Instruction Manual HI 83203 Multiparameter Bench Photometer for Aquaculture www.hannainst.
Dear Customer, Thank you for choosing a Hanna product. Please read this instruction manual carefully before using the instrument. This manual will provide you with the necessary information for the correct use of the instrument. If you need additional technical information, do not hesitate to e-mail us at tech@hannainst.com. TABLE OF CONTENTS PRELIMINARY EXAMINATION ..............................................................................................................................................
PRELIMINARY EXAMINATION Please examine this product carefully. Make sure that the instrument is not damaged. If any damage occurred during shipment, please notify your local Hanna Office. Each Meter is supplied complete with: • Two Sample Cuvettes and Caps • Cloth for wiping cuvettes (1 pcs) • 60 mL glass bottle for dissolved oxygen analysis (1 pcs) • Scissors • AC/DC Power Adapter • Instruction Manual Note: Save all packing material until you are sure that the instrument works correctly.
SPECIFICATIONS Life of the instrument Silicon Photocell 0 to 50°C (32 to 122°F); max 90% RH non-condensing Power Supply external 12 Vdc power adapter built-in rechargeable battery Dimensions 235 x 200 x 110 mm (9.2 x 7.87 x 4.33") Weight 0.9 Kg For specifications related to each method (e.g. range, resolution, etc.) refer to the related measurement section. Light Life Light Detector Environment PRECISION AND ACCURACY Precision is how closely repeated measurements agree with each other.
Where: -log I/Io= Absorbance (A) Io = intensity of incident light beam I = intensity of light beam after absorption ελ = molar extinction coefficient at wavelength λ c = molar concentration of the substance d = optical path through the substance Therefore, the concentration "c" can be calculated from the absorbance of the substance as the other factors are known.
FUNCTIONAL DESCRIPTION INSTRUMENT DESCRIPTION 1) 2) 3) 4) 5) 6) 7) 8) Open Cuvette Lid Indexing mark Cuvette point Liquid Crystal Display (LCD) Splash proof keypad ON/OFF power switch Power input connector USB connector 6
KEYPAD DESCRIPTION The keypad contains 8 direct keys and 3 functional keys with the following functions: Press to perform the function displayed above it on the LCD. ESC Press to exit the current screen. Press to access the select method menu. Press to move up in a menu or a help screen, to increment a set value, to access second level functions. Press to move down in a menu or a help screen, to decrement a set value, to access second level functions. Press to log the current reading.
(c) take out the syringe and clean the outside of the syringe tip. Be sure that no drops are hanging on the tip of the syringe, if so eliminate them. Then, keeping the syringe in vertical position above the cuvette, push the plunger down into the syringe until the lower edge of the seal is exactly on the 0.5 mL mark. Now the exact amount of 0.5 mL has been added to the cuvette, even if the tip still contains some solution.
USING CUVETTES • Proper mixing is very important for reproducibility of the measurements. The right way of mixing a cuvette is specified for each method in the related chapter. (a) invert the cuvette a couple of times or for a specified time: hold the cuvette in the vertical position. Turn the cuvette upside-down and wait for all of the solution to flow to the cap end, then return the cuvette to the upright vertical position and wait for all of the solution to flow to the cuvette bottom.
• In order to avoid reagent leaking and to obtain more accurate measurements, close the cuvette first with the supplied HDPE plastic stopper and then the black cap. • Each time the cuvette is used, the cap must be tightened to the same degree. • Whenever the cuvette is placed into the measurement cell, it must be dry outside, and free of fingerprints, oil or dirt. Wipe it thoroughly with HI 731318 or a lint-free cloth prior to insertion.
METHOD REFERENCE TABLE Method 1 2 3 4 5 6 7 Method description Ammonia MR Ammonia LR Free Chlorine Total Chlorine Copper HR Copper LR Nitrate Page Method 16 18 20 23 26 28 30 8 9 10 11 12 13 Method description Nitrite HR Nitrite LR Dissolved Oxygen pH Phosphate HR Phosphate LR Page 32 34 36 38 40 42 OPERATIONAL GUIDE POWER CONNECTION AND BATTERY MANAGEMENT The meter can be powered from an AC/DC adapter (included) or from the built-in rechargeable battery.
METHOD SELECTION • Turn the instrument ON via the ON/OFF power switch. • The meter will perform an autodiagnostic test. During this test, the Hanna Instrument logo will appear on the LCD. After 5 seconds, if the test was successful, the last method used will appear on the display. • In order to select the desired method press the METHOD key and a screen with the available methods will appear. • Press the keys to highlight the desired method. Press Select.
CHEMICAL FORM Chemical form conversion factors are pre-programmed into the instrument and are method specific. In order to view the displayed result in the desired chemical form press or to access the second level functions and then press the Chem Frm key to toggle between the available chemical forms for the selected method. SETUP In the Setup mode the instrument’s parameters can be changed.
Date / Time This option is used to set the instrument’s date and time. Press the Modify key to change the date/time. Press the W X functional keys to highlight the value to be modified (year, month, day, hour, minute or second). Use the keys to change the value. Press the Accept key to confirm or ESC to return to the setup without saving the new date or time. Time format Option: AM/PM or 24 hour. Press the functional key to select the desired time format.
Instrument ID Option: 0 to 9999. This option is used to set the instrument’s ID (identification number). The instrument ID is used while exchanging data with a PC. Press the Modify key to access the instrument ID screen. Press the keys in order to set the desired value. Press the Accept key to confirm the value or ESC to return to the setup menu without saving the new value. Meter information Press the Select key to view the instrument model, firmware version, language version and instrument serial number.
AMMONIA MEDIUM RANGE SPECIFICATIONS Range Resolution Accuracy Typical EMC Deviation Light Source Method 0.00 to 10.00 mg/L 0.01 mg/L ±0.05 mg/L ±5% of reading at 25 °C ±0.01 mg/L Tungsten lamp with narrow band interference filter @ 420 nm Adaptation of the ASTM Manual of Water and Environmental Technology, D1426-92, Nessler method. The reaction between ammonia and reagents causes a yellow tint in the sample.
• Remove the cuvette. • Add 4 drops of HI 93715A-0 First reagent (6 drops for seawater analysis). Replace the cap and mix the solution. • Add 4 drops of HI 93715B-0 Second reagent (10 drops for seawater analysis). Replace the cap and mix the solution. • Reinsert the cuvette into the instrument. • Press Timer and the display will show the countdown prior to the measurement or, alternatively, wait for 3 minutes and 30 seconds and press Read. When the timer ends the meter will perform the reading.
AMMONIA LOW RANGE SPECIFICATIONS Range Resolution Accuracy Typical EMC Deviation Light Source Method 0.00 to 3.00 mg/L 0.01 mg/L ±0.04 mg/L ±4% of reading at 25 °C ±0.01 mg/L Tungsten lamp with narrow band interference filter @ 420 nm Adaptation of the ASTM Manual of Water and Environmental Technology, D1426-92, Nessler method. The reaction between ammonia and reagents causes a yellow tint in the sample.
• Remove the cuvette. • Add 4 drops of HI 93700A-0 First reagent (6 drops for seawater analysis). Replace the cap and mix the solution. • Add 4 drops of HI 93700B-0 Second reagent (10 drops for seawater analysis). Replace the cap and mix the solution. • Reinsert the cuvette into the instrument. • Press Timer and the display will show the countdown prior to the measurement or, alternatively, wait for 3 minutes and 30 seconds and press Read. When the timer ends the meter will perform the reading.
FREE CHLORINE CHLORINE FREE SPECIFICATIONS Range Resolution Accuracy Typical EMC Deviation Light Source Method 0.00 to 2.50 mg/L 0.01 mg/L ±0.03 mg/L ±3% of reading at 25 °C ±0.01 mg/L Tungsten lamp with narrow band interference filter @ 525 nm Adaptation of the EPA DPD method 330.5. The reaction between free chlorine and the DPD reagent causes a pink tint in the sample.
• Press the Zero key. The meter will show “-0.0-” when the meter is zeroed and ready for measurement. • Remove the cuvette. Powder reagents procedure • Add the content of one packet of HI 93701-0 DPD reagent. Replace the cap and shake gently for 20 seconds (or 2 minutes for seawater analysis). • Reinsert the cuvette into the instrument. • Press Timer and the display will show the countdown prior to the measurement or, alternatively, wait for 1 minute and press Read.
• Reinsert the cuvette into the instrument. • Press Read to start the reading. The instrument displays the results in mg/L of free chlorine. INTERFERENCES Interference may be caused by: Bromine, Iodine, Ozone, Oxidized forms of Chromium and Manganese. In case of water with hardness greater than 500 mg/L CaCO3, shake the sample for approximately 2 minutes after adding the powder reagent.
TOTAL CHLORINE SPECIFICATIONS Range Resolution Accuracy Typical EMC Deviation Light Source Method 0.00 to 3.50 mg/L 0.01 mg/L ±0.03 mg/L ±3% of reading at 25 °C ±0.01 mg/L Tungsten lamp with narrow band interference filter @ 525 nm Adaptation of the EPA DPD method 330.5. The reaction between the chlorine and the DPD reagent causes a pink tint in the sample.
• Press the Zero key. The meter will show “-0.0-” when the meter is zeroed and ready for measurement. • Remove the cuvette. Powder reagents procedure • Add 1 packet of HI 93711-0 DPD reagent. Replace the cap and shake gently for 20 seconds (or 2 minutes for seawater analysis). • Reinsert the cuvette into the instrument. • Press Timer and the display will show the countdown prior to the measurement or, alternatively, wait for 2 minutes and 30 seconds and press Read.
• Press Timer and the display will show the countdown prior to the measurement or, alternatively, wait for 2 minutes and 30 seconds and press Read. When the timer ends the meter will perform the reading. • The instrument displays the results in mg/L of total chlorine. Note: free and total chlorine have to be measured separately with fresh unreacted samples following the related procedure if both values are requested.
COPPER HIGH RANGE SPECIFICATIONS Range Resolution Accuracy Typical EMC Deviation Light Source Method 0.00 to 5.00 mg/L 0.01 mg/L ±0.02 mg/L ±4% of reading at 25 °C ±0.01 mg/L Tungsten lamp with narrow band interference filter @ 575 nm Adaptation of the EPA method. The reaction between copper and the bicinchoninate reagent causes a purple tint in the sample.
• Remove the cuvette. • Add the content of one packet of HI 93702-0 Bicinchoninate. Replace the cap and shake gently for about 15 seconds. • Reinsert the cuvette into the instrument. • Press Timer and the display will show the countdown prior to the measurement or, alternatively, wait for 45 seconds and press Read. When the timer ends the meter will perform the reading. • The instrument displays the results in mg/L of copper.
COPPER LOW RANGE SPECIFICATIONS Range Resolution Accuracy Typical EMC Deviation Light Source Method 0 to 1000 μg/L 1 μg/L ±10 μg/L ±5% of reading at 25 °C ±1 μg/L Tungsten lamp with narrow band interference filter @ 575 nm Adaptation of the EPA method. The reaction between copper and the bicinchoninate reagent causes a purple tint in the sample.
• Remove the cuvette. • Add the content of one packet of HI 95747-0 Bicinchoninate. Replace the cap and shake gently for about 15 seconds. • Reinsert the cuvette into the instrument. • Press Timer and the display will show the countdown prior to the measurement or, alternatively, wait for 45 seconds and press Read. When the timer ends the meter will perform the reading. • The instrument displays the results in μg/L of copper.
NITRATE SPECIFICATIONS Range Resolution Accuracy Typical EMC Deviation Light Source Method 0.0 to 30.0 mg/L 0.1 mg/L ±0.5 mg/L ±10% of reading at 25 °C ±0.1 mg/L Tungsten lamp with narrow band interference filter @ 525 nm Adaptation of the cadmium reduction method. The reaction between nitrate and the reagent causes an amber tint in the sample.
• Replace the cap and immediately shake vigorously up and down for exactly 10 seconds. Continue to mix by inverting the cuvette gently for 50 seconds, while taking care not to induce air bubbles. Powder will not completely dissolve. Time and way of shaking could sensitively affect the measurement. • Reinsert the cuvette into the instrument, taking care not to shake it.
NITRITE HIGH RANGE SPECIFICATIONS Range Resolution Accuracy Typical EMC Deviation Light Source Method 0 to 150 mg/L 1 mg/L ±4 mg/L ±4% of reading at 25 °C ±1 mg/L Tungsten lamp with narrow band interference filter @ 575 nm Adaptation of the Ferrous Sulfate method. The reaction between nitrite and the reagent causes a greenish-brown tint in the sample.
• Add the content of one packet of HI 93708-0 reagent. Replace the cap and shake gently until completely dissolved. • Reinsert the cuvette into the instrument. • Press Timer and the display will show the countdown prior to the measurement or, alternatively, wait for 10 minutes and press Read. When the timer ends the meter will perform the reading. The instrument displays concentration in mg/L of nitrite. • Press or to access the second level functions.
NITRITE LOW RANGE SPECIFICATIONS Range Resolution Accuracy Typical EMC Deviation Light Source Method 0.00 to 1.15 mg/L 0.01 mg/L ±0.06 mg/L ±4% of reading at 25 °C ±0.01 mg/L Tungsten lamp with narrow band interference filter @ 525 nm Adaptation of the EPA Diazotization method 354.1. The reaction between nitrite and the reagent causes a pink tint in the sample.
• Reinsert the cuvette into the instrument. • Press Timer and the display will show the countdown prior to the measurement or, alternatively, wait for 6 minutes and press Read. When the timer ends the meter will perform the reading. The instrument displays concentration in mg/L of nitrite. • Press or to access the second level functions. • Press the Chem Frm key to convert the result in mg/L of nitrogen-nitrite (NO2¯-N) and sodium nitrite (NaNO2). • Press or to return to the measurement screen.
DISSOLVED OXYGEN SPECIFICATIONS Range Resolution Accuracy Typical EMC Deviation Light Source Method 0.0 to 10.0 mg/L 0.1 mg/L ±0.4 mg/L ±3% of reading at 25 °C ± 0.1 mg/L Tungsten lamp with narrow band interference filter @ 420 nm Adaptation of the Standard Methods for the Examination of Water and Wastewater, 18th edition, Azide modified Winkler method. The reaction between dissolved oxygen and the reagents causes a yellow tint in the sample.
• Let the sample stand and the flocculent agent will start to settle. • After approximately 2 minutes, when the upper half of the bottle becomes limpid, add 10 drops of HI 93732C-0. • Replace the cap and invert the bottle until the settled flocculent dissolves completely. The sample is ready for measurement when it is yellow and completely limpid. 10 mL • Fill the cuvette up to the mark with 10 mL of the unreacted (original) sample, and replace the cap. This is the blank.
pH SPECIFICATIONS Range Resolution Accuracy Typical EMC Deviation Light Source Method 6.5 to 8.5 pH 0.1 pH ±0.1 pH at 25 °C ±0.1 pH Tungsten lamp with narrow band interference filter @ 525 nm Adaptation of the Phenol Red method. The reaction with the reagent causes a yellow to red tint in the sample.
• Remove the cuvette and add 5 drops of HI 93710-0 Phenol Red Indicator. Replace the cap and mix the solution. • Reinsert the cuvette into the instrument. • Press the Read key to start the reading. The instrument displays the pH value.
PHOSPHATE HIGH RANGE SPECIFICATIONS Range Resolution Accuracy Typical EMC Dev. Light Source Method 0.0 to 30.0 mg/L 0.1 mg/L ±1 mg/L ±4% of reading at 25 °C ±0.1 mg/L Tungsten lamp with narrow band interference filter @ 525 nm Adaptation of the Standard Methods for the Examination of Water and Wastewater, 18th edition, Amino Acid method. The reaction between phosphate and reagents causes a blue tint in the sample.
• Add the content of one packet of HI 93717B-0 Phosphate HR Reagent B to the cuvette. Replace the cap and shake gently until completely dissolved. • Reinsert the cuvette into the instrument. • Press Timer and the display will show the countdown prior to the measurement or, alternatively, wait for 5 minutes and press Read. When the timer ends the meter will perform the reading. The instrument displays the results in mg/L of phosphate (PO43¯). • Press or to access the second level functions.
PHOSPHATE LOW RANGE SPECIFICATIONS Range Resolution Accuracy Typical EMC Dev. Light Source Method 0.00 to 2.50 mg/L 0.01 mg/L ±0.04 mg/L ±4% of reading at 25 °C ±0.01 mg/L Tungsten lamp with narrow band interference filter @ 610 nm Adaptation of the Ascorbic Acid method. The reaction between phosphate and the reagent causes a blue tint in the sample.
• Reinsert the cuvette into the instrument. • Press Timer and the display will show the countdown prior to the measurement or, alternatively, wait for 3 minutes and press Read. When the timer ends the meter will perform the reading. The instrument displays concentration in mg/L of phosphate (PO43¯). • Press or to access the second level functions. • Press the Chem Frm key to convert the result in mg/L of phosphorus (P) and phosphorus pentoxide (P2O5). • Press or to return to the measurement screen.
ERRORS AND WARNINGS The instrument shows clear warning messages when erroneous conditions appear and when measured values are outside the expected range. These messages are described below. No Light: The light source is not functioning properly. Light Leak: There is an excess amount of ambient light reaching the detector. Inverted cuvettes: The sample and the zero cuvettes are inverted. Battery Low: The battery capacity is lower than 10%. Light Low: The instrument cannot adjust the light level.
DATA MANAGEMENT The analyzed data can be managed using Hanna’s product HI 92000, Windows ® Compatible Software. STANDARD METHODS Description Ammonia MR Ammonia LR Chlorine, Free Chlorine, Total Copper HR Copper LR Nitrate Nitrite HR Nitrite LR Oxygen, Dissolved pH Phosphate HR Phosphate LR Range 0.00 to 10.00 mg / L 0.00 to 3.00 mg / L 0.00 to 2.50 mg / L 0.00 to 3.50 mg / L 0.00 to 5.00 mg/L 0 to 1000 μg/L 0.0 to 30.0 mg / L 0 to 150 mg/L 0.00 to 1.15 mg/L 0.0 to 10.0 mg/L 6.5 to 8.5 pH 0.0 to 30.
ACCESSORIES REAGENT SETS HI 93700-01 100 ammonia LR tests HI 93700-03 300 ammonia LR tests HI 93701-01 100 free chlorine tests (powder) HI 93701-03 300 free chlorine tests (powder) HI 93701-F 300 free chlorine tests (liquid) HI 93701-T 300 total chlorine tests (liquid) HI 93702-01 100 copper HR tests HI 93702-03 300 copper HR tests HI 93707-01 100 nitrite LR tests HI 93707-03 300 nitrite LR tests HI 93708-01 100 nitrite HR tests HI 93708-03 300 nitrite HR tests HI 93710-01 100 pH tests HI 93710-03 300 pH te
WARRANTY All Hanna Instruments meters are warranted for two years against defects in workmanship and materials when used for its intended purpose and maintained according to the instructions. This warranty is limited to repair or replacement free of charge. Damages due to accident, misuse, tampering or lack of prescribed maintenance are not covered. If service is required, contact your dealer. If under warranty, report the model number, date of purchase, serial number and the nature of the failure.
Hanna Instruments Inc. Highland Industrial Park 584 Park East Drive Woonsocket, RI 02895 USA Technical Support for Customers Tel. (800) 426 6287 Fax (401) 765 7575 E-mail tech@hannainst.com www.hannainst.
