DOC022.53.90072 BODTrak™ II USER MANUAL 02/2010, Edition 2 © Hach Company, 2008, 2010. All rights reserved. Printed in China.
Table of contents Section 1 Specifications .................................................................................. 5 Section 2 General information ....................................................................... 7 2.1 Safety information ....................................................................................... 7 2.1.1 Use of hazard information ................................................................ 7 2.1.2 Precautionary labels .........................................
Table of contents 6.1.2 Stir bars and seal cups ....................................................................35 6.1.3 Bottle fences ...................................................................................35 Section 7 Troubleshooting ............................................................................37 7.1 High oxygen demand .................................................................................38 7.2 Nitrification ........................................................
Section 1 Specifications Specifications are subject to change without notice. Table 1 Specifications General Range Selectable, 0 to 35, 0 to 70, 0 to 350, 0 to 700 mg/L Dimensions 28.9 x 26 x 9.8 cm (11 3/8 x 10 ¼ x 3 7/8 inches) External power supply Input: 110 to 240 V, 50/60 Hz, Output: 24 V, UL CSA, and TUV approved Capacity Six 492 mL bottles Shipping weight 4 kg (8.
Specifications Table 2 Certification (continued) Radio frequency interference This Class A digital apparatus meets all requirements of the Canadian Interference-Causing Equipment Regulations. This device complies with Part 15 of the FCC Rules. Operation is subject to the following two conditions: (1) This device may not cause harmful interference, and (2) this device must accept any interference received, including interference that may cause undesired operation.
Section 2 General information 2.1 Safety information Please read this entire manual before unpacking, setting up or operating this equipment. Pay attention to all danger and caution statements. Failure to do so could result in serious injury to the operator or damage to the equipment. Make sure that the protection provided by this equipment is not impaired, do not use or install this equipment in any manner other than that specified in this manual. 2.1.
General information 2.2 Theory of operation Respirometric Biochemical Oxygen Demand (BOD) is a test done at 20 °C (68 °F) in a controlled environment. The test period can be 5, 7 or 10 days, contingent on the analysis or protocol. The BOD test measures the quantity of oxygen consumed by bacteria that oxidize organic matter in a water sample. The test is used to measure waste loadings at wastewater treatment plants and to examine the efficiency of wastewater treatment.
General information 2.2.2 Pressure sensor function The BODTrak II is sealed to prevent external atmospheric pressure changes in the test bottle. Pressure sensors monitor air pressure in the sample bottles. When oxygen is consumed, the pressure in the bottle head space drops. The pressure drop correlates directly to BOD. 2.2.3 Removing carbon dioxide Carbon dioxide is made when microorganisms oxidize organic matter in the sample.
Section 3 Installation 3.1 Component list Compare each item below to the items in the shipment. If an item is missing or damaged, refer to the manufacturer.
Installation 3.2 Electrical installation The power adaptor supplies AC power to the IEC universal connector (Figure 1). The power switch powers the instrument on and off.
Section 4 Operation 4.1 Operational controls The BODTrak™ II operator controls are shown in Figure 2. Figure 2 Operational controls 1 Display 4 ON/OFF keys1 2 Channel selection keys 5 Power indicator 3 Arrow keys 1 The ON/OFF keys start and stop a test. They do not power the instrument on and off.
Operation 4.1.1 Channel selection keys Push the related channel selection key to show data for one of the 6 bottles. The channel selection keys are also used in the instrument setup menu to choose a parameter to be edited (Table 3). Table 3 Channel key setup parameters 4.1.
Operation 4.3 Setting the clock All channels must show END or CLEAR before the clock can be set. Push and hold the two arrow keys at the same time until the instrument setup menu is shown. Select the clock parameter to be adjusted by pushing the applicable channel key (Table 3 on page 14). Use the arrow keys to edit the chosen parameter. Adjust each parameter in the same manner. When all time adjustments are complete, push the OFF key to save and go back to the data display screen. 4.
Operation 4.5 Downloading test results To transfer test results to a PC: 1. Choose PROGRAMS, ACCESSORIES, COMMUNICATIONS, HYPERTERMINAL. 2. In the Connection Description window, type in a name for the connection and choose an icon to represent it. Click OK. 3. In the Connect To window, use the drop-down menu to choose the COM port connected to the BODTrak II instrument. Click OK. 4. Configure the COM port properties: BPS = 9600, Data Bits = 8, Parity = None, Stop Bits = 1, Flow Control = None. 5.
Operation 4.5.2 Data format When a result array is downloaded to HyperTerminal, all data from the test are sent without pause. The data flow cannot be stopped or paused. Figure 3 shows channel number, start date, start time, and the format of the downloaded data. BOD values in mg/L follow. Only the first data points, of a maximum of 360 equal distance points, are shown in this example. Each line ends with a carriage return and a line feed.
Operation 4.5.3 Printing test results The BODTrak II is compatible with the Citizen PD-24 printer, which is available as an optional accessory (Section 8 on page 41). Connect the printer cable to the serial port on the BODTrak II using the gender adapter provided with the printer. Make sure the printer interface settings are correct (section 4.4 on page 15). Power on the BODTrak II instrument. Push and hold the applicable channel number for approximately 5 seconds at any time during a test.
Section 5 BODTrak™ II procedures 5.1 General Information There are three BODTrak II procedure variations. Choose the procedure that meets the application requirements. The Simplified procedure (section 5.2 on page 20) is recommended when sample seeding, extra nutrients or buffers are not necessary. It is also recommended when accuracy requirements are not stringent. The Hach GGA (glucose/glutamic acid) procedure (section 5.
BODTrak™ II procedures 5.2 Simplified procedure Required apparatus: BODTrak II bottle Thermometer Blender (optional) Graduated cylinder Required reagents: 1 nutrient buffer pillow Table 4 Simplified sample volumes BOD range mg/L Sample volume mL 0 to 35 420 0 to 70 355 0 to 350 160 0 to 700 95 1. Heat or cool the sample to 19 to 21 ºC (66 F to 70 ºF). 20 2. Homogenize the sample in a blender if it contains large settleable or floatable solids. 3.
BODTrak™ II procedures 5. Transfer the contents of 4. Add the contents of 1 nutrient buffer pillow to the the graduated cylinder to a BODTrak II bottle. graduated cylinder. Repeat steps 1 to 5 for additional samples. 6. Continue to the completion steps for all procedures (section 5.5 on page 27).
BODTrak™ II procedures 5.3 Hach GGA (glucose/glutamic acid) procedure Required apparatus: BODTrak II bottle Graduated cylinder Volumetric pipet and pipet filler Tensette® pipet and pipet tips Wash water bottle Ampule breaker Required reagents: Deionized water Hach GGA solution 1 nutrient buffer pillow Before starting the test: Use Hach BOD Standard Solution Ampules for Manometric Method (3000 mg/L Glucose, 3000 mg/L Glutamic acid).
BODTrak™ II procedures Table 5 GGA sample volumes BOD range (mg/L) GGA volume (mL) Seed volume (mL) Final Volume (mL) 0 to 350 8.0 10 to 35 160 Note: If seed strength is unknown, use 20 mL. Adjust seed volume as necessary to achieve optimum GGA results. Use the same amount of seed for all GGA samples and seed blank. 1. Add approximately 30 mL of deionized water to a 200 mL graduated cylinder. 2. Use a volumetric pipet 3. Add the contents of 1 to transfer 8.
BODTrak™ II procedures 5.4 Hach Standard Method procedure Required apparatus: Thermometer BODTrak II bottle Blender (optional) Graduated cylinder Tensette pipet and pipet tips Wash water bottle Required reagents: 1 nutrient buffer pillow Additional nutrient or buffer (optional) Deionized water Before starting the test: Use the sample volume table to choose the correct sample size (Table 6). If seeding samples, prepare a seed blank before preparing a sample.
BODTrak™ II procedures 1. Heat or cool the sample to 19 to 21 ºC (66 to 70 ºF). 2. Homogenize the sample in a blender if it contains large settleable or floatable solids. 4. Add the contents of 1 5. If seeding the sample, nutrient buffer pillow to the use a tensette pipet to add graduated cylinder. the correct quantity of seed to the graduated cylinder (Table 6 on page 24). 3. Choose the correct sample size for the sample range (Table 6 on page 24). Measure the sample into a graduated cylinder. 6.
BODTrak™ II procedures 7. Fill to the final test range volume, if necessary, with a deionized wash water bottle (Table 6 on page 24). 26 8. Transfer the prepared sample from the graduated cylinder to a BODTrak II bottle. Note: Repeat steps 1 to 8 for additional samples. 9. Continue to the completion steps for all procedures (section 5.5 on page 27).
BODTrak™ II procedures 5.5 Completion steps for all procedures Required apparatus: BODTrak II Spatula scoop BOD incubator Seal cup Stir bar Required reagents: 2 potassium hydroxide pellets 1. Put a BODTrak II stir bar into the bottle. 2. Put a seal cup into the neck of the bottle. 3. Use a spatula scoop to add 2 potassium hydroxide pellets to the seal cup. Repeat steps 1 to 3 for each sample bottle.
BODTrak™ II procedures 4. Put the bottles on the BODTrak II chassis. Connect the applicable tube to the sample bottle and tighten the cap. 5. Put the instrument in the incubator. The incubator temperature must be 20 ± 1 ºC (68 ± 1 ºF). Note: Instrument performance has not been tested at other temperatures. 7. Push and hold the left and right arrow keys at the same time to access the instrument setup menu. 8. Push the Channel 6 key to access the test length parameter.
BODTrak™ II procedures 10.To start the test, push the channel number applicable to the bottle. 11.Push the ON key. The range selection menu is shown. 13.Push and hold the ON key to start a test. A graph will be displayed. 14.Do steps 10 through 13 again to set the test range and start each of the 6 channels. It is not necessary to operate all 6 channels if less than 6 samples are available. Note: To cancel a test push and hold the OFF key.
BODTrak™ II procedures 5.5.1 Determination of results After the end of the chosen test period (5, 7 or 10 days), END is shown on the display. The procedure that is done dictates the determination of the results. The results are determined based on the selected procedure: Simplified, Hach GGA or Hach Standard Method. 5.5.1.1 Simplified sample results The Simplified Procedure results are shown on the BODTrak II display. Push the applicable channel selection key to show the results.
BODTrak™ II procedures 4.
BODTrak™ II procedures 5.6 Typical curves Typical curves through a 10 day test period are shown in Figure 4. For incorrect curves refer to Figure 5 on page 37.
BODTrak™ II procedures 5.7 Special considerations 5.7.1 Sample dilution Unknown sample BOD effluent is typically in the 0 to 70 mg/L range. Unknown sample BOD influent is typically in the 0 to 700 mg/L range. When the oxygen requirement of a sample is more than 700 mg/L, dilute the sample with high-quality distilled or deionized water. Calculate the results to include the additional dilution factor. Example: If the BOD of the sample is 1000 mg/L, dilute the sample 1:1 with distilled or deionized water.
BODTrak™ II procedures 5.7.5 Chlorine Any chlorine in the sample must be removed prior to testing. Keep the sample at room temperature for 1 to 2 hours before a test to dissipate low chlorine concentrations. If any chlorine remains after sitting for 2 hours or if the chlorine concentration is high, add sodium thiosulfate to remove the chlorine: 1. In a 250 mL erlenmeyer flask add 100-mL of sample. 2. Add 10 mL of 100 g/L potassium iodide solution and 10 mL of 0.
Section 6 Maintenance DANGER Only qualified personnel should conduct the tasks described in this section of the manual. 6.1 Cleaning the instrument Clean spills on the BODTrak™ II instrument with a soft cloth which has been dampened with deionized or distilled water. 6.1.1 Sample bottles After each test, empty the sample bottles and flush them thoroughly with hot water. Use a brush, hot water and soap to remove residue. Residue creates a BOD.
Section 7 Troubleshooting Incorrect BOD curves through a 10 day test period are shown in Figure 5. For typical curves refer to Figure 4 on page 32.
Troubleshooting 7.1 High oxygen demand Samples that are above range (for example, a BOD over 350 mg/L when a 160-mL sample is taken) will cause results as shown in Curve 1 (Figure 5 on page 37). Dilute the sample (section 5.7 on page 33) or use a higher BOD range and a different sample volume (Table 4 on page 20, Table 5 on page 23 or Table 6 on page 24). When the BOD range of a sample is unknown: • Use the results from the Chemical Oxygen Demand (COD) test.
Troubleshooting 7.5 Bottle leak Curve 5 (Figure 5 on page 37) shows a bottle leak. A bottle leak can also cause no response from the system. If such a response occurs, check the seal cup and bottle cap for contamination or damage.
Section 8 Replacement parts and accessories 8.1 Replacement parts Description Quantity Item Number BODTrak™ II Instrument, 115/230 VAC 1 2952400 Bottle, BODTrak II, amber (6x) 1 714421 Power cord, 18/3 SVT 7.
8.3 Optional reagents (continued) Description Quantity Item Number Sulfuric acid, 0.02 N standard solution, 1000 mL 1 20353 Sulfuric acid, 1.0 N standard solution, 1000 mL 1 127053 Voluette ampule standard for BOD, 3000 mg/L for manometric, 10-mL/ampule, 16 ampules 1 1486610 Quantity Item Number 1 2196800 8.
Replacement parts and accessories 8.
Section 9 Contact Information HACH Company World Headquarters Repair Service in the United States: Repair Service in Canada: P.O. Box 389 Loveland, Colorado 80539-0389 U.S.A. Tel (800) 227-HACH (800) 227-4224 (U.S.A. only) Fax (970) 669-2932 orders@hach.com www.hach.com HACH Company Ames Service 100 Dayton Avenue Ames, Iowa 50010 Tel (800) 227-4224 (U.S.A. only) Fax (515) 232-3835 Hach Sales & Service Canada Ltd.
Contact Information HACH LANGE S.L.U. HACH LANGE LDA HACH LANGE SP.ZO.O. Edif. Arteaga Centrum C/Larrauri, 1C- 2ª Pl. E-48160 Derio/Vizcaya Tel. +34 94 657 33 88 Fax +34 94 657 33 97 info@hach-lange.es www.hach-lange.es Av. do Forte nº8 Fracção M P-2790-072 Carnaxide Tel. +351 214 253 420 Fax +351 214 253 429 info@hach-lange.pt www.hach-lange.pt ul. Opolska 143 a PL-52-013 Wrocław Tel. +48 (0)71 342 10-83 Fax +48 (0)71 342 10-79 info@hach-lange.pl www.hach-lange.pl HACH LANGE S.R.O. HACH LANGE KFT.
