RapidWash User Manual Rev 1.
Index Section 1 - Setup ................................................................................................................. 3 1.1- Tubing and Connections .......................................................................................... 3 1.2 - Bottles .................................................................................................................... 4 1.2- RapidWash Select Manifold ....................................................................................
Section 1 - Setup 1.
Liquid 1 and 2 Dispense Bottle Tubing for Liquid 1 or 2 Pressure source Tubing to Liquid 1 or Liquid 2 Port on Select Manifold Liquid Level Sensor location Figure 2 Waste Bottle Waste Level Sensor location Tubing For Waste Port on Select Manifold Tubing to Vacuum Source Figure 3 1.
• • • Pressure In- From 12psi pressure source (#9 from figure 1) from the rear of the RapidWash to top of bottle (1/4” blue tubing-See Figure 2) Level Sensor - positioned near bottom of bottle (See Figure 2) Liquid Out - From bottle to side port of RapidWash Select Manifold (See Figure 4) Liquid 2 • Pressure In- From 12psi pressure source (#9 from figure 1) from the rear of the RapidWash to top of bottle (1/4” blue tubing-See Figure 2) • Level Sensor - positioned near bottom of bottle (See Figure 2) • Liq
RapidWash Select Manifold Figure 4 Figure 5 6
Section 2 - Operation of RapidWash through the Touch Screen Controller 2.
2.2 - Wash The RapidWash can support up to 20 dispense programs. Press the Next or Prev key (Figure 8) on the Touch Panel to select the program number you would like to setup.
Figure 9 Figure 10 Aspirate – Allows selection of: Depth - The distance in mm traveled down into the plate wells, measured from the top of the plate. Speed - % of full speed travel during the aspirate step (Figure 11).
Figure 11 Dispense – Select the liquid number (1 or 2) and the desired volume, in uL (Figure 12). Note: If the liquid (1 or 2) being dispensed has not been primed into the dispense head, the RapidWash will automatically perform a priming step prior to the first dispense step in the Wash Program being run. Subsequently, if only that liquid is dispensed in that Wash Program, the remaining dispense steps will execute without another priming action. Figure 12 Delay – Entered as seconds.
Figure 13 Example of Wash Program (Figure 14) Figure 14 All program values and steps are automatically saved to that program, as they appear on the screen in Figure 14.
2.3 - Prime The Prime feature allows you to select the liquid number and the total volume (in uL) dispensed through the dispense manifold. Priming occurs at the priming trough. During the prime process the trough will self empty to the waste bottle (Figure 15). Figure 15 Volume – This is the total volume that will be primed through the system.
2.4 - Auto Prime Auto Prime performs exactly like the Prime feature but will run automatically at the user determined interval (Figure 16) Volume – This is the total volume that will be primed through the system.
2.5 - Maintenance Mode The features in this section are used to clean and maintain the performance of the dispense and aspirate manifolds (Figure 17). Figure 17 Cleaning Procedure – This feature is used to flush the dispense manifold, it can be helpful when attempting to remove debris from the dispense manifold. During the cleaning procedure wash solution is dispensed out of the dispense manifold into a user supplied trough and aspirated back though the dispense manifold to waste.
Figure 18 Plate Height - Operates as described previously Cycles - The number of times the cleaning process will be performed Volume - This is the volume dispensed through the system: because the fluid path could be empty after your first cycle you should pay close attention to this volume to avoid under or over dispensing into your wash plate Liquid 1, Liquid 2 - Operates as described previously Soak Delay - Number of seconds the dispense manifold will soak in the dispensed liquid Drain Time - The number o
by the number of wells (i.e. a volume of 9600 would lead to 9.6 mL dispensed across a 96 well plate or 100 uL in every well). If run without the manifold attached, volume dispensed will not match what is defined due to a different flow rate without the head. On the run screen enter volume in uL and press “Run”. Manual Move – This feature is used to jog the dispense head (Y Axis) IN or OUT, jog the plate nest (Z Axis) UP or DOWN, and OPEN/CLOSE the plate gripper on track-based units.
2.6 – Change Settings This Section allows you to modify all the performance settings of the RapidWash (Figure 19) Figure 19 Settings Tab: Aspirate Shift- This is the distance that the RapidWash will use above and in the plate wells to begin the aspirate step. +mm is above the top of the Plate and –mm is aspirate begins in the plate. Dispense Shift- This is the distance that the RapidWash will use above and in the plate wells to begin the dispense step.
Initialize on Powerup- This will allow the RapidWash to automatically home when the unit is turned on. The default setting is auto initialize is set to on. Set Dispense Pressure- This option has been removed (12/10/2013). Calibration tab: This screen allows you to recalibrate the positions saved in the RapidWash (Figure 20). Figure 20 Prime Position- This is the saved position of the dispense manifold when it primes the RapidWash (Figure 21).
Figure 21 Aspirate Origin- This is the saved position of the aspirate manifold when the RapidWash begins to aspirate. This position is calibrated with a 96 well teaching plate. The position should be taught where each set of tips are centered in each well of the plate (Figure 22).
Dispense Origin- This is the saved position of the dispense manifold when the RapidWash begins to dispense. This position is calibrated with a 96 well teaching plate. The position should be taught where each set of tips are centered in each well of the plate (Figure 23). Figure 23 Track/Nest Height- This Position should only be modified when the unit is used with a TrackLink on our Lablinx system. This is the beginning position of the nest, default is 0.
The image cannot be display ed. Your computer may not hav e enough memory to open the image, or the image may hav e been corrupted. Restart y our computer, and then open the file again. If the red x still appears, y ou may hav e to delete the image and then insert it again. Figure 25 Plate Origin- This is the starting position (origin) of the aspirate tips relative to the teaching plate. To teach this position move the Aspirate head out until the front set of tips are aligned with the teaching plate.
Calibration tab: The validation setting turn on/off various checks that the unit can perform prior to executing programs and priming. Validation checks occur only on the resources needed for a particular operation (i.e. If liquid 1 is the only fluid being used, the unit will not validate liquid 2). It is strongly recommended that the validation checks are not disabled. - Check Waste Level: Checks if the sensor on the waste bottle is activated.
Section 3 - Maintenance of the RapidWash Manifold 3.1 - Removal and Installation of the Manifold The RapidWash is shipped without the manifold assembly attached to the unit. The manifold assembly is shipped with a shipping bracket to protect the tips. This shipping bracket must be removed before the Manifold assembly can be installed onto the RapidWash. The Manifold assembly has a quick disconnect fitting that holds the Manifold (Figure 27).
Figure 28 Slide the manifold onto the locating pins and continue to slide the manifold towards the mount until it locks into place (Figure 29). To remove the manifold press the Steel release tab on the top of the quick disconnect, then slowly slide the manifold off the locating pins on the manifold mount (Figure 29).
3.2 - Disassembly of the Manifold Remove the flat head screws that hold the aspirate and dispense manifolds together. If desired, the standoffs may be removed from the dispense manifold (Figure 30) Figure 30 3.3 - Cleaning All of the pump parts may be cleaned in an ultrasonic cleaner with a dilute detergent solution. The parts must be thoroughly rinsed prior to reassembly.
Section 4 - Hardware Specifications Section 4.1 - General NOTE: Specifications are subject to change without notice. RapidWash: • Plate Format: • Housing Material: • • Plate Nest Material Number of Axes 96, 384 well plates in shallow, medium or deep well-formatted SBS plate. Painted steel covering anodized cast aluminum housing Acetal Copolymer Two (Y and Z axes) Section 4.
Manifold: • • • Manifold Material: Sealing O-rings Fixed tip Material: Acetal Copolymer Viton Stainless Steel Section 4.3 - Dimensions: • • 12.34”H X 11.11”W X 14.98”D Weight – 10LBS Section 4.4 - Electrical: • • • Power Input: Fuses: Grounding: • Computer Interface: 120VAC/240VAC, 50/60Hz Two 2A time delay, 5mm x 20mm fuse Through the power cord, must be properly earth grounded RS-232 straight through serial cable Section 4.
