Instruction Manual HI 4421 DO/BOD/OUR/SOUR/Temperature Bench Meter w w w. h a n n a i n s t .
Dear Customer, Thank you for choosing a Hanna Instruments product. This manual will provide you with the necessary information for correct use of the instrument. Please read this instruction manual carefully before using the instrument. If you need additional technical information, do not hesitate to e-mail us at tech@hannainst.com or see the back side of this manual for our worldwide sales and technical service contacts. These instruments are in compliance with directives.
TABLE OF CONTENTS WARRANTY ...................................................................................................................................... 2 PRELIMINARY EXAMINATION .............................................................................................................. 4 GENERAL DESCRIPTION ...................................................................................................................... 4 FUNCTIONAL DESCRIPTION ...............................................
PRELIMINARY EXAMINATION Remove the instrument from the packing material and examine it carefully to make sure that no damage has occurred during shipping. If there is any damage, notify your dealer or the nearest Hanna Service Center.
FUNCTIONAL DESCRIPTION HI 4421 DESCRIPTION FRONT PANEL REAR PANEL 1) 2) 3) 4) 5) 6) 7) Liquid Crystal Display (LCD) Main Keyboard USB connector ON/OFF switch Power adapter socket RS232 serial communication connector DO probe input 5
KEYBOARD DESCRIPTION FUNCTION KEYS To enter / exit calibration mode. To select the desired measurement mode: DO, BOD, OUR, SOUR. To enter Setup (System Setup, DO Setup, BOD Setup , OUR Setup or SOUR Setup) and to access Log Recall function. To obtain general informations about the selected option / operation. VIRTUAL KEYS The upper row keys are assigned to the virtual keys placed on the bottom of the LCD, which allow you to and in Measure perform the displayed function, depending on the current menu (e.g.
SPECIFICATIONS HI 4421 Ra n g e 0.00 to 90.00 p p m 0.0 to 600.0 % sa tu ra tion Resolu tion 0.01 p p m 0.1 % sa tu ra tion Accu ra cy ±1.5% of rea d in g ±1 d ig it Mea su rem en t ra n g e -20.0 to 120.0 °C -4.0 to 248.0 °F 253.1 to 393.1 K D O com p en sa tion ra n g e 0.0 to 50.0 °C 32.0 to 122.0 °F 237.1 to 323.1 K Resolu tion 0.1 °C, °F, K Accu ra cy ±0.
OPERATIONAL GUIDE POWER CONNECTION Plug the 12 Vdc adapter into the power supply socket. Note: These instruments use non volatile memory to retain the meter settings, even when unplugged. PROBE CONNECTION For DO, BOD, OUR or SOUR measurements connect a DO probe to the DIN connector located on the rear panel of the instrument. INSTRUMENT START UP • Turn the instrument on from the power switch located on the rear panel of the instrument. • Please wait until the instrument finishes the initialization process.
DISPLAYING MODES For each measurement mode (DO, BOD, OUR or SOUR) the following display configurations are available: Basic, Good Laboratory Practice (GLP), Graph and Log History. Basic Accessing this option, the measured value and its units are displayed on the LCD, along with the temperature value, temperature compensation mode, pressure value, pressure compensation mode and minimal GLP data. To choose the Basic displaying mode: • Press while in Measure mode.
Graph Accessing this option, the online graph with currently logged values (DO, BOD, OUR or SOUR vs. Seconds) could be displayed. If there is no active log, the previously logged data for the selected parameter will be plotted. Notes: • If no data were logged, the graph displaying mode will not be accessible. • If no automatic log is saved, the offline graph will not be available. To access the offline / online graph: • Press while in Measure mode.
Log History Accessing this option, last logged records will be displayed on the LCD. The log history list also contains the appropriate DO / BOD / OUR / SOUR values, the logged temperature, the temperature source, as well as the records time stamp. To access the Log History displaying mode: • Press while in Measure mode. The “Choose Display Configuration” message will be displayed in the Reminder messages area. • Press . The instrument will display the log history regarding the selected measure mode.
SYSTEM SETUP The System Setup menu allows the user to customize the user interface, consult the meter information, set the external serial communication interface and to restore the manufacturer settings. Accessing System Setup • Press while in Measure mode. . The system setup options will be displayed • Press on the LCD. To access a System Setup option: • Use • Press or to highlight the desired option. to access the selected option.
To set the Beeper: • Use or to select the Beeper option. • Press and use or to highlight the desired beeper associated event you want to modify. and use • Press beeper status option. • Press or to highlight the to confirm your selection and return to the Beeper menu or press to return without changing.
To set the GLP data: • Use or to select the GLP Data option. and use • Press desired option. or to highlight the • Press to edit the desired information. The Text Editor menu will be displayed on the LCD. and to • Enter the desired information by using highlight the desired character. It is also possible to delete the last character by positioning the cursor on the Backspace character ( ) . and pressing • Press to return to the GLP Data menu.
• Use to select the Date & Time option. or • Press and use or desired option you want to modify. to highlight the to confirm your selection. Use • Press and then use and to modify the value with (for Set Date and Time option). For the or other two options press to confirm your selection and select one of the displayed formats with or . to confirm your selection and return to the Date • Press & Time options. • Press to return to the previous mode.
Language This option allows the user to choose the desired language for the user interface. To select the Language: • Use or and use • Press desired language. • Press to select the Language option. or to highlight the to confirm your selection and return to the System Setup menu or press menu without changing.
Meter Information This option provides general information about the instrument serial number (each instrument has an unique identification serial number), the software version and the factory calibration date and time (for DO, pressure and temperature). Note: All the instruments are factory calibrated for DO, pressure and temperature. After one year from last factory calibration, the warning will appear at meter startup to inform the user that a new factory calibration is required.
DO SETUP The DO Setup menu allows the user to set the parameters related to the DO measurement. Accessing DO Setup • Press while in Measure mode and then select the DO measure mode. • Press and then to to access DO Setup menu. To access a DO setup options: • Use or to highlight the desired option. to access the selected option or to • Press exit setup. The following is a detailed description of the DO Setup option screens.
Save Current Profile To save the current profile: or • Use to select the Profile option. and then select Save Current Profile option. The • Press text editor box will be displayed on the LCD. • Enter the desired profile name by using and to to add it highlight the desired character and then press to the text bar. It is also possible to delete the last character by positioning the cursor on the Backspace character ( ) and pressing . • Press to return to the Profile options.
Delete Profile To delete one of the existing profiles: or to select the Profile option. • Use and use • Press Delete Profile option. or to highlight the • Press the screen. . A list with all customised profiles will appear on • Use or to select the desired profile and press . • Press to return to the previous menu. Reading Mode This option allows the user to select between Direct and Direct/AutoHold DO reading modes. To set the reading mode: • Use or and use • Press desired option.
Temperature Units The user can choose from the Celsius, Fahrenheit or Kelvin temperature units. To set the temperature unit: • Use option. or to highlight the Temperature Units and then use or • Press Celsius, Fahrenheit or Kelvin degrees unit. to select • Press to confirm your selection or press cancel operation. to Barometer From the Barometer menu the user can choose the pressure source and units, as well as the pressure.
Pressure To set the pressure: • Use or to highlight the Pressure option. and then use • Press / decrease the value. • Press or to save or press to increase to cancel operation. Pressure Units The user can choose from the mmHg or mbar pressure units. To set the pressure unit: • Use option. or to highlight the Pressure Units and then use • Press between mmHg and mbar units. or to select • Press to confirm your selection or press cancel operation.
Calibration Using DO standards The meter can be calibrated in a single or multi-points (up to two points), using 2 DO standards (0.0%, 100%) or using the custom standards. The following options are available for calibration: Standard Recognition The user can choose between Automatic recognition (from 2 Hanna standards available) or User Standard. or to highlight the Standard Recognition • Use option. or to choose from Automatic or User • Use Standard option.
Set Reminder Period Daily reminder - the user can set the time from the day when the reminder is to appear. Periodic reminder - the user can set the time from the last calibration (days, hours and minutes) after which the reminder appears. To set the reminder period: • Use option. or to highlight the Set Reminder Period and use • Press previous entry to be edited. • Press and use value, then press • Press / to select next / or to set the desired to save the modified value.
Measurement Unit The user can select the desired measurement unit. The available options are: % Sat and mg/L. • Use option. • Press • Use or to highlight the Measurement Unit to confirm your selection. or to select % Sat or mg/L. • Press to confirm your selection or press cancel operation. to Sample ID This option allows the user to give to the measured samples an identification number/name. Two Sample ID options are available: ID Increment and Edit Sample ID.
Edit Sample ID This option allows the user to edit the sample ID (numeric - auto-increment mode, alphanumeric - user editable). To edit the Sample ID: or to select the Sample ID option. • Use and use or to highlight the Edit Sample ID option and then press • Press • Edit numerically / alphanumerically the sample ID. • Press to save the current sample ID or press . to cancel operation.
Logging Data Configuration This option allows the user to select the parameters that accompany a logged value: Date/Time, Calibration Data, Sample ID, Instrument ID, Operator ID, Company Name, Aditional Info 1 and Aditional Info 2. To customise the logging data configuration: or to highlight the Logging Data • Use Configuration option. and then use or to enable • Press the parameter by selecting Yes or to disable it by selecting No for each option. to return to the previous menu.
Alarm This option allows the user to define two alarm limits for the measurements. Alarm State The following options are available: Disabled – the alarm will be disabled. Inside Limits – the alarm will notify the user when the measured value is inside the preset limits. Outside Limits – the alarm will notify the user when the measured value is outside the preset limits. To set the alarm state: • Use to highlight the Alarm State option. or and use • Press desired option.
BOD SETUP The BOD Setup menu allows the user to set the parameters related to the resistivity measurements. Accessing BOD Setup • Press while in Measure mode and then select resistivity range. to access BOD Setup menu. and then • Press to To access a BOD Setup option: or • Use to select the desired option. to confirm your selection. • Press The following is a description of the Resistivity Setup option screens. Profile - see DO Setup section. Temperature Units - see DO Setup section.
Barometer - see DO Setup section. Salinity - see DO Setup section. Sample ID - see DO Setup section. Log - see DO Setup section. Alarm - see DO Setup section.
OUR SETUP The OUR Setup menu allows the user to set the parameters related to the OUR measurement. Accessing OUR Setup • Press while in Measure mode and then select OUR range. • Press and then to to access OUR Setup menu. To access a OUR Setup option: • Use or to highlight the desired option. to access the selected option. • Press The following is a description of the OUR Setup option screens. Profile - see DO Setup section. Temperature Units - see DO Setup section.
Barometer - see DO Setup section. Salinity - see DO Setup section. Sample ID - see DO Setup section. Log - see DO Setup section. Alarm - see DO Setup section.
SOUR SETUP The SOUR Setup menu allows the user to set the parameters related to SOUR measurement and calibration. Accessing SOUR Setup • Press while in Measure mode and then select SOUR range. to access SOUR Setup menu. and then • Press to To access an SOUR Setup option: or • Use to highlight the desired option. • Press to access the selected option. The following is a description of the SOUR Setup options. Profile - see DO Setup section. Temperature - see DO Setup section.
Barometer - see DO Setup section. Salinity - see DO Setup section. Sample ID - see DO Setup section. Log - see DO Setup section. Alarm - see DO Setup section.
DO CALIBRATION It is recommended to calibrate the instrument frequently, especially if high accuracy is required. The DO range should be recalibrated: • Whenever the DO probe is replaced. • At least once a week. • Before BOD, OUR, SOUR measurements. • After testing aggressive chemicals. • When calibration reminder is activated (“DO Cal Expired”). • If the readings are far from the calibration point.
Keep the protective cap on during polarization time and remove it for calibration and measurements. To calibrate the meter: • Insert and rinse the probe in the first beaker in order to decontaminate it; • Insert the probe in the second beaker; • Tap the probe repeatedly to remove any air bubbles that may be trapped inside the sleeve. • Enter in calibration mode by pressing • Wait to stabilize; ; • You can delete a previously performed calibration by pressing .
PRESSURE CALIBRATION If Automatic pressure source is chosen from the Pressure Source menu (see DO Setup), a pressure calibration in one point can be performed. To perform pressure calibration: • Press • Use • Press calibration.
DO MEASUREMENT Make sure the instrument has been calibrated before taking DO measurements. DIRECT MEASUREMENT To measure the DO of a sample using the Direct reading mode: and then to select DO measure mode. • Press • Select the Direct reading mode (see DO Setup). • Submerge the DO probe and tap it repeatedly to remove any air bubbles that may be trapped inside the sleeve. Allow time for the reading to stabilize. • The measured DO value will be displayed together with the temperature and pressure values.
SALINITY COMPENSATION If the sample contains significant concentration of salinity, the read out values must be corrected, taking into account the lower degree of oxygen solubility in this situation. Before taking any DO measurements remember to set the salinity value from the DO setup menu. The salinity affects the DO concentration, decreasing its value. The table below shows the maximum oxygen solubility at various temperatures and salinity levels. ºC Salinity (g/l) at Sea L evel 0 g/l 10 g/l 0 14.
BAROMETRIC PRESSURE COMPENSATION The dissolved oxygen saturation value varies with pressure, so it is important to compensate the effect that pressure has on DO measurements. Altitude, Meters above Sea L evel ºC 0 m 300 m 600 900 1200 1500 1800 2100 2400 2700 3000 3300 3600 3900 4000 m m m m m m m m m m m m m ºF 0 14.6 14.1 13.6 13.1 12.6 12.1 11.7 11.2 10.8 10.4 10.0 9.7 9.3 9.0 8.9 32.0 2 13.8 13.3 12.8 12.4 11.9 11.5 11.0 10.6 10.2 9.9 9.5 9.2 8.8 8.5 8.4 35.6 4 13.1 12.6 12.2 11.7 11.
The meter contains a built-in barometer, and it is able to automatically compensate for changes in barometric pressure. If another pressure value than the barometer’s reading is to be used, then the manual pressure feature must be enabled. (See DO Setup). The table below contains a conversion altitude (m) to pressure (mmHg) for the altitude values from the previous table.
BOD MEASUREMENT Biochemical oxygen demand (BOD) is an indicator for the concentration of biodegradable organic matter present in a sample of water. It can be used to determine the general quality of the water and its degree of pollution. BOD measures the rate of oxygen uptake by microorganisms in a sample of water at a fixed temperature and over a given period of time. To ensure that all other conditions are equal, a very small amount of microorganism seed is added to each sample being tested.
to display the BOD information screen corre• Press sponding to the selected bottle ID. • Press screen. to load the method data to the measurement • If the time difference between the current reading and the selected reading is more than 1 day, appears instead • Press and the BOD value can be calculated. to display the BOD value. • If there are at least two BOD values calculated then you can to enter the Select Bottle ID screen. press • Press to access the BOD Select View screen.
• From the Available Bottle ID list select the desired bottle ID or and press to add the using method to the Selected Bottle ID list. to delete a selected method from the Selected • Press Bottle ID list. • Press to switch between the two lists. to access • When in Selected Bottle ID list, press additionals options corresponding to the selected bottle ID. • Press for each sample to display the results containing the seed correction.
OUR MEASURE MENT The OUR is used to determine the oxygen consumption or respiration rate. It is defined as the mg/L of oxygen consumed per hour.
s SOUR MEASURE MENT The Specific Oxygen Uptake Rate (SOUR), also known as the oxygen consumption or respiration rate, is defined as the milligram of oxygen consumed per gram of volatile suspended solids (VSS) per hour. This quick measurement has many advantages: rapid measure of influent organic load and biodegradability, indication of the presence of toxic or inhibitory wastes, degree of stability and condition of a sample, and calculation of oxygen demand rates at various points in the aeration basin.
LOGGING This feature allows the user to log DO, BOD, OUR, SOUR and temperature. The logging behaviour is dependent on the Logging Type and Reading Mode (DO only) options from the parameter setup. The Logging Data Configuration options from the appropriate parameter setup must be set first in order to be saved into the log report.
to enter log interval edit menu and use • Press or to adjust the logging start-stop time or the log sampling. Press or • Press to save the current value and use to adjust next / previous parameter. to exit log interval edit menu and then press to save the current log. • While the instrument is saving the data, a “Please wait...” pop-up message will be displayed on the LCD. LOGGING MODE 2 (DO only) This logging mode can be used for multiple samples measurement.
LOGGING MODE 3 This logging mode can be used for any sample measurements. By will be available in Measure choosing this logging mode, mode. To log data using this mode: while in Measure mode to manually log a • Press record. The “Logged” indicator will be displayed on the LCD. • The records will be stored in one lot. In order to change the logging lot, see the measured parameter setup for details, Log option, New Lot generation. LOGGING MODE 4 This logging mode can be used for multiple samples measurement.
. • To stop the logging session, press Notes: • For the automatic logging, if the maximum logging time (24h) has been reached, a warning pop-up will be displayed on the LCD in order to save the current log and start another one in a new lot. • If 100 lots have been saved or maximum 10000 records have been manually stored, a warning pop-up will be displayed on the LCD in order to delete one lot or to select a new lot for the manual logging to log other records.
• Press to return to the previous menu. To delete lots: • Press while in Log Recall mode. • Press or mode. Otherwise, press mode. to access delete or delete all to return to Log Recall view • After selecting one of the deleting modes, use or to select one lot and then press or to delete the selected lot or all lots. The “Please wait...” message will be displayed on the LCD until the selected lot or all lots are deleted.
PC INTERFACE Data transmission from the instrument to the PC can be done with the HI 92000 Windows® compatible software (optional). HI 92000 also offers graphing and on-line help feature. Data can be exported to the most popular spreadsheet programs for further analysis. HI 4421 instrument has two available serial interfaces: RS232 and USB. The desired serial interface can be selected from the settings window of the HI 92000 software.
TROUBLESHOOTING GUIDE SYMPTOMS PROBLEM SOLUTION D isplay shows "-----" during measurements. Reading out of range. Recalibrate the meter; Check the sample is within the measurable range. The meter fails to calibrate or gives faulty readings. The probe is damaged. Replace the probe. The instrument doesn't measure the temperature from the probe. The probe temperature sensor is broken. Replace the probe. E xplicit warnings are displayed during calibration.
ACCESSORIES HI 7040M HI 7040L HI 7041S Zero Oxygen Solution, 230 mL Zero Oxygen Solution, 500 mL Refilling Electrolyte Solution, 30 mL PROBE CLEANING SOLUTIONS HI 7061M General Cleaning Solution, 230 mL bottle HI 7061L General Cleaning Solution, 500 mL bottle HI 8061M General Cleaning Solution, 230 mL FDA approved bottle HI 8061L General Cleaning Solution, 500 mL FDA approved bottle OTHER ACCESSORIES HI HI HI HI HI HI 76408 76407A/P 710005/8 710006/8 920010 92000 DO probe for laboratory use with built-i
RECOMMENDATIONS FOR USERS Before using these products, make sure they are entirely suitable for the environment in which they are used. Operation of these instruments in residential areas could cause unacceptable interferences to radio and TV equipment, requiring the operator to follow all necessary steps to correct interferences. During operation, ESD wrist straps should be worn to avoid possible damage to the probe by electrostatic discharges.
SALES AND TECHNICAL SERVICE CONTACTS Australia: Tel. (03) 9769.0666 • Fax (03) 9769.0699 China: Tel. (10) 88570068 • Fax (10) 88570060 Egypt: Tel. & Fax (02) 2758.683 Germany: Tel. (07851) 9129-0 • Fax (07851) 9129-99 Greece: Tel. (210) 823.5192 • Fax (210) 884.0210 Indonesia: Tel. (21) 4584.2941 • Fax (21) 4584.2942 Japan: Tel. (03) 3258.9565 • Fax (03) 3258.9567 Korea: Tel. (02) 2278.5147 • Fax (02) 2264.1729 Malaysia: Tel. (603) 5638.9940 • Fax (603) 5638.9829 Singapore: Tel. 6296.
