Instruction Manual HI 84432 TITRATABLE ACIDITY MINITITRATOR & pH METER for Fruit Juices w w w. h a n n a i n s t .
Dear Customer, Thank you for choosing a Hanna product. This manual will provide you with the necessary information for the correct use of the instrument. Please read it carefully before using the meter. If you need additional technical information, do not hesitate to e-mail us at tech@hannainst.com.
TABLE OF CONTENTS PRELIMINARY EXAMINATION................................................................................................. 4 GENERAL DESCRIPTION ........................................................................................................ 4 SPECIFICATIONS ................................................................................................................... 6 PRINCIPLE OF OPERATION ........................................................................................
PRELIMINARY EXAMINATION Please examine this product carefully. Make sure that the instrument is not damaged. If any damage occurred during shipment, please notify your Dealer. Each HI 84432 minititrator is supplied complete with: • HI 1131B pH electrode • HI 7662-M Temperature probe • HI 84432-50 Titrant solution (100 mL) • HI 84432-55 Pump calibration solution (100 mL) • HI 70004 pH 4.01 buffer solution (2 x 20 mL) • HI 70007 pH 7.01 buffer solution (2 x 20 mL) • HI 700082 pH 8.
The Titratable Acidity determination is instantaneously displayed in selected measurement units on the large dot matrix display. The instrument is ready for the next analysis immediately.
SPECIFICATIONS Titrator Range Titratable acidity (low range): 15 mL sample g/100 mL as citric acid: 0.20 - 1.20% CA g/100 mL as tartaric acid: 0.23 - 1.41% TA g/100 mL as malic acid: 0.21 - 1.26% MA Titratable acidity (high range): 2 mL sample g/100 mL as citric acid: 0.80 - 8.00% CA g/100 mL as tartaric acid: 0.94 - 9.30% TA g/100 mL as malic acid: 0.84 - 8.30% MA Resolution 0.01% Accuracy 5% of reading or ± 0.02 Titration method Acid-base titration Principle End point titration: 8.20 pH Pump debit 0.
REQUIRED REAGENTS Code HI 84432 - 50 HI 84432 - 55 Description Titrant Pump Calibration Solution Quantity/Test 1 mL 2 mL PRINCIPLE OF OPERATION Fruit juice acidity is determined by neutralization of all available hydrogen ions present in the sample, with a strong base solution: H++HO- H2O In an ideal solution, the end point of an acid titration corresponds stoichiometrically to the complete neutralization of the acids present.
FUNCTIONAL AND PHYSICAL DESCRIPTION OVERHEAD VIEW 1. 2. 3. 4. Titrant bottle Graphic LCD Keypad Dedicated guide tube for Temperature Probe 5. Electrode holder 6. Dedicated guide tube for titrant tip 7. Peristaltic pump tube FRONT VIEW REAR VIEW 8. 9. 10. 11. 12. 13. 14. 15.
KEYPAD FUNCTION AND INDICATORS ESC / HELP MENU STIR Note: - used to leave the current screen and to return either to the previous screen or to the main screen. When pressed while modifying a parameter within the SETUP menu, exits parameter without changing it.
GUIDE TO INDICATORS During the instrument’s operation information is displayed on the LCD. Displayed icons: Pump running. Unstable reading. Stirrer on. Parameter can be changed. 1. Current time and instrument mode information (pH meter or Titrator) 2. Main reading information 3. Instrument status information 4. Functional key area 5. Indicates that the displayed value can be changed using ARROW keys 6. Temperature value (Manual or Automatic) 7. pH temperature compensation mode (Manual or Automatic) 8.
TITRATOR STARTUP This is a general outline of the steps required to make a titration. The following topics are expanded upon each section that follows. • Place the instrument on a flat table. Do not place the instrument in direct sun light. • Connect the power adapter to the instrument. • Turn the instrument ON using the power switch from the rear panel of the instrument. • Set up the instrument. See the “Setup Configuration Menu” section for details. Pay attention to the Acidity Unit.
SETUP CONFIGURATION MENU The titrator’s setup configuration menu may be accessed from the pH or titration screens by pressing the MENU key, then Setup. A list of setup parameters will be displayed with currently configured setting. While in the setup menu it is possible to modify the instrument’s operation parameters. The ARROW keys permit the user to scroll the setup parameters. Press HELP to view the contextual help. Press ESC to return to the main screen. Concentration unit Options: %CA; %TA; %MA.
Temperature unit Options: °C, °F. Press the function key in order to change the temperature unit. Backlight Options: 0 to 8. Press Modify to access the backlight level. Use the ARROW keys or / in order to increase/decrease the displayed constrast. Press Accept to confirm or ESC to return to the setup menu. Contrast Option: 0 to 20. This option is used to set the display’s contrast. Press Modify to change the display’s contrast. Use the ARROW keys or / in order to increase/decrease the value.
Date / Time This option is used to set the instrument’s date and time. Press Modify to change the date/time. Press / to highlight the value to be modified (year, month, day, hour, minute or second). Use the ARROW keys to change the value. Press Accept to confirm the new value or ESC to return to the setup. Time format Option: AM/PM or 24 hours. Press the functional key to select the new value. Date format Press Modify to change the Date Format. Use the ARROW keys to select the desired format.
Language Press the corresponding function key to change the language. If the new selected language cannot be loaded, the previously selected language will be reloaded. If no language can be loaded at startup the instrument will work in the “safe mode”. In “safe mode” all the messages are displayed in English and help information are not available. Beep On Press the function key to select the new option. When enabled, a short beep is heard every time a key is pressed or when the calibration can be confirmed.
ELECTRODE PREPARATION PREPARATION PROCEDURE Remove the electrode protective cap. DO NOT BE ALARMED IF ANY SALT DEPOSITS ARE PRESENT. This is normal with electrodes and they will disappear when rinsed with distilled water. During transport, tiny bubbles of air may have formed inside the glass bulb. The electrode cannot function properly under these conditions. These bubbles can be removed by "shaking down" the electrode as you would do with a glass thermometer.
ELECTRODE CALIBRATION PROCEDURE It is recommended to calibrate the instrument frequently, especially if high accuracy is required. The pH electrode should be recalibrated: a) Whenever the pH electrode is replaced b) At least once a week, but daily is advised c) After electrode is cleaned d) When high accuracy is required e) If the pH calibration expired warning is displayed during measurement.
• The (unstable measurement) symbol will be shown on the display until the reading becomes stable. • When the reading is stable and close to the selected buffer, the (unstable measurement) symbol will disappear and the Confirm key will become active. • Press Confirm to confirm the calibration. • Press ESC to exit calibration. Point 2 calibration • The calibrated value will be shown on the display and the second expected buffer value will be displayed.
Point 3 calibration • Remove the electrode holder with electrode from the top of the beaker. • Place the third buffer solution in a beaker and place in beaker holder. Rinse the probes in a beaker with third buffer rinsing solution. • Place the electrode holder (with electrode) in the beaker with third buffer and secure holder by locking. • The (unstable measurement) symbol will be shown on the display until the reading becomes stable.
• If the computed slope isn’t within the preset limits the meter will display the will be message “Wrong Slope”. If the slope is too high the symbol will be displayed. displayed. If the slope is too low the symbol • If the “Wrong Old Slope” error message is displayed, an inconsistency exists between the current and the previous (old) calibration. Clear the calibration parameters by pressing Clear and proceed with calibration from the current calibration point.
Notes: • To clear a previous calibration and to return to the default value, press Clear at any time after entering calibration mode. The “Calibration cleared” message will be shown for a few seconds on the display. If Clear is invoked during the first calibration point the instrument returns to the measurement mode. • The Clear key is displayed only if a previous calibration exists.
pH BUFFER TEMPERATURE DEPENDENCE The temperature has an effect on pH. The calibration buffer solutions are affected by temperature changes to a lesser degree than normal solutions. During calibration the instrument will automatically calibrate to the pH value corresponding to the measured or set temperature. During calibration the instrument will display the pH buffer value at 25 °C. TEMP pH BUFFERS °C °F 4. 01 7. 01 8. 20 0 32 4.01 7.13 8.38 5 41 4.00 7.10 8.34 10 50 4.00 7.07 8.
PUMP TUBE INSTALLATION To mount the new peristaltic pump tube follow next steps: • Position one tubing ferrule ring on its location. • Stretch the tube over the peristaltic pump rollers. • Fix the second tube ferrulering ring on its location. • Attach the tube to the reagent bottle. Note: Purge the peristaltic pump until drops of reagent appears on the dosing tip by pressing the PURGE key from the titrator main screen.
PURGE Purging should be performed: • if you notice there is no titrant in the tip; • whenever the tube of the peristaltic pump is replaced; • whenever the titrant is changed or a new bottle is used; • before starting a pump calibration; • before starting a lot of titrations. In order to start purging, press the Purge key from the titrator main screen. The purging stops automatically after 5 minutes. To access the Purge key follow the next steps.
• Press Purge to begin a purge cycle. The purging stops automatically after 5 minutes. To stop purging at any time and return to the main screen press ESC or Stop. During a purge, a countdown timer displays remaining time in 5 minute purge. Press Pause to interrupt the purge process.
PUMP CALIBRATION PROCEDURE Please set up the instrument before performing the pump calibration. The calibration of the pump must be performed each time the pump tube, the reagent bottle or the pH electrode is changed. It is recommended to daily perform the pump calibration before you start titrations. Verify: The electrode has been calibrated in 8.20 pH buffer. • Sample preparation: Precisely measure 2 mL of HI 84432-55 Pump Calibration Solution and add it to a clean beaker.
• Press Pump. The next screen will be displayed. • Press Start. • After the pump calibration is started, on the upper right side of the display two animations will be shown in order to indicate that the pump and the stirrer are working. On the lower right side of the display is shown the amount of time that has passed since the beginning of the calibration.
Notes: • The calibration of the pump is dependent on concentration unit selected during SETUP. • If an erroneous situation is encountered during the calibration, an error message is displayed and the calibration can be restarted by pressing Restart. • Use a new sample of standard and clean electrode and temperature probe before pressing Restart.
TITRATION PROCEDURE Verify: The instrument has been calibrated (pH and pump) before performing a lot of titrations. An electrode calibration in at least one point (8.20 pH buffer) is recommended. In order to increase the result accuracy, the measuring range was divided in two ranges: Low range (LR): 0.20 to 1.20% CA High range (HR): 0.80 to 8.
• Press Start to begin the titration process. • After the titration is started two animations will be shown on the upper right side of the display to indicate that the pump and the stirrer are running. On the lower right side of the display is shown the period of time since the titration has been started. • After the titration is complete, the concentration value is displayed in the selected unit. • Press Unit to change the display unit.
• If the concentration is out of limits an exceeded range limit message will be displayed blinking and the message “Out of range” will be shown. Another titration can be initiated by pressing Restart. Prepare a fresh sample and then press Restart. Note: If the end-point is not reached or it is not recognized or the input reading is out of range, an error message will be displayed. Also the sign “<” underrange or “>” overrange is shown.
Use the ARROW keys to scroll the stored records list. If the saved concentration was out of range the “!” and “<” / “>” symbol (underrange/overrange) is displayed in front of the reading. Press Delete to enter record deleting mode. Press Delete All to enter all records deleting mode. Press More to view more information. Press Unit to convert the result to other unit. Use the ARROW keys when is displayed to scroll between the log records. Press ESC to return to the previous screen.
Press Confirm to delete all the records or ESC to return to the previous screen. If the titrator log is empty the message “No Records!” will be displayed. TITRATOR GLP INFORMATION Press MENU while in Titration mode and then GLP. From this screen it is possible to select between viewing the electrode GLP or the pump GLP. Press GLP elec. to view the electrode’s last calibration parameters and date. Press GLP pump to view the pump’s last calibration time and date. If GLP elec.
If GLP pump is pressed, one of the next screens is displayed. The pump GLP displays the Time and Date of the last pump calibration. If a calibration hasn’t been performed the message “Not Calibrated” will be displayed. pH MEASUREMENT The HI 84432 may be used as a pH meter for direct measurements. Verify that the instrument has been calibrated before taking pH measurements. Set the instrument to pH meter. At power up the instrument enters pH meter mode.
Press HELP to view the contextual help, every time you need supplementary information. The help is customized for every situation that can appear during instrument usage. Press STIR to start/stop the stirrer. Press Titrator to enter titrator mode. Press CAL to access the calibration menu. Press LOG to memorize the current reading. A message indicating the free log space will be displayed for a few seconds.
of measurements. If Manual Temperature Compensation (MTC) is desired, the temperature probe must be disconnected from the instrument. The default temperature of 25 °C (77 °F) or the last temperature reading will be displayed preceded by the symbol and the “MTC” message. The manually set temperature can now be adjusted with the ARROW keys (from -20.0 to 120.0 °C). VIEW/DELETE RECORDED pH DATA To view or delete previously logged pH records press MENU key while in pH meter screen.
Deleting a record will renumber the list of records. If Delete All was pressed the instrument will ask for confirmation. Press Confirm to delete all records or ESC to return to the previous screen without deleting. If the pH log is empty the message “No Records!” will be displayed. pH METER GLP INFORMATION The pH meter GLP screens references the last pH calibration data. GLP is an acronym for Good Laboratory Practice. To view this information, press MENU key then GLP.
TEMPERATURE CALIBRATION PROCEDURE (for technical personnel only) All the instruments are factory calibrated for temperature. HANNA’s temperature probes are interchangeable and no temperature calibration is needed when they are replaced. If the temperature measurements are inaccurate, temperature recalibration should be performed. For an accurate recalibration, contact your dealer or the nearest HANNA Customer Service Center, or follow the instructions below.
• Immerse the temperature probe in the second vessel as near as possible to the reference thermometer. Allow a few seconds for the probe to stabilize. • Use the ARROW keys to set the calibration point value to that of the hot water, measured by the reference thermometer. • The (unstable measurement) symbol will be shown on the display until the reading becomes stable.
TROUBLESHOOTING GUIDE SYMPTO MS Slow resp onse/excessive d rift. Rea d ing flu ctu a tes u p a nd d own (noise). PRO BLEM D irty p H electrod e. Clog g ed /d irty ju nction. L ow electrolyte level (refilla b le p H electrod es only). Ca b le connection. SO LU TI O N Soa k the electrod e tip in HI 7061 clea ning solu tion for 30 minu tes. Refill with fill solu tion. Soa k the electrod e tip in HI 7061 clea ning solu tion for 30 minu tes. Refill with fill solu tion.
ELECTRODE CONDITIONING AND MAINTENANCE STORAGE PROCEDURE To assure a quick response time, the glass bulb should be kept moist and not allowed to dry out. Replace the solution in the protective cap with a few drops of HI 70300 or HI 80300 Storage Solution. Follow the Preparation Procedure section before taking measurements. Note: NEVER STORE THE pH ELECTRODE IN DISTILLED OR DEIONIZED WATER. PERIODIC MAINTENANCE Inspect the electrodes and the cables.
ACCESSORIES REAGENTS HI 84432-50 HI 84432-55 HI 84432-70 Titrant solution (100mL) Pump calibration solution (100mL) Reagents kit (about 150 titrations) pH CALIBRATION SOLUTIONS HI 7004M Buffer solution pH 4.01 HI 7007M Buffer solution pH 7.01 HI 70082M Buffer solution pH 8.20 HI 7004M-6 Buffer solution pH 4.01 HI 7007M-6 Buffer solution pH 7.01 HI 70082M-6 Buffer solution pH 8.
WARRANTY HI 84432 is warranted for two years against defects in workmanship and materials when used for its intended purpose and maintained according to the instructions. This warranty is limited to repair or replacement free of charge. Damage due to accident, misuse, tampering or lack of prescribed maintenance is not covered. If service is required, contact your dealer. If under warranty, report the model number, date of purchase, serial number and the nature of the failure.
Hanna Instruments Inc. Highland Industrial Park 584 Park East Drive Woonsocket, RI 02895 USA Local Sales and Customer Service office Hanna Instruments United States Inc. Highland Industrial Park 584 Park East Drive Woonsocket, RI 02895 USA Tel. (800) 426 6287 Fax (401) 765 7575 www.hannainst.com/usa Technical Support for customers Telephone (800) 426 6287 Fax (401) 765 7575 E-mail tech@hannainst.
