Instruction Manual HI 221 HI 223 pH/mV/ºC Bench Meters with Calibration Check POOR GOO D SLO W FAST pH ATC C CAL C LOG Func tions CF GLPM C RANG E CFM/G LP RCL Selec t pH/O RP Store Log Inf ormati Reca on ll Set Ins Log Inform Clear ation me Lotru g/Cal nt Param Show Inform ete GLP ation rs Inform ation LOG pH Ca libratio n RCL CAL SET/C LR Start Calib ration Selec st t 1 Bu ffer Confi rm 1 st Buffe Selec r t 2 ndBu ffer Confi rm 2 nd Buffe r / CFM / CFM HI 22 Micro 3 Cal proc ibra
Dear Customer, Thank you for choosing a Hanna Instruments product. Please read this instruction manual carefully before using the instrument. This manual will provide you with the necessary information for correct use of the instrument, as well as a precise idea of its versatility. If you need additional technical information, do not hesitate to e-mail us at tech@hannainst.com or turn to the back cover for our worldwide contact list. These instruments are in compliance with directives.
PRELIMINARY EXAMINATION Remove the instrument from the packing material and examine it carefully to make sure that no damage has occurred during shipping. If there is any damage, notify your Dealer or the nearest Hanna Customer Service Center. Each instrument is supplied complete with: HI 1131P Glass-body Combination pH Electrode with 1 m (3.3 ) cable HI 7669/2W Temperature Probe HI 76404 Electrode Holder pH 4.01 & 7.
FUNCTIONAL DESCRIPTION Front FrontPanel Panel Electrode Response Electrode Condition Primary LCD Secondary LCD ATC pH 1 POOR GOOD SLOW C FAST 10 2 Functions 3 5 7 RANGE CFM GLP 4 CAL 6 C C LOG RCL pH Calibration Select pH/ORP LOG Store Log Information RCL Recall Log Information SET/CLR Set Instrument Parameters Clear Log/Cal Information CFM/GLP Show GLP Information CAL Start Calibration / Select 1 Buffer CFM / CFM st 8 RANGE st Confirm 1 Buffer Select 2 nd Buffer nd
HI 221 SPECIFICATIONS 2.00 to 16.00 pH ± 699.9 mV ± 2000 mV Range 20.0 to 120.0 °C 0.01 pH 0.1 mV (± 699.9 mV) 1 mV (± 2000 mV) Resolution 0.1 °C ± 0.01 pH Accuracy @ 20 °C/68 °F ± 0.2 mV (± 699.9 mV) ± 1 mV (± 2000 mV) ± 0.4 °C Calibration Check Yes Computer Interface Opto-isolated RS232 pH Calibration 1 or 2 point, 7 buffers available (1.68, 4.01, 6.86, 7.01, 9.18, 10.01, 12.
HI 223 SPECIFICATIONS 2.00 to 16.00 pH 2.000 to 16.000 pH ± 999.9 mV ± 2000 mV Range 20.0 to 120.0 °C 0.01 pH 0.001 pH 0.1 mV (± 999.9 mV) 1 mV (± 2000 mV) Resolution 0.1 °C ± 0.01 pH ± 0.002 pH ± 0.2 mV (± 699.9 mV) ± 0.5 mV (± 999.9 mV) ± 1 mV (± 2000 mV) Accuracy @ 20 °C/68 °F ± 0.4 °C Calibration Check Yes Computer Interface Opto-Isolated RS232 pH Calibration 1 or 2 point, 7 buffers available (1.68, 4.01, 6.86, 7.01, 9.18, 10.01, 12.
OPERATIONAL GUIDE POWER CONNECTION Plug the 12 VDC adapter into the power supply socket. Note: These instruments use non volatile memory to retain the pH, mV, temperature calibrations and all other settings, even when unplugged. Note: Make sure a fuse protects the mains line. ELECTRODE AND PROBE CONNECTIONS For HANNA P Type pH or ORP electrodes (with internal reference) connect the electrodes BNC to the socket on the back of the instrument and the pin to the appropriate socket.
The pH is displayed on the primary LCD and the temperature on the secondary LCD. pH POOR GOOD SLOW ATC C FAST The pH reading is out of range, the closest full-scale value will be displayed blinking on the primary LCD. It is also possible to view the mV reading by pressing the RANGE key.
The instrument automatically defaults to the ORP measurement mode if HANNA P type ORP electrode is detected. Submerge the ORP electrode tip (4 cm/1½) into the sample. Allow a few minutes for the reading to stabilize. The instrument displays the mV reading on the primary LCD. If the reading is out of range, the closest full-scale value will be displayed blinking on the primary LCD. TAKING TEMPERATURE MEASUREMENTS Connect the HI 7669/2W temperature probe and turn the instrument on.
PROCEDURE The user has a choice of 7 memorized buffers: pH 1.68, 4.01, 6.86, 7.01, 9.18, 10.01 and 12.45. It is always recommended to perform a two-point calibration, however the instrument also allows a one-point calibration, as described on page 11. TWO-POINT CALIBRATION For most applications it is recommended that pH 7.01 or 6.86 buffers be used as the first calibration point and pH 4.01 (for acidic samples) or pH 9.18/ 10.01 (for alkaline samples) as the second calibration point. Note: The pH 12.
0 Press the ARROW keys to select a different buffer value, if necessary. When the reading is stable and close to the selected buffer, the CFM tag will blink and if enabled, an audible signal will sound. ATC Press the CFM key to confirm the calibration. The instrument will ask for a second calibration buffer and display the measured pH on the first LCD line and the second calibration buffer on the second LCD line. ATC If necessary, press the ARROW keys to select a different buffer value.
ENHANCED CALIBRATION MESSAGES The stored calibration history to used issue error and warning messages during calibration to help ensure the highest accuracy. As electrode aging is normally a slow process, substantial changes from previous calibrations are likely due to a temporary problem with the electrode or buffers. Calibrating under these conditions will give measurement errors. ERROR MESSAGES Error messages appear if one or all of the calibration parameters are out of accepted windows.
2 ELECTRODE This message appears if the cleaning procedure performed as a result of the above two messages is found by the instrument to be unsuccessful. Replace the electrode. ATC WRONG BUFFER TEMPERATURE This message appears if the temperature of the buffer is outside the defined buffer temperature range.
a result of unacceptable offset variation or in the second calibration buffer as a result of unacceptable slope variation. This variation may result from dirt on the electrode or contaminated buffer. Refer to the electrode cleaning procedure or use fresh buffer. CAL CHECK CFM pH ATC BUFFER 1 BUFFER CLEAN ELECTRODE CONTAMINATED BUFFER This warning message appears in order to alert that the buffer could be contaminated. Refresh your buffer and continue the calibration procedure.
4 Also, when the instrument cannot evaluate the electrode response or pH 1.68 or pH 12.45 buffer were used as calibration buffer, the response gauge will be empty. If the electrode is in a very poor condition the first condition segment will blink. If electrode response is very slow the first response segment will blink. GOOD LABORATORY PRACTICE (GLP) GLP is a set of functions that allows the storage and retrieval of data regarding the maintenance and status of the electrode.
_ The date (mm.dd). _ The year (yyyy). _ The pH calibration offset. _ The pH calibration slope in mV/pH normalized to 25 °C. Note: If you calibrate using electrodes with pin the electrode condition and response gauges appear while the offset and slope are displayed. _ The first pH calibration buffer along with any warning messages issued while calibrating at this point. _ The second pH calibration buffer along with any warning messages issued while calibrating at this point.
6 _ The selected resolution of the instrument during calibration (HI 223 only). CAL pH RCL _ The calibration Alarm Time-Out status: if disabled or the number of days until the calibration alarm will be displayed or if expired (7 days ago) Press the GLP key at any time and the instrument will return to measuring mode. If calibration has not been performed, the instrument displays no CAL blinking.
LOGGING Up to 100 (HI 221) or 500 (HI 223) LOG samples can be stored in memory. LOGGING THE CURRENT DATA To store the current reading into memory press the LOG key while in measuring mode. The instrument will display the current date (mm.dd) on the primary LCD, the record number on the secondary LCD and the LOG tag will blink for a few seconds (see example below: record No.
8 Otherwise the instrument will display log data on the primary LCD and the last stored record number on the secondary LCD, along with the LOG and the RCL tags. Note: The LOG and RCL tags remain on LCD while instrument is in the viewing logged data mode. Press the RCL key at any time to return to measuring mode.
The date on the primary LCD and the dAtE message on the secondary LCD. The year on the primary LCD and the YEAr message on the secondary LCD. The calibration offset on the primary LCD and the oFFS message on the secondary LCD. The calibration slope on the primary LCD and the SLoP message on the secondary LCD. Note: Before displaying the Hour, Year, oFFS or SLoP messages, the record number is displayed for about one second.
0 The dEL message on the primary LCD and the selected record or date on the secondary LCD. The CFM and the DEL tags will blink: Press SET key to select the current record, the records from the selected date or all records to be deleted. the date. or ALL records. Press the ARROW keys to change the record or the date.
SETUP Setup mode allows viewing and modification of the following parameters: Calibration Alarm Time Out Instrument ID Current Time (hour & minute) Current Date (month & day) Current Year pH Resolution (HI 223 only) Beep Status Baud Rate (serial communication) Command Prefix (serial communication) To enter the Setup mode press the SET/CLR key while the instrument is in measuring mode. Press SET/CLR key to exit SETUP mode. Select a parameter with the ARROW keys.
2 Press the ARROW keys to change the instrument ID value. Press the CFM key to save the modified instrument ID value or press the CAL key to cancel without saving the instrument ID. Note: The instrument ID is downloaded to a PC as part of a logged data set to identify its origin. CURRENT TIME SET Press the CAL key when the current time is displayed. The hour will begin blinking. Press the ARROW keys to change the hour. Press the RANGE key. The minutes will begin blinking.
CURRENT YEAR SET Press the CAL key when the current year is displayed. The year will begin blinking. Press the ARROW keys to change the year. Press the CFM key to save the modified value or press the CAL key to cancel without saving the year. pH RESOLUTION SET (HI 223 ONLY) Press the CAL key when the pH resolution is displayed. The pH resolution (0.001 or 0.01) will begin blinking. Press the ARROW keys to change the pH resolution.
4 SERIAL COMMUNICATION BAUD RATE SET Press the CAL key when the baud rate is displayed. The baud rate (600, 1200, 2400, 4800 or 9600) will begin to blink. Press the ARROW keys to change the baud rate. Press the CFM key to save the modified baud rate or press the CAL key to cancel without saving the baud rate. SERIAL COMMUNICATION COMMAND PREFIX SET Press the CAL key when the command prefix is displayed. The command prefix (0 to 47) will begin to blink. Press the ARROW keys to change the command prefix.
containing ice and water. Wait a few minutes for the probe to stabilize. Press the CAL and LOG keys simultaneously and then switch the instrument on. After a few seconds, the instrument enters the temperature calibration mode. The CAL tag will come on. The primary LCD will display the measured temperature or the - - - - message if the measured temperature is out of range. The secondary LCD will display 0.0 °C (First calibration point). The tag will blink until the reading has stabilized.
6 mV CALIBRATION (FOR TECHNICAL PERSONNEL ONLY) All instruments are factory calibrated for mV. Hannas pH and ORP electrodes are interchangeable and mV calibration is not needed when replaced. If the mV measurements are not accurate, mV recalibration should be carried out. For an accurate recalibration, contact your dealer or the nearest Hanna Customer Service Center or follow the instructions below. A two or three point calibration can be performed at 0 mV, +600 mV and +1800 mV.
Note: Press the CAL key to leave the calibration mode at any time. If the measured value is not close to the calibration point, the WRONG tag will start to blink. In this case, check if the value set on the simulator is the same as the calibration value on the instrument. Set the proper value on the simulator. PC INTERFACE Data transmission from the instrument to the PC can be done with the HI 92000 Windows® compatible software (optional). The HI 92000 also offers graphing and on-line help.
8 SIMPLE RNG CAL CFM UPC DWC SET COMMANDS Is equivalent to pressing the RANGE key Is equivalent to pressing the CAL key Is equivalent to pressing the CFM/GLP key Is equivalent to pressing the UP arrow key Is equivalent to pressing the DOWN arrow key Is equivalent to pressing the SET/CLR key COMMANDS REQUIRING AN ANSWER pH? MV? TM? MDR PAR NSL GLP LOD xxx LOD ALL Causes the instrument to send the pH reading (Err 1 is sent if out of range). If the range is set to mV, Err 6 is received.
pH VALUES AT DIFFERENT TEMPERATURES Temperature has an effect on pH. The calibration buffer solutions are affected by temperature changes to a lesser degree than normal solutions. During calibration the instrument will automatically calibrate to the pH value corresponding to the measured or set temperature. TEMP pH V ALUES ºC ºF 1.68 4.01 6.86 7.01 9.18 10.01 12.45 0 32 1.67 4.01 6.98 7.13 9.46 10.32 13.42 5 41 1.67 4.00 6.95 7.10 9.39 10.24 13.21 10 50 1.67 4.00 6.92 7.
0 ELECTRODE CONDITIONING & MAINTENANCE PREPARATION Remove the protective cap. DO NOT BE ALARMED IF SALT DEPOSITS ARE PRESENT. This is normal with electrodes. They will disappear when rinsed with water. During transport, tiny bubbles of air may form inside the glass bulb affecting proper functioning of the electrode. These bubbles can be removed by shaking down the electrode as you would do with a glass thermometer.
For Refillable Electrodes: If the filling solution (electrolyte) is more than 2½ cm (1") below the fill hole, add HI 7082 or HI 8082 3.5M KCl Electrolyte Solution for double junction or HI 7071 or HI 8071 3.5M KCl+AgCl Electrolyte Solution for single junction electrodes using a plastic refilling pipette (HI 740157). As recommended by the LCD message on instrument on start-up, remove or loosen the fill hole screw on the electrode for better performance.
2 IMPORTANT: After performing any of the cleaning procedures, rinse the electrode thoroughly with distilled water, refill the reference chamber with fresh electrolyte (not necessary for gel-filled electrodes) and soak the electrode in HI 70300 or HI 80300 Storage Solution for at least 1 hour before taking measurements. TROUBLESHOOTING GUIDE SYMPTOMS Slow response/excessive drift. The reading drifts. Low electrode condition. Readings fluctuate up and down (noise).
SYMPTOMS AMPHE L E lectrodes do not respond to pH changes. The meter fails to calibrate or gives faulty readings. At startup the meter displays all LCD tags permanently. E xplicit warnings are displayed on the LCD during pH calibration. The electrode condition and response gauges are not displayed after calibration. The electrode condition and response gauges are empty. PROBLEM SOLUTION The battery is run down. Replace the electrode. Broken pH electrode. Replace the pH electrode.
4 Since the resistance of the pH electrode is in the range of 50-200 Mohm depending on the composition of the glass, the current across the membrane is in the pico Ampere range. Large currents can disturb the calibration of the electrode for many hours. For these reasons high humidity environments, short circuits and static discharges are detrimental to a stable pH reading. The pH electrodes life also depends on the temperature.
ACCESSORIES pH CALIBRATION SOLUTIONS HI 70004P HI 70007P HI 70010P HI 7001M HI 7004L HI 7006L HI 7007L HI 7009L HI 7010L HI 7001L HI 8004L HI 8006L HI 8007L HI 8009L HI 8010L pH 4.01 Buffer Sachets, 20 mL, 25 pcs pH 7.01 Buffer Sachets, 20 mL, 25 pcs pH 10.01 Buffer Sachets, 20 mL, 25 pcs pH 1.68 Buffer Solution, 230 mL pH 4.01 Buffer Solution, 500 mL pH 6.86 Buffer Solution, 500 mL pH 7.01 Buffer Solution, 500 mL pH 9.18 Buffer Solution, 500 mL pH 10.01 Buffer Solution, 500 mL pH 1.
6 HI 8072 HI 8082 1M KNO3 Electrolyte in FDA approved bottle, 4x30 mL 3.5M KCl Electrolyte in FDA approved bottle, 4x30 mL, for double junction electrodes ORP PRETREATMENT SOLUTIONS HI 7091L HI 7092L Reducing Pretreatment Solution, 460 mL Oxidizing Pretreatment Solution, 460 mL pH ELECTRODES All electrodes with part numbers ending in P are supplied with a BNC and pin connector and 1 m (3.3') cable, as shown below. HI 1043P; Use: strong acid/alkali.
HI 1083P; Use: biotechnology, micro titration. Glass-body, micro, Viscolene, nonrefillable, combination pH electrode 12 mm 0.5" 5 mm 0.2" 3 mm 0.12" 3.0 mm DIA 0.12" HI 1083 120 mm 4.7" ORP ELECTRODES HI 3131P; Use: titration. Glass-body, refillable, combination platinum ORP electrode 12 mm 0.5" HI 3131 150 mm 5.9" "S" VERSION Consult the Hanna General Catalog for more electrodes with BNC and pin connectors.
8 RECOMMENDATIONS FOR USERS Before using this product, make sure that it is entirely suitable for the environment in which it is used. Operation of this instrument in residential areas could cause unacceptable interferences to radio and TV equipment, requiring the operator to take all necessary steps to correct interferences. The glass bulb at the end of the pH electrode is sensitive to electrostatic discharges. Avoid touching this glass bulb at all times.
HANNA SALES AND TECHNICAL SERVICE Australia: Tel. (03) 9769.0666 Fax (03) 9769.0699 China: Tel. (10) 88570068 Fax (10) 88570060 Egypt: Tel. & Fax (02) 2758.683 Germany: Tel. (07851) 9129-0 Fax (07851) 9129-99 Greece: Tel. (210) 823.5192 Fax (210) 884.0210 Indonesia: Tel. (21) 4584.2941 Fax (21) 4584.2942 Japan: Tel. (03) 3258.9565 Fax (03) 3258.9567 Korea: Tel. (02) 2278.5147 Fax (02) 2264.1729 Malaysia: Tel. (603) 5638.9940 Fax (603) 5638.9829 Norway: Tel. (23) 3811.
