Manual

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Instructions for use

S-4-72/S-4-104

English (EN)

Instructions for useS-4-72/S-4-104seeon p.Fig.Tab.p.

English (EN)Instructions for use

This document supplements the operation manual Centrifuge 5804/5804 R/5810/5810 R

and does not replace it. Therefore, please also read the operating manual before starting

up the rotors for the first time. You can find the current version on the Internet at

www.eppendorf.com.

1 Product description

The rotor S-4-72 is a swing-bucket rotor with a maximum capacity of 4 × 250 mL for the

Centrifuge 5804/5804 R/5810/5810 R.

The rotor S-4-104 is a swing-bucket rotor with a maximum capacity of 4 × 750 mL for the

Centrifuge 5810/5810 R. This rotor has also been designed for centrifugation of Deepwell

plates, microplates and PCR plates in plate buckets.

2 Safety notes

Rotor S-4-104 only

NOTICE! If handled incorrectly, the rotor can fall over.

The rotor can fall over if the buckets are used as a handle.

 Remove the buckets before inserting and/or removing a swing-bucket rotor.

 Always use both hands to carry the rotor cross.

CAUTION! Risk of injury due to chemically damaged rotor lids or caps.

Transparent rotor lids or caps made from PC, PP or PEI may loose their strength

under the impact of organic solvents (e.g. phenol, chloroform).

 If rotor lids or caps have come into contact with organic solvents, they should

be cleaned immediately.

 Regularly check the rotor lids and caps for damages and cracks.

 Replace rotor lids or caps which show cracks or milky stains immediately.

WARNING! Damage to health due to rotor lids and caps which are not

aerosol-tight.

The aerosol tightness of rotor lids and caps is affected by autoclaving,

mechanical strain and contamination by chemicals.

 Check the integrity of the seals of the aerosol-tight caps before each use.

 Only work with undamaged and clean seals.

 Do not close aerosol-tight rotors and buckets for storing them.

Summary of content (5 pages)

PAGE 1
Instructions on see S-4-72/S-4-104 p. Tab. Fig. p. for use English Instructions (EN) for use Instructions for use S-4-72/S-4-104 English (EN) This document supplements the operation manual Centrifuge 5804/5804 R/5810/5810 R and does not replace it. Therefore, please also read the operating manual before starting up the rotors for the first time. You can find the current version on the Internet at www.eppendorf.com.
PAGE 2
Instructions for use 2 S-4-72/S-4-104 English (EN) 3 Centrifuging Follow the notes in the operating manual of the centrifuge on inserting the rotor, symmetric loading of buckets, max. load per bucket and the max. loading height/ tube length of the buckets. 3.1 Rotor S-4-104 only NOTICE! Buckets swinging out in the wrong direction. When working with the rotor S-4-104, using the wrong adapters with 500 mL Corning tubes may lead to the loss of samples or damage to the centrifuge.
PAGE 3
Instructions for use S-4-72/S-4-104 3 English (EN) 1. Fold the cap clamp to the open position (1). 2. Place the cap on the bucket and push the cap down so that the clamp is lifted slightly (2). 3. To transport the bucket, fold the clamp to the carrying position (3). 4. Fold the clamp beyond the latch into the close position. The clamp has only been folded correctly if there is an audible click (4).
PAGE 4
Instructions for use 4 S-4-72/S-4-104 English (EN) 3.2 Replacing the cap seal The seals of aerosol-tight caps can be replaced. Proceed as follows: Removing the seal 1. Lift the rubber seal from the groove using a blunt lever. You can, for instance, use the rounded side of a paper clip. Please take care not to damage the seal with the wire ends. 2. Carefully pull the seal out of the groove. 3.3 Inserting the seal NOTICE! Faulty sealing when the seal is handled incorrectly.  Uniformly insert the seal.
PAGE 5
Instructions for use S-4-72/S-4-104 English (EN) 4 Technical data Rotor S-4-104 Max. g-force (rcf)/speed (rpm): Round bucket 750 mL 3,214 x g/3,900 rpm Max. loading height / tube length: Round bucket 750 mL Please note the technical data in the operating manual of the centrifuge. Max. load per bucket: (Adapter, tube and contents) Round bucket 750 mL 1000 g Max. centrifugation radius: Round bucket 750 mL 18.9 cm Max. g-force (rcf)/speed (rpm): Round bucket 250 mL 3,234 x g/4,200 rpm Max.