OP rating Procedure for Pipettes EN) Operating Procedure for Pipettes Register your instrument! www.eppendorf.
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Table of contents Eppendorf SOP 3 English (EN) Table of contents 1 Test and pipette conditions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6 1.1 Balances . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6 1.1.1 Balance type . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6 1.1.2 Accuracy . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6 1.1.
Table of contents 4 Eppendorf SOP English (EN) 5 Leakage check . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15 5.1 Pipette leakage check . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15 6 Troubleshooting . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15 6.1 Potential error causes and corrections . . . . . . . . . . . . . . . . . . . . . . . 15 7 Adjustment . . . . . . . . . . . . . . .
Table of contents Eppendorf SOP 5 English (EN) 9 Technical specifications . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 37 9.1 Fixed-volume pipettes. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 37 9.1.1 Reference fixed . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 37 9.1.2 Reference 2 fixed . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 38 9.1.3 Research fixed. . . . . . . . . . . . . . . . . . . . . . . . . .
Test and pipette conditions 6 Eppendorf SOP English (EN) 1 Test and pipette conditions These test and pipetting conditions are valid for all Eppendorf pipettes and dispensers. Follow EN ISO 8655 "Volume-measuring equipment with pistons". For the calibration or adjustment of pipettes, the scales and measuring station should fulfill the following requirements: 1.1 Balances 1.1.1 Balance type Use semi-microbalances and microbalances to calibrate pipettes.
Test and pipette conditions Eppendorf SOP 7 English (EN) 1.2 Measuring station 1.2.1 Evaporation Take evaporation protection into account while carrying out the measurement. With volumes < 50 μl in particular, errors caused by the evaporation of the test liquid must be taken into consideration. This can be ensured by using a liquid trap or other equipment to prevent evaporation. 1.2.
Calibration 8 Eppendorf SOP English (EN) 2 2.1 Calibration Scope of testing Note concerning the nominal volume: The nominal volume of an adjustable-volume pipette is the largest volume to be set by the user and specified by the manufacturer. When using the Combitip with the Multipette, the nominal volume is the largest possible dispensing volume of the Combitip. This means that for the Multipette plus and Multipette M4, the nominal volume is 1/5 of the filling volume of the Combitip advanced.
Calibration Eppendorf SOP 9 English (EN) 2.1.4 Bottle-top dispenser and Top Buret For bottle-top dispensers and the Top Buret, check the nominal volume with 10 measured values. 2.2 Measurement 2.2.1 Work method 1. Place the selected pipette tip on the tip cone of the pipette or on the corresponding Combitip in the Multipette. 2.
Calibration 10 Eppendorf SOP English (EN) 2.2.3 Dispensing the test volume into the weighing vessel 1. Rest the filled tip up against the wall of the weighing vessel at an angle. 2. Dispense the test liquid slowly until the first stop (measuring stroke). 3. Press the control button to the second stop (blow-out) and dispense the remaining liquid in the tip (does not apply for dispensers and burets). 4. Hold down the control button and pull the tip up the vessel wall. 5.
Evaluation Eppendorf SOP 11 English (EN) 3 3.1 Evaluation Calculating the systematic error Mean value of the dispensed volume: n = Number of measured values To convert the measured values into volume values, use correction factor Z for the dependency of the test liquid on temperature and air pressure for each individual value (see Factor Z for distilled water on p. 36). Systematic error es in micro liter: Systematic error es in percent: 3.
Sterilization and cleaning 12 Eppendorf SOP English (EN) 4 Sterilization and cleaning Further notes on cleaning and sterilization can found in the relevant operating manual. 4.1 Sterilization Parameter for autoclaving • 121 °C • 20 minutes • 1 bar overpressure 4.1.1 Reference and Biomaster The Reference pipettes and the Biomaster are fully autoclavable. 1. Prior to autoclaving, unscrew the upper and lower part of the pipette apart by approx. one turn to enable the vapor to enter more easily. 2.
Sterilization and cleaning Eppendorf SOP 13 English (EN) 4.1.3 Research and Research pro With the Research and Research pro pipettes, the lower section is autoclavable. With single-channel models prior to autoclaving 1. Pull off the ejector sleeve with depressed ejector. 2. Unscrew the lower part of the pipette. With the Multi-channel versions, you can autoclave the entire lower section. After autoclaving, assemble the parts only after room temperature has been reached and the parts have been dried. 4.
Sterilization and cleaning 14 Eppendorf SOP English (EN) 4.1.7 Xplorer and Xplorer plus The lower part of theXplorer and Xplorer plus pipettes can be autoclaved. 1. Pull off the ejector sleeve with depressed ejector. 2. On the lower part of the pipette, push the ring labeled PUSH UP TO RELEASE upward until the lower part is released. After autoclaving, assemble the parts only after room temperature has been reached and the parts have been dried. 4.
Leakage check Eppendorf SOP 15 English (EN) 5 5.1 Leakage check Pipette leakage check In order to carry out a leakage check on a pipette, follow the procedure outlined below: 1. For adjustable-volume pipettes: set the nominal volume. 2. For volumes <20 μl pre-wet the tip several times. 3. Hold the pipette vertically with a full tip for approx. 30 sec. Do not touch the pipette tip. 4. Observe the meniscus of the liquid on the tip opening.
Troubleshooting 16 Eppendorf SOP English (EN) Error Cause Replace the Combitip advanced with a new Combitip advanced. Ensure that the Combitip advanced has temperature is uniform as heated up. the liquid expands when heated. Clean the piston and The piston is lubricate slightly. contaminated. The seal is contaminated. Disassemble the pipette, clean all seals, replace if necessary. Replace the piston and The piston is damaged. piston seal and lubricate slightly.
Adjustment Eppendorf SOP 17 English (EN) 7 7.1 Adjustment General information Prior to delivery, all pipettes are adjusted with distilled or de-ionized, degassed water under the conditions described in Chapters 1 to 3 and according to ISO 3696 (see Calibration on p. 8). If you have doubts regarding the accuracy of the volume entered, please check the following points first: • Is there a leak in the device (see Leakage check on p.
Adjustment 18 Eppendorf SOP English (EN) 7.2.1 Procedure Requirements Device, original Eppendorf tip, test liquid and ambient air must have the same temperature (15 – 30°C) at a constant temperature of ±0.5°C during the test (according to EN ISO 8655, Part 6). If your Research, Research plus or Reference has an adjustment, remove it before carrying out the adjustment. After adjusting, lock the adjustment opening with a new adjustment seal. Adjustment seals can be reordered (see operating manual). 1.
Adjustment Eppendorf SOP 19 English (EN) 7.2.2 Biomaster Auxiliary equipment • Supplied pipette wrench (order no. 4910 092.001) 1 0 2 0 7.2.3 1. Use the pipette wrench to adjust the volume display of the pipette, with the piston stroke unchanged, to the actual volume value of the measurement. 2. Pull off the pipette wrench. 3. Set the pipette to the target volume in the usual manner. 4. Mark the adjustment on the pipette. Reference variable Auxiliary equipment • Supplied pipette wrench (order no.
Adjustment 20 Eppendorf SOP English (EN) 7.2.4 Reference 2 adjustable-volume 7.2.4.1 User adjustment Changing the adjustment will change the volume by a specific value. Strictly speaking, the change only applies to the testing volume. Example: You readjust a 10 – 100 μL pipette, with a volume setting of 100 μL, by 1 μL (1 μL ≙ 1%). If the volume setting is 10 μL, the pipette is also adjusted by 1 μL (≙ 10%). 1. 2. 3. 4.
Adjustment Eppendorf SOP 21 English (EN) 7. Attach the red adjustment seal after the inspections. If the adjustment only applies to a specific liquid, mark the pipette accordingly. Use the labeling area on the pipette and mark it with the liquid and the volume. Carry out a gravimetric test for each adjustment change. Follow the EN ISO 8655-2 and EN ISO 8655-6 test procedures. 7.2.4.
Adjustment 22 Eppendorf SOP English (EN) 1000 µL ADJ 1 - 1 + 1. Check whether the adjustment display is set to "0". If the adjustment display is not set to “0”, it must be set to “0” first using the adjustment tool. In this case, instead of continuing with the factory adjustment changes, carry out a gravimetric test of the Reference2 with the adjustment display set to "0". 2. Use the plug to pierce the safety plug, and remove it. AD J Re 3. Insert the adjustment tool. AD J Re 4.
Adjustment Eppendorf SOP 23 English (EN) 5. Turn the adjustment tool to set the volume display. Set the volume display (from the volume setting used during the inspection to the calculated volume of the gravimetric test). The volume change applies to the entire volume range. For the volume change, you should initially set the value for 10% of the nominal volume of the value calculated during the gravimetric test.
Adjustment 24 Eppendorf SOP English (EN) 7.2.5 Research variable Auxiliary equipment • Supplied pipette wrench (order no. 3111 501.016) • Supplied red CAL seal 200 B D A Re se arc h C 20 0 1. Insert side D of the pipette wrench vertically into the lateral adjustment opening of the pipette grip. 2. Move the pipette wrench into the vertical position. 3. Turn the volume setting ring toward – or +. This adjusts the piston stoke of the pipette. The digital display does not change in the process. 4.
Adjustment Eppendorf SOP 25 English (EN) 7.2.6 Research plusadjustable volume - adjustment to environmental parameters Adjustment change for specific liquid densities, changed altitudes or pipette tips that are not used to calculate the random and systematic error. The Research plus was adjusted, tested and fitted with a gray adjustment seal with the abbreviation "ADJ" before delivery. The adjustment display on the side reads "0". Changing the adjustment will change the volume by a specific value.
Adjustment 26 Eppendorf SOP English (EN) 7.2.7 Research plus adjustable volume - change to the factory adjustment Auxiliary equipment • Supplied safety plug tool • Supplied pin to loosen the safety plug The factory adjustment can be changed with the corresponding accessories on a Research plus with variable volume setting. You can recognize a change to the factory adjustment that was made by the user of the Research plus from the red safety plug behind the ejector.
Adjustment Eppendorf SOP 27 English (EN) 2. Provide the volume setting ring and the ejector with a common mark with a pen. This mark is used for orientation purposes when changing the factory adjustment. When changing the factory adjustment, you can turn the volume setting ring without changing the volume display. The mark on the volume setting ring and the ejector informs you how far you have moved from the factory setting. 3. Keep the ejector pressed and remove the safety plug with the pin.
Adjustment 28 Eppendorf SOP English (EN) 4. Continue to keep the ejector pressed. Insert the safety plug tool in such a way that the counter locking mechanism is pushed down. 5. Turn the volume setting ring slightly to change the volume. Proceed as shown in the illustration.
Adjustment Eppendorf SOP 29 English (EN) 6. This results in the following approximate volume changes: single-channel Nominal volume Color code 2.5 μL Dark gray 10 μL Medium gray 20 μL light gray 20 μL Yellow 100 μL Yellow 200 μL Yellow 300 μL Orange 1,000 μL Blue 5 mL Purple 10 mL Turquoise multi-channel Nominal volume Color code 10 μL Medium gray 100 μL Yellow 300 μL Orange +½ revolution 0.106 μL +¼ revolution 0.053 μL –¼ revolution -0.053 μL –½ revolution -0.106 μL 0.53 μL 0.27 μL -0.
Adjustment 30 Eppendorf SOP English (EN) 7. Push the locking mechanism upwards and carry out a gravimetric test of the changes made. 8. If the values measured during the gravimetric test meet your requirements: insert the red safety plug in the tool in the opening of the Research plus and snap it off the tool. The red safety plug on the pipette indicates that the Research plus has been adjusted and calibrated by the user.
Adjustment Eppendorf SOP 31 English (EN) 7.2.8 Research Multi-channel Auxiliary equipment • Supplied pipette wrench (order no. 3111 501.016) • Supplied red ADJ seal B D A C 1. Insert the D side of the pipette wrench horizontally into the adjustment opening on the side of the pipette grip. 2. Move the pipette wrench into the vertical position. 3. Turn the volume setting ring toward – or +. This adjusts the piston stoke of the pipette. The digital display does not change in the process. 4.
Adjustment 32 Eppendorf SOP English (EN) 7.2.10 Varispenser plus Turn the fine adjustment toward + or –. A revolution corresponds to the smallest dispensing step: Reduce volume: Turn toward -. Increase volume: Turn toward +. The factory adjustment was carried out at 20 °C, using double-distilled, degassed water. 7.2.11 Xplorer and Xplorer plus The re-adjustment of the Xplorer pipette is described on the CD which is enclosed with the pipette.
Adjustment Eppendorf SOP 33 English (EN) 7.3 Adjusting fixed-volume pipettes The adjustment process for fixed-volume pipettes is identical to the adjustment process for variable pipettes (see Adjusting adjustable-volume pipettes on p. 17). For fixed-volume pipettes, 10 measured values of the nominal volume are checked. 7.3.1 Reference fixed Auxiliary equipment • Adhesive label as an adjusting aid for the basic setting • Supplied pipette wrench (order no. 4910 092.001) 1.
Adjustment 34 Eppendorf SOP English (EN) 7.3.2 Reference2 Adjustment is carried out as described for the Reference 2 adjustable-volume (see p. 20). 7.3.3 Research fixed Auxiliary equipment • Supplied pipette wrench (order no. 3111 501.016) B D A 1. Insert side D of the pipette wrench vertically into the lateral adjustment opening of the pipette grip. 2. Move the tool into the vertical position. 3. Turn the volume setting ring toward – or +. This adjusts the piston stoke of the pipette.
Adjustment Eppendorf SOP 35 English (EN) 7.3.4 Research plus fixed Adjustment is carried out as described for the Research plus adjustable-volume(see p. 25). 7.4 Physical influences of liquids It is possible to adjust the previously described devices for a volume of a liquid with a different density than water so that the displayed volume value corresponds to the pipetted volume.
Factor Z for distilled water 36 Eppendorf SOP English (EN) 8 8.1 Factor Z for distilled water Factor Z overview table Factor Z (μl/mg) in accordance with EN ISO 8655 for distilled water depending on test temperature and air pressure: Temperature (°C) 15 15.5 16 16.5 17 17.5 18 18.5 19 19.5 20 20.5 21 21.5 22 22.5 23 23.5 24 24.5 25 25.5 26 26.5 27 27.5 28 28.5 29 29.5 30 80 1.0017 1.0018 1.0019 1.0020 1.0021 1.0022 1.0022 1.0023 1.0024 1.0025 1.0026 1.0027 1.0028 1.0030 1.0031 1.0032 1.0033 1.0034 1.
Technical specifications Eppendorf SOP 37 English (EN) 9 Technical specifications The following specifications are valid for the following conditions: Liquid: Distilled or deionized water Reference temperature: 20 °C to 25 °C ±0.5 °C Number of determinations: 10, in accordance with EN ISO 8655 with original Eppendorf pipette tips Technical specifications subject to change! 9.1 Fixed-volume pipettes 9.1.1 Reference fixed Model 1 μL 2 μL 5 μL Test tip epT.I.P.S.
Technical specifications 38 Eppendorf SOP English (EN) 9.1.2 Reference 2 fixed Fixed-volume Reference2 Model Test tip Eppendorf AG error limits epT.I.P.S. Color code Error Volume range Systematic error Random error Length ±% ± µL ±% ± µL 1 μL Dark gray ±2.5 ±0.025 ±1.8 ±0.018 0.1 μL – 10 μL 2 μL ±2.0 ±0.04 ±1.2 ±0.024 34 mm 5 μL Medium gray ±1.2 ±0.06 ±0.6 ±0.03 0.1 μL – 20 μL 10 μL ±1.0 ±0.1 ±0.5 ±0.05 40 mm 20 μL light gray ±0.8 ±0.16 ±0.3 ±0.06 0.5 μL – 20 μL L 46 mm 10 μL Yellow ±1.2 ±0.12 ±0.6 ±0.
Technical specifications Eppendorf SOP 39 English (EN) 9.1.3 Model 10 μL 20 μL 25 μL Research fixed Test tip epT.I.P.S. Color code Volume range Length Yellow 2 - 200 μL 53 mm Error limits Error Systematic error Random error ±% ± µL ±% ± µL ±1.2 ±0.12 ±0.6 ±0.06 ±1.0 ±0.2 ±0.3 ±0.06 ±1.0 ±0.25 ±0.3 ±0.075 50 μL ±0.7 ±0.35 ±0.3 ±0.15 100 μL ±0.6 ±0.6 ±0.2 ±0.2 ±0.6 ±1.2 ±0.2 ±0.4 ±0.6 ±1.5 ±0.2 ±0.5 ±0.6 ±3.0 ±0.2 ±1.0 ±0.6 ±6.0 ±0.2 ±2.
Technical specifications 40 Eppendorf SOP English (EN) 9.1.4 Research plus fixed Research plus fixed volume Model Test tip epT.I.P.S. Color code Volume range Length Eppendorf AG error limits Error Systematic error Random error ±% ± µL ±% ± µL 10 μL Medium gray 0.1 μL – 20 μL 40 mm ±1.2 ±0.12 ±0.6 ±0.06 20 μL light gray 0.5 μL – 20 μL L 46 mm ±0.8 ±0.16 ±0.3 ±0.06 10 μL Yellow 2 μL - 200 μL 53 mm ±1.2 ±0.12 ±0.6 ±0.06 ±1.0 ±0.2 ±0.3 ±0.06 20 μL 25 μL ±1.0 ±0.25 ±0.3 ±0.
Technical specifications Eppendorf SOP 41 English (EN) 9.2 Adjusting adjustable volume pipettes 9.2.1 Reference variable Model Test tip Testing Error limits epT.I.P.S. Color code volume Error Volume range Systematic error Random error Length ±% ± µL ± % ± µL 0.1 - 2.5 μL Dark gray 0.25 μL ±12.0 ±0.03 ±6.0 ±0.015 0.1 - 10 μL 1.25 μL ±2.5 ±0.031 ±1.5 ±0.019 34 mm 2.5 μL ±1.4 ±0.035 ±0.7 ±0.018 0.5 - 10 μL light gray 1 μL ±2.5 ±0.025 ±1.8 ±0.018 0.5 – 20 μL 5 μL ±1.5 ±0.075 ±0.8 ±0.04 46 mm 10 μL ±1.
Technical specifications 42 Eppendorf SOP English (EN) 9.2.2 Reference 2 adjustable-volume Reference2 single-channel variable Model Test tip Testing epT.I.P.S. Color code volume Volume range Length 0.1 μL – 2.5 μL Increment: 0.002 μL Dark gray 0.1 μL – 10 μL 34 mm 0.5 μL – 10 μL Increment: 0.01 μL Medium gray 0.1 μL – 20 μL 40 mm 2 μL – 20 μL Increment: 0.02 μL light gray 0.5 μL – 20 μL L 46 mm 2 μL – 20 μL Increment: 0.02 μL Yellow 2 μL - 200 μL 53 mm 10 μL – 100 μL Increment: 0.
Technical specifications Eppendorf SOP 43 English (EN) Reference2 single-channel variable Model Test tip Testing epT.I.P.S. Color code volume Volume range Length 0.25 mL – 2.5 mL Red Increment: 0.25 mL – 2.5 mL 0.002 mL 115 mm 0.5 mL – 5 mL Increment: 0.005 mL Purple 0.1 mL – 5 mL 120 mm 1 mL – 10 mL Increment: 0.01 mL Turquoise 1 mL – 10 mL 165 mm Eppendorf AG error limits Error Systematic Random error error ± % ± mL ± % ± mL 0.25 mL ±4.8 ±0.012 ±1.2 ±0,003 1.25 mL ±0.8 ±0,010 ±0.2 ±0.0025 2.5 mL ±0.
Technical specifications 44 Eppendorf SOP English (EN) 9.2.3 Research adjustable-volume Model Test tip Testing epT.I.P.S. Color code volume Volume range Length 0.1 - 2.5 μL Dark gray 0.1 - 10 μL 34 mm 0.5 - 10 μL light gray 0.5 – 20 μL L 46 mm 2 - 20 μL Yellow 2 - 200 μL 53 mm 10 - 100 μL Yellow 2 – 200 μL 53 mm 20 - 200 μL Yellow 2 - 200 μL 53 mm 100 – 1 000 μL Blue 0.05 – 1 mL 71 mm 0.5 – 5 mL Purple 0.1 – 5 mL 120 mm 1 – 10 mL Turquoise 1 – 10 mL 165 mm 0.25 μL 1.25 μL 2.
Technical specifications Eppendorf SOP 45 English (EN) 9.2.4 Research pro Model Test tip Testing epT.I.P.S. Color code volume Volume range Length 0.5 - 10 μL light gray 0.5 – 20 μL L 46 mm 5 - 100 μL Yellow 2 - 200 μL 53 mm 20 – 300 μL Orange 20 – 300 μL 55 mm 50 - 1 000 μL Blue 50 - 1 000 μL 71 mm 500 – 5 000 μL Purple 0.1 – 5 mL 120 mm Error limits Error Systematic Random error error ±% ± µL ±% ± µL 1 μL ±2.5 ±0.025 ±1.8 ±0.018 5 μL ±1.5 ±0.075 ±0.8 ±0.04 10 μL ±1.0 ±0.1 ±0.4 ±0.
Technical specifications 46 Eppendorf SOP English (EN) 9.2.5 Research plus adjustable-volume Research plus adjustable single-channel Model Test tip Testing epT.I.P.S. Color code volume Volume range Length 0.1 μL – 2.5 μL Increment: 0.002 μL Dark gray 0.1 μL – 10 μL 34 mm 0.5 μL – 10 μL Increment: 0.01 μL Medium gray 0.1 μL – 20 μL 40 mm 2 μL – 20 μL Increment: 0.02 μL light gray 0.5 μL – 20 μL L 46 mm 2 μL – 20 μL Increment: 0.
Technical specifications Eppendorf SOP 47 English (EN) Research plus adjustable single-channel Testing Model Test tip epT.I.P.S. Color code volume Volume range Length 0.5 μL – 5 mL Increment: 0.005 mL Purple 0.1 μL – 5 mL 120 mm 1 μL – 10 mL Increment: 0.01 mL Turquoise 1 μL – 10 mL 165 mm 9.2.6 Eppendorf AG error limits Error Systematic Random error error ± % ± µL ±% ± µL 0.5 mL ±2.4 ±12 ±0.6 ±3.0 2.5 mL ±1.2 ±30 ±0.25 ±6.0 5.0 mL ±0.6 ±30 ±0.15 ±8.0 1.0 mL ±3.0 ±30 ±0.6 ±6.0 5.0 mL ±0.8 ±40 ±0.
Technical specifications 48 Eppendorf SOP English (EN) 9.2.8 Xplorer and Xplorer plus pipette Increment Pipette tip Color code Volume range Length µL 0.5 μL – 10 μL Increment: 0.01 μL Medium gray 0.1 μL – 20 μL 40 mm 5 μL– 100 μL Increment: 0.1 μL Yellow 2 μL - 200 μL 53 mm 15 μL – 300 μL Increment: 0.2 μL Orange 15 μL – 300 μL 55 mm 50 μL – 1 000 μL Increment: 1 μL Blue 50 μL – 1000 μL 71 mm mL 0.25 mL – 5 mL Increment: 0.005 mL mL Purple 0.1 mL – 5 mL 120 mm 0.5 mL – 10 mL Increment: 0.
Technical specifications Eppendorf SOP 49 English (EN) 9.3 Multi-channel pipettes 9.3.1 Research Model Research8-channel 0.5 - 10 μL Research12-channel 0.5 - 10 μL Research8-channel 10 - 100 μL Research12-channel 10 - 100 μL Research8-channel 30 - 300 μL Research12-channel 30 - 300 μL Volume Error limits Test tip epT.I.P.S. Color in µL Error code Systematic Random error Volume range error Length ±% ± µL ±% ± µL light gray 1 ±8.0 ±0.08 ±5.0 ±0.05 0.5 – 20 μL 5 ±4.0 ±0.2 ±2.0 ±0.1 46 mm 10 ±2.0 ±0.
Technical specifications 50 Eppendorf SOP English (EN) 9.3.2 Model Research pro Test tip Testing epT.I.P.S. Color code volume Volume range Length Research pro light gray 8-channel /12-channel 0.5 – 20 μL L 0.
Technical specifications Eppendorf SOP 51 English (EN) 9.3.3 Research plus Research plus adjustable multi-channel Model Test tip Testing Eppendorf AG error limits epT.I.P.S. Color code volume Error Volume range Systematic Random Length error error ± % ± µL ± % ± µL 0.5 μL – 10 μL Medium gray 0.5 μL ±12 ±0.06 ±8.0 ±0.04 Increment: 0.1 μL – 20 μL 1 μL ±8.0 ±0.08 ±5.0 ±0.05 0.01 μL 40 mm 5 μL ±4.0 ±0.2 ±2.0 ±0.1 10 μL ±2.0 ±0.2 ±1.0 ±0.1 10 μL – 100 μL Yellow 10 μL ±3.0 ±0.3 ±2.0 ±0.
Technical specifications 52 Eppendorf SOP English (EN) 9.3.4 Reference2 Reference2 Variable multi-channel (8-channel/12-channel) Model Test tip Testing Eppendorf AG error limits epT.I.P.S. Color code volume Error Volume range Systematic Random Length error error ± % ± µL ± % ± µL 0.5 μL – 10 μL Medium gray 0.5 μL ±12.0 ±0.06 ±8.0 ±0.04 0.1 μL – 20 μL 1 μL ±8.0 ±0.08 ±5.0 ±0.05 40 mm 5 μL ±4.0 ±0.2 ±2.0 ±0.1 10 μL ±2.0 ±0.2 ±1.0 ±0.1 10 μL – 100 μL Yellow 10 μL ±3.0 ±0.3 ±2.0 ±0.2 2 μL - 200 μL 50 μL ±1.
Technical specifications Eppendorf SOP 53 English (EN) 9.3.5 Xplorer and Xplorer plus pipette Increment Pipette tip Color code Volume range Length µL 0.5 μL – 10 μL Increment: 0.01 μL Medium gray 0.1 μL – 20 μL 40 mm 5 μL – 100 μL Increment: 0.1 μL Yellow 2 μL –200 μL 53 mm 15 μL – 300 μL Increment: 0.2 μL Orange 15 μL –300 μL 55 mm 50 μL – 1200 μL Increment: 1 μL Green 50 μL–1250 μL 76 mm Testing volume Error limits Error Systematic Random error error µL % µL % µL 1 μL ±5.0 ±0.05 ±3.0 ±0.
Technical specifications 54 Eppendorf SOP English (EN) 9.4 Multipette The following specifications for the Multipette plus, Multipette stream / Multipette Xstream, Multipette M4 apply to the following conditions: • Using the Combitips advanced • Liquid: Distilled or deionized water • Reference temperature: 20 to 25 °C, ±0.
Technical specifications Eppendorf SOP 55 English (EN) 9.4.2 MultipetteM4 Combitip advanced Testing volume 0.1 mL white Increment: 1 μL 0.2 mL light blue Increment: 2 μL 0.5 mL purple Increment: 5 μL 1 mL yellow Increment: 10 μL 2.5 mL green Increment: 25 μL 5 mL blue Increment: 50 μL 10 mL orange Increment: 0.1 mL 2 μL 20 μL Error limits Error Systematic error Random error ±% ± µL ±% ± µL ±1.6 ±0.032 ±3.0 ±0.06 ±1.0 ±0.2 ±2.0 ±0.4 4 μL 40 μL ±1.3 ±0.8 ±0.052 ±0.32 ±2.0 ±1.5 ±0.08 ±0.
Technical specifications 56 Eppendorf SOP English (EN) 9.4.3 Multipette stream / Multipette Xstream Combitip advanced Volume range 0.1 mL 1 μL - 100 μL (white piston) Increment: 0.1 μL Testing volume 10 μL 50 μL 100 μL 0.2 mL 2 μL - 200 μL 20 μL (blue piston) 100 μL Increment: 0.2 μL 200 μL 0.5 mL 5 μL - 500 μL 50 μL Increment: 0.5 μL 250 μL 500 μL 1 mL 10 μL – 1 000 μL 100 μL Increment: 1 μL 500 μL 1,000 μL 2.5 mL 25 μL – 2 500 μL 250 μL Increment: 2.
Technical specifications Eppendorf SOP 57 English (EN) 9.5 Varispenser / Top Buret The following specifications for Varispenser and Top Buret are valid for the following conditions: Liquid: Distilled or deionized water Reference temperature: 20 to 25 °C, ±0.5 °C unchanging Number of determinations: 10, in accordance with EN ISO 8655 9.5.1 Varispenser and Varispenser plus Setting range Tested volume Systematic error (inaccuracy) Random error (imprecision) 0.5 – 2.50 mL 2.5 mL ± 0.6 % ≤ 0.
Technical specifications 58 Eppendorf SOP English (EN) 9.6.1 Air-cushion pipettes, fixed and adjustable volume Nominal volume 1 μl 2 μl 5 μl 10 μl 20 μl 50 μl 100 μl 200 μL 500 μl 1 000 μL 2 000 μL 5 000 μL 10 000 μL Error limits Measurement errors Systematic Random ±% ± µL ±% ± µL ±5.0 % ±0.05 μl ±5.0 % ±0.05 μl ±4.0 % ±0.08 μl ±2.0 % ±0.04 μl ±2.5 % ±0,125 μL ±1.5 % ±0.075 μl ±1.2 % ±0,12 μL ± 0.8 % ±0.08 μl ±1.0 % ±0.2 μl ±0.5 % ±0.1 μl ±1.0 % ±0.5 μl ±0.4 % ±0.2 μl ± 0.8 % ±0.8 μl ±0.3 % ±0.
Technical specifications Eppendorf SOP 59 English (EN) 9.6.3 Dispenser (Multipette) Nominal volume 0.001 mL 0,002 ml 0,003 ml 0,01 ml 0,02 ml 0,05 ml 0,1 ml 0.2 ml 0.5 ml 1 ml 2 ml 5 mL 10 mL 25 ml 50 mL 100 ml 200 ml Error limits Measurement errors Systematic Random ±% ± µL ±% ± µL ±5.0 % ±0.05 μl ±5.0 % ±0.05 μl ±5.0 % ±0.1 μl ±5.0 % ±0.1 μl ±2.5 % ±0.075 μl ±3.5 % ±0,11 μL ±2.0 % ±0.2 μl ±2.5 % ±0.25 μl ±1.5 % ±0.3 μl ±2.0 % ±0.4 μl ±1.0 % ±0.5 μl ±1.5 % ±0.75 μl ±1.0 % ±1,0 μL ±1.0 % ±1,0 μL ±1.
Technical specifications 60 Eppendorf SOP English (EN) 9.6.4 Single-stroke dispenser (Varispenser) Nominal volume 0.01 ml 0.02 ml 0.05 ml 0.1 ml 0.2 ml 0.5 ml 1 ml 2 ml 5 mL 10 mL 25 ml 50 mL 100 ml 200 ml 9.6.5 Piston stroke burets Nominal volume <1 mL 2 ml 5 mL 10 mL 20 ml 25 ml 50 mL 100 ml Error limits Measurement errors Systematic Random ±% ± µL ±% ± µL ±2.0 % ±0.2 μl ±1.0 % ±0.1 μl ±2.0 % ±0.4 μl ±0.5 % ±0.1 μl ±1.5 % ±0.75 μl ±0.4 % ±0.2 μl ±1.5 % ±1.5 μl ±0.3 % ±0.3 μl ±1.0 % ±2.0 μL ±0.
Index Eppendorf SOP 61 English (EN) Index Error limits....................................... 57 Evaporation........................................ 7 A Accuracy............................................ 6 F Adjustable-volume pipettes Adjust......................................... 18 Factor Z Table........................................... 36 Adjusting adjustable volume pipettes Technical specifications............. 41 Fixed-volume pipettes Adjust .........................................
Index 62 Eppendorf SOP English (EN) R Technical specifications ............. 57 Random error .................................. 11 Volume errors .................................. 17 S W Scope of testing Adjusting adjustable volume pipettes ........................................ 8 Bottle-top dispenser, Top Buret... 9 Multi-channel pipettes ................. 8 Multipette .................................... 8 Work method ..................................... 9 Sterilization ......................
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