p. 20). 3. Operation can be continued with the power supply connected.
Troubleshooting Multipette®/Repeater ® (X)stream 37 English (EN) Symptom/ message Cause Remedy • No battery in the Multipette stream/Repeater stream. • Battery is fully discharged (battery is also discharged when the device is not used). Insert battery and charge it for Battery is defective. Insert a new battery and charge 2 hours. it for 2 hours. Display switches • Overcurrent protection has off during use been triggered. • Combitip was ejected at the wrong time.
Troubleshooting 38 Multipette®/Repeater ® (X)stream English (EN) Symptom/ message Cause Display flashes: • Combitip pressed against the Do not use force to press the tube wall too strongly during or inserted Combitip against the before dispensing. tube wall. If the cylinder has been released: • Ejector operated accidentally and cylinder of the Combitip 1. Carefully slide the cylinder of released while the Combitip the Combitip upward until it was full. locks into place. 2. Press the reset key.
Troubleshooting Multipette®/Repeater ® (X)stream 39 English (EN) Symptom/ message Cause Remedy 1. Hold the Multipette stream/ • The present residual liquid is The filled Repeater stream over an empty no longer required. Combitip should tube. be emptied or • A different Combitip should be removed. 2. To empty the Combitip, press used. the reset key. 3. Press the ejector to remove the Combitip. 4. Dispose of Combitip. Combitip is dripping. • Combitip is not leak tight.
Troubleshooting 40 Multipette®/Repeater ® (X)stream English (EN) 6.2 Pipette self-test CAUTION! Injury to people and material damage from the release of sample material when triggering the self test. When the self test is triggered, the liquid is dispensed from the Combitip. Hold the Multipette stream/Repeater stream over an empty container while you trigger the self test. The Multipette stream/Repeater stream can be tested as follows: 1.
Maintenance Multipette®/Repeater ® (X)stream 41 English (EN) 7 7.1 Maintenance Cleaning NOTICE! Damage to device from unsuitable cleaning fluids or sharp or pointed objects. Unsuitable cleaning fluids can damage the device. Never use corrosive cleaning fluids, strong solvents or abrasive polishes. Check the compatibility with the materials used. Please note the information on chemical resistance (see CD).
Technical data 42 Multipette®/Repeater ® (X)stream English (EN) 8 8.1 Technical data Errors with the Multipette (X)stream/Repeater (X)stream Combitip advanced Volume range Testing volume Error limits Error Systematic error 0.1 mL white Increment: 0.1 μL 1 μL – 100 μL 0.2 mL light blue Increment: 0.2 μL 2 μL – 200 μL 0.5 mL purple Increment: 0.5 μL 5 μL – 500 μL 1 mL yellow Increment: 1 μL 10 μL – 1000 μL 2.5 mL green Increment: 2.
Technical data Multipette®/Repeater ® (X)stream 43 English (EN) Combitip advanced Volume range Testing volume Error limits Error Systematic error 10 mL orange Increment: 0.01 mL 0.1 mL – 10 mL 25 mL red Increment: 0.025 mL 0.25 mL – 25 mL 50 mL light gray Increment: 0.05 mL 0.5 mL – 50 mL Random error ±% ± mL ±% ± mL 1 mL 0.50 0.005 0.25 0.0025 5 mL 0.40 0.02 0.25 0.0125 10 mL 0.40 0.04 0.15 0.015 2.5 mL 0.30 0.0075 0.35 0.0088 12.5 mL 0.30 0.0375 0.25 0.
Technical data 44 Multipette®/Repeater ® (X)stream English (EN) 8.1.1 Battery Type Rechargeable lithium-ion battery Overload protection in combination with the Multipette stream/Repeater stream Capacity 750 mAh / 3.7 V Charging time Approx. 2 hours Number of dispensings max. 2000 (if the battery is fully charged and with the highest aspiration and dispensing speed) Weight Approx. 32 g 8.1.
Ordering Information Multipette®/Repeater ® (X)stream 45 English (EN) 9 9.1 Ordering Information Multipette stream/Repeater stream Order no. (International) Order no. Description (North America) 4986 000.017 – Multipette stream 4986 000.025 – Multipette Xstream – 022460803 Repeater stream – 022460811 Repeater Xstream Order no. (International) Order no. Description (North America) 4986 900.115 4986900115 Operating Manual Multipette/Repeater (X)stream 4986 900.
Ordering Information 46 Multipette®/Repeater ® (X)stream English (EN) A charger stand or charger carousel can be used for storing the Multipette stream/ Repeater stream and simultaneously charging the rechargeable Li-ion battery. The power supply included in the accessories of the Multipette stream/Repeater stream can be connected to the charger stand. The charger carousel is supplied with a more powerful power supply. The charger carousel 4880 is intended for Xplorer pipettes.
Ordering Information Multipette®/Repeater ® (X)stream 47 English (EN) Order no. (International) 0030 089.413 – 0030 089.626 0030 089.774 0030 089.421 – 0030 089.634 0030 089.782 0030 089.430 – 0030 089.642 0030 089.790 0030 089.448 – 0030 089.650 0030 089.804 0030 089.456 – 0030 089.669 0030 089.812 0030 089.464 – 0030 089.677 0030 089.820 Order no.
Ordering Information 48 Multipette®/Repeater ® (X)stream English (EN) Order no. (International) 0030 089.472 – 0030 089.685 0030 089.839 0030 089.480 – 0030 089.693 0030 089.847 Order no. (International) 0030 089.715 0030 089.723 0030 089.731 0030 089.740 Order no.
Ordering Information Multipette®/Repeater ® (X)stream 49 English (EN) 9.4 Accessories anced Combitips adv Combilong/Combitube The Combilong/Combitube is an aspiration tool for the Combitips advanced. It enables liquids to be directly taken out of all bottles. Order no. (International) Combitips advanced Rack A Combitips advanced Rack is available for storing the Combitips advanced (≤ 10 mL). Order no. Description (North America) – 0030 059.
Transport, storage and disposal 50 Multipette®/Repeater ® (X)stream English (EN) 10 10.1 Transport, storage and disposal Decontamination before shipment If you are shipping the device to the authorized Technical Service for repairs or to your authorized dealer for disposal please note the following: WARNING! Risk to health from contaminated device Please observe the information in the decontamination certificate, which is available on our webpage as a PDF (www.eppendorf.com/decontamination).
Transport, storage and disposal Multipette®/Repeater ® (X)stream 51 English (EN) 10.3 Disposal In case the product is to be disposed of, the relevant legal regulations are to be observed. Information on the disposal of electrical and electronic devices in the European Community: Within the European Community, the disposal of electrical devices is regulated by national regulations based on EU Directive 2002/96/EC pertaining to waste electrical and electronic equipment (WEEE).
Patents 52 Multipette®/Repeater ® (X)stream English (EN) 11 Patents U.S. Patent No. 6,499,365 Multipette stream/Xstream Repeater stream/Xstream U.S. Patent No. 6,778,917 Multipette stream/Xstream Repeater stream/Xstream U.S. Patent No. 7,585,468 Multipette stream/Xstream Repeater stream/Xstream U.S. Patent No. 7,731,908 Multipette stream/Xstream Repeater stream/Xstream U.S. Design Patent 384,163 No. Combitip plus 0.1 mL U.S. Design Patent 384,162 No. Combitip plus 2.5 mL U.S.
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