Specifications
Instruction Manual    Alpha DO 500 
14 
4.2  Calibration in mg/l or ppm of Oxygen 
The DO Transmitter is capable of calibration of up to 2 points for dissolved 
oxygen. All new calibration values will automatically override existing 
calibration data. Make sure the unit of measurement is set to mg/l or ppm. 
(Refer Section 5.2 for switching unit of measurements). 
If you wish to abort the DO calibration, press  and  keys simultaneously 
and the DO Transmitter reverts to measurement mode. 
4.2.1 1-Point Calibration 
The 1-point calibration is done at high-point  using  8.24 mg/l of dissolved 
oxygen solution. 
Measured value should be
within +/- 50 % of high-point 
Calibration error occurs if measured value
is not within +/- 50% of high-point 
during 1-point calibration 
2
CAL
HOLD
1
ENTENTENT
CAL
HOLD
2
CAL
HOLD
2
CAL
HOLD
3
mg/l
MEAS
mg/l
°C
ATC
READY
4
CAL
HOLD
mg/l
READY
ERR
+
11
From measurement mode press CAL key to enter calibration mode as 
described in section 4.1. The LCD shows ‘CAL Do’. Press ENT key to 
begin calibration. 
2
Select 1-point calibration: The display shows ‘1Pt CAL’. If ‘2Pt CAL’ is 
shown, press  or  key to select ‘1Pt CAL’. 
Press ENT key to confirm the selection. 
3
The buffer annunciator   appears in LCD. The lower display shows ‘HI’, 
indicating the high-point is being calibrated. Immerse the electrode in 
8.24 mg/l dissolved oxygen solution. Immerse the temperature probe in 
the solution if ATC is enabled. The upper display shows the measured 
value of dissolved oxygen of the solution. Allow the reading to stabilize. 
LCD shows ‘READY’ annunciator when the reading is stable. 
Press  or  key to adjust the reading to 8.24 mg/l. The Transmitter 
allows adjusting up to r50% of the measured value. 
Press ENT key to confirm the reading. 
NOTE: 
The transmitter does not accept the calibration and shows 
calibration error if the displayed (measured) value is more than 
r50% of the high-point value. 
4
The calibration is completed. The DO Transmitter reverts to 
measurement mode. 










