Users Manual Part 1

Device overview

Intended use

The device is a sequencing instrument that measures optical

and electronic signals of the reporting molecules to decode the

sequence information of a piece of DNA or RNA fragment through

the use of instrument specific reagents and flow cells imaging

hardware, and data analysis software. The device is intended for

decoding the sequence of DNAs or RNAs prepared into specific

sequencing libraries such as DNA nanoball libraries. It can be used

for whole genome sequencing and de novo sequencing.

Warning

This device is intended only for scientific research and should not be used

for clinical diagnosis.

Working principle

The device adopts the advanced DNA Nanoball (DNB) and the core

technology of combinatorial probe-anchor synthesis (cPAS) and

uses a regular arrayed flow cell with the special decorated surface.

Each decorated site of the flow cell contains a single DNB, and the

decorated site is evenly arranged on the flow cell, ensuring that the

optical signal of different Nanoball cannot be interrupted by each

other. Therefore, the accuracy of signal process is improved.

The following figure demonstrates how to make DNBs:

Ad-inserted

ss DNA circle

Replicate

DNA Nanoball (DNB)

N×

3×

2×

1×

DNBs

Figure 1 Making DNBs