Instruction Manual TECHNICAL SERVICE CONTACTS Australia: Tel. (03) 9769.0666 • Fax (03) 9769.0699 e-mail: hannains@hannainst.com.au C 214 China: Tel. (10) 88570068 • Fax (10) 88570060 e-mail: hannachina@vip.sina.com Multiparameter Bench Photometer Egypt: Tel. & Fax (02) 2758.683 e-mail: hannaegypt@go.com.eg Germany: for Tel. (07851) 9129-0 • Fax (07851) 9129-99 e-mail: hannager@aol.com Wastewater Treatment Greece: Tel. (210) 823.5192 • Fax (210) 884.0210 e-mail: hannagr@otenet.
Dear Customer, Thank you for choosing a Hanna product. Please read this instruction manual carefully before using the meter. This manual will provide you with the necessary information for the correct use of the instrument. If you need additional technical information, do not hesitate to e-mail us at tech@hannainst.com. These instruments are in compliance with directives. TABLE OF CONTENTS NITROGEN, TOTAL LR .................... 37 NITROGEN, TOTAL HR ................... 42 OXYGEN DEMAND, CHEMICAL LR ....
CE DECLARATION OF CONFORMITY PRELIMINARY EXAMINATION Please examine this product carefully. Make sure that the instrument is not damaged. If any damage occured during shipment, please notify your Dealer. Each Meter is supplied complete with: • Five Sample Vials • Two 9 V Batteries • 12 VDC Transformer (HI 710005 or HI 710006) • Instruction Manual Note: Save all packing material until you are sure that the instrument works correctly.
GENERAL DESCRIPTION ® C 99 & C 200 Series is a line of 17 different bench, microprocessorbased photometers to measure more than 50 different parameters in water and wastewater.
STANDARD METHODS Description Range Method Ammonia LR Ammonia HR Chlorine, Free Chlorine, Total Nitrate Nitrogen, Total Nitrogen, Total HR COD LR COD MR COD HR Phosphorus, Reactive Phosphorus, Acid Hydrolyzable Phosphorus, Total Phosphorus, Reactive HR Phosphorus, Total HR 0.00 to 3.00 mg / L 0 to 100 mg / L 0.00 to 5.00 mg / L 0.00 to 5.00 mg / L 0.0 to 30.0 mg / L 0.0 to 25.0 mg / L 10 to 150 mg / L 0 to 150 mg / L 0 to 1500 mg / L 0 to 15000 mg / L 0.00 to 5.00 mg / L 0.00 to 5.00 mg / L 0.00 to 3.
PRINCIPLE OF OPERATION I I Absorption of Light is a typical phenomenon of interaction between electromagnetic radiation and matter. When a light beam crosses a substance, some of the radiation may be absorbed by atoms, molecules or crystal lattices.
INTERFACE WITH PC With terminal programs such as, for example, Telix®, Windows Terminal®, it is possible to remotely control your Hanna Instruments bench meter. Use HI 920010 cable to connect the meter to the PC, start the terminal program and set the communication options as follows: 8, N, 1, no flow control.
FUNCTIONAL DESCRIPTION FRONT PANEL • Wait for a few seconds and the display will show “-0.0-”. Now the meter is zeroed and ready for measurement. • Remove the blank vial. • Place the sample vial into the holder and push it completely down. • Press READ DIRECT and "SIP" will blink on the display.
• At the end of the digestion place the vials carefully in the test tube rack and allow to cool to room temperature. Warning: as the vials are still hot, be careful in handling them. • Select the program number corresponding to Total Phosphorus High Range on the secondary LCD by pressing PROGRAM t and s. • Remove the cap from the vials and add exactly 2.0 mL of Sodium Hydroxide (NaOH) Solution 1,54 N to each vial, while keeping the vials at a 45-degree angle.
The lamp is not working properly. Contact your dealer or the nearest Hanna Customer Service Center. The lamp is not working properly. Contact your dealer or the nearest Hanna Customer Service Center. This indicates that the meter has lost its configuration. Contact your dealer or the nearest Hanna Customer Service Center. Reagent Blank Correction: This method needs a reagent blank correction. A single blank vial may be used more than once; the blank vial is stable up to one day (room temperature).
PHOSPHORUS, TOTAL HIGH RANGE SPECIFICATIONS Range Resolution Precision Typical EMC Deviation Light Source Method 0.0 to 100.0 mg/L (as PO43-) 0.1 mg/L ±3.0 @ 75.0 mg/L ±0.1 mg/L Tungsten lamp with narrow band interference filter @ 420 nm Adaptation of the Standard Methods for the Examination of Water and Wastewater, 20th edition, 4500-P C, vanadomolybdophosphoric acid method. A persulfate digestion converts organic and condensed inorganic forms of phosphates to orthophosphate.
TIPS FOR AN ACCURATE MEASUREMENT The instructions listed below should be carefully followed during testing to ensure most accurate results. • Place the sample vial into the holder and push it completely down. • Press READ DIRECT and "SIP" will blink on the display.
• Select the program number corresponding to Reactive Phosphorus High Range on the secondary LCD by pressing PROGRAM t and s. • Remove the cap from two Reagent Vials. • Add exactly 5.0 mL of sample to one vial (sample vial), and 5.0 mL of deionized water to the other vial (blank vial), while keeping the vials at a 45-degree angle. 2. In order to measure exactly e.g. 0.5 mL of reagent with the 1 mL syringe: (a) push the plunger completely into the syringe and insert the tip into the solution.
• Proper use of the powder reagent packet: (a) use scissors to open the powder packet; (b) push the edges of the packet to form a spout; (c) pour out the content of the packet. PHOSPHORUS, REACTIVE HIGH RANGE SPECIFICATIONS Range Resolution Precision Typical EMC Deviation Light Source c b a ) ( ) ( ) ( Method USING VIALS • Never insert hot vials in the instrument, or the cuvet holder may become damaged.
• Press TIMER and the display will show the countdown prior to the measurement. Alternatively, wait for 3 minutes and press READ DIRECT. In both cases "SIP" will blink during measurement. • The instrument directly displays concentration in mg/L of phosphate (PO43-) on the Liquid Crystal Display. The method detects free (orthophosphate), condensed inorganic forms (meta-, pyro- and other polyphosphates) and organic forms of phosphates present in the sample.
• Do not let the reacted sample stand too long after reagent is added, or accuracy will be lost. • It is possible to take multiple readings in a row, but it is recommended to take a new zero reading for each sample and to use the same vial for zeroing and measurement where possible (for most precise results follow carefully the measurement procedures). • All the reaction times reported in this manual are referred to 20°C (68°F).
• Preheat the Hanna Reactor C 9800 to 150 °C (302°F). For correct use of the reactor follow Reactor Instruction Manual. Use of the optional HI 740217 safety shield is strongly recommended. Do not use an oven or microwave because leaking samples can generate a corrosive and possibly explosive atmosphere. • Remove the cap from a Reagent Vial. • Add exactly 5.0 mL of sample to the vial, while keeping the vial at a 45-degree angle. • Add the content of one packet of Potassium Persulfate for Phosphorus analysis.
• The letter refers to: A : ammonia N : nitrate; total nitrogen P : phosphorus (no letter) : chemical oxygen demand (COD) and generic empty vials. • The cap color allows distinguishing different vials that are used to measure the same analyte (indicated by the letter code), but for a different range, or between different vials used in a single measurement procedure. Note that different parameters use sometimes the same vials (thus with the same letter and same cap color).
• The instrument directly displays concentration in mg/L of phosphate (PO43-) on the Liquid Crystal Display. The method detects free (orthophosphate) and condensed inorganic forms (meta-, pyro- and other polyphosphates) of phosphates present in the sample. • To convert the reading to mg/L of P2O5 , multiply by a factor of 0.748. • To convert the reading to mg/L of phosphorus (P) concentration, multiply by a factor of 0.326.
OPERATIONAL GUIDE POWER CONNECTION Plug the 12VDC adapter (HI 710005 - 110VDC, or HI 710006 220VDC) into the DC socket. Plug the adapter into the outlet. Alternatively, remove the battery cover on the back of the meter; attach 2 fresh 9V batteries and replace the cover. Note: Insure the main line is surge protected. Note: Always turn the meter off before unplugging it to insure no data is lost. • Mark the vial with a pencil on the white band to place an orientation sign.
• Preheat the Hanna Reactor C 9800 to 150 °C (302°F). For correct use of the reactor follow Reactor Instruction Manual. Use of the optional HI 740217 safety shield is strongly recommended. Do not use an oven or microwave because leaking samples can generate a corrosive and possibly explosive atmosphere. • After the desired program number appears on the secondary display, follow the measurement procedure described in the related chapter.
AMMONIA LOW RANGE PHOSPHORUS, ACID HYDROLYZABLE SPECIFICATIONS SPECIFICATIONS Range Resolution Precision Typical EMC Deviation Light Source Range Resolution Precision Typical EMC Deviation Light Source Method 0.00 to 3.00 mg/L (as NH3-N) 0.01 mg/L ±0.03 @ 1.50 mg/L ±0.01 mg/L Tungsten lamp with narrow band interference filter @ 420 nm Adaptation of the ASTM Manual of Water and Environmental Technology, D1426-92, Nessler method.
• The instrument directly displays concentration in mg/L of phosphate (PO43-) on the Liquid Crystal Display. • To convert the reading to mg/L of P2O5, multiply by a factor of 0.748. • To convert the reading to mg/L of phosphorus (P) concentration, multiply by a factor of 0.326. Note: for accurate measurements 1) wash glassware only with phosphate-free detergents 2) clean all glassware with 1 : 1 hydrochloric acid solution and rinse with deionized water.
• The instrument directly displays concentration in mg/L of ammonia nitrogen (NH3-N) on the Liquid Crystal Display. • To convert the reading to mg/L of ammonia (NH3), multiply by a factor of 1.214. INTERFERENCES Interference may be caused by: • Organic compounds like: chloramines, various aliphatic and aromatic amines, glycine or urea above 10 ppm N (positive error). To eliminate these interferences distillation is required. • Organic compounds like: aldehydes, alcohols (e.g. ethanol) or acetone above 0.
PHOSPHORUS, REACTIVE AMMONIA HIGH RANGE SPECIFICATIONS SPECIFICATIONS Range Resolution Precision Typical EMC Deviation Light Source Range Resolution Precision Typical EMC Deviation Light Source Method 0.00 to 5.00 mg/L (as PO43-) 0.01 mg/L ±0.05 @ 2.50 mg/L ±0.01 mg/L Tungsten lamp with narrow band interference filter @ 610 nm Adaptation of the EPA method 365.2 and Standard Methods for the Examination of Water and Wastewater, 20th edition, 4500-P E, ascorbic acid method.
• Mark the vial with a pencil on the white band to place an orientation sign. Use this sign to insert the test vial always in the same position into the holder. • Place the vial into the holder and push it completely down. • Select the program number corresponding to Oxygen Demand, Chemical HR (COD) on the secondary LCD by pressing PROGRAM t and s. • Place the blank vial into the holder and push it completely down. • Press ZERO and "SIP" will blink on the display.
• Choose a homogeneous sample. Samples containing settleable solids need to be homogenized with a blender. • Preheat the Hanna Reactor C 9800 to 150 °C (302°F). For correct use of the reactor follow Reactor Instruction Manual. Use of the optional HI 740217 safety shield is strongly recommended. Do not use an oven or microwave because leaking samples can generate a corrosive and possibly explosive atmosphere. • Remove the cap from two Reagent Vials. • Add exactly 0.
CHLORINE, FREE OXYGEN DEMAND, CHEMICAL HIGH RANGE SPECIFICATIONS SPECIFICATIONS Range Resolution Range Resolution Precision Typical EMC Deviation Light Source Precision Typical EMC Deviation Light Source Method 0.00 to 5.00 mg/L 0.01 mg/L from 0.00 to 0.99 mg/L; 0.1 mg/L above 0.99 mg/L ±0.04 @ 1.00 mg/L ±0.01 mg/L Tungsten lamp with narrow band interference filter @ 525 nm Adaptation of the EPA method 330.
• Select the program number corresponding to Oxygen Demand, Chemical MR (COD) on the secondary LCD by pressing PROGRAM t and s. • Place the vial into the holder and push it completely down. • Place the blank vial into the holder and push it completely down. • Press ZERO and “SIP” will blink on the display. • Press ZERO and "SIP" will blink on the display. • Wait for a few seconds and the display will show “-0.0-”. Now the meter is zeroed and ready for measurement. • Remove the blank vial.
Oxidized forms of Chromium and Manganese Ozone (O3) • Choose a homogeneous sample. Samples containing settleable solids need to be homogenized with a blender. Alkalinity above 250 mg/L CaCO3 or acidity above 150 mg/L CaCO3 will not reliably develop the full amount of color or it may rapidly fade. To resolve this, neutralize the sample with diluted HCl or NaOH. In case of water with hardness greater than 500 mg/L CaCO3, shake the sample for approximately 2 minutes after adding the powder reagent.
OXYGEN DEMAND, CHEMICAL MEDIUM RANGE CHLORINE, TOTAL SPECIFICATIONS SPECIFICATIONS Range Resolution Precision Typical EMC Deviation Light Source Range Resolution Method 0 to 1500 mg/L 1 mg/L ±22 @ 1000 mg/L ±1 mg/L Tungsten lamp with narrow band interference filter @ 610 nm Adaptation of the USEPA 410.4 approved method for the COD determination on surface waters and wastewaters. Oxidizable organic compounds reduce the dichromate ion (orange) to the chromic ion (green).
• Place the vial into the holder and push it completely down. • Select the program number corresponding to Oxygen Demand, Chemical LR (COD) on the secondary LCD by pressing PROGRAM t and s. • Press ZERO and “SIP” will blink on the display. • Place the blank vial into the holder and push it completely down. • Press ZERO and "SIP" will blink on the display. • Wait for a few seconds and the display will show “-0.0-”. Now the meter is zeroed and ready for measurement. • Remove the vial.
• Choose a homogeneous sample. Samples containing settleable solids need to be homogenized with a blender. • Preheat the Hanna Reactor C 9800 to 150 °C (302°F). For correct use of the reactor follow Reactor Instruction Manual. Use of the optional HI 740217 safety shield is strongly recommended. Do not use an oven or microwave because leaking samples can generate a corrosive and possibly explosive atmosphere. • Remove the cap from two Reagent Vials. • Add exactly 2.
NITRATE OXYGEN DEMAND, CHEMICAL LOW RANGE SPECIFICATIONS SPECIFICATIONS Range Resolution Precision Typical EMC Deviation Light Source Range Resolution Precision Typical EMC Deviation Light Source Method 0.0 to 30.0 mg/L (as NO3--N) 0.1 mg/L ±0.5@ 25.0 mg/L ±0.1 mg/L Tungsten lamp with narrow band interference filter @ 420 nm Chromotropic acid method. The reaction between nitrate and the reagents causes a yellow tint in the sample. Method REQUIRED REAGENTS Code Description Q.ty/test Q.
• Place the blank vial into the holder and push it completely down. • Press TIMER and the display will show the countdown prior to the measurement. Alternatively, wait for 5 minutes and press ZERO. In both cases "SIP" will blink during measurement. • Wait for a few seconds and the display will show “-0.0-”. Now the meter is zeroed and ready for measurement. • Replace the cap tightly and invert the vial 10 times. This is the blank. Warning: as the vial becomes hot during mixing, be careful in handling it.
• Place the vial into the holder and push it completely down. Ensure that the vial orientation respect to the holder is the same as the blank. • Remove the cap from the vials and add the content of one packet of HI 93767-0 Total Nitrogen Reagent to each vial. Replace the cap tightly and shake gently the vials for 15 seconds. • Press TIMER and the display will show the countdown prior to the measurement. Alternatively, wait for 5 minutes and press READ DIRECT.
• Replace the cap tightly and shake vigorously the vials for about 30 seconds until all the powder is completely dissolved. NITROGEN, TOTAL LOW RANGE SPECIFICATIONS Range Resolution Precision Typical EMC Deviation Light Source • Insert the vials into the reactor and heat them for 30 minutes at 105°C. Note: to obtain most accurate results, it is strongly recommended to remove the vials frome the reactor after exactly 30 minutes.
MEASUREMENT PROCEDURE MEASUREMENT PROCEDURE Before starting to use the reagent kit it is recommended to read carefully all the instructions and the Health & Safety Data Sheet (HSDS). Pay particular attention to all warnings, cautions and notes. Failure to do so may result in serious injury to the operator. Reagent Blank Correction: This method needs a reagent blank correction.
NITROGEN, TOTAL HIGH RANGE • Replace the cap tightly and shake the vials vigorously for about 30 seconds until all the powder is completely dissolved. SPECIFICATIONS Range Resolution Precision Typical EMC Deviation Light Source Method 10 to 150 mg/L 1 mg/L ±3 @ 75 mg/L ±1 mg/L Tungsten lamp with narrow band interference filter @ 420 nm Chromotropic acid method. A persulfate digestion converts all forms of nitrogen to nitrate.
• Remove the cap from the vials and add the content of one packet of HI 93767-0 Total Nitrogen Reagent to each vial. Replace the cap tightly and shake gently the vials for 15 seconds. • Place the blank vial into the holder and push it completely down. • Press TIMER and the display will show the countdown prior to the measurement. Alternatively, wait for 5 minutes and press ZERO. In both cases "SIP" will blink during measurement.
