Instruction Manual HI 83203 Multiparameter Bench Photometer for Aquaculture This Instrument is in Compliance with the CE Directives www.hannainst.
Dear Customer, Thank you for choosing a Hanna product. Please read this instruction manual carefully before using the instrument. This manual will provide you with the necessary information for the correct use of the instrument. If you need additional technical information, do not hesitate to e-mail us at tech@hannainst.com. This instrument is in compliance with directives. TABLE OF CONTENTS PRELIMINARY EXAMINATION ............................................................................................
SPECIFICATIONS Life of the instrument Silicon Photocell 0 to 50°C (32 to 122°F); max 90% RH non-condensing Power Supply external 12 Vdc power adapter built-in rechargeable battery Dimensions 235 x 200 x 110 mm (9.2 x 7.87 x 4.33") Weight 0.9 Kg For specifications related to each method (e.g. range, precision, etc.) refer to the related measurement section. Light Life Light Detector Environment PRECISION AND ACCURACY Precision is how closely repeated measurements agree with each other.
FUNCTIONAL DESCRIPTION KEYPAD DESCRIPTION The keypad contains 8 direct keys and 3 functional keys with the following functions: Press to perform the function displayed above it. The functions are screen related. INSTRUMENT DESCRIPTION ESC Press to exit the current screen. Press to access the select method menu. Press to move up in a menu or a help screen, to increment a set value, to access second level functions.
(c) take out the syringe and clean the outside of the syringe tip. Be sure that no drops are hanging on the tip of the syringe, if so eliminate them. Then, keeping the syringe in vertical position above the cuvette, push the plunger down into the syringe until the lower edge of the seal is exactly on the 0.5 mL mark. Now the exact amount of 0.5 mL has been added to the cuvette, even if the tip still contains some solution.
• It is possible to take multiple readings in a row, but it is recommended to take a new zero reading for each sample and to use the same cuvette for zeroing and measurement when possible (for most precise results follow the measurement procedures carefully). • Discard the sample immediately after the reading is taken, or the glass might become permanently stained. • All the reaction times reported in this manual are at 20°C (68°F).
• After the desired method was selected, follow the measurement described in the related section. • Before performing a test you should read all the instructions carefully. DATA MANAGEMENT The instrument features a data log function to help you keep track of all your analysis. The data log can hold 200 individual measurements. Storing, viewing and deleting the data is possible using LOG and RCL keys.. Storing data: You can store only a valid measurement.
Date / Time This option is used to set the instrument’s date and time. Press Modify functional key to change the date/time. Press the WX functional keys to highlight the value to be modified (year, month, day, hour, minute or second). Use the keys to change the value. Press Accept functional key to confirm or ESC to return to the setup without saving the new date or time. Time format Option: AM/PM or 24 hour. Press the functional key to select the desired time format.
AMMONIA MEDIUM RANGE SPECIFICATIONS Range Resolution Accuracy Typical EMC Deviation Light Source Method 0.00 to 10.00 mg/L 0.01 mg/L ±0.05 mg/L ±5% of reading ±0.01 mg/L Tungsten lamp with narrow band interference filter @ 420 nm Adaptation of the ASTM Manual of Water and Environmental Technology, D1426-92, Nessler method. The reaction between ammonia and reagents causes a yellow tint in the sample.
• Remove the cuvette. AMMONIA LOW RANGE SPECIFICATIONS Range Resolution Accuracy Typical EMC Deviation Light Source Method • Add 4 drops of HI 93700A-0 First reagent (6 drops for seawater analysis). Replace the cap and mix the solution. 0.00 to 3.00 mg/L 0.01 mg/L ±0.04 mg/L ±4% of reading ±0.01 mg/L Tungsten lamp with narrow band interference filter @ 420 nm Adaptation of the ASTM Manual of Water and Environmental Technology, D1426-92, Nessler method.
• Press ZERO key. The meter will show “-0.0-” when the meter is zeroed and ready for measurement. FREE CHLORINE CHLORINE FREE SPECIFICATIONS Range Resolution Accuracy Typical EMC Deviation Light Source Method 0.00 to 2.50 mg/L 0.01 mg/L ±0.03 mg/L ±3% of reading ±0.01 mg/L • Remove the cuvette. Tungsten lamp with narrow band interference filter @ 525 nm Adaptation of the EPA DPD method 330.5. The reaction between free chlorine and the DPD reagent causes a pink tint in the sample.
TOTAL CHLORINE • Reinsert the cuvette into the instrument. SPECIFICATIONS • Press READ to start the reading. The instrument displays the results in mg/L of free chlorine. Range Resolution Accuracy Typical EMC Deviation Light Source Method 0.00 to 3.50 mg/L 0.01 mg/L ±0.03 mg/L ±3% of reading ±0.01 mg/L Tungsten lamp with narrow band interference filter @ 525 nm Adaptation of the EPA DPD method 330.5. The reaction between the chlorine and the DPD reagent causes a pink tint in the sample.
• Press ZERO key. The meter will show “-0.0-” when the meter is zeroed and ready for measurement. • Remove the cuvette. Powder reagents procedure • Add 1 packet of HI 93711-0 DPD reagent. Replace the cap and shake gently for 20 seconds (or 2 minutes for seawater analysis). • Press TIMER and the display will show the countdown prior to the measurement or, alternatively, wait for 2 minutes and 30 seconds and press READ. When the timer ends the meter will perform the reading.
• Remove the cuvette. COPPER HIGH RANGE • Add the content of one packet of HI 93702-0 Bicinchoninate. Replace the cap and shake gently for about 15 seconds. SPECIFICATIONS Range Resolution Accuracy Typical EMC Deviation Light Source Method 0.00 to 5.00 mg/L 0.01 mg/L ±0.02 mg/L ±4% of reading ±0.01 mg/L Tungsten lamp with narrow band interference filter @ 575 nm Adaptation of the EPA method. The reaction between copper and the bicinchoninate reagent causes a purple tint in the sample.
• Remove the cuvette. COPPER LOW RANGE • Add the content of one packet of HI 93747-0 Bicinchoninate. Replace the cap and shake gently for about 15 seconds. SPECIFICATIONS Range Resolution Accuracy Typical EMC Deviation Light Source Method 0 to 1000 μg/L 1 μg/L ±10 μg/L ±5% of reading ±1 μg/L Tungsten lamp with narrow band interference filter @ 575 nm Adaptation of the EPA method. The reaction between copper and the bicinchoninate reagent causes a purple tint in the sample.
NITRATE SPECIFICATIONS Range Resolution Accuracy Typical EMC Deviation Light Source Method 0.0 to 30.0 mg/L 0.1 mg/L ±0.5 mg/L ±10% of reading ±0.1 mg/L Tungsten lamp with narrow band interference filter @ 525 nm Adaptation of the cadmium reduction method. The reaction between nitrate and the reagent causes an amber tint in the sample. REQUIRED REAGENTS Code HI 93728-0 Description Powder reagent Quantity 1 packet • Replace the cap and immediately shake vigorously up and down for exactly 10 seconds.
NITRITE HIGH RANGE • Reinsert the cuvette into the instrument. SPECIFICATIONS Range Resolution Accuracy Typical EMC Deviation Light Source Method 0 to 150 mg/L 1 mg/L ±4 mg/L ±4% of reading ±1 mg/L Tungsten lamp with narrow band interference filter @ 575 nm Adaptation of the Ferrous Sulfate method. The reaction between nitrite and the reagent causes a greenish-brown tint in the sample.
NITRITE LOW RANGE • Reinsert the cuvette into the instrument. SPECIFICATIONS Range Resolution Accuracy Typical EMC Deviation Light Source Method 0.00 to 0.35 mg/L 0.01 mg/L ±0.02 mg/L ±4% of reading ±0.01 mg/L Tungsten lamp with narrow band interference filter @ 525 nm Adaptation of the EPA Diazotization method 354.1. The reaction between nitrite and the reagent causes a pink tint in the sample.
DISSOLVED OXYGEN • Let the sample stand and the flocculent agent will start to settle. SPECIFICATIONS Range Resolution Accuracy Typical EMC Deviation Light Source Method • After approximately 2 minutes, when the upper half of the bottle becomes limpid, add 10 drops of HI 93732C-0. 0.0 to 10.0 mg/L 0.1 mg/L ±0.4 mg/L ±3% of reading ± 0.
pH • Reinsert the cuvette into the instrument. SPECIFICATIONS Range Resolution Accuracy Typical EMC Deviation Light Source Method 6.5 to 8.5 pH 0.1 pH ±0.1 pH ±0.1 pH • Press the READ key to start the reading. The instrument displays the pH value. Tungsten lamp with narrow band interference filter @ 525 nm Adaptation of the Phenol Red method. The reaction with the reagent causes a yellow to red tint in the sample.
• Add the content of one packet of HI 93717B-0 Phosphate HR Reagent B to the cuvette. Replace the cap and shake gently until completely dissolved. PHOSPHATE HIGH RANGE SPECIFICATIONS Range Resolution Accuracy Typical EMC Dev. Light Source Method 0.0 to 30.0 mg/L 0.1 mg/L ±1 mg/L ±4% of reading ±0.1 mg/L Tungsten lamp with narrow band interference filter @ 525 nm Adaptation of the Standard Methods for the Examination of Water and Wastewater, 18th edition, Amino Acid method.
PHOSPHATE LOW RANGE • Reinsert the cuvette into the instrument. SPECIFICATIONS Range Resolution Accuracy Typical EMC Dev. Light Source Method 0.00 to 2.50 mg/L 0.01 mg/L ±0.04 mg/L ±4% of reading ±0.01 mg/L Tungsten lamp with narrow band interference filter @ 610 nm Adaptation of the Ascorbic Acid method. The reaction between phosphate and the reagent causes a blue tint in the sample.
ERRORS AND WARNINGS The instrument shows clear warning messages when erroneous conditions appear and when measured values are outside the expected range. These messages are described below. DATA MANAGEMENT The analyzed data can be managed using Hanna’s product HI 92000, Windows® Compatible Software. No Light: The light source is not functioning properly. Light Leak: There is an excess amount of ambient light reaching the detector. Inverted cuvettes: The sample and the zero cuvettes are inverted.
ACCESSORIES WARRANTY All Hanna Instruments meters are warranted for two years against defects in workmanship and materials when used for its intended purpose and maintained according to the instructions. This warranty is limited to repair or replacement free of charge. Damages due to accident, misuse, tampering or lack of prescribed maintenance are not covered. If service is required, contact your dealer.
SALES AND TECHNICAL SERVICE CONTACTS Australia: Tel. (03) 9769.0666 • Fax (03) 9769.0699 e-mail: hannains@hannainst.com.au China: Tel. (10) 88570068 • Fax (10) 88570060 e-mail: hannachina@vip.sina.com Egypt: Tel. & Fax (02) 2758.683 e-mail: hannaegypt@go.com.eg Germany: Tel. (07851) 9129-0 • Fax (07851) 9129-99 e-mail: hannager@aol.com Greece: Tel. (210) 823.5192 • Fax (210) 884.0210 e-mail: hannagr@otenet.gr Indonesia: Tel. (21) 4584.2941 • Fax (21) 4584.2942 e-mail: transit@dnet.net.
