® Eppendorf Reference Operating Manual
Eppendorf Reference® 1 Fig. / Abb. 1 Reference variabel / Reference adjustable 0.1 – 2.5 μL 10 – 100 μL 20 – 200 μL 100 – 1000 μL 0.
Teil A / Part A / Section A / Parte A / Parte A Operating Manual ............................................................................4 Bedienungsanleitung ......................................................................22 Manuel d'Utilisation ........................................................................40 Manual de Instrucciones ................................................................58 Istruzioni per l'Uso ....................................................................
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Eppendorf Reference® – Part A – Table of contents Operating Manual 1 2 2.1 2.2 3 4 5 5.1 5.2 6 7 7.1 7.2 7.3 7.4 7.5 8 8.1 8.2 8.2.1 8.2.2 8.2.3 9 9.1 9.2 9.3 10 10.1 4 Main Illustration ................................................................................5 Warning signs and hazard icons ....................................................6 Hazard icons ...................................................................................6 Danger levels ...........................................
Eppendorf Reference® – Part A – Chapter 1 1 Main Illustration 1 Control button The control button is for setting the volume, dispensing and ejecting the pipette tip. 2 Volume display Displays the preset volume. 3 Labeling area The provided autoclavable, blank labels can be labeled with a waterproof pen and affixed at this location. 4 Catch Hold down the catch when you set the volume using the control button.
Eppendorf Reference® – Part A – Chapter 2 2 2.1 Warning signs and hazard icons Hazard icons Biological hazard Health hazard General hazard Material damage Notice 2.2 Danger levels The danger level is part of a safety note and defines the potential consequences if the information contained therein is not observed. DANGER Will lead to serious injuries or death. WARNING May lead to serious injuries or death. CAUTION May lead to minor or moderate injuries. NOTICE May lead to material damage.
Eppendorf Reference® – Part A – Chapter 3 3 WARNING WARNING CAUTION CAUTION General safety notes WARNING! Damage to health due to handling infectious liquids and pathogenic germs. Observe the national regulations for handling these substances, the biological security level of your laboratory, the material safety data sheets and the manufacturer's application notes. Wear your personal protective equipment (PPE). Follow the instructions regarding hygiene, cleaning and decontamination.
Eppendorf Reference® – Part A – Chapter 3 and 4 NOTICE NOTICE NOTICE NOTICE 4 NOTICE! Damage to device from missing pipette tips. Only use the Reference with attached pipette tips. NOTICE! Carry-over, contamination and incorrect dispensing results due to the repeated use of pipette tips. The pipette tips are for single use only. Prolonged use can have a negative impact on dispensing tasks. Use the pipette tips only once. Do not use autoclaved ep Dualfilter T.I.P.S. for dispensing.
Eppendorf Reference® – Part A – Chapter 5 5 Technical data 5.1 Reference fix Model / Volume μL 1 2 5 10 10 20 25 50 100 200 250 500 1,000 1,500 2,000 2,500 5.2 Color code epT.I.P.S. light gray light gray light gray light gray yellow yellow yellow yellow yellow blue blue blue blue red red red 0.5– 20 0.5– 20 0.5– 20 0.5– 20 2– 200 2– 200 2– 200 2– 200 2– 200 50–1,000 50–1,000 50–1,000 50–1,000 500–2,500 500–2,500 500–2,500 μL Reference variable Model μL Color code epT.I.P.S. Volume μL μL 0.
Eppendorf Reference® – Part A – Chapter 5 and 6 The technical data is only valid when the quoted eppendorf pipette tips are used. Tests carried out in accordance with ISO 8655 for piston-stroke pipettes with an air cushion using a precision balance with evaporation trap approved by the standardization authorities. Number of determinations: 10; degassed, bidistilled water, 20 °C – 25 °C, constant to ± 0.5 °C; with prewetted pipette tip; dispensing carried out on inner wall of vessel.
Eppendorf Reference® – Part A – Chapter 7 7 Operation The pipette can be individually labelled. The autoclavable blank adhesive label provided can be marked with a permanent marker and fits onto the identification area on the top of the housing. 7.1 Volume setting The volume is adjusted by pressing down the lateral catch and turning the control button at the same time. It is advisable to carry out volume setting from the higher down to the lower value. i.e.
Eppendorf Reference® – Part A – Chapter 7 7.4 Dispensing liquid – Hold the tip at an angle against the inside wall of the tube. – Press down the control button slowly to the first stop (measuring stroke) and wait until the liquid stops flowing. – Press down the control button to the second stop (blow-out) until the tip is completely empty. – Hold down the control button and pull the tip up the inner wall of the tube. – Tip is ejected by pressing the control button to the final stop. 7.
Eppendorf Reference® – Part A – Chapter 8 8 Testing / Alignment The serial number of the pipette is located on its control button. 8.1 Testing Distilled water is dispensed from a pre-wetted tip into a tube and is then weighed. Volumes 0.1 – 10 μL: The test is performed with a highly sensitive analytic balance (resolution balance: 0.001 mg) by releasing the volume.
Eppendorf Reference® – Part A – Chapter 8 If the place where the pipette is used is at extremely high altitude, an adjustment must be made in line with the ambient air pressure. At 1,000 m above sea level, there is a volume error of approx. –0.4 %. Assistance with these questions is contained in eppendorf‘s SOP (Standard Operating Procedure). An SOP can be called up from our home page www.eppendorf.
Eppendorf Reference® – Part A – Chapter 8 e Adjust control button by the volume determined. One revolution of the control button corresponds to the following values for water: Reference fix Vol./revol. 1, 2, 5, 10 μL 10, 20 μL 25, 50 μL 100 μL 200, 250 μL 500, 1000 μL 1500, 2000, 2500 μL approx. 0.5 approx. 1 approx. 2.4 approx. 5 approx. 12 approx. 46 approx. 118 Clockwise rotation: Counterclockwise rotation: f μL μL μL μL μL μL μL decrease in volume. increase in volume.
Eppendorf Reference® – Part A – Chapter 8 d CAL e Pierce the calibration seal attached to the opening for adjustments with Side B of the key provided. This destroys the seal which should be removed. Carefully attach the key to the adjusting sleeve inside. Turn the wrench to adjust the volume display of the pipette (with piston stroke unchanged) to the actual volume (measurement under step c). f Remove the wrench. g Repeat step c).
Eppendorf Reference® – Part A – Chapter 9 9 9.1 Care / Sterilization Care Depending on the frequency of use, all parts of the pipette should be cleaned from time to time in a soap solution or should be carefully wiped clean using isopropanol. They should then be rinsed in distilled water and dried. The seals are maintenance-free and the pistons should be lubricated lightly (using the grease for pipettes provided) when cleaned or replaced.
Eppendorf Reference® – Part A – Chapter 9 9.3 Decontamination prior to dispatch If the Reference pipette is to be checked, repaired or calibrated by Eppendorf AG or another service partner, it must be free of hazardous substances and clean! A form called "Decontamination certificate for return of goods" and general notes about decontamination are available on our home page: www.eppendorf.com A signed decontamination certificate must be enclosed with the pipette when it is returned.
Eppendorf Reference® – Part A – Chapter 10 10 Troubleshooting Error Cause Solution Droplets on the inner wall of the pipette tip. – Uneven wetting of the plastic wall. – A pipette tip with poor wetting properties has been used. – Attach a new pipette tip. Pipette is dripping and/or the volume pipetted is incorrect. – The tip is loose. – Press the tip firmly in place. – A poorly fitting pipette tip – Use an original eppendorf has been used. tip.
Eppendorf Reference® – Part A – Chapter 10 Error Cause Solution Control button jams, moves erratically. – Piston contaminated. – Clean piston and lubricate lightly. – Disassemble pipette. Clean all seals and exchange where necessary (see Part B, Maintenance). – Unscrew pipette at the central junction and ventilate. Clean piston and lubricate lightly. – Seals contaminated. – Penetration of solvent vapors. Pipette – Liquid has penetrated the tip – Unscrew pipette at the blocked, cone and dried.
Eppendorf Reference® – Part A – Chapter 10 10.1 If there is doubt that dispensing data are correct To avoid dispensing errors, the precision and accuracy of the Reference pipettes need to be checked regularly. The PICASO II software program (see ordering information) is available to determine permitted systematic and random measuring deviation (see Chapter 2 "Technical data"). An SOP (Standard Operating Procedure) for checking pipettes can be called up from our home page www.eppendorf.com.
Eppendorf Reference® – Part B – Contents Maintenance Maintenance I II III IV Exchanging the piston ..................................................................95 Exchanging the seals ....................................................................95 Removing the seals .........................................................................96 Inserting the seals ..........................................................................101 Exchanging the filling tube ............................
Eppendorf Reference® – Part B – Maintenance I Exchanging the piston – Unscrew pipette at the central junction. – Press control button and hold down. Hold piston at the top of the piston mounting and pull off. If the piston fits too tightly, the spring at the piston mounting can be pressed down slightly with side B of the wrench and the piston then pulled off. – Attach new piston down to the stop and lubricate lightly.
Eppendorf Reference® – Part B – Maintenance Removing the seals Fig. 2 – 6 on the following pages show you how to remove the seals. The numbers shown are identical with the numbers in the Ordering information (page 94) and the numbers of the parts on page 1 of this manual. 0.1 – 2.5 μL, 0.5 – 10 μL and 2 – 20 μL (Fig. 2) Push the control button all the way down and pull off the ejector sleeve (13). Piston seal The piston seal in the tip cone is exchanged by replacing the entire tip cone (11).
Eppendorf Reference® – Part B – Maintenance 10 – 100 μL ( Fig. 3) Push the control button all the way down and pull off the ejector sleeve (13). Piston seal (4) – Unscrew pipette. – Discard seal (4) with O-ring. – Insert new seal (see "Inserting the seals"). Tighten the tip cone (see IV of this part). The tip cone (11) is also available complete with seal.
Eppendorf Reference® – Part B – Maintenance 50 – 200 μL ( Fig. 4) Push the control button all the way down and pull off the ejector sleeve (13). Piston seal (4) – Unscrew pipette. – Remove piston seal (4) from tip cone (with the piston in the grip of the pipette) and discard. – Place new piston seal onto tip cone. Tighten the tip cone (see IV of this part).
Eppendorf Reference® – Part B – Maintenance 50 – 250 μL ( Fig. 5) Push the control button all the way down and pull off the ejector sleeve (13). Piston seal (4) – Unscrew pipette at the central junction. – Discard piston seal (4). Insert new piston seal into the clamp (3) and place both into the lower part (5). Ejector seal (10) – Unscrew lower part of the pipette. – Discard seal (10) with O-ring. – Insert new seal (see "Inserting the seals"). Tighten the tip cone (see IV of this part).
Eppendorf Reference® – Part B – Maintenance 100 – 1000 and 500 – 2500 μL (Fig. 6) Push the control button all the way down and pull off the ejector sleeve (13). Piston seal (4) – Unscrew pipette at the central junction. – Discard piston seal (4) and replace. Ejector seal (10) – Unscrew lower part of the pipette. – Discard seal (10) with O-ring. – Insert new seal (see "Inserting the seals"). Tighten the tip cone (see IV of this part). The tip cone (11) is also available complete with ejector seal.
Eppendorf Reference® – Part B – Maintenance Inserting the seals $ & % ' Pull new seal off the pin and push screw, spring, seal (with plastic part first) in that order onto side B of the wrench as shown in the figure and screw lightly into tip cone. Do not tighten too much. Assemble pipette again. III Exchanging the filling tube 10 – 100 μL, 50 – 200 μL and 50 – 250 μL (Fig. 3, 4, 5) Filling tube (12) – Unscrew lower part of the pipette.
Eppendorf Reference® – Part B – Ordering information Ordering Information I Reference fixed-volume (Please see page 1 (Fig. 1) of this manual). Only parts with order numbers are available separately.
Eppendorf Reference® – Part B – Ordering information Order number Order number International North America 1 Piston 1 – 10 μL (gray control button) 20 μL 10 – 20 μL (yellow control button) 25 – 50 μL 100 μL, incl. piston seal (4) 75 – 100 μL, incl. piston seal (4) 150 – 200 μL 250 μL 200 – 250 μL, incl. piston seal (4) 300 – 1000 μL, incl. piston seal (4) 500 – 1000 μL, incl. piston seal (4) 500 – 2500 μL, incl.
Eppendorf Reference® – Part B – Ordering information 5 Lower housing 6 Ejector, includes ejector spring (7) 1 – 20 μL, incl. ejector tube 25 – 100 μL, incl. ejector tube 150 – 200 μL 200 – 250 μL 250 μL 300 – 1000 μL 500 – 1000 μL 1500 – 2500 μL Order number Order number International North America not sold separately 4910 839.000 4910 840.008 4910 841.004 4910 842.000 4910 843.
Eppendorf Reference® – Part B – Ordering information Order number Order number International North America 13 Ejector sleeve 1 – 100 μL 150 – 200 μL 200 – 1000 μL 250 – 1000 μL 500 – 2500 μL 4910 845.000 022475827 022475843 4910 846.006 022475860 4910 847.002 022475886 14 Damping tube not sold separately Lower part complete, incl.
Eppendorf Reference® – Part B – Ordering information II Reference adjustable-volume (Please see page 1 (Fig. 1) of this manual). Only parts with order numbers are available separately. Pipettes / spare parts Models Dark gray control button (for 10 μL pipette tips) 0.1 – 2.5 μL Order number Order number International North America 4910 000.085 022470001 Light gray control button (for 20 μL pipette tips) 0.5 – 10 μL 2 – 20 μL 4910 000.018 022470051 4910 000.
Eppendorf Reference® – Part B – Ordering information Order number Order number International North America 4 Piston seal 10 – 100 μL, incl. screw (8), spring (9) 4910 820.007 50 – 200 μL, with 2 O-rings 4910 824.002 50 – 250 μL, incl. screw (8), spring (9), ejec-4910 821.003 tor seal (10) 100 – 1000 μL, incl. screw (8), spring (9), 4910 822.000 ejector seal (10) 500 – 2500 μL, incl. screw (8), spring (9), 4910 823.
Eppendorf Reference® – Part B – Ordering information Order number Order number International North America 12 Filling tube, (5 pieces, 1 wire punch) 10 – 100 μL 4910 837.007 022475746 50 – 200 μL, incl. 2 pieces of (14) 4910 853.002 022475762 50 – 250 μL 4910 838.003 13 Ejector sleeve 0.1 – 2.5 μL 4910 845.000 022475827 0.5 – 10 μL 4910 845.000 022475827 2 – 20 μL 4910 845.000 022475827 10 – 100 μL 4910 845.000 022475827 50 – 200 μL 4910 852.006 022475843 50 – 250 μL 4910 846.006 100 – 1000 μL 4910 846.
Eppendorf Reference® – Part B – Ordering information III Additional accessories Pipette carousel, incl. 6 pipette supports Order number Order number International North America 3115 000.003 022444905 Pipette holder (replacement for stand) 3115 600.019 022260588 Pipette wall mount (with adhesive surface for attachment to lab benches and wall) PICASO II (Pipette Calibration Software) PICASO accessories 3115 000.020 022444913 3113 004.001 see eppendorf catalog Operating manual 4910 900.
Eppendorf Reference® – Part B – Ordering information IV Pipette tips epT.I.P.S. (The packaging units stated represent the minimum ordering quantity). Length Color code Order number Order number International North America Standard, in bags, 2x 500 = 1000 tips 0.1 – 10 μL 34 mm dark gray 0030 000.811 022492004 0.1 – 20 μL 40 mm medium gray 0030 000.838 022492012 0.5 – 20 μL L 46 mm light gray 0030 000.854 022492021 2 – 200 μL 53 mm yellow 0030 000.870 022492039 50 – 1000 μL 71 mm blue 0030 000.
Eppendorf Reference® – Part B – Ordering information Length Color code Order number Order number International North America Box, 1 box plus 96 tips 0.1 – 10 μL 0.1 – 20 μL 0.5 – 20 μL L 2 – 200 μL 50 – 1000 μL 48 tips 500 – 2500 μL 34 mm 40 mm 46 mm 53 mm 71 mm dark gray medium gray light gray yellow blue 115 mm red Racks, plus 10x96=960 tips 0.1 – 10 μL 34 mm 0.1 – 20 μL 40 mm 0.5 – 20 μL L 46 mm 2 – 200 μL 53 mm 50 – 1000 μL 71 mm 480 tips 500 – 2500 μL 115 mm 0030 073.002 0030 073.029 0030 073.
Eppendorf Reference® – Part B – Ordering informations Length Color codeOrder number International Singles (Eppendorf Biopur), colorless, pyrogen-free, DNA-free, RNase-free, ATP-free, individually wrapped, 100 tips 0.1 – 20 μL 40 mm medium 0030 010.019 gray 2 – 200 μL 53 mm yellow 0030 010.035 50 – 1000 μL 71 mm blue 0030 010.051 Order number North America 022491130 022491148 022491156 ep Dualfilter T.I.P.S., PCR-clean, sterile, in racks, 10x 96 = 960 tips 0.1 – 10 μL S 34 mm dark gray 0030 077.
Eppendorf Reference® – Part B – Ordering informations Length Color code Order number Order number International North America Reloads, LoRetention PCR clean, 10 x 96 = 960 tips 0.1 – 10 μL 34 mm dark gray 0030 072.006 022493010 0.5 – 20 μL L 46 mm light gray 0030 072.014 022493012 2 – 200 μL 53 mm yellow 0030 072.022 022493014 50 – 1000 μL 71 mm blue 0030 072.030 022493016 Reloads, LoRetention, autoclavable, 10 x 96 = 960 tips 0.1 – 10 μL 34 mm dark gray 0030 072.049 022493018 0.
Eppendorf Offices AUSTRALIA & NEW ZEALAND Eppendorf South Pacific Pty. Ltd. Phone: +61 2 9889 5000 Fax: +61 2 9889 5111 E-mail: Info@eppendorf.com.au Internet: www.eppendorf.com.au CANADA Eppendorf Canada Ltd. Phone: +1 905 826 5525 Fax: +1 905 826 5424 E-mail: canada@eppendorf.com Internet: www.eppendorfna.com FRANCE Eppendorf France S.A.R.L. Phone: +33 1 30 15 67 40 Fax: +33 1 30 15 67 45 E-mail: eppendorf@eppendorf.fr Internet: www.eppendorf.fr ITALY Eppendorf s.r.l.
eppendorf® is a registered trademark. B 4910 900.159-02/1010 · Printed in Germany · Printed on chlorine-free bleached paper Your local distributor: www.eppendorf.com/worldwide Eppendorf AG · 22331 Hamburg · Germany · Tel: +49 40 538 01-0 · Fax: +49 40 538 01-556 · E-Mail: eppendorf@eppendorf.com Eppendorf North America, Inc. · 102 Motor Parkway, Suite 410 · Hauppauge, NY 11788-5178 · USA Tel: +1 516 334 7500 · Toll free phone: +1 800 645 3050 · Fax: +1 516 334 7506 · E-Mail: info@eppendorf.
