Instruction Manual HI 83214 Multiparameter Bench Photometer for Wastewater Treatment www.hannainst.
Dear Customer, Thank you for choosing a Hanna product. Please read this instruction manual carefully before using the meter. This manual will provide you with the necessary information for the correct use of the instrument. If you need additional technical information, do not hesitate to e-mail us at tech@hannainst.com. TABLE OF CONTENTS PRELIMINARY EXAMINATION .............................................................................................................................................
PRELIMINARY EXAMINATION Please examine this product carefully. Make sure that the instrument is not damaged. If any damage occurred during shipment, please notify your Local Hanna Office. Each Meter is supplied complete with: • Ten Sample Vials • Cloth for wiping vials (1 pcs.) • Scissors • Instruction Manual Note: Save all packing material until you are sure that the instrument works correctly. Any defective item must be returned in its original packing with the supplied accessories.
SPECIFICATIONS Light Life Light Detector Environment Life of the instrument Silicon Photocell 0 to 50 °C (32 to 122 °F); max 90% RH non-condensing Power Supply External 12 Vdc power adaptor Dimensions 235 x 212 x 143 mm (9.2 x 8.34 x 5.62") Weight 1.5 Kg (3.3 lb) For specifications related to each method (e.g. range, resolution etc.) refer to the related measurement section. PRECISION AND ACCURACY Precision is how closely repeated measurements agree with each other.
Where: -log I/Io=Absorbance (A) Io =intensity of incident light beam I =intensity of light beam after absorption ελ =molar extinction coefficient at wavelength λ c =molar concentration of the substance d =optical path through the substance Therefore, the concentration "c" can be calculated from the absorbance of the substance as the other factors are known.
FUNCTIONAL DESCRIPTION INSTRUMENT DESCRIPTION 1) 2) 3) 4) 5) 6) 7) Vial holder Liquid Crystal Display (LCD) Keypad Bottom lid USB connector ON/OFF power switch Power supply connector 6
KEYPAD DESCRIPTION The keypad contains 8 direct keys and 3 functional keys with the following functions: Press to perform the function displayed above it on the LCD. ESC Press to exit the current screen. Press to access the method menu. Press to move up in a menu or a help screen, to increment a set value, or to access second level functions. Press to move down in a menu or a help screen, to decrement a set value, or to access second level functions. Press to log the current reading.
TIPS FOR AN ACCURATE MEASUREMENT The instructions listed below should be carefully followed during testing to ensure most accurate results.
2. In order to measure exactly 0.5 mL of reagent with the 1 mL syringe: (a) push the plunger completely into the syringe and insert the tip into the solution (b) pull the plunger up until the lower edge of the seal is exactly on the 0.0 mL mark (c) take out the syringe and clean the outside of the syringe tip. Be sure that no drops are hanging on the tip of the syringe, if so eliminate them.
• Proper use of the powder reagent packet: (a) use scissors to open the powder packet (b) push the edges of the packet to form a spout (c) pour out the content of the packet (a) (b) (c) USING VIALS • Never insert hot vials in the instrument, the vial holder may become damaged. • In order to avoid reagent leaking and to obtain most accurate results, it is recommended to close the vial tightly with the supplied cap after addition of reagents or sample.
(b) shake the vial: move the vial up and down. The movement may be gentle or vigorous. This mixing technique is indicated with “shake gently” or “shake vigorously” and one of the following icons: shake gently shake vigorously Pay attention to push the cuvette completely down in the holder. • Whenever the vial is placed into the measurement cell, it must be dry on the outside, and completely free of fingerprints, oil or dirt. Wipe it thoroughly with HI 731318 or a lint-free cloth prior to insertion.
DIGESTION • Some analytical methods require digestion of the sample. Use the HI 839800 test tube heater for digestion. The optional HI 740217 safety shield is strongly recommended. For correct use of the reactor follow the Reactor Instruction Manual. At the end of the digestion period the vials are still hot, allow the vials to cool to room temperature in the optional HI 740216 test tube cooling rack. REAGENT BLANK CORRECTION • Some methods require a “reagent blank correction”.
METHOD REFERENCE TABLE Method Method reagent set code Method description Page 1 2 HI 93764A-25 HI 93764B-25 Ammonia LR Ammonia HR 20 22 3 HI 93701-01 HI 93701-03 Chlorine, Free 24 4 HI 93711-01 HI 93711-03 Chlorine, Total 26 5 6 HI 93766-50 HI 93767A-50 Nitrate Nitrogen, Total LR 28 30 7 HI 93767B-50 Nitrogen, Total HR 35 8 9 HI 93754A-25 HI 93754B-25 Oxygen Demand, Chemical (COD) LR Oxygen Demand, Chemical (COD) MR 40 43 10 HI 93754C-25 Oxygen Demand, Chemical (COD) HR 46 1
VIALS IDENTIFICATION Vials related to different parameters can be distinguished by means of the letter printed on the vial (beside the Hanna logo) and the color of the printing: Method reagent set code HI 93764A-25 HI 93764B-25 HI 93701-01 HI 93701-03 HI 93711-01 HI 93711-03 HI 93766-50 HI 93767A-50 Method description Ammonia LR Ammonia HR Chlorine, Free Code specific letter on the label A LR A HR (no letter) Label rectangle color white green white Chlorine, Total (no letter) Nitrate Nitrogen, Total
OPERATIONAL GUIDE POWER CONNECTION Connect the AC/DC adaptor that is supplied with the instrument. Note: Always turn the meter off before unplugging it to ensure no data is lost. METHOD SELECTION • Turn the instrument ON via the ON/OFF power switch. • The meter will perform an autodiagnostic test. During this test, the Hanna Instrument logo will appear on the LCD. After 5 seconds, if the test was successful, the last method used will appear on the display.
DATA MANAGEMENT You can keep track of your results using the instrument’s data log function. Up to 200 individual measurements can be stored in the data log. Storing, viewing and deleting the data is possible using LOG, RCL, t or s keys.. Storing data: You can store only a valid measurement. To store a valid measurement press LOG. The measurement will be stored with date and time stamps. Viewing and deleting: You can view and delete the data log by pressing the RCL key.
SETUP In the Setup mode the instrument’s parameters can be changed. Some parameters affect the measuring sequence and others are general parameters that change the behavior or appearance of the instrument. Press SETUP to enter the setup mode. Press ESC or SETUP to return to the main screen. A list of setup parameters will be displayed with currently configured settings. Press HELP for additional information.
Date / Time This option is used to set the instrument’s date and time. Press the Modify key to change the date/time. Press the ⊳ u functional keys to highlight the value to be modified (year, month, day, hour, minute or second). Use the s t keys to change the value. Press the Accept key to confirm or ESC to return to the setup without saving the new date or time. Time format Option: AM/PM or 24 hour. Press the functional key to select the desired time format.
Beeper Option: Enable or Disable. When enabled, a short beep is heard every time a key is pressed. A long beep alert sounds when the pressed key is not active or an error condition is detected. Press the functional key to enable/disable the beeper. Instrument ID Option: 0000 to 9999. This option is used to set the instrument’s ID (identification number). The instrument ID is used while exchanging data with a PC. Press the Modify key to access the instrument ID screen.
AMMONIA LOW RANGE SPECIFICATIONS Range Resolution Accuracy Light Source Method 0.00 to 3.00 mg/L (as NH3-N) 0.01 mg/L ±0.10 mg/L or ±5 % of reading @ 25 °C, whichever is greater Tungsten lamp with narrow band interference filter @ 420 nm Adaptation of the ASTM Manual of Water and Environmental Technology, D1426-92, Nessler method. The reaction between ammonia and reagents causes a yellow tint in the sample. REQUIRED REAGENTS Code Description Q.
• Press the Zero key. The display will show “-0.0-” when the meter is zeroed and ready for measurement. • Remove the vial. • Remove the cap and add 4 drops of HI 93764-0 Nessler Reagent. • Replace the cap tightly and mix by inverting the vial a couple of times. This is the sample. • Place the vial into the holder and push it completely down. • Press Timer and the display will show the countdown prior to the measurement and the message “Reaction Time”.
AMMONIA HIGH RANGE SPECIFICATIONS Range Resolution Accuracy Light Source Method 0 to 100 mg/L (as NH3-N) 1 mg/L ±1 mg/L or ±5 % of reading @ 25 °C, whichever is greater Tungsten lamp with narrow band interference filter @ 420 nm Adaptation of the ASTM Manual of Water and Environmental Technology, D1426-92, Nessler method. The reaction between ammonia and reagents causes a yellow tint in the sample. REQUIRED REAGENTS Code Description Q.ty/test Q.
• Press the Zero key. The display will show “-0.0-” when the meter is zeroed and ready for measurement. • Remove the vial. • Remove the cap and add 4 drops of HI 93764-0 Nessler Reagent. • Replace the cap tightly and mix by inverting the vial a couple of times. This is the sample. • Place the vial into the holder and push it completely down. • Press Timer and the display will show the countdown prior to the measurement and the message “Reaction Time”.
CHLORINE, FREE SPECIFICATIONS Range Resolution Accuracy Light Source Method 0.00 to 5.00 mg/L Cl2 0.01 mg/L ±0.03 mg/L or ±4 % of reading @ 25 °C, whichever is greater Tungsten lamp with narrow band interference filter @ 525 nm Adaptation of the EPA method 330.5 and Standard Methods for the Examination of Water and Wastewater, 20th edition, 4500-Cl G, DPD method. The reaction between free chlorine and the DPD reagent causes a pink tint in the sample.
• Remove the cap and add the content of one packet of HI 93701-0 Free Chlorine Reagent. • Replace the cap and shake gently to mix for about 20 seconds. This is the sample. • Place the vial into the holder and push it completely down. • Press Timer and the display will show the countdown prior to the measurement and the message “Reaction Time”. Alternatively, wait for 1 minute and press Read. The instrument will perform the reading. • The instrument displays concentration in mg/L of free chlorine.
CHLORINE, TOTAL SPECIFICATIONS Range Resolution Accuracy Light Source Method 0.00 to 5.00 mg/L Cl2 0.01 mg/L ±0.03 mg/L or ±4 % of reading @ 25 °C, whichever is greater Tungsten lamp with narrow band interference filter @ 525 nm Adaptation of the EPA method 330.5 and Standard Methods for the Examination of Water and Wastewater, 20th edition, 4500-Cl G, DPD method. The reaction between chlorine and the DPD reagent causes a pink tint in the sample.
• Remove the cap and add the content of one packet of HI 93711-0 Total Chlorine Reagent. • Replace the cap and shake gently to mix for about 20 seconds. This is the sample. • Place the vial into the holder and push it completely down. • Press Timer and the display will show the countdown prior to the measurement and the message “Reaction Time”. Alternatively, wait for 2 minutes and 30 seconds and press Read. The instrument will perform the reading.
NITRATE SPECIFICATIONS Range Resolution Accuracy Light Source Method 0.0 to 30.0 mg/L NO3--N 0.1 mg/L ±1.0 mg/L or ±5 % of reading @ 25 °C, whichever is greater Tungsten lamp with narrow band interference filter @ 420 nm Chromotropic acid method. The reaction between nitrate and the reagents causes a yellow tint in the sample. REQUIRED REAGENTS Code Description Q.ty/test HI 93766V-0* Reagent Vial 1 vial HI 93766-0 Nitrate Reagent 1 packet * Reagent Vial identification: N, white label.
• Press the Zero key. The display will show “-0.0-” when the meter is zeroed and ready for measurement. • Remove the vial. • Remove the cap and add the content of one packet of HI 93766-0 Nitrate Reagent. • Replace the cap tightly and invert the vial 10 times. This is the reacted sample. Note: The method is technique sensitive: to obtain most reproducible results it is strongly recommended to follow carefully the “invert” procedure described on page 10.
NITROGEN, TOTAL LOW RANGE SPECIFICATIONS Range Resolution Accuracy Light Source Method 0.0 to 25.0 mg/L N 0.1 mg/L ±1.0 mg/L or ±5 % of reading @ 25 °C, whichever is greater Tungsten lamp with narrow band interference filter @ 420 nm Chromotropic acid method. A persulfate digestion converts all forms of nitrogen to nitrate. Then the reaction between nitrate and the reagents causes a yellow tint in the sample. REQUIRED REAGENTS Code Description Q.ty/test Q.
MEASUREMENT PROCEDURE Before using the reagent kit carefully read all the instructions and the Material Safety Data Sheet (MSDS). Pay particular attention to all warnings, cautions and notes. Failure to do so may result in serious injury to the operator. Reagent Blank Correction: This method requires a reagent blank correction. A single blank vial may be used more than once; the blank vial is stable up to one week if stored in a dark place at room temperature.
• Replace the cap tightly and shake the vials vigorously for about 30 seconds until all the powder is completely dissolved. Sample Blank • Insert the vials into the reactor and heat them for 30 minutes at 150 °C. Note: To obtain most accurate results, it is strongly recommended to remove the vials from the reactor after 30 minutes. • At the end of the digestion period switch off the reactor, place the vials in the test tube rack and allow to cool to room temperature.
Sample Blank Blank Sample • Press Start to activate the 2 minutes timer or wait for 2 minutes (without shaking the vials) to allow the reaction to complete. • Remove the caps from two Reagent Vials (red label). ← red label → • Add exactly 2.0 mL of digested sample (sample vial) to one Reagent Vial (sample vial), and 2.0 mL of digested blank (blank vial) to the other Reagent Vial (blank vial), while keeping the vials at a 45-degree angle. • Replace the cap tightly and invert the vials 10 times.
• Press Start and the display will show the countdown prior to the measurement and the “Reaction Time” message. Alternatively, wait for 5 minutes and press Zero. • The display will show “-0.0-” when the meter is zeroed and ready for measurement. • Remove the blank vial. • Place the sample vial into the holder and push it completely down. • Press Read. The instrument will perform the reading. The instrument displays concentration in mg/L of total nitrogen (N).
NITROGEN, TOTAL HIGH RANGE SPECIFICATIONS Range Resolution Accuracy Light Source Method 10 to 150 mg/L N 1 mg/L ±3 mg/L or ±4 % of reading @ 25 °C, whichever is greater Tungsten lamp with narrow band interference filter @ 420 nm Chromotropic acid method. A persulfate digestion converts all forms of nitrogen to nitrate. Then the reaction between nitrate and the reagents causes a yellow tint in the sample. REQUIRED REAGENTS Code Description Q.ty/test Q.
MEASUREMENT PROCEDURE Before using the reagent kit carefully read all the instructions and the Material Safety Data Sheet (MSDS). Pay particular attention to all warnings, cautions and notes. Failure to do so may result in serious injury to the operator. Reagent Blank Correction: This method requires a reagent blank correction. A single blank vial may be used more than once; the blank vial is stable up to one week if stored in a dark place at room temperature.
• Replace the cap tightly and shake vigorously the vials for about 30 seconds until all the powder is completely dissolved. Sample Blank • Insert the vials into the reactor and heat them for 30 minutes at 105 °C. Note: To obtain most accurate results, it is strongly recommended to remove the vials from the reactor after 30 minutes. • At the end of the digestion place the vials in the test tube rack and allow to cool to room temperature. Warning: As the vials are still hot, be careful in handling them.
• Remove the cap from the vials and add the content of one packet of HI 93767-0 Total Nitrogen Reagent to each vial. Replace the cap tightly and shake the vials gently for 15 seconds. Sample Blank Blank Sample • Press Start to activate the 2 minutes timer or wait for 2 minutes (without shaking the vials) to allow the reaction to complete. • Remove the cap from two Reagent Vials (green label). ← green label → • Add exactly 2.
• Press Start and the display will show the countdown prior to the measurement and the ”Reaction Time” message. Alternatively, wait for 5 minutes and press Zero. • The instrument will perform a zero sequence and after a few seconds the display will show “-0.0-”. Now the meter is zeroed and ready for measurement. • Remove the blank vial and place the sample vial into the holder and push it completely down. • Press Read. The instrument will perform the reading.
OXYGEN DEMAND, CHEMICAL LOW RANGE SPECIFICATIONS Range Resolution Accuracy Light Source Method 0 to 150 mg/L COD 1 mg/L ±5 mg/L or ±5 % of reading @ 25 °C, whichever is greater Tungsten lamp with narrow band interference filter @ 420 nm Adaptation of the USEPA 410.4 approved method for the COD determination on surface waters and wastewaters. Oxidizable organic compounds reduce the dichromate ion (orange) to the chromic ion (green). The amount of remaining dichromate is determined.
• Choose a homogeneous sample. Samples containing settleable solids need to be homogenized with a blender. • Preheat the Hanna Reactor HI 839800 to 150 °C (302 °F). For correct use of the reactor follow Reactor Instruction Manual. The optional HI 740217 safety shield is strongly recommended. DO NOT USE AN OVEN OR MICROWAVE samples may leak and generate a corrosive and possibly explosive atmosphere. • Remove the caps from two Reagent Vials. ← red label → • Add exactly 2.
• Select Oxygen Demand, (COD) LR method following one of the procedures described in the “Method Selection” section (see page 15). • Place the blank vial into the holder and push it completely down. • Press the Zero key. The instrument will perform a zero sequence and after a few seconds the display will show “-0.0-”. Now the meter is zeroed and ready for measurement. • Remove the blank vial. • Place the sample vial into the holder and push it completely down. • Press Read.
OXYGEN DEMAND, CHEMICAL MEDIUM RANGE SPECIFICATIONS Range Resolution Accuracy Light Source Method 0 to 1500 mg/L COD 1 mg/L ±15 mg/L or ±4 % of reading @ 25 °C, whichever is greater Tungsten lamp with narrow band interference filter @ 610 nm Adaptation of the USEPA 410.4 approved method for the COD determination on surface waters and wastewaters. Oxidizable organic compounds reduce the dichromate ion (orange) to the chromic ion (green). The amount of chromic ion formed is determined.
• Choose a homogeneous sample. Samples containing settleable solids need to be homogenized with a blender. • Preheat the Hanna Reactor HI 839800 to 150 °C (302 °F). For correct use of the reactor follow Reactor Instruction Manual. Use of the optional HI 740217 safety shield is strongly recommended. DO NOT USE AN OVEN OR MICROWAVE samples may leak and generate a corrosive and possibly explosive atmosphere. • Remove the caps from two Reagent Vials. ← white label → • Add exactly 2.
• Select the Oxygen Demand, (COD) MR method following one of the procedures described in the “Method Selection” section (see page 15). • Place the blank vial into the holder and push it completely down. • Press the Zero key. The instrument will perform a zero sequence and after a few seconds the display will show “-0.0-”. Now the meter is zeroed and ready for measurement. • Remove the blank vial. • Place the sample vial into the holder and push it completely down. • Press Read.
OXYGEN DEMAND, CHEMICAL HIGH RANGE SPECIFICATIONS Range Resolution Accuracy Light Source Method 0 to 15000 mg/L COD 10 mg/L ±150 mg/L or ±3 % of reading @ 25 °C, whichever is greater Tungsten lamp with narrow band interference filter @ 610 nm Adaptation of the USEPA 410.4 approved method for the COD determination on surface waters and wastewaters. Oxidizable organic compounds reduce the dichromate ion (orange) to the chromic ion (green). The amount of chromic ion formed is determined.
• Choose a homogeneous sample. Samples containing settleable solids need to be homogenized with a blender. • Preheat the Hanna Reactor HI 839800 to 150 °C (302 °F). For correct use of the reactor follow Reactor Instruction Manual. Use of the optional HI 740217 safety shield is strongly recommended. DO NOT USE AN OVEN OR MICROWAVE samples may leak and generate a corrosive and possibly explosive atmosphere. • Remove the caps from two Reagent Vials. ← green label → • Add exactly 0.
• Select the Oxygen Demand, (COD) HR method following one of the procedures described in the “Method Selection” section (see page 15). • Place the blank vial into the holder and push it completely down. • Press the Zero key. The instrument will perform a zero sequence and after a few seconds the display will show “-0.0-”. Now the meter is zeroed and ready for measurement. • Remove the blank vial. • Place the sample vial into the holder and push it completely down. • Press Read.
PHOSPHORUS, REACTIVE SPECIFICATIONS Range Resolution Accuracy Light Source Method 0.00 to 1.60 mg/L P 0.01 mg/L ±0.05 mg/L or ±5 % of reading @ 25 °C, whichever is greater Tungsten lamp with narrow band interference filter @ 610 nm Adaptation of the EPA method 365.2 and Standard Methods for the Examination of Water and Wastewater, 20th edition, 4500-P E, ascorbic acid method. The reaction between orthophosphate and the reagent causes a blue tint in the sample.
• Remove the cap and add the content of one packet of HI 93758-0 Phosphorus Reagent. • Replace the cap tightly and shake gently to mix for 2 minutes until most of the powder is dissolved. This is the reacted sample. • Place the vial into the holder and push it completely down. • Press Timer and the display will show the countdown prior to the measurement and the “Reaction Time” message. Alternatively, wait for 3 minutes and press Read. • The instrument will perform the reading.
PHOSPHORUS, ACID HYDROLYZABLE SPECIFICATIONS Range Resolution Accuracy Light Source Method 0.00 to 1.60 mg/L P 0.01 mg/L ±0.05 mg/L or ±5 % of reading @ 25 °C, whichever is greater Tungsten lamp with narrow band interference filter @ 610 nm Adaptation of the EPA method 365.2 and Standard Methods for the Examination of Water and Wastewater, 20th edition, 4500-P E, ascorbic acid method. A mild acid digestion converts condensed inorganic forms of phosphates to orthophosphate.
• Remove the cap from a Reagent Vial. • Add exactly 5.0 mL of sample to the vial, while keeping the vial at a 45-degree angle. • Replace the cap and mix by inverting the vial a couple of times. • Insert the vial into the reactor and heat it for 30 minutes at 150 °C. • At the end of the digestion place the vials carefully in the test tube rack and allow to cool to room temperature. Warning: The vials are still hot, be careful when handling them.
• Press the Zero key. The instrument will perform a zero sequence and after a few seconds the display will show “-0.0-”. Now the meter is zeroed and ready for measurement. • Remove the vial. • Remove the cap and add the content of one packet of HI 93758-0 Phosphorus Reagent. • Replace the cap tightly and shake gently to mix for 2 minutes until most of the powder is dissolved. This is the sample. • Place the vial into the holder and push it completely down.
INTERFERENCES Interference may be caused by: Arsenate at any level Silica above 50 mg/L Sulfide above 9 mg/L. To eliminate sulfide: add Bromine Water drop-wise until a pale yellow color develops; remove Bromine Water excess by adding Phenol solution drop-wise. Turbidity and suspended matter in large amounts may cause interference because the strongly acidic reaction conditions may dissolve suspended matter or cause desorption of phosphates from particles.
PHOSPHORUS, TOTAL LOW RANGE SPECIFICATIONS Range Resolution Accuracy Light Source Method 0.00 to 1.15 mg/L P 0.01 mg/L ±0.05 mg/L or ±6 % of reading @ 25 °C, whichever is greater Tungsten lamp with narrow band interference filter @ 610 nm Adaptation of the EPA method 365.2 and Standard Methods for the Examination of Water and Wastewater, 20th edition, 4500-P E, ascorbic acid method. A persulfate digestion converts organic and condensed inorganic forms of phosphates to orthophosphate.
• Preheat the Hanna Reactor HI 839800 to 150 °C (302 °F). For correct use of the reactor follow Reactor Instruction Manual. Use of the optional HI 740217 safety shield is strongly recommended. DO NOT USE AN OVEN OR MICROWAVE samples may leak and generate a corrosive and possibly explosive atmosphere. • Remove the cap from a Reagent Vial. • Add exactly 5.0 mL of sample to the vial, while keeping the vial at a 45-degree angle. • Add the content of one packet of Potassium Persulfate for Phosphorus analysis.
• Press the Zero key. The instrument will perform a zero sequence and after a few seconds the display will show “-0.0-”. Now the meter is zeroed and ready for measurement. • Remove the vial. • Remove the cap and add the content of one packet of HI 93758-0 Phosphorus Reagent. • Replace the cap tightly and shake gently to mix for about 2 minutes until all the powder is completely dissolved. This is the sample. • Place the vial into the holder and push it completely down.
Note: For accurate measurements 1) wash glassware only with phosphate-free detergents 2) clean all glassware with 1 : 1 hydrochloric acid solution and rinse with deionized water. INTERFERENCES Interference may be caused by: Arsenate at any level Silica above 50 mg/L Sulfide above 90 mg/L. Turbidity and suspended matter in large amounts may cause interference because the strongly acidic reaction conditions may dissolve suspended matter or cause desorption of phosphates from particles.
PHOSPHORUS, REACTIVE HIGH RANGE SPECIFICATIONS Range Resolution Accuracy Light Source Method 0.0 to 32.6 mg/L P 0.1 mg/L ±0.5 mg/L or ±5 % of reading @ 25 °C, whichever is greater Tungsten lamp with narrow band interference filter @ 420 nm Adaptation of the Standard Methods for the Examination of Water and Wastewater, 20th edition, 4500-P C, vanadomolybdophosphoric acid method. The reaction between orthophosphate and the reagents causes a yellow tint in the sample. REQUIRED REAGENTS Code Description Q.
• Replace the cap and mix by inverting each vial a couple of times. Sample Sample D.I. Water Sample Blank Blank • Place the blank vial into the holder and push it completely down. • Press Timer and the display will show the countdown prior to the measurement and the “Reaction Time” message. Alternatively, wait for 7 minutes and press Zero. • The instrument will perform a zero sequence and after a few seconds the display will show “-0.0-”. Now the meter is zeroed and ready for measurement.
Note: For accurate measurements 1) wash glassware only with phosphate-free detergents 2) clean all glassware with 1 : 1 hydrochloric acid solution and rinse with deionized water. INTERFERENCES Interference may be caused by: Bismuth Fluoride pH: the sample should have a neutral pH Sulfide: to eliminate sulfide add Bromine Water drop-wise until a pale yellow color develops; remove Bromine Water excess by adding Phenol solution drop-wise. Temperature: the method is temperature sensitive.
PHOSPHORUS, TOTAL HIGH RANGE SPECIFICATIONS Range Resolution Accuracy Light Source Method 0.0 to 32.6 mg/L (P) 0.1 mg/L ±0.5 mg/L or ±5 % of reading @ 25 °C, whichever is greater Tungsten lamp with narrow band interference filter @ 420 nm Adaptation of the Standard Methods for the Examination of Water and Wastewater, 20th edition, 4500-P C, vanadomolybdophosphoric acid method. A persulfate digestion converts organic and condensed inorganic forms of phosphates to orthophosphate.
Reagent Blank Correction: This method requires a reagent blank correction. A single blank vial may be used more than once; the blank vial is stable up to one day (room temperature). For most accurate measurement, run a blank for each set of measurements and always use the same lot of reagents for blank and samples. • Preheat the Hanna Reactor HI 839800 to 150 °C (302 °F). For correct use of the reactor follow Reactor Instruction Manual. The optional HI 740217 safety shield is strongly recommended.
• Select the Phosphorus, Total High Range method following one of the procedures described in the “Method Selection” section (see page 15). • Remove the cap from the vials and add exactly 2.0 mL of Sodium Hydroxide (NaOH) Solution 1,54 N to each vial, while keeping the vials at a 45-degree angle. Replace the cap tightly and mix by inverting the vials a couple of times. Sample Blank Sample Blank • Remove the cap from the vials and add exactly 0.
• Remove the blank vial. • Place the sample vial into the holder and push it completely down. • Press Read. The instrument will perform the reading. The instrument displays concentration in mg/L of phosphorus (P). The method detects free (orthophosphate), condensed inorganic forms (meta-, pyro- and other polyphosphates) and organic forms of phosphates present in the sample. • Press s or t to convert the result in mg/L of phosphate (PO43-) or mg/L of P2O5.
ERRORS AND WARNINGS The instrument shows clear messages when erroneous conditions appear and when measured values are outside the expected range. These messages are described bellow. No Light: The light source is not functioning properly. Light Leak: There is too much light when dark current is measured. Inverted vials: The sample and the zero vials are inverted to each other. Light Low: The instrument cannot adjust the light level. Please check that the sample does not contain any debris.
DATA MANAGEMENT The analyzed data can be managed using Hanna’s product HI92000, Windows ® Compatible Software. STANDARD METHODS Description Range Method Ammonia LR Ammonia HR Chlorine, Free Chlorine, Total Nitrate Nitrogen, Total LR Nitrogen, Total HR COD LR COD MR COD HR Phosphorus, Reactive Phosphorus, Acid Hydrolyzable Phosphorus, Total LR Phosphorus, Reactive HR Phosphorus, Total HR 0.00 to 3.00 mg / L 0 to 100 mg / L 0.00 to 5.00 mg / L 0.00 to 5.00 mg / L 0.0 to 30.0 mg / L 0.0 to 25.
ACCESSORIES REAGENT SETS HI 93701-01 HI 93701-03 HI 93711-01 HI 93711-03 HI 93754A-25 HI 93754B-25 HI 93754C-25 HI 93758A-50 HI 93758B-50 HI 93758C-50 HI 93763A-50 HI 93763B-50 HI 93764A-25 HI 93764B-25 HI 93766-50 HI 93767A-50 HI 93767B-50 100 free chlorine tests 300 free chlorine tests 100 total chlorine tests 300 total chlorine tests 25 COD LR tests 25 COD MR tests 25 COD HR tests 50 reactive phosphorus tests 50 acid hydrolyzable phosphorus tests 50 total phosphorus LR tests 50 reactive phosphorus HR te
WARRANTY All Hanna Instruments meters are warranted for two years against defects in workmanship and materials when used for its intended purpose and maintained according to the instructions. This warranty is limited to repair or replacement free of charge. Damages due to accident, misuse, tampering or lack of prescribed maintenance are not covered. If service is required, contact your dealer. If under warranty, report the model number, date of purchase, serial number and the nature of the failure.
Recommendations for Users Before using these products, make sure that they are entirely suitable for your specific application and for the environment in which they are used. Operation of these instruments may cause unacceptable interferences to other electronic equipments, this requiring the operator to take all necessary steps to correct interferences. Any variation introduced by the user to the supplied equipment may degrade the instruments' EMC performance.
Hanna Instruments Inc. Highland Industrial Park 584 Park East Drive Woonsocket, RI 02895 USA Technical Support for Customers Tel. (800) 426 6287 Fax (401) 765 7575 E-mail tech@hannainst.com www.hannainst.
