Leica EG1150 H Paraffin Embedding Station Instruction Manual Leica EG1150 H V2.0 English - 10/2002 Always keep this manual near the instrument. Read carefully prior to operating the instrument.
IMPORTANT NOTE The information, numerical data, notes and value judgments contained in this manual represent the current state of scientific knowledge and state-ofthe-art technology as we understand it following thorough investigation in this field. We are under no obligation to update the present manual periodically and on an ongoing basis according to the latest technical developments, nor to provide our customers with additional copies, updates etc. of this manual.
Table of contents 1. 1.1 1.2 1.3 1.4 Important notes .......................................................................................................................................................... 5 Symbols used in the text and their meanings ..................................................................................................................................................................... 5 Specified use and application ...........................................................
1. 1.1 Symbols used in the text and their meanings Warnings and cautions appear in a gray box and are marked by a warning triangle . Notes, i.e. important information for the user, appear in a gray box and are marked with the symbol . Solvents and reagents that are inflammable are marked with this symbol. Important notes 1.2 Specified use and application The Leica EG1150H is a modern paraffin embedding station with microprocessor control.
2. Safety Please pay particular attention to the safety regulations and safety instructions in this section. Please also read this information, even if you are familiar with handling and using a Leica instrument. 2.1 Safety regulations This instruction manual contains important instructions and information regarding operational safety and maintenance of the instrument. The instruction manual is an important part of the product.
2. Safety Safety instructions safety regulations on the instrument itself Safety regulations marked with a warning triangle on the instrument itself mean that when operating or exchanging respective parts of the instrument, the correct operating steps as described in the instruction manual supplied, must be adhered to. Non-observance can cause accidents, injuries and/or damage to the instrument/accessories. Certain surfaces of the instrument are hot during operation under normal conditions.
2. Safety Hazards servicing and cleaning Switch off the instrument each time before servicing and pull out the mains plug. When using cleaners, please comply with the safety instructions of the manufacturer and the laboratory safety regulations. Before changing defective fuses, the instrument has to be disconnected from the mains. Only fuses that are easily accessible may be replaced by the user.
3. Instrument components and specifications 3.1 Specifications General data Approvals: Power voltages: Input: Class of protection1: Degree of pollution1: Overvoltage category: Operating temperature range: Working temperatures: Relative air humidity: 1) Fuses The approval symbols relating to this instrument are located on the rear of the instrument next to the nameplate. 100-120 V, 50/ 60 Hz 230-240 V, 50/ 60 Hz 1100 VA I 2 II +18 °C to + 40°C 55 °C to 70 °C, adjustable in 5 degree (K) increments.
3. Instrument components and specifications 3.2 Overview instrument parts Standby switch Paraffin reservoir with lid Control panel Work area illumination Dispenser Cassette warmer lid Cassette warmer Forceps holder Work area Mold warmer lid Mold warmer Paraffin collection tray left Paraffin collection tray right Refrigeration spot Fig. 1 10 Instruction Manual V 2.
3. Instrument components and specifications Instrument rear view Secondary fuses F1-F3 Secondary fuses F4-F5 Heat sink Port for power cord Port for foot switch Sliding elements 3.3 Instrument specifications Fig. 2 Paraffin reservoir with a capacity of 3 liters. The paraffin flow is activated by means of a height-adjustable, pivotable clip activated either manually by the mold or a foot switch (optional). Controllable flow rate.
4. Commissioning 4.1 Unpacking and installation The unpacking instructions are attached to the outside of the transport crate. Fig. 3 shows the design of the original packaging. The numbers indicate the sequence of disassembling and reassembling. To lift up the instrument (6), hold it at the front and rear of the base plate (Fig. 4) and lift it from the foam padding (8). Important! Lifting the instrument by the dispenser (10) or paraffin reservoir (11) can cause serious damage.
4. Commissioning 4.2 Location conditions Stable, vibration-free laboratory table with horizontal, flat table top, as far as possible vibration-free ground. The instrument must not be placed in the vicinity of the air outlet of an air conditioner and must be shielded from strong sunlight (window). To ensure a fully functional heat sink, there must be gap of at least 15 cm behind the instrument. The vicinity of the work area must be free of oil and chemical vapors.
4. Commissioning 4.4 Necessary assembly work Install the following accessories and make the appropriate adjustments to make the instrument ready for use: Install accessories. Adjust instrument feet if necessary. Install magnifier (optional). Connect foot switch (optional). Establish the electrical connections. Install accessories Install mold warmer (2) and cassette warmer (3).
4. Commissioning Installing the accessories 9 8 7 4 5 2 11 11 10 1 3 6 6 Fig.
4. Commissioning 4.5 Optional accessories Magnifying glass The magnifying glass provides a magnified view of the work area. When properly adjusted, an enlarged view of the dispenser and refrigeration spot is available. 5 1 1 2 3 4 5 4 3 Fig. 6 Installing the magnifying glass On the dispenser (5) a tap hole (1) is provided, which is closed with a nylon screw (2). Remove the screw (2) with a screwdriver and store it in a safe place.
4. Commissioning 4.6 Electrical connection The instrument MUST be connected to a grounded mains socket. The instrument is supplied with a set of different power cords. Only the power cord intended for the local power supply (socket) may be used. Do not use an extension cord. 1 4 6 2 3 5 Fig. 8 Before connecting the power cord to the foot switch, ensure that the standby switch (1) (control panel, front) is set to O (O = OFF).
5. Operation 5.1 Instrument parts/functions 1 2 11 Recycled paraffin may not be used in the Leica EG1150H due to the danger of contamination. 1 14 13 13 2 12 Fig. 9 The flow cannot be stopped completely with the metering screw (11). It must not be turned whilst cold! 18 Paraffin reservoir (1) The paraffin reservoir has a capacity of 3 liters. The paraffin temperature is adjustable between 55 °C and 70 °C in 5 degree (K) increments.
5. Operation Work area (3) The temperature of the work area can be adjusted between 55 °C and 70 °C in 5 degree (K) increments. This includes the embedding area, forceps holder (5) and the refrigeration spot (4). A groove around the work area and several drain holes (15) are provided to allow liquid paraffin to drain rapidly. 5 3 4 3 Refrigeration spot (4) The refrigeration spot is an integral part of the work area.
5. Operation Paraffin collection tray (8) Two indirectly-heated paraffin collection trays for excess paraffin are located under the work surface. The paraffin collection tray must be emptied daily. To prevent contamination, do not reuse paraffin collected in this tray. If the instrument is operated without the paraffin collection trays there is risk of burning.
5. Operation Standby switch (8) After the unit has been commissioned, the standby switch should only be used if the unit is to be switched off for a longer period of time. 21 8 9 20 In daily routine operation, the ON/OFF button on the control panel should be used. Pressing the ON/OFF button (20) switches the instrument into active standby mode. All displays are switched off, only the LED (21) of the ON/OFF button remains lit.
5. Operation Foot switch (22) optional The foot switch (22) actuates the magnet valve of the dispenser. 22 11 It must be connected as described in Chapter 4.6. The plug of the foot switch MUST be screwed onto the socket. Otherwise hot paraffin may escape even when the foot switched is not being pressed. 14 12 Pressing the foot switch opens the valve, releasing it closes it. This keeps the operators hands free to work with the instrument. The flow volume can be adjusted with the metering screw (11).
5. Operation 5.2 Switching the instrument on Fig. 14 Activate the standby switch (left-hand side, next to control panel) (see Figs. 14, 16). All LEDs of the operating panel light up briefly and the installed software version is shown on the display for around 2 seconds. All displays go out, the unit changes to standby mode. The green LED in the ON/OFF button indicates that the instrument is ready to operate. Press the ON/OFF button down for approx. 2 seconds to switch over to the operating mode.
5. Operation 5.3 Control panel functions The control panel beside the standby switch (8) consists of a foil keyboard with push buttons, LEDs and a single-line display. It is divided into two control areas: temperature setting and display and instrument control and programming. 8 Temperature control and display Set time LED display for the individual heated areas 6 5 1 2 3 24 Display: standard indication is real time Fig.
5. Operation 5.4 Operating modes 21 Standby mode The display and all LEDs are switched off in standby mode. The unit switches on or off automatically at the programmed start and ending times. Only the green LED (21) in the ON/OFF button indicates that the instrument is ready to operate. Enabling operating mode Hold down the ON/OFF button for approx. 2 seconds. The instrument switches from standby mode to operating mode.
5. Operation 5.5 Time-program control The value shown in the display must be set to the current local time to ensure the correct operation of the time-program control. Setting the time Press the CLOCK button as many times as required until the green TIME LED lights up. Set the current time with the / buttons. The required value is reached faster when holding down the button in question.
5. Operation Weekdays/working days The automatic switch-on feature is linked with the individual weekdays. It is therefore necessary to define the days for which the automatic switchon feature shall operate. Only on those weekdays that have been defined as working days, the instrument will be at the required temperature and ready to operate. Setting the current weekday: Press the DAY button as many times as required until the green TODAY LED lights up.
5. Operation 5.6 Instrument heater Setting temperature values The temperatures for the four heated areas can be adjusted separately in a range from 55 °C to 70 °C in 5 degree (K) increments. 1 2 3 When setting the temperature, please observe the paraffin manufacturers specifications for the maximum permissible temperature. 4 Fig.
5. Operation Advance times Display To ensure that the instrument is ready (all working temperatures reached) at the programmed start time, the various sections are activated in advance as follows: Paraffin reservoir: heating starts 4 hours before work starts. Work area: heating starts 4 hours before work starts. Dispenser tube: heating starts 1 hour before work starts. Refrigeration spot: refrigeration begins 1 hour before work starts.
6. Cleaning and maintenance 6.1 Cleaning the instrument Do not use xylene for cleaning. Xylene vapors are heavier than air and can ignite at a considerable distance from the source of heat. Fire hazard! To avoid scratching the surface of the instrument only the plastic spatula that is supplied with it should be used for cleaning on no account metal tools! Work area All common laboratory cleaning products suitable for the removal of paraffin (e.g.
6. Cleaning and maintenance Paraffin collection tray Before the paraffin collection tray drawers can be emptied, any excess paraffin on the work area must be removed with cellulose wadding in order to prevent any paraffin from penetrating into the instrument. Use care with paraffins with a low melting point - risk of burning when removing the paraffin collection trays due to liquid paraffin. Only remove and empty the paraffin collection trays while they are warm.
7. Troubleshooting 7.1 Possible faults This chapter will help to correct problems that may occur when working with the Leica EG1150H. If a problem cannot be remedied by following the instructions in this chapter, please contact your Leica technical service center. For further instructions, please refer to Chapter 8. The table below includes the most common problems that may occur when working with the instrument, including possible causes and corrective action.
7. Troubleshooting Problem Possible cause Corrective action 3. Paraffin reservoir Paraffin does not melt or melts only very slowly. - Selected temperature too low. - Increase temperature for paraffin reservoir. - No enhanced mode set. - Activate enhanced mode. (see Chapter 5.6) - Temperature indication incorrect, or no heating of paraffin reservoir. - Check temperature settings and repeat if necessary. - Start-of-work time selection incorrect. - Check start-of-work time.
7. Troubleshooting Problem 5. Illumination does not work. 6. Individual heated areas (despite correct temperature indication) heating does not work. 7. Error message in display On the display the indication is shown. Program and time are lost. Possible cause Corrective action - Cap of the work area halogen lamp incorrectly positioned. - Check adjustment of lamp cap. (see Figures 12 / 23) - Bulb defective. - Replace bulb (see Chapter 7.2) - Fuse defective. - Replace fuse (see Chapter 7.
7. Troubleshooting 7.2 Replacing the halogen lamp Only use the original replacement lamp delivered with the maintenance kit. Before you replace the lamp, switch off the unit at the standby switch and disconnect the mains plug. To replace the halogen lamp, please proceed as shown in Fig. 23: 1 2 5 1 2 3 4 1. Turn the upper, moveable cap on the lamp (1) until the markings are in alignment as shown in the figure. 2. Remove cap this can be done only in the position shown in . 3.
7. Troubleshooting 7.3 Changing a fuse Before changing a fuse switch the instrument off and pull out the mains plug. Use ONLY the provided replacement fuses. Important! The instrument has a number of circuits that are protected by fuses with different values. Observe the following instructions exactly to ensure that the correct spare fuse is used for the matching fuse holder. The instrument has five electrical areas with separate secondary fuse protection.
7. Troubleshooting Select the correct spare fuse This marking (2A) on the fuse and the labeling next to the fuse holder on the rear of the instrument MUST agree! Fig. 25 The metal caps on the spare fuses are labeled the labeling looks similar to that shown in the figure above, but can vary depending on the type of fuse. The label information which is important for the correct use of the fuse is its maximum current (in the marked example: T2A).
8. Warranty and service Warranty Leica Microsystems Nussloch GmbH guarantees that the contractual product delivered has been subjected to a comprehensive quality control procedure based on the Leica in-house testing standards, and that the product is faultless and complies with all technical specifications and/or agreed characteristics warranted. The scope of the warranty is based on the content of the concluded agreement.
