DOC022.53.00720 DR 2800 USER MANUAL January 2008, Edition 2 © HACH Company, 2007–2008. All rights reserved. Printed in Germany.
Table of Contents Section 1 Specifications ........................................................................................................................................ 7 Section 2 General Information .............................................................................................................................. 9 2.1 Safety information .............................................................................................................................................. 9 2.
Table of Contents 5.3 Store, recall, send and delete data .................................................................................................................. 37 5.3.1 The data log ............................................................................................................................................ 37 5.3.1.1 Auto/manual data storage ........................................................................................................... 37 5.3.1.
Table of Contents 6.2 Favorite Programs ........................................................................................................................................... 81 6.2.1 Recall a favorite program ........................................................................................................................ 81 6.2.2 Delete a favorite program ....................................................................................................................... 81 6.
Table of Contents 6
Section 1 Specifications Specifications are subject to change without notice. Performance Specifications Operating Mode Transmittance (%), Absorbance and Concentration Source Lamp Gas-filled Tungsten (visible) Wavelength Range 340–900 nm Wavelength Accuracy ± 1.5 nm Wavelength Reproducibility < 0.1 nm Wavelength Resolution 1 nm Wavelength Calibration Automatic Wavelength Selection Automatic, based on method selection Spectral Bandwidth < 8 nm Photometric measuring range ± 3.
Specifications 8
Section 2 General Information 2.1 Safety information Please read this entire manual before unpacking, setting up or operating this equipment. Pay attention to all danger, warning and caution statements. Failure to do so could result in serious injury to the operator or damage to the equipment. Make sure that the protection provided by this equipment is not impaired, do not use or install this equipment in any manner other than that specified in this manual. 2.1.
General Information Permissible Exposure value. Therefore, for Class 1 LASERS the output power is below the level at which it is believed eye damage will occur. Exposure to the beam of a Class 1 LASER will not result in eye injury. Class 1 LASERS may therefore be considered safe. However, Class 1 LASER products may contain LASER systems of a higher Class but there are adequate engineering control measures to ensure that access to the beam is not reasonably likely.
Section 3 Installation WARNING Electrical and Fire Hazards. Use only the provided power supply. Only qualified personnel should conduct the tasks described in this section of the manual. 3.
Installation 3.3 Power connections Install the correct adapter plug on the supplied external power supply (Figure 1) by sliding the adapter on until it "clicks" into position. Correctly mounted, both housing of power supply and plug are in line. Plug the external power supply cord into the connector on the back panel of the instrument, then plug the supply into a power outlet (100–240 V~ / 50–60 Hz). Press the power switch on the back of the instrument to initialize power (Figure 2 on page 13).
Installation 3.4 Connection The DR 2800 has two USB interfaces as a standard feature, located on the back of the instrument (Figure 2). The USB Type A interface is used for communications with a printer, USB memory stick, barcode scanner, or keyboard. A USB memory stick is used to update instrument software. The USB Type B interface is used for communications with a PC. The optional Hach Data Trans software (see section 5.2.6.3 on page 33) must be installed on the PC for this use.
Installation 3.5 Cell compartments, Cell adapters, Light Shield and Protective Cover 3.5.1 Cell compartments and adapters The DR 2800 has two cell compartments (Figure 3). Only one cuvette/sample cell type at a time can be used for a measurement. Cell compartment #1 • 13-mm and 16-mm round cuvettes/vials Note: Cell compartment #1 contains a barcode reader for cuvettes/vials. Cell compartment #2 Cell compartment #2 uses adapters to accommodate different cuvette/sample cell types.
Installation Figure 4 Cuvette/Sample cell adapters 1 10 mm square cuvette/sample cell adapter (A) 2 1 inch Pour-Thru adapter (B) 3 1 inch round cuvette/sample cell adapter (C) 3.5.2 Installation of the cuvette/sample cell adapters 1. Open the cell compartment. 2. Select the correct adapter for the cuvette/sample cell type. 3. Insert the adapter so the arrow on top of the adapter points to the left (Figure 5 on page 16) and the orientation tab fits the groove in the compartment opening.
Installation Figure 5 Installation of a cuvette/sample cell adapter 3.5.3 Use of the light shield for measurements The light shield (Figure 6 on page 17) prevents light interference when using 13 mm and 16 mm vial tests and must be in place before measurements can be taken in cell compartment #1. The light shield is required only when using 13 mm or 16 mm vial tests.
Installation The DR 2800 is shipped with the light shield installed. Remove the light shield before using cell compartment #2. The light shield can be stored in the Protective Cover (Figure 8 on page 18) Figure 6 Light Shield Installation of the light shield 1. Open the cell compartment. 2. Insert the light shield so the arrow on the light shield points to the left and the orientation tab fits the groove in the compartment opening (Figure 7).
Installation 3.5.4 Protective Cover Figure 8 Protective Cover 1 Protective Cover 2 Protective Cover (inside view) The inside of the Protective Cover is intended to house the cuvette/sample cell adapters. The recesses for holding the cuvette/sample cell adapters are marked with the corresponding letters. 18 3 Protective Cover with inserted cuvette/sample cell adapter A in position A.
Installation 3.6 Mobile use of the DR 2800 for field analysis If the DR 2800 spectrophotometer is used to carry out field analyzes, it may be necessary to take a number of measures to compensate for the fluctuating ambient light conditions.
Installation 3.6.1 Position the Protective Cover Figure 9 Position the Protective Cover 1. After you have inserted the zero solution cell or the sample cell, position the Protective Cover over the open cell compartment. The lettering "Protective Cover" must be readable from the display side of the instrument. 2. Press the Protective Cover lightly into the correct position until the cell compartment is completely sealed and the Protective Cover cannot slip off. 3. Carry out the measurement.
Installation 3.
Installation 22
Section 4 Start Up 4.1 Power the instrument on and off 1. Plug in to the power supply (laboratory analysis) or insert the battery (field analysis). 2. The push-button switch on the back of the instrument switches the instrument on (press for about 1 second) and off (press for about 3 to 5 seconds). An acoustic signal confirms that the instrument has been switched off. Note: Do not turn the instrument off and on in rapid succession.
Start Up If the instrument detects any deviation relative to the last calibration, a system check has to be carried out. 4. Remove the cuvette from the cell compartment and close the lid. 5. Then press Start. The system check is carried out (duration approx. 6 minutes). Note: Further error messages during self check, see Section 8 on page 117.
Section 5 Standard Operations 5.1 Overview 5.1.1 Tips for the use of the touch screen The entire screen is touch-activated. To make a selection, press the screen with a fingernail, fingertip, pencil eraser or a stylus. Do not press the screen with a sharp object, such as the tip of a ball point pen. • Do not place anything on top of the screen, to prevent damage or scratching on the screen. • Press keys, words or icons to select them. • Use scroll bars to move up and down long lists very quickly.
Standard Operations 5.1.3 Main Menu A variety of modes may be selected from the ”Main Menu“. The following table briefly describes each menu option. Table 2 ”Main Menu“ Options DR 2800 Option Function Stored Programs / Barcode Programs (HACH LANGE Programs) Stored programs are pre-programmed methods that make use of HACH reagents and LANGE cuvette tests and pipette tests. The DR 2800 Procedures Manual contains illustrated, step-by-step procedures for analyzes using HACH programs.
Standard Operations 5.2 Instrument Setup mode 1. Select Instrument Setup in the ”Main Menu“. A selection of functions appears in order to configure the functions of the instrument. 5.2.1 Operator ID Use this option to enter up to 30 sets of operator initials (up to 5 characters each) into the instrument. This feature helps record which operator measured each sample. 1. Press Operator ID in the Instrument Setup. 2. Press New to enter a new Operator ID. 3.
Standard Operations 5.2.2 Sample ID Use this option to enter up to 100 Sample Identification tags (up to 13 characters each) into the instrument. Sample IDs can be used to specify the sample location or other sample specific information. 1. Press Sample ID in the Instrument Setup. 2. Press New to enter a new Sample ID. 3. Use the alphanumeric keypad to enter a new Sample ID. Note: If a USB Barcode handset scanner ( see Section 9 on page 119) is connected, Sample IDs can also be scanned.
Standard Operations 5.2.3 Date and time 1. Press Date & Time in the Instrument Setup. 2. The date and time are subdivided over a number of fields. Press the appropriate field and use the arrow keys to change the value. 3. Press OK to confirm. The instrument will return to Instrument Setup. 5.2.4 Display and sound preferences 1. Press Display & Sound in the Instrument Setup. The following options will be displayed: • Display Contrast—Adjusts the display brightness to suit lighting conditions.
Standard Operations 2. Select one of the options under Standby mode to set the length of the idle period that can elapse before the instrument switches to the Standby mode when running on battery power. Note: In standby mode, the backlighting of the screen is switched off. Touching the display causes the lighting to switch on again. 3.
Standard Operations 5.2.6 PC and printer The instrument is provided with 2 USB interfaces, which are located on the back of the instrument (see Figure 2 on page 13). These interfaces can be used for exporting data and graphics to a printer, updating data and for data communication to a personal computer. These interfaces can be used for the connection of a USB stick, an external USB keyboard or a USB Barcode handset scanner. Note: A USB hub may be used to connect several accessories at a time.
Standard Operations Printer Setup: • Resolution: Print quality • Paper: Paper size Note: If an optional Thermal Printer is connected, the function "Auto Send" on/off is available. 4. Select Auto-Send: On to send all measured data automatically to the Thermal printer. Note: The option Auto-Send is not available for any other printer (e.g. ink jet printer). 5. Press Resolution to select the print quality. Select between • 100 dpi • 150 dpi and • 300 dpi 6. Press OK to confirm.
Standard Operations 7. Press Paper to select the paper size. Select between • Letter • Legal • Executive • A4 8. Press OK to confirm. Note: Press OK again to return to the PC & Printer menu. 5.2.6.2 Print data 1. Press Recall Data in the Main Menu. 2. Select the data source, where the data to be printed are stored. 3. A list is displayed. Data can be filtered. For more information see section 5.3.1.2 on page 37. 4.
Standard Operations 4. Select Auto-Send: On to send all measured data automatically to the PC. Note: If Auto-Send: Off is selected, the PC & Printer icon must be pressed, in order to send data to the PC. Note: The remote function is only for monitoring the data transfer. 5.2.7 Password The Password menu contains a variety of security settings to control access to various functions. For example, prevent unauthorized changes to stored programs or instrument configurations. 1.
Standard Operations 4. Press Security List to lock various functions for unauthorized users. 5. Highlight the desired functions to control. 6. Confirm the Security List with OK to return to the Password menu. 7. Press On to highlight the new settings of the Security List. 8. Enter the new Password again to confirm. 9. Press OK to return to Instrument Setup. Note: The alphanumeric keypad to the Password inquiry appears when a user tries to reach a locked setting. 5.2.7.1 Password deactivation 1.
Standard Operations 3. Press Off to deactivate the settings of the Security List. 4. Press OK to return to Instrument Setup. Note: Use this function to delete the former Password or to enter a new one.
Standard Operations 5.3 Store, recall, send and delete data 5.3.1 The data log The Data Log will store up to 500 readings taken in the modes: Stored Programs, Barcode Programs, User Programs, Favorite Programs, Single Wavelength and Multi Wavelength. A complete record of the analysis is stored, including the Date, Time, Results, Sample ID, and Operator ID. 5.3.1.
Standard Operations 4. The function Filter Settings is used to search for specific items. 5. Highlight On to turn on the filters to select data by • Sample ID • Operator ID • Start Date • Parameter or any combination of the four. 6. Press OK to confirm the selection. The chosen items are listed. 7. Press View Details to get more information. 5.3.1.3 Send data from the data log Data is sent from the data log as CSV (Comma Separated Value) files through a USB memory stick to a file named DATALOG.
Standard Operations 5.3.1.4 Delete stored data from the data log 1. Press Recall Data in the Main Menu. 2. Press Data Log>Options>Delete. 3. Highlight Single Point or Filtered data or All data and press OK to confirm. Note: The number in parentheses is the total number of data sets assigned to this selection.
Standard Operations 5.3.2 Time Course The instrument can store 20 Time Course Data sets. The data can be stored manually at the user's discretion after viewing the data. 5.3.2.1 Data Storage from Time Course 1. Press the Store icon in the ”Options“ menu from Time Course mode after a reading is taken. The Store Data list opens. 2. Press Store to save the current scan to the highlighted numbered line. A run can also be overwritten. 5.3.2.2 Recall Stored Data from Time Course 1.
Standard Operations 2. Press Graph to look at details. Note: Press View Summary to return to the Recall Data list. 3. Press Table to look at details. Note: Press View Summary to return to the Recall Data list. 5.3.2.3 Send Data from Time Course Option 1: 4. Press Recall Data in the ”Main Menu“ and then Time Course to send the data to a USB memory stick or to a printer. The files will be automatically send as CSV files (Comma Separated Value) to a file ”TCData“ (Time Course Data).
Standard Operations Option 2: 5. Press Time Course and then Options, More... and Send Data to send the data to a USB memory stick or to a printer. The files will be automatically send as CSV files (Comma Separated Value) to a file ”TCData“ (Time Course Data). The file name will be formatted as: ”TCYear_Month_Day_Hour_Minute_Second.CSV“ (Time Course Data) For further processing use a spreadsheet program. 5.3.2.4 Delete Stored Data from Time Course 1.
Standard Operations 5.4 Stored Programs The instrument contains more than 200 programmed procedures. They can be accessed through the Stored Programs menu. 5.4.1 Select a saved test/method; enter user-specific basic data 1. Press Stored Programs in the Main Menu to view an alphabetical list of stored programs with program numbers. The Stored Programs list will appear. 2. Highlight the required test.
Standard Operations Table 4 Stored programs options (continued) Options Description Store Off/On With the Store On setting, all measurement data are stored automatically. With the Store Off setting, no measurement data are stored. % Trans/Conc/Abs To switch to % transmittance, concentration or absorbance readings Send Data icon / Send Data To send Data to a printer, computer or USB memory stick (Type A) Timer icon This functions as a stopwatch.
Standard Operations 5.4.3 Use of program timers Some procedures do not require the use of timers. Other procedures require several timers. These timers are pre-programmed into each Stored Program, along with a description of the activity to be performed during the timed period. 1. Press the Timer icon on the display. 2. Press OK to start the first timer. The timer will count down on the screen. 3. To start the next timed activity for the Stored Program, press the Timer icon and OK.
Standard Operations 5.4.4 Set the dilution factor The Dilution Factor function is used to calculate the original concentration of a sample that has been diluted by a known ratio. For example, dilution factor can bring the analyzed concentration within the test range. 1. Press Options>More…>Dilution Factor. The number entered at the dilution factor prompt will be multiplied by the result to compensate for the adjustment. For example, if the sample has been diluted by a factor of 2, enter 2.
Standard Operations Read a standard before setting Standard Adjust to On. 1. Follow the entire procedure, using a known standard for the sample. 2. After reading the concentration, press Options>More>Standard Adjust. 3. If Standard Adjust is set to Off, turn it On. The Current Reading will show the concentration. The box on the right will show the default standard value for the test, as mentioned in the procedure. 4.
Standard Operations 1. Press Options>More>Chemical Form. 2. Select the Chemical Form. Note: The stoichiometric conversion of the measurement result is carried out automatically. Note: The selected Chemical Form will appear on the display. Test results will be calculated and displayed in this chemical form. 5.4.6.1 Change of the default setting of the chemical form 1. Insert the sample cuvette or blank (depending on the working procedure) into the cell compartment. 2.
Standard Operations 4. Press Options>More>Reagent Blank. 5. Press On to highlight the Reagent Blank function. 6. The concentration shown on the key is the measured value of the reagent blank. To use this value for more analyses of this parameter, press OK. 7. To enter a previously recorded reagent blank value, press the key and use the alphanumeric keypad. 8. Press OK. Note: The Reagent Blank function is deactivated when the measurement program is left.
Standard Operations 4. Remove zero solution and insert sample cuvette/cell into the cell compartment. 5. Press Read. The result will be displayed. 6. For data storage, see section 5.3.1 on page 37. 5.4.9 Add stored programs to the favorite programs list The Favorites menu simplifies test selection by creating a list of the most frequently used tests from the Stored Programs and User Programs. 1. Press Stored Programs in the Main Menu. The Stored Programs list will appear. 2.
Standard Operations 5.5 Barcode Programs A special barcode reader in cell compartment #1 automatically reads the barcode on the 13 mm cuvette/vial as the cuvette/vial completes a single rotation. The instrument uses the barcode identification to automatically set the correct wavelength for the analysis and calculates the result immediately with the help of the stored factors. In addition, measured values are recorded at 10 different positions during a rotation.
Standard Operations • Otherwise, press Barcode Programs in the Main Menu. and insert the blank or sample cuvette/vial (depending on the working procedure) in cell compartment #1. The measurement is started automatically and the results are displayed. To evaluate other cuvette/vial tests and other parameters, insert the prepared cuvette/vial into the cell compartment and read the result. 5.5.2 Select the measuring range Some tests can be used for different measuring ranges.
Standard Operations 5.5.3.1 Change of the default setting of the chemical form 1. Insert the blank or sample cuvette/cell (depending on the working procedure) into the cell compartment. 2. In the result display, press Options>More>Chemical Form. 3. A list of available evaluation forms appear. Select the new default setting. 4. Press Save as Default. The current result and all further measurements will be displayed in the new chemical form. 5.5.
Standard Operations Table 5 Barcode program options (continued) Options Description Save as User Program To store the selected parameters as a User Program, see section 6.1 on page 61. Recall Data Call up saved measurement data or time courses, see section 5.3 on page 37. Instrument Setup Basic data of the instrument, see section 5.2 on page 27. 5.5.5 Sample blank Turbidity and color in the sample matrix can falsify the results of a photometric analysis.
Standard Operations 5.5.6 Update/edit barcode tests 5.5.6.1 Manual update of a barcode test Using the data provided in the barcode, the instrument automatically sets the measurement wavelength and factors. If a discrepancy is detected between the barcode data and the stored data or a new test is identified, the instrument requests an update. Data types are described in Table 6.
Standard Operations Enter the Version 1. Highlight the line containing Version and press Edit. Use the alphanumeric keypad to enter the name indicated in the working procedure. 2. Press OK to confirm. Enter the Measurement Process For detailed information on entering the measurement process, see section 6.1.2 on page 72. Check the working procedure in advance to determine whether this point needs to be changed. 1. Highlight the Measurement Process line in the data overview and press Edit. 2.
Standard Operations Enter the Variables (Factors, Wavelength and Coversion Factors) For detailed information on entering the Variables, see section 6.1.2 on page 72. Check the working procedure in advance to determine whether this point needs to be changed. 1. Highlight the Variables line in the data overview and press Edit. 2. Select the variable, press Edit and use the alphanumeric keypad to enter the data specified in the working procedure (for F1, F2, λ1, U1 etc.). Confirm each entry with OK.
Standard Operations 5.5.6.2 Update an existing barcode test Note: Only in very few cases does the revision of a test require all test specifications to be updated. Option 1: Manual update of test data 1. Insert the light shield in cell compartment #2. 2. The instrument needs to be at the Main Menu or press Barcode Programs in the Main Menu. 3. Insert the blank or sample cuvette/vial (depending on the working procedure) in cell compartment #1. 4.
Standard Operations 5.5.6.3 Program a new test 1. The instrument needs to be at the Main Menu or press Barcode Programs in the Main Menu. 2. Insert the sample cuvette/vial or blank (depending on the working procedure) into the cell compartment. 3. After "Program not available", a data overview is displayed, including the specifications of the test that is to be revised.
Standard Operations 6. Press OK to return to the System Checks menu. Note: When the instrument software has been updated, a prompt to restart the instrument is displayed.
Section 6 Advanced Operations 6.1 User Programs User programs provide the opportunity to complete "made to measure" analysis. The User Programs database is empty when the instrument leaves the factory and is used to accommodate programs created by users specific needs. Here are a few examples of entries: • Programming of user-created procedures. The analysis procedure must be developed first, before it can be programmed.
Advanced Operations Program Number: Specific test number, with which the program can subsequently be called up from the selection list in the User Programs menu or the Favorites menu. 2. Use the alphanumeric keypad to enter a program number between 950 and 999. The lowest available number appears automatically. 3. Press OK. Note: If the program number is already assigned to another user program, a message appears, asking whether the existing program should be replaced.
Advanced Operations 6.1.1.1 Single wavelength settings If the Single Wavelength mode is selected, the following parameters can be defined: Units: Select the required unit from the list and press Next. Note: Units of measure not included in this list can be added in the edit program under Program Options, Edit. Select Units, Edit and then New. Wavelength (single wavelength program type): Use the alphanumeric keypad to enter the measurement wavelength.
Advanced Operations Chemical form: Enter the chemical formula used in the display to represent the analysis parameter. Use the alphanumeric keypad to enter the chemical form and press Next to enter Calibration settings. 6.1.1.2 Multi wavelength settings If the Multi Wavelength mode is selected, the following parameters can be defined: Units: Select the required unit from the list and press Next. Note: Units of measure not included in this list can be added in the edit program under Program Options, Edit.
Advanced Operations List of available absorbance formulas A1 is the absorbance at wavelength 1, A2 is the absorbance at wavelength 2 and so on K1 is the factor at wavelength 1, K2 is the factor at wavelength 2 and so on If a subtraction has to be completed, the factors can be entered with a minus sign. Wavelength λx: Press a λx key and use the alphanumeric keypad to enter a wavelength. Press another λx key and enter the next wavelength.
Advanced Operations Chemical form: Enter the chemical formula used in the display to represent the analysis parameter. Use the alphanumeric keypad to enter the chemical form and press Next to enter Calibration settings. 6.1.1.3 Calibration settings for single and multi wavelength mode A method is calibrated by determining the absorbance values of several standard solutions of known concentration. There are three ways to create and store a calibration curve. Instructions for each method follows (Table 9).
Advanced Operations 2. To enter the standard concentrations and corresponding absorbance values in the displayed table, press the "+" symbol. Use the alphanumeric keypad to enter the values. Press OK and enter the corresponding absorbance value. Press OK. The entered data are displayed in the table. Repeat the sequence for each data point to enter it. 3. To change a value in the table, highlight the appropriate line, press the unit key (e.g.
Advanced Operations Calibration by reading standards 1. Press Read Standards and press Next. 2. To enter the standard concentrations in the displayed table, press the "+" symbol. Use the alphanumeric keypad to enter the standard concentration. Press OK. 3. Press the "+" symbol again (see arrow) and enter the next standard concentration. Repeat this sequence until all standard conentrations (maximum of 24 solutions) have been entered. 4.
Advanced Operations 9. Press Force 0 to change the setting from Off to On. The curve then passes through the origin of the coordinate system. Note: This may have an adverse effect on the correlation coefficient (r2). 10. Press Table to display the table again. 11. When the table has been completed and the curve type has been chosen, press Done when the graph is displayed or Exit when the table is displayed. Go to section 6.1.1.4 on page 70. Calibration by entering the formula 1.
Advanced Operations 6.1.1.4 Store a user program The input of the basic data is complete. An overview of the variable program data is displayed. 1. To enter more specifications or change existing ones, highlight the appropriate line and press Edit. 2. Select Store to save the user program. 3. Press the PC & Printer icon to send the program data to a printer or to a USB memory stick (connect the USB memory stick to the USB interface first).
Advanced Operations Timer 1 / Timer 2 / Timer 3 / Timer 4: This function can define time intervals for up to four timers. Timer designations such as Shake, Wait and Swirl can be assigned. 1. Highlight the appropriate line in the overview of the program data and press Edit. 2. The timers are activated or deactivated with the check boxes in the left part of the display. In the next column, a selection can be made from a list of names that designate the corresponding work step.
Advanced Operations 6.1.2 Free programming program type Free Programming is an advanced option for entering original user-developed methods. When the Free Programming option is selected, an overview of the specifications of the programmed test is displayed. Each input option can be modified to develop the user method. Refer to Table 10 for more information. To modify an input option, select the appropriate line and press Edit. Important Note: Perform steps 1–6 of Programming a New User Method (section 6.1.
Advanced Operations • Should individual program sequences be repeated? The elements of a measuring sequence, such as zero and sample measurements and the timer(s) (reaction times, waiting times, etc.) are individually defined. 6.1.2.2 Enter a new element of a measuring sequence Important Note: Each component of the measurement process must be entered in the order in which it will be completed. 1. Highlight the Measurement Process line in the data overview and press Edit. 2.
Advanced Operations Process Timer key 1. Press the Process Timer key to enter any waiting, reaction or handling times that have to be taken into account. Use the alphanumeric keypad to enter the time. Press OK and confirm the input by pressing OK again. Note: This time is integrated into the measurement process. Note: The entered measurement sequence is displayed. [R] key / Reading... 1. Press the [R] key to program a measurement of the substance that is to be analyzed. Confirm with OK. 2.
Advanced Operations Deleting an element of a measuring sequence Select the appropriate line and press Delete. The element is deleted. Inserting an element of a measuring sequence Select the line in the measuring sequence where the insertion is be made and press New. A new element can be entered at the selected position. When the input is complete, press OK in the "Measurement Process" display. The data overview is then displayed. 6.1.2.
Advanced Operations Refer to Table 11 for detailed information on the Edit formula keys. Note: The evaluation formula is built up successively in the display in accordance with the input. Note: The arrow key deletes the most recently entered element of the formula.
Advanced Operations Table 11 Edit formula key descriptions (continued) Screen Key +– ÷x >=< Description Press +– ÷x, to enter a mathematical operation. Select the operation and press OK to confirm. The available choice of mathematical operations depends on the defined formula. This means that functions such as "( )" or "ln"/"log" etc.
Advanced Operations Select Conc. key If an already defined formula, in this case C1, is to be taken into account in the formula for C2, press Select conc. Enter the number of the formula (e.g. 1 for C1) and press OK to confirm. Cn can now be linked with a mathematical operation. Note: The Cn concentrations that are to be calculated are numbered in sequence: C1, C2, C3, etc. Note: When the first Cn formula has been defined, the Formula list is automatically extended by Cn+1. 6.1.2.4 Enter variables 1.
Advanced Operations 6.1.3 Select a user program 1. Press User Programs in the Main Menu to view an alphabetical list of user programs with program numbers. The User Programs list will appear. Note: Use the scroll bar to scroll through the list quickly. Note: Highlight the selection by pressing it or press Select by Number to search for the program by number. 2. Press Start to run the program. 6.1.
Advanced Operations 6.1.4.1 Add to Favorites 1. Press Add to Favorites and press OK to confirm. The program is added to the Favorites. 6.1.4.2 Edit 1. Press Edit and press OK to confirm. An overview of the specifications of the programmed test is displayed. More information about the input options is provided in the section 6.1.2 on page 72. 6.1.4.3 Delete 1. Press Delete and press OK to confirm. The program is deleted from the list of User Programs.
Advanced Operations 6.2 Favorite Programs The most frequently used tests/methods in the Stored Programs menu and the User Programs menu can also be added to the list of favorites to simplify their selection. To add Stored Programs and/or User Programs to the favorites list or the favorite programs, see section 6.1.4 on page 79. 6.2.1 Recall a favorite program 1. Press Favorite Programs in the Main Menu. The Favorite Programs list will appear. 2. Highlight the selection by pressing it.
Advanced Operations 6.3 Standard Addition – monitoring/checking results The accuracy of measured values (their correspondence with the actual concentration of the analyte in the sample) and their precision (correspondence of the measurement results obtained from several samples containing the same concentration of the test analyte) can be determined or improved using the standard addition method. This method (also referred to as spiking) serves to identify sample-specific interference factors, e.g.
Advanced Operations e. Does the test require the sample to be at a certain temperature? f. Was the pH of the sample in the correct range? g. Is the pipette volume correct? 2. Check the used reagents by repeating the standard addition procedure with freshly prepared reagents. Detection rate Conclusion 100% The originally used reagents were defective. Check the standard solution: Repeat the standard addition procedure with a freshly prepared standard solution.
Advanced Operations Peak volume/sample volume methods 1. Select Stored Programs in the main menu. Select the required program. 2. Press Start. 3. Analyze a sample without added standard solution in accordance with the instructions in the Procedures Manual. When the measurement is complete, leave the sample cuvette/cell in the cuvette/sample cell holder. 4. Press Options>More>Standard Addition. An overview of the data of the standard addition procedure is displayed. 5.
Advanced Operations 6. Press the key of the value to change it. Use the alphanumeric keypad to change the value. Press OK to confirm. 7. Press the keys to enter the standard addition volumes. Use the alphanumeric keypad to enter the new data and press OK. Description of the table of readings • The first column shows the standard addition volume. 0 mL stands for a sample containing no added standard. • The second column shows the reading of the samples with and without added standard.
Advanced Operations 11. After all the standard addition solutions have been measured, press Graph. The regression line through the standard addition data points is displayed. The correlation coefficient r2 indicates how close the data points are to the line. If the correlation coefficient = 1, the curve is linear. The concentration shown above the curve is the estimated concentration of the sample without the added standard. Note: In the curve menu, the name on the Curve key switches to Table.
Advanced Operations 6.4 Single Wavelength (absorbance, concentration and transmittance measurements) The Single Wavelength mode can be used in three ways. For sample measurements at a single wavelength, the instrument can be programmed to measure the absorbance, % transmittance or concentration of the analyte. Absorbance measures the amount of light absorbed by the sample, in units of absorbance.
Advanced Operations Table 12 Single wavelength setup options (continued) Options Description Instrument Setup Basic data of the instrument, see section 5.2 on page 27. Concentration factor: 1. Press Concentration Factor: Off in the Options menu. Press On to highlight this feature. 2. Press the ”Factor“ key and use the alphanumeric keypad to enter the factor by which absorbance readings are to be multiplied. Press the ”Unit“ key to select the units for concentration measurements or to create a new unit.
Advanced Operations 6.4.2 Take single wavelength measurements 1. Insert the blank cuvette/cell into the cuvette/sample cell holder. Press Zero. Note: The Read key is only active after the zero measurement has been completed. 2. Insert the sample cuvette/cell into the cuvette/sample cell holder. Press Read. 3. For data storage, see section 5.3.1 on page 37.
Advanced Operations 6.5 Multi-Wavelength mode – measurements at more than one wavelength In the multi-wavelength mode, absorbance values can be measured at up to four wavelengths and the results can be mathematically processed to obtain sums, differences and relationships. Absorbance measures the amount of light absorbed by the sample, in units of absorbance. % Transmittance measures the percent of the original light that passes through the sample and reaches the detector.
Advanced Operations Table 13 Multi-wavelength setup options (continued) Options Description Recall Data Call up saved measurement data or time courses, see section 5.3 on page 37. Instrument Setup Basic data of the instrument, see section 5.2 on page 27. λ / Absorbance formula: 1. Press Absorbance Formula. 2. The formula selected in the top key determines the number of wavelength and coefficent keys that will appear below.
Advanced Operations 3. To change a wavelength, press one of the ”λx:“ keys. Enter the desired wavelength coefficient into the numeric keypad. Press OK to confirm. 4. To change a coefficient, press one of the ”KX:“ keys. Enter the desired coefficient into the numeric keypad. Press OK to confirm. Note: The instrument allows entry of up to 5 significant digits, with a maximum of 4 significant digits after the decimal point. Concentration factor: 1. Press Concentration Factor: Off in the Options menu.
Advanced Operations 6.5.2 Complete a measurement in the multi wavelength mode 1. Insert the blank cuvette/cell into the cuvette/sample cell holder. Press Zero. Note: The Read key does not become active until the zero measurement has been completed. 2. Insert the sample cuvette/cell into the cuvette/sample cell holder. Press Read. 3. For data storage, see section 5.3.1 on page 37.
Advanced Operations 6.6 Time course of absorbance/transmittance The Time Course Mode is used to collect data in either absorbance or transmittance for a user-specified length of time. After the data are collected, they can be displayed in either graphic or tabular format. 6.6.1 Time course setup parameters Press Time Course mode in the Main Menu. Press Options to configure parameters.
Advanced Operations Time & interval: 1. Press Time & Interval in the Options menu. 2. Input the total time and the reading time and press OK to confirm. Note: In total 500 measuring steps are possible. To select a total time and a time interval that would cause this number of measurements to be exceeded, the time interval is defined automatically and the OK key is inactivated. Scale & units: 1. Press Scale & Units in the Options menu. 2. Highlight Abs or %T as the required units. 3.
Advanced Operations • Select Mark to mark the next data point collected. This mark is not used by the instrument, but is available for the user and may indicate a significant event, such as the addition of a sample or other reagent. The mark is also shown in the table. • Select Stop to stop taking sample readings. 6.6.
Advanced Operations 6.6.3.1 Navigation of a time scan or a time scan analysis After a time scan has been completed, the time and the absorbance/transmittance data are displayed as a curve. Where the cursor is positioned on the curve, the elapsed time up to this point and the corresponding absorbance are highlighted. Table 15 Navigating the time scan Cursor Function/ Zoom Function Description Delta mode: A second cursor is highlighted.
Advanced Operations 6.7.1 Instrument information 1. Press Instrument Information in the System Checks menu. 2. The model, serial number and software version are displayed. 6.7.2 Upgrade of the instrument software To obtain the software for the update from the Internet at www.hach.com: 1. Go to http://www.hach.com. 2. On the DR 2800 product page, click Lab System Software/Software Update Downloads under Downloads. 3.
Advanced Operations 6.7.3 Optical checks For each optical check, the measured results must be evaluated against user-specific requirements. The check options do not define tolerances. 1. Press Optical Checks in the System Checks menu. The Optical Checks menu contains programs for checking the wavelength accuracy, stray light and photometric accuracy.
Advanced Operations 6.7.3.2 Stray light check The Stray Light test is used to measure the stray light in the instrument at 340 nm. 1. Press Stray Light Check in the Optical Checks menu. 2. Remove any cuvette/sample cell from the cell compartment. 3. Insert the adapter (A) for 10-mm rectangular cells in the cell compartment #2. Press Zero. 4. Insert the sample cuvette/cell or reference filter into the cell compartment #2. Close the cell compartment. Press Start. 5.
Advanced Operations 6.7.3.3 Absorbance check The Absorbance Check test is used to test the photometric accuracy and repeatability of the instrument. This test can be used to test absorbance at any wavelength by a specific sample or test filter set (Refer to section 6.7.3.4 on page 102). 1. Press Absorbance Check in the Optical Checks menu. 2. Press λ to input the wavelength. 3. Input the wavelength and press OK to confirm. 4. Remove any cuvette/sample cell from the cell compartment and press Zero. 5.
Advanced Operations 6.7.3.4 Verification kit The Verification Kit, LZV537, (see Section 9 on page 119) is designed for periodic monitoring of scattered light, photometric accuracy and the wavelength accuracy of the spectrophotometers. When results exceed allowable tolerances (given in the quality control certificate), contact the manufacturer. 1. Press Verification Kit in the Optical Checks menu. 2. Press Nominal Values. 3. Press Edit.
Advanced Operations 7. Remove any cuvettes/cells from the cell compartment and press Start. 8. Insert the different filter in the given order one after the other. Press Next after inserting a filter. After the last measurement the results are displayed. 9. Press PC & Printer icon to send the data to a USB memory stick, PC or to a printer. The files will be stored automatically as CSV file (Comma Separated Value). The file name will be formatted as “Verification.csv“.
Advanced Operations 6.7.4 Output checks If a printer is connected a test printing of the current screen will be printed. 6.7.5 Lamp history The Lamp History menu provides the amount of time that the lamp has been on (Hours). After a lamp is replaced and the Lamp History is reset, the display of the total operating time is reset to 0. 1. Press Lamp History in the System Checks menu. 2. Press Reset VIS and the Visible Lamp will be reset. 3. Press OK to return to System Checks. 6.7.
Advanced Operations 6.7.7 Service time In order to ensure a regular inspection, an automatic memory reference for the service times can be entered. After switching the instrument on this memory reference will be activated and indicated at the appropriate time. 1. Press Service Time in the System Checks menu. 2. Select On and then Last Service to enter the date of the last inspection. 3. Press OK to confirm. 4. Select Next Service to determine a specific period of time up to the next inspection. 5.
Advanced Operations 6.7.8 Instrument Backup Before the next service date the Instrument Backup menu offers the possibility to store all programs, measuring data, Operator ID, Sample ID, passwords and all adjustable data on a USB stick. 1. Press Instrument Backup in the System Checks menu. 2. Connect the USB memory stick (section 3.4 on page 13). 3. Press Store to start a Backup. Note: If the USB stick is not connected, the message "Please insert USB Memory" is displayed.
Advanced Operations If the file was stored the message Instrument Backup is stored to USB stick will be displayed. 4. Press OK to return to the System Checks menu. Restore backup data: Important Note: All current data will be overwritten when restoring the Backup file! 1. Press Instrument Backup in the System Checks menu. 2. Connect the USB memory stick containing the Backup (section 3.4 on page 13). 3. Press Restore to pass back the data.
Advanced Operations 4. Press OK to confirm after the message “Instrument Backup from S/N XXXXXXX. Restore?“ is displayed. 5. After the backup start the instrument again.
Section 7 Maintenance CAUTION Potential Chemical, Biological Eye and Skin Hazards. Only qualified personnel should conduct the tasks described in this section of the manual. Important Note: Remove any cuvettes/cells that are still in the instrument and dispose of them or their contents using an approved disposal method. 7.1 Cleaning requirements CAUTION Potential Pinch, Eye, Burn and Chemical Hazards. Always disconnect power from the instrument before attempting any cleaning operations.
Maintenance 7.2 Insert or change of the battery 1. Remove the cuvette from the cell compartment. 2. Switch the instrument off. 3. Unplug the power cord. 4. Turn the instrument over carefully and place it on a soft surface. 5. Use a screwdriver or a coin to remove the screw from the cover. 6. Remove the cover and place it to one side. CAUTION For quality and safety reasons, only the manufacturer’s lithium batteries (LZV551) should be used for this instrument.
Maintenance 8. Push in the battery so that the contacts of the instrument can be plugged into the socket of the battery. Important Note: Take care that the battery contacts are always clean. Soiled contacts can result in contact heating and voltage drop, interfering with the proper operation of the spectrophotometer. 9. Use a screwdriver or a coin to replace the screw securing the cover. 10. Carefully stand the instrument upright. 11. Plug in power supply again – the Instrument is now ready for use.
Maintenance • The performance of the battery decreases with the temperature. 7.2.1.2 Optimal operation of the battery The battery is charged automatically when the plug-in power supply on the back of the instrument is connected and is plugged into a mains socket (100–240 Volt/50–60 Hz). CAUTION Use only the manufacturer's specified external power supply to operate the instrument and charge the internal lithium battery pack.
Maintenance 7.3 Lamp replacement CAUTION To avoid a possible electric shock, disconnect the instrument from the power source before servicing the lamp. 1. Remove the cuvette from the cell compartment. 2. Switch the instrument off. 3. Unplug the power cord. WARNING Burn Hazard. Wait until the lamp cools down. Contact with the hot lamp can cause burns. 4. Turn the instrument over carefully and place it on a soft surface. 5. Use a screwdriver or a coin to remove the screw from the cover. 6.
Maintenance WARNING Burn Hazard. Wait until the lamp cools down. Contact with the hot lamp can cause burns. 9. Lift the lamp fitting carefully. 10. Put both screws to one side. 11. Push up on the pressure spring. 12. Remove the halogen lamp complete with the plug panel.
Maintenance 13. Carefully unplug the halogen lamp from the plug panel. Important Note: Hold the lamp by the fitting only. Avoid touching the glass, as substances on the skin can bake onto the lamp bulb and thus accelerate the aging process of the lamp. 14. Plug a new halogen lamp to the panel. 15. Insert the halogen lamp with the half rounded part pointing down. 16. Press the plug with slight pressure into the direction of the halogen lamp and push the pressure spring down, so that it will engage. 17.
Maintenance 116
Section 8 Troubleshooting Problem/Display screen Likely Cause Action Attention! Please insert the light shield. Measurements with barcode cuvettes generally require the light shield. Insert the light shield. Press OK. Absorbance > 3.5! The measured absorbance exceeds 3.
Troubleshooting 118
Section 9 Replacement Parts Description Cat. No.
Replacement Parts 120
Section 10 Contact Information HACH Company World Headquarters P.O. Box 389 Loveland, Colorado 80539-0389 U.S.A. Tel (800) 227-HACH (800) -227-4224 (U.S.A. only) Fax (970) 669-2932 orders@hach.com www.hach.com Repair Service in the United States: HACH Company Ames Service 100 Dayton Avenue Ames, Iowa 50010 Tel (800) 227-4224 (U.S.A. only) Fax (515) 232-3835 Repair Service in Canada: Hach Sales & Service Canada Ltd.
Contact Information 122
Section 11 Limited Warranty Hach Company warrants its products to the original purchaser against any defects that are due to faulty material or workmanship for a period of one year from date of shipment unless otherwise noted in the product manual. In the event that a defect is discovered during the warranty period, Hach Company agrees that, at its option, it will repair or replace the defective product or refund the purchase price excluding original shipping and handling charges.
Limited Warranty 124
Index A Absorbance Check ................................................ 101 Absorbance Formula ......................................... 90, 91 Alphanumeric keypad .............................................. 25 Audio signals ........................................................... 29 Marking .................................................................... 96 Measurement Process .................................55, 56, 72 Measuring sequence ................................................
V Variables ......................................................55, 57, 78 Verification Kit ........................................................ 102 Version ............................................................... 55, 56 View Table ............................................................... 94 VIS-Lamp Lamp Control Lamp History ................................................... 104 W Warranty ................................................................ 123 Wavelength ................
