Instruction Manual HI 84185 ISE Ammonia Nitrogen Meter w w w. h a n n a i n s t .
Dear Customer, Thank you for choosing a Hanna product. This manual will provide you with the necessary information for the correct use of the instrument. Please read it carefully before using the meter. If you need additional technical information, do not hesitate to e-mail us at tech@hannainst.com. directives. This instrument is in compliance with TABLE OF CONTENTS PRELIMINARY EXAMINATION.................................................................................................
PRELIMINARY EXAMINATION Please examine this product carefully. Make sure that the instrument is not damaged. If any damage occurred during shipment, please notify your Dealer. Each HI 84185 instrument is supplied complete with: • Reagents set for 20 tests • One 1000 µL automatic pipette • Six plastic tips for the 1000 µL automatic pipette • Two 50 mL beakers • HI 61101 Ammonia electrode • HI 7662-T Temperature probe • Stir bar (2 pcs.
GENERAL DESCRIPTION The HI 84185 is a low cost, easy to use, ammonia nitrogen (N-NH3) ISE instrument that performs automatic analysis with all the necessary calculations assuring to the user a simple and effective interface. The instrument comes with a powerful and effective built-in algorithm to analyse the shape of the ISE electrode response and to determine the reaction completion.
SPECIFICATIONS Range Resolution Accuracy Sample volume Temperature Compensation Electrode Temperature Probe LOG feature Stirring speed Environment Power supply Dimensions Weight REQUIRED REAGENTS Code HI 84185-0 HI 84185-1 HI 84185-2 0 to 50 mg/L (ppm) N-NH3 1 mg/L (ppm) N-NH3 ±5% of reading 50 mL Automatic from 0 to 80 °C HI 61101 Ammonia combined ISE HI 7662-T (included) 50 samples 500 rpm 0 to 50 °C (32 to 122 °F); max 95% RH non-condensing 220V/50Hz; 10VA 208 x 214 x 163 mm (8.2 x 8.4 x 6.
PRINCIPLE OF OPERATION The determination of ammonia nitrogen concentration is made according to double standard addition method. This technique enables an unknown concentration and the electrode’s slope factor to be determined simultaneously. The Automatic Temperature compensation is available using HI 7662-T temperature probe. By setting the electrode isopotential point and enabling temperature compensation option in Setup menu, the obtained concentration is temperature compensated.
FUNCTIONAL DESCRIPTION INSTRUMENT DESCRIPTION FRONT PANEL 1) 2) 3) 4) Graphic LCD Functional keys Keypad Electrode holder REAR PANEL 5) 6) 7) 8) 9) Ammonia electrode Temperature probe Electrode holder Reference socket Temperature probe socket 10) 11) 12) 13) 7 BNC electrode connector Power switch Fuse Power cable connector
KEYPAD DESCRIPTION 1) ESC - to leave the current screen and to return either to the previous screen or to the main screen. In the setup parameter modifying mode by pressing ESC the value is not modified. 2) - to modify a parameter value, to scroll the information from the instrument’s setup 3) HELP - to access/leave the instrument’s contextual help 4) - to modify a parameter value, to scroll the information from the instrument’s setup 5) Functional Keys START UP • Place the instrument on a flat table.
GUIDE TO DISPLAY CODES During the instrument’s operation a series of informations are displayed on the LCD. 1) current time information area 2) instrument status area 3) functional keys area 4) stability criteria 5) stirrer and reading status area Icons from the stirrer and reading status area When the input reading is unstable. When the stirrer is on. Temperature warning (only if the “Temperature compensation” is disabled).
If the difference between the temperature of the sample confirmed at the first step and the subsequent readings is greater than 3°C and the temperature compensation feature is disabled in Setup, the temperature warning icon will blink and the message “WARNING: Large temperature change!” will be displayed. Pause screen Press Resume to continue the measurement process. Press Stir to start/stop the stirrer.
Result screen The ion concentration is displayed in mg/L (ppm) N-NH3. If the concentration is out of range, the instrument displays the upper range limit blinking. Press LOG to save the measured concentration. A message indicating the free log space will be displayed for a few seconds. If the free log space is under 10% this message will be permanently displayed. Press Start to begin a new measurement cycle. Wrong slope screen This screen appears if the computed slope is not in the acceptable range.
Isopotential point Options: 1.0 to 50.0 mg/L (ppm). Press Modify to access the isopotential point concentration modify screen. Press / to increase/decrease the isopotential point concentration. Press Accept to confirm the value or ESC to return to the Setup menu without saving. If the temperature compensation feature is disabled the meter will display “None” for the isopotential point, and the isopotential point modify screen cannot be accessed. Concentration unit Options: mg/L, ppm.
Date/Time This option is used to set the instrument’s date and time. Press Modify to access the date/time modify screen. Date/Time modify screen Press / to select the value to be modified (year, month, day, hour, minute or second) and then press / in order to increase/decrease the value. Press Accept to confirm the new values or ESC to return to Setup menu without saving. Time format Options: AM/PM or 24 hours. Press the functional key in order to change the time format.
Beep status Options: Enable, Disable This option enables/disables the beep feature. Press the functional key in order to select the new option. When enabled, beep sounds as a short beep every time a key is pressed or when the reading must be confirmed. In auto confirm mode the instrument will beep until the standard addition is detected. A long beep alerts that the pressed key is not active. Reading confirm Option: Enable, Disable Enable this feature to manually confirm a stable reading.
Recall screens Displays the list of all the memorized results. This list includes the record’s number, the concentration value, the concentration unit and the date. Press / to toggle between the stored records. Press More to view a complete set of data corresponding to the selected record. To return to the previous screen press ESC. Press / to toggle between complete record information screens. Press Delete to erase the currently selected record from the instrument’s memory.
TIPS FOR AN ACCURATE MEASUREMENT The instructions listed bellow should be carefully followed during analysis to ensure best accuracy. • Rinse the electrode with distilled or deionized water between samples, and blot dry taking care not to touch de membrane. • Select the adequate stability criteria. • Analyse the wine sample at room temperature.
• Use the 1000 µL automatic pipette to add exactly 1 mL of HI 84185-1 Standard and immediatly close the stopper. • Wait until the reading is stable, CFM key displayed, if the “Reading confirm” option is selected, and then press CFM. If the “Reading confirm” option is disabled the instrument automatically memorizes the reading when this is stable and waits to add standard. Until the standard addition is detected the instrument will beep if beeper is enabled in Setup.
FUSE REPLACEMENT To change the fuse follow next steps: • Disconnect the power cord from the rear panel of the instrument. • Pull out the fuse holder located near the power cord connector. • Replace the fuse with a similar one. • Push the fuse holder with the fuse in the appropriate place.
INNER ELECTRODE CHECK • Before assembling the electrode for the first time or reactivating it after storage, the inner electrode assembly should be hydrated then tested as a pH electrode. • Take two beakers and pour 50 mL de-ionized water in each of them. • Add one powder packet of HI 4000-47-4 pH 4 in one of the beakers. • Add one powder packet of HI 4000-47-7 pH 7 in the other beaker. • Remove the protective shipping cap from the glass inner electrode.
• Slide electrode cap and spring down cable and screw top cap onto outer body until fully engaged. Afterwards, unscrew top cap two times. Do not invert electrode. Keep the membrane side down. • Gently pull spring loaded cable back and slowly release to allow filling solution to migrate between membrane and glass electrode inner body. Screw top cap onto outer body until fully engaged. • Install assembled electrode in gas sensor test vessel or in electrode holder and connect cable connector to the meter.
QUICK CHECK OF THE ELECTRODE SLOPE • Connect the electrode and the reference electrode to the instrument. • Press and hold down ESC, and HELP keys simultaneously and power on the meter. Wait until the instrument enters in the reading check mode. • Fill a beaker with 50 mL of deionized water and put a stir bar inside. Place the beaker in its appropriate place on the instrument top. • Put the electrode holder and insert the electrode and temperature probe in the solution. Add 2 mL of HI 84185-0 ISA buffer.
ACCESSORIES REAGENT SETS HI 4001-40 HI 4001-45 HI 4000-47 HI 84185-20 HI 84185-0 HI 84185-1 HI 85185-2 Ammonia filling solution Ammonia conditioning and storage solution Buffer replacement kit Reagent set ISA for Ammonia electrode (500 mL) Standard 1 for Ammonia electrode (500 mL) Standard 2 for Ammonia electrode (500 mL) OTHER ACCESSORIES HI 61001-51 Membrane (20 pcs.) HI 61101 Ammonia electrode HI 731316 Stir bar (5 pcs.
WARRANTY HI 84185 is warranted for two years against defects in workmanship and materials when used for its intended purpose and maintained according to the instructions. This warranty is limited to repair or replacement free of charge. Damage due to accident, misuse, tampering or lack of prescribed maintenance is not covered. If service is required, contact your dealer. If under warranty, report the model number, date of purchase, serial number and the nature of the failure.
SALES AND TECHNICAL SERVICE CONTACTS Australia: Tel. (03) 9769.0666 • Fax (03) 9769.0699 China: Tel. (10) 88570068 • Fax (10) 88570060 Egypt: Tel. & Fax (02) 2758.683 Germany: Tel. (07851) 9129-0 • Fax (07851) 9129-99 Greece: Tel. (210) 823.5192 • Fax (210) 884.0210 Indonesia: Tel. (210) 4584.2941 • Fax (210) 4584.2942 Japan: Tel. (03) 3258.9565 • Fax (03) 3258.9567 Korea: Tel. (02) 2278.5147 • Fax (02) 2264.1729 Malaysia: Tel. (603) 5638.9940 • Fax (603) 5638.9829 Singapore: Tel. 6296.
