_Research_Titel.fm Seite 1 Donnerstag, 17.
1_Research_Titel.fm Seite 2 Donnerstag, 17. April 2008 12:31 12 Fig./Abb. 1 Research – variable / fix Single-Channel / Einkanal Research variable 0.1 – 2.5 µL 0.
1_Research_Titel.fm Seite 3 Donnerstag, 17.
1_Research_Titel.fm Seite 4 Donnerstag, 17. April 2008 12:31 12 Contents / Inhalt / Sommaire / Indice / Indice Part A / Teil A / Section A / Parte A / Parte A Instruction Manual . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5 Bedienungsanleitung . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20 Mode d’emploi . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
2_ResearchE.fm Seite 5 Dienstag, 3. November 2009 11:49 11 Research – Part A – Contents 1 1.1 1.2 1.3 2 2.1 2.2 2.3 3 4 4.1 4.2 4.3 4.4 4.5 5 5.1 5.2 5.2.1 5.2.2 5.2.3 6 6.1 6.2 6.2.1 6.2.2 6.3 7 Safety . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6 Intended use . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6 Handling . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
2_ResearchE.fm Seite 6 Dienstag, 3. November 2009 11:49 11 Research – Part A – Section 1 1 Safety 1.1 Intended use The Research is a lab device intended for dispensing liquids in the volume range from 0.1 µL to 10 mL, in combination with matching pipette tips. In vivo applications (applications in or on the human body) are not permitted. The Research may only be operated by trained specialist staff.
2_ResearchE.fm Seite 7 Donnerstag, 28. Mai 2009 11:04 11 Research – Part A – Section 2 2 Technical data 2.1 Research, fixed-volume Model/volume Color code epT.I.P.S. µL 10 20 25 50 100 200 250 500 1,000 yellow yellow yellow yellow yellow blue blue blue blue Systematic error µL % µL 2– 200 2– 200 2– 200 2– 200 2– 200 50–1,000 50–1,000 50–1,000 50–1,000 ± 1.2 ± 1.0 ± 1.0 ± 0.7 ± 0.6 ± 0.6 ± 0.6 ± 0.6 ± 0.6 ± 0.12 ± 0.2 ± 0.25 ± 0.35 ± 0.6 ± 1.2 ± 1.5 ±3 ±6 Random error % ± 0.6 ± 0.3 ± 0.3 ± 0.
2_ResearchE.fm Seite 8 Donnerstag, 17. April 2008 12:31 12 Research – Part A – Sections 2 and 3 2.3 Research, multi-channel, adjustable-volume (variable) (8- and 12-channel) Model µL Color code epT.I.P.S. µL Volume µL Systematic error % µL Random error % µL 0.5– 10 light gray 0.5– 20 1 5 10 ± 8.0 ± 4.0 ± 2.0 ± 0.08 ± 0.2 ± 0.2 ± 5.0 ± 2.0 ± 1.0 ± 0.05 ± 0.1 ± 0.1 10–100 yellow 2–200 10 50 100 ± 3.0 ± 1.0 ± 0.8 ± 0.3 ± 0.5 ± 0.8 ± 2.0 ± 0.8 ± 0.3 ± 0.2 ± 0.4 ± 0.
2_ResearchE.fm Seite 9 Donnerstag, 17. April 2008 12:31 12 Research – Part A – Section 3 It is possible to choose between a lower part for eight channels and for twelve channels. Both cover the same volume range. Fig. 2 Research (single-channel) and multi-channel lower part 1 2 3 4 CAL 1 Control button First stop (measuring stroke); the aspirated volume is dispensed. Second stop (blow-out); the liquid remaining in the tip is blown out.
2_ResearchE.fm Seite 10 Donnerstag, 17. April 2008 12:31 12 Research – Part A – Section 4 4 Operation 4.1 Volume setting (not valid for fixed-volume model) The volume can be changed continuously by rotating the setting ring (Fig. 2-2). The figures in the digital display are read from top to bottom. It is advisable to carry out volume setting from the higher down to the lower value. i.e. first go above the desired volume and then return to the lower value. 4.
2_ResearchE.fm Seite 11 Donnerstag, 17. April 2008 12:31 12 Research – Part A – Section 4 Note: For Research, adjustable-volume (variable), 500–5 000 µL and 1 – 10 mL: if the tip is removed from the liquid too quickly, coaxial forces may push liquid out of the tip. This may result in the pipetted volume being too low. 4.4 Dispensing liquid – Hold the tip(s) at an angle against the inside wall of the tube/well of the microtiter plate. – Press down the control button (Fig.
2_ResearchE.fm Seite 12 Donnerstag, 17. April 2008 12:31 12 Research – Part A – Sections 5 5 Testing / alignment The serial number is on the setting ring on the control button. 5.1 Testing Research, single-channel Distilled water is dispensed from a pre-wetted tip into a tube and is then weighed. Volumes 0.1 – 10 µL: The test is performed with a highly sensitive analytic balance (resolution balance: 0.001 mg) by releasing the volume.
2_ResearchE.fm Seite 13 Donnerstag, 17.
2_ResearchE.fm Seite 14 Donnerstag, 17. April 2008 12:31 12 Research – Part A – Section 5 d To align to the volume displayed, side D (or side B with multi-channel pipettes) of the wrench is inserted horizontally through the calibration seal into the alignment opening (Fig. 2-4) in the pipette grip. (When doing so, hold the pipette vertically.) The wrench is then rotated into a vertical position. The calibration seal is thereby destroyed and removed.
2_ResearchE.fm Seite 15 Donnerstag, 17. April 2008 12:31 12 Research – Part A – Sections 5 and 6 Since this adjustment affects the entire measuring rang, it is imperative to check the other volumes of this pipette specified in the technical data. Next, close the adjustment opening with one of the supplied calibration seals. 5.2.
2_ResearchE.fm Seite 16 Donnerstag, 17. April 2008 12:31 12 Research – Part A – Section 6 In order to ensure the consistent quality of the pipettes, we recommend using the corresponding parts of the same generation (PhysioCare Concept + PhysioCare Concept, Standard + Standard*). * please review old operating instructions Visual differentiating features of PhysioCare Concept pipettes: Single-channel Multi-channel Black piston holder Black tip holder 6.
2_ResearchE.fm Seite 17 Donnerstag, 17. April 2008 12:31 12 Research – Part A – Section 6 Variant 1 – 10 mL (Fig. 1-14) Pull filter down out of the nose cone. The filter swells up slightly during autoclaving. Compress gently when reinserting. This does not impair function. Only autoclave filter 1x. Unscrew the complete lower part from the grip by turning to the left, not turning the ejection sleeve (Fig. 1-12) at the same time.
2_ResearchE.fm Seite 18 Donnerstag, 17. April 2008 12:31 12 Research – Part A – Section 7 7 Troubleshooting Error Cause Droplets on the – Uneven wetting of the plastic wall. inner wall of the pipette tip. – A pipette tip with poor wetting properties has been used. – The tip is loose. Pipette is dripping and/or – A poorly fitting pipette tip the volume has been used. pipetted is – Liquid with a high vapor incorrect. pressure has been pipetted. For 500–5000 µL/1 –10 mL pipette: – Pipetting was too fast.
2_ResearchE.fm Seite 19 Donnerstag, 17. April 2008 12:31 12 Research – Part A – Section 7 Error Cause Solution – The piston is contaminated. – Clean the piston and The control lubricate lightly (Part B, button jams "Maintenance"). and does not move smoothly. – The seal is contaminated. – Remove the piston from the pipette lower part (Part B, "Maintenance"). Rinse out the lower part with warm water, then rinse with distilled water and allow to dry. Replace the seal if necessary.
7_ResearchTeil2_E.fm Seite 80 Donnerstag, 17. April 2008 12:34 12 Research – Part B – Contents Maintenance I. II. Single-channel model . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 82 A Disassembly and assembly . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 82 B Replacing pistons and seals . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 82 Multi-channel model . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
7_ResearchTeil2_E.fm Seite 81 Donnerstag, 17. April 2008 12:34 12 Research – Part B – Maintenance For information on replacing pistons and seals as well as on disassembling and assembling the different members of the Research family, please see page 3 and page 115 of this manual. Single-channel pipettes B A C All maintenance work is carried out using this wrench. Fig. 4 Opening A: Side B: Side C: Side D: to loosen the pipette lower part from the grip. to replace the seals. to replace the seals.
7_ResearchTeil2_E.fm Seite 82 Donnerstag, 17. April 2008 12:34 12 Research – Part B – Maintenance I. Single-channel model Please see page 3 (Fig. 1) of this manual. A general description of disassembly and assembly for all single-channel models is followed by instructions on how to replace specific parts for special pipette types. A Disassembly and assembly (Fig. 1 / point 1) Disassembly – Hold down the ejection button on the side of the pipette and pull off the ejection sleeve (12) using force.
7_ResearchTeil2_E.fm Seite 83 Donnerstag, 17. April 2008 12:34 12 Research – Part B – Maintenance For fixed- and adjustable-volume (variable) pipettes up to 100 µL (see Fig. 1 / point 3): – Disassemble the pipette. – Using side B of the wrench, undo the screw (6) and tap out the internal spring (7). – Press in side C of the wrench, pull out the seal and O-ring (8) and replace.
7_ResearchTeil2_E.fm Seite 84 Dienstag, 3. November 2009 12:06 12 Research – Part B – Maintenance Fixed- and adjustable-volume (variable) pipettes up to 1000 µL (see Fig. 1 / point 5): – Disassemble the pipette. – Undo the screw (6) using side C of the wrench. – Tap out the spring (7), the press piece (9) and the seal (8). Replace the seal. – Attach the spring to the new seal and then insert both parts, together with the screw, into the pipette lower part and tighten.
7_ResearchTeil2_E.fm Seite 85 Dienstag, 3. November 2009 12:06 12 Research – Part B – Maintenance – Pull the locking mechanism (5a), which is located on the cylinder attachment (5), off upwards. – Unscrew cylinder (11) from the cylinder attachment (5) by turning to the left. – Put the disassembly aid supplied over the cylinder attachment (5) from above and press down, which pushes the piston down at the same time.
7_ResearchTeil2_E.fm Seite 86 Donnerstag, 17. April 2008 12:34 12 Research – Part B – Maintenance II. Multi-channel model A Replacing the O-rings C B A – Hold down the ejection button on the side of the pipette and unscrew the lower part counterclockwise from the grip. – Push opening A of the tool (suitable for the pipette size at hand) from below over the nose cones in such a way as to ensure that the sharp edge in the opening of the tool is overlapping with the O-ring Fig. 6 (see Fig. 6).
7_ResearchTeil2_E.fm Seite 87 Donnerstag, 17. April 2008 12:34 12 Research – Part B – Maintenance – Please see page 115 of this manual. – Pull the housing off the cover plate. – Hold the lower part firmly, as shown in Fig. 7. – Press side B of the tool under the metal clip and slide it upwards. Lever the clip off the safety hook. – Turn the lower part until the second clip is facing forwards. When doing so, grip the tip holder with your thumb (the tip holder is subject to spring tension).
7_ResearchTeil2_E.fm Seite 88 Donnerstag, 17. April 2008 12:34 12 Research – Part B – Maintenance 30 – 300 µL – Proceed as with the other sizes, The press piece is discarded. – Using side C of the tool, pull out the spring, spring plate and seal (Fig. 11a) and replace the defective seal. Assembly 0.5 – 10 µL and 10 – 100 µL – Load side C of the tool with the press piece, then the spring, then the spring plate and then the seal. Re-insert side C into the tip holder and press it in (Fig. 11b).
7_ResearchTeil2_E.fm Seite 89 Donnerstag, 28. Mai 2009 11:10 11 Research – Part B – Ordering information Please see page 3 (Fig. 1) of this manual. In order to ensure the consistent quality of the pipettes, we recommend using the corresponding parts of the same generation (PhysioCare Concept + PhysioCare Concept, Standard + Standard*). * please review old operating instructions I.
7_ResearchTeil2_E.fm Seite 90 Donnerstag, 28. Mai 2009 11:10 11 Research – Part B – Ordering information Order number International Order number North America 12 Ejection sleeve 10 – 100 µL 200 – 1000 µL 3110 861.000 3110 865.006 022476491 022476521 13 Filling tube (5 pcs., 1 wire punch) 3110 872.002 022476556 3111 501.016 022476581 Order number International Order number North America 3111 000.114 3111 000.122 3111 000.130 3111 000.149 3111 000.157 3111 000.165 3111 000.173 3111 000.
7_ResearchTeil2_E.fm Seite 91 Dienstag, 3. November 2009 11:56 11 Research – Part B – Ordering information Order number International 5 Cylinder attachment 500 –5000 µL 3110 836.006 1 – 10 mL 3111 602.009 5a Locking mechanism (for 10 mL only) 3111 604.001 6 Screw not sold separately 7 Spring not sold separately 8 Seal 10 – 100 µL, incl. (6),(7) 4910 820.007 20 – 200 µL, incl. (6), (7), (9), centering aid 3110 844.009 100 – 1000 µL, incl. (6),(7) 3110 845.
7_ResearchTeil2_E.fm Seite 92 Dienstag, 3. November 2009 11:56 11 Research – Part B – Ordering information III. Multi-channel pipettes Pipettes/spare parts Order number International 8-channel: 0.5 – 10 µL Light gray control button 3114 000.115 10 – 100 µL Yellow control button 3114 000.131 30 – 300 µL Orange control button 3114 000.158 12-channel: 0.5 – 10 µL Light gray control button 3114 000.123 10 – 100 µL Yellow control button 3114 000.140 30 – 300 µL Orange control button 3114 000.
7_ResearchTeil2_E.fm Seite 93 Dienstag, 3. November 2009 11:56 11 Research – Part B – Ordering information IIIa. Additional accessories Silicone grease (Grease for pipettes), 2 g Silicone grease, 60 g Reagent reservoir "Tip-Tub", Order number International Order number North America 0013 063.010 022348515 022348501 0030 058.607 3111 900.115 3113 004.
7_ResearchTeil2_E.fm Seite 94 Donnerstag, 28. Mai 2009 11:10 11 Research – Part B – Ordering information Length 1 – 10 mL standard (200 tips) 1 – 10 mL long (200 Tips) Set 1 Box incl. 5 x 96 tips 0.1 – 10 µL 0.1 – 20 µL 0.5 – 20 µL L 2 – 200 µL 20 – 300 µL 50 –1000 µL Reloads 10 x 96 = 960 tips 0.1 – 10 µL (in stacks) 0.1 – 20 µL 0.5 – 20 µL L 2 – 200 µL (in stacks) 20 – 300 µL 50 –1000 µL Reloads PCR-clean 10 x 96 = 960 tips 0.1 – 10 µL (in stacks) 0.1 – 20 µL 0.
7_ResearchTeil2_E.fm Seite 95 Donnerstag, 28. Mai 2009 11:10 11 Research – Part B – Ordering information Length Color code Order number International Racks plus 10 x 96 = 960 tips 0.1 – 10 µL 34 mm dark gray* 0.1 – 20 µL 40 mm medium gray** 0.5 – 20 µL L 46 mm light gray 2 – 200 µL 53 mm yellow 20 – 300 µL 55 mm orange 50 –1000 µL 71 mm blue 500 –5000 µL 120 mm purple (120 tips) Racks PCR-clean plus 10 x 96 = 960 tips 0.1 – 10 µL 34 mm dark gray* 0.1 – 20 µL 40 mm medium gray** 0.
7_ResearchTeil2_E.fm Seite 96 Dienstag, 3. November 2009 11:56 11 Research – Part B – Ordering information Length ep Dualfilter T.I.P.S. PCR-clean, sterile in racks 10 x 96 = 960 tips 0.1 – 10 µL S 34 mm 0.1 – 10 µL M 40 mm 0.5 – 20 µL L 46 mm 2 – 20 µL 53 mm 2 – 100 µL 53 mm 2 – 200 µL 55 mm 20 – 300 µL 55 mm 50 –1000 µL 76 mm 100 –5000 µL 120 mm 5 x 24 = 120 tips 1 – 10 mL 243 mm long (100 pcs.) individually blister-packed GELoader® tips (f. 0.
7_ResearchTeil2_E.fm Seite 97 Donnerstag, 28. Mai 2009 11:10 11 Research – Part B – Ordering information Length Color code epT.I.P.S. LoRetention Dualfilter, PCR clean, sterile and pyrogen free, in racks, 10 x 96 = 960 tips 0.1 – 10 µL S 34 mm dark gray 0.5 – 20 µL L 46 mm light gray 2 – 100 µL 53 mm yellow 20 – 300 µL 55 mm orange 50 –1000 µL 76 mm blue Reloads, LoRetention PCR clean, 10 x 96 = 960 tips 0.1 – 10 µL 34 mm dark gray 0.
7_ResearchTeil2_E.fm Seite 98 Donnerstag, 17.
9_ResearchRück.fm Seite 117 Dienstag, 3. November 2009 12:01 12 Eppendorf Offices AUSTRALIA / NEW ZEALAND Eppendorf South Pacific Pty. Ltd. Tel. +61 2 98 89 50 00 Fax +61 2 98 89 51 11 E-Mail: Info@eppendorf.com.au Internet: www.eppendorf.com.au CZECH REP. & SLOVAKIA EPPENDORF Czech & Slovakia s.r.o. Tel. +420 323 605 454 Fax +420 323 605 454 E-Mail: eppendorf@eppendorf.cz Internet: eppendorf.cz Internet: eppendorf.sk AUSTRIA FRANCE Eppendorf Austria GmbH Tel.
9_ResearchRück.fm Seite 118 Dienstag, 3. November 2009 12:01 12 Eppendorf Offices ITALY Eppendorf s.r.l. Tel. +390 2 55 404 1 Fax +390 2 58 013 438 E-Mail: eppendorf@eppendorf.it Internet: www.eppendorf.it JAPAN Eppendorf Co. Ltd. Tel. +81 3 5825 2363 Fax +81 3 5825 2365 E-Mail: info@eppendorf.jp Internet: www.eppendorf.jp NORDIC Eppendorf Nordic Aps Tel. +45 70 22 29 70 Fax +45 45 76 73 70 E-Mail: nordic@eppendorf.dk Internet: www.eppendorf.dk SWITZERLAND Vaudaux-Eppendorf AG Tel.
9_ResearchRück.fm Seite 119 Dienstag, 3. November 2009 12:01 12 Fig./Abb. Research – Maintenance / Wartung Multi-channel / Mehrkanal A B C Fig./Abb. 7 A B C Press piece Niederhalter Presseur Pezzo premente Sujetador Spring Feder Ressort Molla Muelle Spring plate Federteller Plaque pour ressort Piastrina per molla Platillo de muelle Seal Dichtung Joint Guarnizione Junta Fig./Abb. 8 Fig./Abb. 10 B A C B A C a) b) Fig./Abb. 9 Fig./Abb.
In touch with life eppendorf® is a registered trademark. B 3111 900.158-05/1109 · Printed in Germany · Printed on chlorine-free bleached paper 9_ResearchRück.fm Seite 120 Dienstag, 3. November 2009 12:01 12 Your local distributor: www.eppendorf.com/worldwide Eppendorf AG · 22331 Hamburg · Germany · Tel: +49 40 538 01-0 · Fax: +49 40 538 01-556 E-mail: eppendorf@eppendorf.com Eppendorf North America, Inc.
