- Operator's Manual Aqua TROLL® 400 Instrument Information subject to change without notice. In-Situ, In-Situ logo, Baro Merge, BaroTROLL, HERMIT, iSitu, Pocket-Situ, RDO, RuggedCable, RuggedReader, TROLL, and Win-Situ are trademarks or registered trademarks of In-Situ Inc. © 2013. All rights reserved. 0088302 | Rev. 
- Copyright © 2012-2013 by In-Situ Inc. All rights reserved. This document contains proprietary information which is protected by copyright. No part of this document may be photocopied, reproduced, or translated to another language without the prior written consent of In-Situ Inc. Mailing and Shipping Address: In-Situ Inc. 221 East Lincoln Avenue Fort Collins, CO 80524 U.S.A. Phone: 970-498-1500 (international & domestic) Fax: 970-498-1598 Internet: www.in-situ.com Support: 800-446-7488 (U.S.A. 
- Table of Contents 1 Introduction 5 Scope Serial Number Location 5 5 2 Safety 5 Electrical Safety 5 3 General Specifications 4 Sensor Specifications 6 7 Level, Depth, Pressure Sensor Specifications Conductivity Sensor Specifications Dissolved Oxygen RDO Classic (Optical Sensor) Specifications ORP Sensor Specifications pH Sensor Specifications Sample Temperature Sensor Specifications (Probe) 5 Instrument Overview 7 7 8 8 9 9 10 Instrument Description 10 6 System Components 10 Probe Dimension 
- Conductivity Calibration 28 29 30 Pressure/Level pH/ORP Calibration 9 Controller Requirements and Connections Wiring Overview Stripped-and-Tinned Cable Power Connections SDI-12 Wiring Diagram Modbus Master RS485 Wiring Diagram Modbus Master RS232 Wiring Diagram (Converter Required) RS485 Network Guidelines DB-9 Diagram Communication Overview 10 Care and Maintenance 32 32 32 33 34 35 36 36 36 37 Maintenance Schedule User-Serviceable Parts O-rings RDO Classic Sensor Cap Replacement pH/ORP Sensor Replac 
- Introduction This manual is intended to describe the characteristics, operation, calibration, and maintenance of the Aqua TROLL 400 Instrument. Communication registers and programming information can be found in the Modbus and SDI-12 Reference Guide. Scope This manual covers the following information. 
- General Specifications Operating temperature -5 to 50° C (23 to 122° F) Storage temperature -40 to 65° C (-40 to 149° F) Dimensions 4.7 cm (1.85 in.) OD x 26.9 cm (10.6 in.) with restrictor installed (does not include connector) Weight 694 g (1.53 lbs) Wetted materials PVC, 316 stainless steel, titanium, Acetal, Viton®, PC/PMMA Environmental rating IP68 with all sensors and cable attached. IP67 with sensors removed and cable detached. 
- Sensor Specifications Level, Depth, Pressure Sensor Specifications Accuracy Typical ±0.1% FS @ 15° C; ±0.3% FS max. from 0 to 50° C Range 76 m (250 ft); absolute (non-vented) Resolution ±0.01% FS or better Sensor Type Fixed Response Time Instantaneous in thermal equilibrium Units of Measure Pressure: psi, kPa, bar, mbar, mmHg, inHg Level: mm, cm, m, in, ft Methodology Piezoresistive; ceramic Conductivity Sensor Specifications Accuracy Typical ±0.5% + 1 μS/cm; ±1% max. 
- Dissolved Oxygen RDO Classic (Optical Sensor) Specifications Accuracy ±0.1 mg/L; ±0.2 mg/L; ±10% of reading Range 0 to 8 mg/L; 8 to 20 mg/L; 20 to 50 mg/L; Full operating range: 0 to 50 mg/L Resolution 0.01 mg/L Sensor Type Fixed with replaceable RDO Classic Sensor Cap (life: 1 year typical) Response Time T90: <45 sec. T95: <60 sec. Units of Measure mg/L, % saturation, ppm Methodology EPA-approved In-Situ Methods 1002-8-2009 1003-8-2009 1004-8-2009 ORP Sensor Specifications Accuracy ±5. 
- pH Sensor Specifications Accuracy ±0.1 pH unit from 0 to 12 pH units Range 0 to 12 pH units Resolution 0.01 pH unit Sensor Type Replaceable pH/ORP combo sensor Response Time <15 sec., pH 7 to pH 4 Units of Measure pH units Methodology Std. Methods 4500-H+ EPA 150.2 Sample Temperature Sensor Specifications (Probe) Accuracy ±0.1° C Range -5 to 50° C (23 to 122° F) Resolution 0.01° C or better Sensor Type Fixed Response Time <30 sec. 
- Instrument Overview Instrument Description The Aqua TROLL 400 Instrument is a multiparameter water quality probe. The dissolved oxygen, conductivity, pressure, and temperature sensors are integrated into the instrument. The pH/ORP sensor and the RDO Sensor Cap are replaceable. The instrument is intended for use with a PLC/SCADA system or other data-logging device. It does not include internal power or an internal data logger. 
- Probe Dimensions with Restrictor On Total length with connector 296.49 mm (11.7 in.) Total length without connector 269.11 mm (10.6 in.) Restrictor length 118.24 mm (4.7 in.) Diameter 47 mm (1.85 in.) Probe Dimensions with Restrictor Off Sensor length 800-446-1500 81.09 mm (3.2 in.) 11 www.in-situ. 
- Sensors Sensors include optical RDO (Rugged Dissolved Oxygen), pH/ORP, conductivity, pressure, and temperature. 1 Pressure sensor 76 m (250 ft) 2 pH/ORP sensor 3 Conductivity sensor 4 Temperature sensor 5 RDO Sensor Cable The cable includes a twist-lock connection to the instrument and a stripped-and-tinned termination that must be wired to a controller. Cable length is customizable. Maximum length is 1,219 m (4,000 ft) for Modbus output, and 60.9 m (200 ft) for SDI-12 output. 
- Probe Setup The probe is shipped with a storage plug and protective dust caps in place. 1 Dust cap protector on the RDO Sensor. (Install the RDO Cap before deploying the instrument.) 2 pH/ORP storage plug. (Remove the storage plug and install the pH/ORP sensor before deploying the instrument.) 3 Dust cap protector on the twist-lock cable connector. 800-446-1500 13 www.in-situ. 
- Installing the Sensors 800-446-1500 14 www.in-situ. 
- 1. Twist the restrictor off the probe. 2. Locate the RDO Sensor Cap container and remove the cap. 3. Remove the dust cap from the RDO Sensor. 4. Align the flat edge of the RDO Sensor with the slotted edge of the RDO Cap and press the cap into position. Push until the cap is firmly in place. Important: Avoid touching the sensor lens and the sensing material on the top of the cap. 5. Remove the orange plug from the pH/ORP port. 6. Remove the pH/ORP sensor from the storage bottle. 
- Communication Settings and Calibration Before you program the instrument to work with your PLC/SCADA system, you must set appropriate communication settings using a TROLL Com, AC/DC converter, and WinSitu 5 Software. The software can also be used to calibrate sensors and to restore factory calibration and communication settings. Always wear appropriate personal protective equipment and use proper laboratory technique when calibrating the sensors and operating the instrument. 
- First Screen (Data Tab) When you open Win-Situ 5 Software, the Data tab appears. The left side of the screen contains a file tree where you can view data you have exported to Microsoft Office Excel. The disconnected plug icon in the lower-right corner of the screen indicates that the software is not yet communicating with an instrument. Screen Element Definition The disconnected plug indicates the instrument is not communicating with the software. 
- Screen Element Definition The Sensors tab lists the sensors in the connected instrument, along with their serial numbers and the dates of factory calibration and user calibration. Use the buttons in this tab to calibrate sensors that support user calibration and configure sensors that support configuration. The Device Setup tab allows access to instrument information and settings such as instrument name, serial number, firmware version, communication settings, diagnostics, and factory reset options. 
- Modbus Setup Click the Modbus setup button and assign instrument settings according to the requirements of your controller. For instrument Modbus registers, see the Modbus and SDI-12 Reference Guide. 800-446-1500 19 www.in-situ. 
- SDI-12 Setup SDI-12 setup allows you to set the instrument address, select the parameters you intend to log, and select the order in which the parameters will appear in your SCADA system or datalogger file. See the Help menu in Win-Situ 5 Software for details. To view SDI-12 programming information, see the Modbus and SDI-12 Reference Guide. 800-446-1500 20 www.in-situ. 
- View and Record Data The Home tab allows you to view data for the parameters that have been enabled. Gray values indicated that the instrument is not polling live data. To poll live data, click the Play button. Screen Element Definition The Sites button allows you to add, edit, or delete a site. Nonlogging instruments such as the Aqua TROLL 400 do not use sites. These icons allow you to view the memory and battery usage for an instrument that includes internal logging. 
- Screen Element Definition The Alarm icon provides additional instrument status information. Green—No alarms or warnings Yellow—One or more warnings Red—One or more alarms Move the cursor over the alarm icon to view a description. Click the Device Setup tab for detailed information on the alarm or warning. Note: Disregard the Device Reset alarm for non-logging instruments such as the RDO PRO-X Probe or the Aqua TROLL 400. System Time is displayed on the left. Device Time is displayed on the right. 
- Calibrate and Set Up Sensors The Sensors tab allows you to view the sensors that are available on the instrument. From this tab you can access calibration Wizards and sensor setup options. You can also view sensor serial numbers, factory calibration dates, and user calibration dates. Screen Element Purpose The Calibration button starts the Calibration Wizard for the selected sensor. This button opens the setup options for the selected sensor. 
- Set Parameter Units and Sentinel Values You can set sentinel values and set units for parameters by selecting a parameter and clicking the Setup Sensor button. Screen Element Purpose Parameter This menu lists the parameters that are available for the selected sensor. Units This drop-down list allows you to select units for the parameter you selected. Sentinel Offline Value This is a text field in which you can enter the value that you want to see in the data when a sensor is unable to communicate. 
- RDO Sensor Calibration Calibration 100% Oxygen Saturation 1. Place the calibration cap, with the vent hole, on the top of the calibration cup. 1 Storage cap 2 Calibration cap with vent hole 2. Place the sponge wafer in the bottom of the calibration cup and saturate the sponge wafer with approximately 10 mL clean water. 3. Gently dry the probe and sensing material with a paper towel. Ensure that the probe and the sensing surface are free of water and fouling. 4. 
- 6. In the software, select the Sensor Setup tab. 7. Select the RDO Dissolved Oxygen parameter. 8. Click Calibrate. 9. By default, 100% saturation is selected for the first point of the calibration. If you intend to perform a 2-point calibration, also select 0% saturation from the drop-down list. Otherwise, leave as “None.” 10. Click Next. 11. Enter the barometric pressure or elevation at which the instrument will be deployed. 12. Click Next. 13. Click OK to start the calibration. 14. 
- 5. Completely submerge the RDO Sensor into the solution. 6. Click OK, to start the calibration. 7. When the screen indicates that the calibration has reached stability, click Accept to complete the calibration, or click Cancel to return to the previous calibration. 8. You can save or print the calibration report. 9. Click OK to complete the calibration. 10. Once calibration is complete, remove the instrument from the calibration cup and rinse both thoroughly with clean water. 800-446-1500 27 www.in-situ. 
- Conductivity Calibration The conductivity sensor is calibrated with NIST-traceable standards at the factory, which provides a high degree of linearity across the entire operating range of 5 to 100,000 µS/cm. This sensor is capable of meeting its published specifications without requiring additional calibration by the user. Most commercially available standards can introduce a larger potential measurement error than the sensor’s initial factory calibration. 
- 6. Select the appropriate calibration standard from the drop-down list. If you select “User Defined,” enter the value of the solution. 7. Click Next. 8. Place the instrument into the calibration cup and allow time for the temperature to stabilize. 9. Gently tap the sides of the calibration cup against the palm of your hand to remove any bubbles in the conductivity cell. Visually inspect to ensure that all bubbles are removed. 10. Click OK to start the calibration. 11. 
- pH/ORP Calibration 1. Fill the calibration cup to the fill line with approximately 130 mL of the desired pH or ORP calibration solution. 2. Place the calibration cap on the instrument slightly above the restrictor, and place the instrument in the solution taking care to not force the solution out the top of the calibration cup. 3. In Win-Situ 5 Software, select the pH/ORP sensor. 4. Click the Calibrate button in the left corner of the screen. 800-446-1500 30 www.in-situ. 
- 5. Select either Calibrate pH or Calibrate ORP. 6. Click Next. 7. Select a value for the first calibration point. If you intend to perform a 2-point or 3point calibration, select the appropriate values as indicated on the label of the calibration standard. 8. Click Next. 9. Place the instrument into the calibration cup and allow time for the temperature to stabilize. 10. Click OK, to start the calibration. 11. 
- Controller Requirements and Connections The instrument can be connected to a controller or logger for communication via the following protocols. l SDI-12 l RS485 Modbus l RS232 Modbus (with converter) Wiring Overview Refer to the diagrams on the following pages. Trim and insulate unused wires. The shielded wire should be wired to a chassis ground or earth ground. See "Safety" on page 5. 
- SDI-12 Wiring Diagram Cable length must not exceed 60.9 m (200 ft). Signal Color Ground/Return Black External Power (9.6-16 VDC) Red SDI-12 White 800-446-1500 33 www.in-situ. 
- Modbus Master RS485 Wiring Diagram Cable length must not exceed 1,219 m (4,000 ft). Signal Color Ground/Return Black External Power (12-36 VDC) Red RS485 (-) Green RS485 (+) Blue 800-446-1500 34 www.in-situ. 
- Modbus Master RS232 Wiring Diagram (Converter Required) Cable length between Master and Slave must not exceed 1,219 m (4,000 ft). Cable length between Master and Converter must not exceed 6 m (20 ft). Signal Color Ground/Return Black External Power (12-36 VDC, voltage limited by converter) Red RS485 (-) Green RS485 (+) Blue 800-446-1500 35 www.in-situ. 
- RS485 Network Guidelines The instrument uses RS485 as its main digital communications link. RS485 is often used in an industrial setting as a small device network. There are some installation guidelines to follow when configuring an RS485 network with this instrument. See the Modbus and SDI-12 Reference Guide. 
- Care and Maintenance Maintenance Schedule For best results, send the instrument to the manufacturer for factory calibration every 12 to 18 months. User-Serviceable Parts The user-serviceable parts on the instrument include the O-rings, the pH/ORP sensor, and the RDO Sensor Cap. O-rings The instrument has several O-rings that can be maintained by the user in order to keep moisture from entering the instrument and damaging the electronics. 
- 1. Remove the pH/ORP sensor and place the orange pH port plug into the empty pH/ORP port to prevent any humidity from entering the probe. 2. Locate the sensor storage bottle in which the pH sensor was originally shipped. 3. Open the bottle and remove the O-ring. 4. Moisten the sponge inside the bottle with either a pH storage solution or a pH 4 solution. 5. Slide the O-ring onto the sensor, and then slide the bottle cap over the sensor as shown. 6. 
- Remove Protein-Like Material or Slimy Film l l Clean the sensor with warm water and mild soap. Soak the sensor in 0.1M HCI solution for 10 minutes and then rinse with deionized water. Note: After performing any of these cleaning methods, rinse the sensor with water and then soak overnight in pH 4 buffer. Cleaning the RDO Sensor Clean the Sensor Cap 1. Leave the cap on the sensor. 2. Rinse the sensor with clean water from a squirt bottle or spray bottle. 3. 
- Cleaning Procedure 1 Avoid damaging the plastic material of the conductivity cell. Gently scrub the conductivity cell with a soft swab and mild soap such as a dilute solution of dish detergent. The probe is shipped with polyurethane foam swabs for this purpose. You can also achieve good results using a gentle back-and-forth motion with a thin cotton pipe cleaner. If debris is still present, continue to Cleaning Procedure 2. If the sensor is clean, skip to the last step. 
- Declaration of Conformity 800-446-1500 41 www.in-situ.