eference® nual 2 g EN) manual Register your instrument! www.eppendorf.
Copyright© 2013 Eppendorf AG, Hamburg. No part of this publication may be reproduced without the prior permission of the copyright owner. Trademarks Eppendorf® and the Eppendorf logo are registered trademarks of Eppendorf AG, Hamburg, Germany. Combitips®, epT.I.P.S.®, and Reference® 2 are registered trademarks of Eppendorf AG, Hamburg, Germany. Registered trademarks are not marked in all cases with ™ or ® in this manual. Further information can be found on the enclosed CD. 4920 900.
Table of contents Eppendorf Reference® 2 English (EN) Table of contents 1 Operating instructions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5 1.1 Using this manual . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5 1.2 Danger symbols and danger levels . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5 1.2.
Table of contents Eppendorf Reference® 2 English (EN) 6.5 6.6 Adjusting pipettes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 28 6.5.1 General instructions on user and factory adjustment . . . . . . . . . . . . . . . . . . . . . . . . . . 28 6.5.2 Changing the user adjustment . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 30 6.5.3 Changing the factory adjustment . . . . . . . . . . . . . .
Operating instructions Eppendorf Reference® 2 English (EN) 1 1.1 Operating instructions Using this manual Read this operating manual completely before using the device for the first time. Please also note the operating instructions for the accessories, if applicable. This operating manual is part of the product. Thus, it must always be easily accessible. Enclose this operating manual when transferring the device to third parties. 1.
Operating instructions Eppendorf Reference® 2 English (EN)
Product description Eppendorf Reference® 2 English (EN) 2 2.1 Product description Features Reference 2 pipettes are piston-stroke pipettes which operate according to the air-cushion principle. For air cushion pipettes, a suitable pipette tip must be fitted prior to use. The user controls the dispensing, volume setting and ejector using the control button. When the control button is pressed, the piston in the pipette moves in the same direction.
Product description Eppendorf Reference® 2 English (EN) 2.
Product description Eppendorf Reference® 2 English (EN) 2.3 Main illustration 2.3.1 Reference 2 single-channel 1 2 3 Reference 2 4 J AD 5 6 1 Volume display Volume display with four-digit counter for variable pipettes. Decimal places are marked by a hyphen. 2 Color code The color code on the control button and the trays of the matching epT.I.P.S. pipette tips are in the same color. 3 Control button Sets and dispenses the volume, and ejects the pipette tip.
Product description Eppendorf Reference® 2 English (EN) 2.3.2 Reference 2 Multi-channel For an explanation of the upper part of the pipette, refer to the main illustration of the single-channel pipette. 1 Lever Loosens the multi-channel lower part. 2 Housing lid Removable housing lid with retracted ejector rail. Reference 2 10 AD 0000 3 Multi-channel lower part The multi-channel lower part is freely rotatable. The lower part does not detach when it is rotated.
Product description Eppendorf Reference® 2 English (EN) 2.4 Materials NOTICE! Aggressive substances may damage the Reference 2 and accessories. Check the chemical resistance before using organic solvents or aggressive chemicals. Please note the cleaning instructions. Your can find further information on chemical resistance on the included CD or on our webpage www.eppendorf.com.
Product description Eppendorf Reference® 2 English (EN) 2.5 Storing the pipette The pipette can be safely stored in a pipette carousel or a wall mounting device. 2.5.1 Pipette carousel A pipette carousel (4923) is available for storing Reference 2 pipettes. The pipette carousel features 6 holders that are designed for the Reference 2. Each holder features an image of a Reference 2 pipette to ensure that the pipettes are placed in the correct holder. 2.5.
Safety Eppendorf Reference® 2 English (EN) 3 3.1 Safety Intended use The intended use is described in a separate document enclosed in the package. 3.2 Warnings for intended use WARNING! Damages to health due to infectious liquids and pathogenic germs. When handling infectious liquids and pathogenic germs, observe the national regulations, the biological security level of your laboratory, the material safety data sheets, and the manufacturer's application notes. Wear personal protective equipment.
Safety Eppendorf Reference® 2 English (EN) NOTICE! Damage to device from missing pipette tips. Use the Reference 2 with fitted pipette tips only. When using pipette tips without filters, use 2-10 mL pipettes with protective sleeves and an inserted protection filter. NOTICE! Carry-over, contamination and incorrect dispensing results due to the incorrect use of pipette tips. The pipette tips are for single use only. Prolonged use can have a negative impact on dispensing tasks.
Operation Eppendorf Reference® 2 English (EN) 4 4.1 Operation Setting the volume (only Reference 2 variable) To set the volume, hold the volume locking button pressed down - 4.2 0 0 0 0 10-100 µL and turn the control button. We recommend adjusting the volume setting from a higher value to a lower value. If required, turn beyond the required value and then back again. The height of the control button changes as the volume is adjusted. The volume is displayed up to 1000 μL in μL.
Operation Eppendorf Reference® 2 English (EN) 4.3 Deactivating the tip cone spring loading For the following dispensing tasks, it may be helpful to deactivate the spring loading action of the tip cone. • A pipette tip is to be used over a long period of time. • The pipette tip is slightly bent during dispensing. • For pipette tips that are not designed for the tip cone of Eppendorf pipettes.
Troubleshooting Eppendorf Reference® 2 English (EN) 5 5.1 Troubleshooting Error search Symptom/message Cause Remedy Liquid is dripping The tip is loose or the pipette tip is poorly Firmly attach the pipette tips. Use from the tip and/or fitted. epT.I.P.S. pipette tips. the dispensed volume is incorrect. Liquid with high vapor pressure and/or different density. Wet the tip several times and adjust Pipetting was too quick. Move the control button slowly.
Troubleshooting Eppendorf Reference® 2 English (EN) Regularly check the precision and accuracy of the Reference 2 to prevent dispensing errors. You can use the "PICASO" software to determine the maximum permissible systematic and random errors. If the pipette is used at an extremely high altitude, it must be adjusted to the ambient air pressure. A Standard Operating Procedure (SOP) for checking pipettes can be found on the Reference 2 CD and on our webpage www.eppendorf.com.
Maintenance Eppendorf Reference® 2 English (EN) 6 Maintenance NOTICE! Aggressive substances may damage the Reference 2 and accessories. Check the chemical resistance before using organic solvents or aggressive chemicals. Please note the cleaning instructions. Your can find further information on chemical resistance on the included CD or on our webpage www.eppendorf.com. • All single and multi-channel lower parts are wear parts.
Maintenance Eppendorf Reference® 2 English (EN) 6.2 Sterilizing or disinfecting the pipette NOTICE! Damage to device from incorrect handling. Do not use any additional disinfectants, decontamination agents or sodium hypochlorite during autoclaving or UV exposure. When autoclaving, please ensure that the temperature does not exceed 121°C. Before using disinfecting agents or decontaminating agents, test for material compatibility and check the manufacturer's instructions on chemical resistance.
Maintenance Eppendorf Reference® 2 English (EN) 6.2.2 Disinfection Carefully wipe off the outer surfaces with disinfectant, DNA/RNA decontamination agents or 70 % isopropanol. You can wipe the outside of the tip cone and the ejector sleeve using a sodium hypochlorite solution of 4 %. After the exposure time of the sodium hypochlorite solution (15 minutes) has elapsed, thoroughly remove it using demineralized water.
Maintenance Eppendorf Reference® 2 English (EN) 6.4 Disassembling and assembling the pipette lower part 6.4.1 Single-channel lower part up to 1000 μL 6.4.1.1 Removing the lower part Made in Germany Made in Germany Made in Germany PUSH TO RELEASE 1 2 3 4 1. Hold the control button completely pressed down. 2. Remove the ejector sleeve 1 . 3. 2 and 3 : On the lower part, slide the ring marked "PUSH UP TO RELEASE" up by about 5 mm until the lower part comes off. 4.
Maintenance Eppendorf Reference® 2 English (EN) 6.4.1.3 Opening and assembling the lower part (≤ 1000 μL) To avoid confusion of parts, only disassemble and assemble one pipette at a time. Opening the lower part: 1 2 3 4 1. Push the stop pins together slightly on the piston mount (1). 2. Remove the piston mount. 3. Remove the piston (2) and the piston spring (3). The piston is under spring tension. The appearance of pistons and piston springs will vary for the individual volume versions.
Maintenance Eppendorf Reference® 2 English (EN) 6.4.2 Single-channel lower part Applies to the following single-channel pipettes: • 2 mL • 2.5 mL • 5 mL • 10 mL Replace the protection filter in the tip cone each time it comes into contact with liquid. 6.4.2.1 Exchanging the filter 1. Remove the filter sleeve. 2. Use a pipette tip to push out the old filter. 3. Insert a new filter into the filter sleeve.
Maintenance Eppendorf Reference® 2 English (EN) 4. Insert the filter sleeve. Referen Referen 6.4.2.2 Opening and assembling the lower part (2 mL or larger) ADJ ADJ 4 00 0 0000 00 0 0000 1 2 1 2 3 1. 1 : Unscrew the ejector sleeve. 2. 2 : Keep the control button fully pressed and turn the lower part approximately 30° to the right. The lower part is automatically released from the upper part. 3.
Maintenance Eppendorf Reference® 2 English (EN) The lower part consists of the following: 1 2 3 4 5 6 1 2 mL – 10 mL piston mount 4 Piston (with seal) 2 Piston spring 5 Cylinder and tip cone 3 Upper part of the lower part 6 Filter sleeve with protection filter in the tip cone Before assembling the pipette, first install the piston mount, the piston spring, and the piston in the upper part of the lower part. Further assembly is as shown in the figures.
Maintenance Eppendorf Reference® 2 English (EN) 6.4.3.2 Removing and installing channels 1 1. 1 and 2 2 3 4 5 : Position a pipette tip under the piston and carefully remove the piston from the upper rail. 2. Carefully pull the piston out in an upward direction. Do not bend the piston. 3. Hold the tip cone at the lower end and push it upwards slightly 3 . This compresses the spring. 4. 4 : Lightly lift the tip cone and release it from the lower rail. 5.
Maintenance Eppendorf Reference® 2 English (EN) 6.5 Adjusting pipettes NOTICE! Incorrect dispensing volume due to differences in temperature and for special liquids. Temperature differences between the pipette, pipette tips and liquid; or solutions with physical data that is very different from that of water; may result in incorrect dispensing volumes. Avoid temperature differences the between pipette, pipette tip and liquid.
Maintenance Eppendorf Reference® 2 English (EN) The existing dispensing volume must be checked before changing the factory calibration or adjustment. The actual volume can be checked by weighing: The density of distilled water is approx. 0.9982 mg/μL at 20 °C and 0.9965 mg/μL at 27 °C. If the set volume is the same as the actual volume, no correction is necessary.
Maintenance Eppendorf Reference® 2 English (EN) 6.5.2 Changing the user adjustment If the adjustment is changed, the volume changes by a certain value. Strictly speaking, the change only applies to the testing volume. Example: You readjust a 10 – 100 μL pipette with a volume setting of 100 μL by 1 μL (1 μL ≙ 1 %). If the volume setting is 10 μL, the pipette is also adjusted by 1 μL (≙ 10 %). Auxiliary aids from the delivery package • Pin • Red plastic adjustment seal (ADJ) 1.
Maintenance Eppendorf Reference® 2 English (EN) 6.5.3 Changing the factory adjustment Auxiliary aids from the delivery package • Red plastic safety plug • Pin The factory adjustment can be changed using the corresponding accessories. The factory adjustment will be marked by a safety plug. The color of the safety plug indicates the implementing authority: • Gray - Eppendorf AG • Red - User AD J The random and systematic errors recorded on delivery can be found in the Eppendorf Certificate.
Maintenance Eppendorf Reference® 2 English (EN) 6.6 Decontamination before shipment If you wish to return the device to Eppendorf AG or an Eppendorf AG service partner to be checked or repaired, please note the following: CAUTION! Use of a contaminated device may result in personal injuries and damage to the device. Clean and decontaminate the Reference 2 in accordance with the cleaning instructions before shipping or storage.
Technical data Eppendorf Reference® 2 English (EN) 7 7.1 Technical data Reference 2 Single-channel variable Reference 2 single-channel variable Model Test tip epT.I.P.S. color code Volume range Length Testing volume Eppendorf AG error limits Error Systematic error ±% 0.1 μL – 2.5 μL Increment: 0.002 μL 0.5 μL – 10 μL Increment: 0.01 μL 2 μL – 20 μL Increment: 0.02 μL 2 μL – 20 μL Increment: 0.02 μL 10 μL – 100 μL Increment: 0.1 μL 20 μL – 200 μL Increment: 0.2 μL 30 μL – 300 μL Increment: 0.
Technical data Eppendorf Reference® 2 English (EN) Reference 2 single-channel variable Model Test tip epT.I.P.S. color code Volume range Length Testing volume Eppendorf AG error limits Error Systematic error ±% 0.25 mL – 2.5 mL Red Increment: 0.25 mL – 2.5 mL 0.002 mL 115 mm 0.5 mL – 5 mL Increment: 0.005 mL 1 mL – 10 mL Increment: 0.01 mL 7.2 Purple 0.1 mL – 5 mL 120 mm Turquoise 1 mL – 10 mL 165 mm Random error ± mL ±% ± mL 0.25 mL ±4.8 ±0.012 ±1.2 ±0.003 1.25 mL ±0.8 ±0.010 ±0.
Technical data Eppendorf Reference® 2 English (EN) 7.3 Reference 2 fixed Reference 2 fixed volume Model Test tip epT.I.P.S. color code Volume range Length Eppendorf AG error limits Error Systematic error Random error ±% ± µL ±% ± µL ±2.5 ±0.025 ±1.8 ±0.018 ±2.0 ±0.04 ±1.2 ±0.024 Medium gray 0.1 μL – 20 μL 40 mm ±1.2 ±0.06 ±0.6 ±0.03 ±1.0 ±0.1 ±0.5 ±0.05 20 μL Light gray 0.5 μL – 20 μL L 46 mm ±0.8 ±0.16 ±0.3 ±0.06 10 μL Yellow 2 μL – 200 μL 53 mm ±1.2 ±0.12 ±0.6 ±0.
Technical data Eppendorf Reference® 2 English (EN) 7.4 Test conditions Test conditions and test evaluation match in compliance with ISO 8655, Part 6. Fine balance with evaporation protection inspected by the office of weights and measures • Number of determinations:10 • Use of water in accordance with ISO 3696 • Inspection at 20 °C – 27 °C ±0.5 °C • Dispensing on the tube wall 7.
Ordering Information Eppendorf Reference® 2 English (EN) 8 8.1 Ordering Information Single-channel pipettes, variable volume Order no. (International) 4920 000.016 4920 000.024 4920 000.032 4920 000.040 4920 000.059 4920 000.067 4920 000.075 4920 000.083 4920 000.091 4920 000.105 4920 000.113 8.2 Order no.
Ordering Information Eppendorf Reference® 2 English (EN) 8.3 Single-channel lower part for variable volume and fixed volume Order no. (International) 4920 601.001 4920 602.008 4920 603.004 4920 604.000 4920 605.007 4920 606.003 4920 607.000 4920 608.006 4920 609.002 4920 610.000 4920 611.007 8.4 Order no.
Ordering Information Eppendorf Reference® 2 English (EN) 8.5 Multi-channel lower parts 8.5.1 8-channel lower parts Order no. (International) 4922 601.009 4922 603.001 4922 605.004 8.5.2 4922601009 4922603001 4922605004 Description Eppendorf Reference 2 lower part 8-channel; lower part with separate serial number medium gray, 0.5 - 10 μL yellow, 10 - 100 μL orange, 30 - 300 μL 12-channel lower parts Order no. (International) 4922 602.005 4922 604.008 4922 606.000 8.6 Order no.
Ordering Information Eppendorf Reference® 2 English (EN)
Patents Eppendorf Reference® 2 English (EN) 9 Patents U.S. Patent No. 7,434,484 Is used by Reference 2 pipettes with variable volume setting (single-channel and multi-channel) and fixed volume with following nominal volumes: 1 μL, 2 μL, 2.5 μL, 5 μL, 10 μL, 20 μL, 25 μL, 50 μL, 100 μL, 200 μL, 250 μL, 300 μL, 500 μL and 1000 μL U.S. Patent No. 7,673,532 Is used by Reference 2 pipettes with variable volume setting (multi-channel) with following nominal volumes: 10 μL, 100 μL, 300 μL U.S.
Index Eppendorf Reference® 2 English (EN) Index A Assembly 10 μL, 100 μL and 300 μL multi-channel lower part...................................................................27 Single-channel lower part ...............................24 Single-channel lower part up to 1 000 μL .......23 C Cleaning ................................................................19 D Disassembly 10 μL, 100 μL and 300 μL multi-channel lower part...................................................................
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