DOC022.52.
Table of Contents Specifications ..................................................................................................................................................................................5 General information .....................................................................................................................................................................5 Safety information ...........................................................................................
Table of Contents Hach Standard Method procedure ...................................................................................................................................................14 Completion steps for all procedures ................................................................................................................................................15 Determination of results ........................................................................................................
Table of Contents Optional reagents .............................................................................................................................................................................22 Accessories ......................................................................................................................................................................................
Table of Contents 4
Specifications Specifications are subject to change without notice.
Failure to do so could result in serious injury to the operator or damage to the equipment. Make sure that the protection provided by this equipment is not impaired. Do not use or install this equipment in any manner other than that specified in this manual. Use of hazard information DANGER Indicates a potentially or imminently hazardous situation which, if not avoided, will result in death or serious injury.
accordance with the instruction manual, may cause harmful interference to radio communications. Operation of this equipment in a residential area is likely to cause harmful interference, in which case the user will be required to correct the interference at their expense. The following techniques can be used to reduce interference problems: The instrument adjusts for any negative errors produced when heat is applied to a sample.
Note: The use of the specified cable or an equivalent shielded cable is necessary to meet radio frequency emissions requirements. Figure 1 External connections Bottle connections Each bottle position/channel has the applicable tube numbered with a plastic sleeve. The bottle positions are numbered 1 through 6 with number 1 in the back left corner of the chassis. Use the channel selection keys as a guide to the bottle positions Figure 2 on page 8.
Channel selection keys OFF key Push the related channel selection key to show data for one of the six bottles. The channel selection keys are also used in the instrument setup menu to select a parameter to be edited. Refer to Figure 2 on page 8 and Table 3. When a test is in DELAY or RUN modes, push and hold OFF to manually end the test. The instrument will show END. The OFF key is also used to exit the instrument setup menu or range selection menu. The changes made before the menu is exited are saved.
Standard operation Download test results To transfer test results to a PC: 1. Select PROGRAMS>ACCESSORIES>COMMUNICATIONS>HYPERTERM INAL. 2. In the Connection Description window, type in a name for the connection and select an icon to represent the connection. Click OK. 3. In the Connect To window, use the drop-down menu to select the COM port connected to the instrument. Click OK. 4. Configure the COM port properties: BPS = 9600, Data Bits = 8, Parity = None, Stop Bits = 1, Flow Control = None. 5. Click OK.
0.33, 12 0.38, 8 0.44, 10 0.50, 12 0.55, 12 0.61, 14 Test Run to Completion $ CAUTION Chemical exposure hazard. Dispose of chemicals and wastes in accordance with local, regional and national regulations. Procedure notes There are three procedure variations. Select the procedure that meets the application requirements. Print test results The BODTrak II is compatible with the Citizen PD-24 printer, which is available as an optional accessory (Accessories on page 22). 1.
Table 4 Simplified sample volumes (continued) Dilution is necessary if samples have BOD values more than 700 mg/L. At elevations higher than 5000 feet above sea level, the 0 to 35 mg/L BOD range is decreased to 0 to 25 mg/L BOD. Adjustment is not necessary for other test ranges. Refer to Special considerations on page 18 for special considerations including sample seeding and pretreatment. BOD range (mg/L) Sample volume (mL) 0 to 350 160 0 to 700 95 Use only BODTrak II stir bars and bottles.
Hach GGA (glucose/glutamic acid) procedure Items to collect: • • • • • • Note: If the seed strength is unknown, use 20 mL. Adjust the seed volume as necessary to achieve optimum GGA results. Use the same amount of seed for all GGA samples and seed blanks. BODTrak II bottle Graduated cylinder Volumetric pipet and pipet filler ® Tensette pipet and pipet tips Wash water bottle Ampule breaker Required reagents: • Deionized water • Hach GGA solution • Nutrient buffer pillow (1x) 1.
Hach Standard Method procedure Note: If the seed strength is unknown, use 20 mL. Adjust the seed volume as necessary to achieve optimum results. Items to collect: • • • • • • Thermometer BODTrak II bottle Blender (optional) Graduated cylinder Tensette pipet and pipet tips Wash water bottle Required reagents: • 1 nutrient buffer pillow • Additional nutrient or buffer (optional) • Deionized water 1. Heat or cool the sample to 19 to 21 ºC (66 to 70 ºF). 2.
Completion steps for all procedures Items to collect: • • • • • 7. Fill to the final test range volume if necessary with a deionized wash water bottle (Table 6). 8. Move the prepared sample from the graduated cylinder to a BODTrak II bottle. Continue to Completion steps for all procedures on page 15. Note: Repeat steps 1 to 8 for additional samples. 1. Put a stir bar into the bottle. 2. Put a seal cup into the neck of the bottle.
7. Push and hold the LEFT and RIGHT arrows at the same time to go to the instrument setup menu. Note: Set the time and date if necessary (Set the clock on page 9). 8. Push the channel 6 key to go to the test length parameter. Use the arrow keys to select a 5, 7 or 10 day test. Note: The selected test length is for all six channels. 13. Push and hold ON to start a test. A graph will show. Note: To cancel a test, push and hold OFF.
Determination of results After the end of the selected test period (5, 7 or 10 days), the display shows "END". The results are given based on the selected procedure: Simplified, Hach GGA or Hach Standard Method. Simplified sample results The simplified procedure results are shown on the BODTrak II display. Push the applicable channel selection key to show the results. Note: If the sample was pre-diluted, apply a dilution factor to the instrument reading. Refer to Sample dilution on page 18.
When the oxygen requirement of a sample is more than 700 mg/L, dilute the sample with high-quality distilled or deionized water. Calculate the results to include the additional dilution factor. Example: if the BOD of the sample is 1000 mg/L, dilute the sample 1:1 with distilled or deionized water. The estimated BOD is now 500 mg/L. Use the sample volume specified in the table for the 0 to 700 mg/L range of the selected procedure. Multiply the instrument reading result by 2.
Chlorine Chlorine in the sample must be removed prior to testing. Keep the sample at room temperature for 1 to 2 hours before a test to dissipate low chlorine concentrations. If any chlorine remains after sitting for 2 hours, or if the chlorine concentration is high, add sodium thiosulfate to remove the chlorine. 1. In a 250-mL Erlenmeyer flask, add 100 mL of sample. 2. Add 10 mL of 100 g/L potassium iodide solution and 10 mL of 0.02 N sulfuric acid standard solution to the Erlenmeyer flask. 3.
bottle fence. Move the tubing counter-clockwise and secure the bottle cap inside the fence. Figure 4 Incorrect curves Troubleshooting Incorrect BOD curves Figure 4 shows incorrect BOD curves for a 10 day test period. For typical curves, refer to Typical curves on page 17. 1 High oxygen demand 4 Initial sample temperature below 20 ºC or supersaturated with oxygen 2 Nitrification 5 Bottle leak 3 Excessive time lag High oxygen demand Refer to Figure 4 on page 20.
cause results as shown in Curve 1. Dilute the sample or use a higher BOD range and a different sample volume. Refer to the Sample dilution, Simplified procedure, Hach GGA procedure or the Hach Standard method procedure for more information. When the BOD range of a sample is unknown: Bacteria acclimation also causes conditions that can cause curve 3. This sometimes occurs with standards and added seed. Add more seed or select a different seed source.
Required reagents Description Accessories Quantity Item number Respirometric BOD nutrient buffer pillows 1 2962266 Potassium hydroxide pellets 1 31425 Optional reagents Description Nitrification inhibitor Item number Ampule breaker kit for voluette ampules 1 2196800 Bottle, wash, 500 mL 1 62011 Bottle, polyethylene, with spigot, 4 L 1 1486817 Brush, cylinder, size 2 1 68700 1 1405940 Quantity Item no.
Accessories (continued) Description Quantity Item number Pipet serological, glass, 10-mL 1 53238 Printer, Citizen PD-24 with cable 1 2960100 Standard Methods for the Examination of Water and Wastewater 1 2270800 Support stand, buret 1 32900 Thermometer, Mercury, –20 to 110 ºC 1 56601 Thermometer, non-Mercury, –20 to 110 ºC 1 2635702 Water Still, 120 V 1 2615900 Water Still, 220 V 1 2615902 Water System, Ultrapure, Millipore DirectQ3 1 2512100 DQ3 purification pack 1 2512201
24 English
HACH COMPANY World Headquarters P.O. Box 389, Loveland, CO 80539-0389 U.S.A. Tel. (970) 669-3050 (800) 227-4224 (U.S.A. only) Fax (970) 669-2932 orders@hach.com www.hach.com © HACH LANGE GMBH Willstätterstraße 11 D-40549 Düsseldorf, Germany Tel. +49 (0) 2 11 52 88-320 Fax +49 (0) 2 11 52 88-210 info@hach-lange.de www.hach-lange.de HACH LANGE Sàrl 6, route de Compois 1222 Vésenaz SWITZERLAND Tel. +41 22 594 6400 Fax +41 22 594 6499 Hach Company/Hach Lange GmbH, 2013. All rights reserved.
