CytoFuge®® 2 Cytocentrifuge System Operator’s Manual
Operator’s Manual CytoFuge® 2 Cytocentrifuge System Model Number M801-22 FOR IN VITRO DIAGNOSTIC USE Product Number CF02 - CytoFuge 2 for 100-240 VAC, 50/60 Hz StatSpin and CytoFuge are registered trademarks of StatSpin Inc., a Subsidiary of IRIS International, Inc. Copyright 1998, 2003 Printed in U.S.A.
Table of Contents i How to use this manual.............................................................................................ii Section 1..............................................................................................................1 Unpacking and Installation.......................................................................................1 Inspect Packaging.................................................................................................1 Verify Contents.......
How to use this manual This manual along with information contained on product labels and in package inserts should provide you with all the information you need to operate and maintain the CytoFuge® 2. Notes appear in italics to highlight information. When the information requires special attention, a caution symbol appears next to the italicized text.
Section 1 Unpacking and Installation Inspect Packaging The CytoFuge 2 and its accessories are delivered in one carton. If the cytocentrifuge or accessories have suffered any damage in transport, please inform your carrier immediately. NOTE: Save shipping carton and components to simplify return should service be required. Verify Contents The package contains: One CytoFuge 2 cytocentrifuge (Model No. M801-22) One Universal Switching Power Supply (Product No. 01-3553-001, APS Model No.
Section 2 System Overview Principle and Intended Use IVD For in vitro diagnostic use in concentrating cells onto a microscope slide. The CytoFuge 2 is a microprocessor-controlled cell preparation system that uses centrifugal force to deposit cells onto microscope slides.
Symbols and Definitions (cont.
Section 3 Sample Requirements/Preparation Overview The approximate cell concentration of the specimen should be established prior to slide preparation on the CytoFuge 2. Samples containing higher than optimal cell concentration will produce slides with cells too closely packed or overlapping. Samples containing too low a cell concentration will yield slides where cells are difficult to find, count, or examine.
Section 4 Operating Instructions Quick Start Instructions Details regarding each instruction can be found in the following sections. 1. 2. 3. 4. 5. 6. 7. Assemble the number and type of concentrators required. Install the concentrators in the rotor in a balanced arrangement. Load the concentrators with sample. (Empty concentrators may be used for balance.) Screw on the rotor lid. Close and latch cover. Select time and speed. Press start.
Access the Rotor Access the rotor cover by turning the knurled knob 3 (cover nut) counterclockwise while holding the rotor itself to prevent turning. (See Figure 1.) Rotor Gasket Rotor Hold-Down Nut (3-lobed) 3. Cover Nut 4. Cell Concentrator (shown in rest position) 5. Rotor Cover 6. Rotor 7. Bowl Gasket 8. CytoFuge Bowl 9. Motor 10. Rotor Mount 11. Rotor Indexing Pin 1. 2. Figure 1. Cross section of the CytoFuge 2 showing the bowl and rotor with the names and locations of the parts.
CAUTION - Addition of excess amounts of liquid (overfilling) to Cell or Filter Concentrator will result in the fluid being “spun out” during centrifugation. Spin the Sample CAUTION - Never operate the CytoFuge 2 without the rotor cover 5 in place. 1. 2. 3. 4. Screw on the rotor cover5. Close and latch the centrifuge cover. Select the time and speed (See Table 1) Press start button. Table 1: CytoFuge 2 Speed and Time Settings.
Section 5 Maintenance Overview StatSpin recommends that instrument operators perform periodic inspections and preventative maintenance on all StatSpin instruments. Contact StatSpin’s customer service department or distributor if, at any time, the instrument is not functioning properly. CAUTION - Unplug the CytoFuge 2 from the wall outlet before performing maintenance.
Troubleshooting No Power Light ↓ Is the power cord completely plugged into the wall? ↓ YES ↓ Is the wall outlet working properly? ↓ Is the barrel connector from the transformer completely plugged into the centrifuge? ↓ YES ↓ Contact StatSpin or an Authorized Repair Facility. → NO→ Plug power cord into the wall. → NO→ Try another wall outlet. → NO→ Plug the barrel connector into the centrifuge. → NO→ Completely close the cover. → NO→ Press the bowl gasket all the way down into the housing slot.
Appendix Appendix A - Specifications Product No. Model No. Speeds and Forces Cycle times Electrical Dimensions Environmental CF02 M801-22 600 rpm / 20 xg 700 rpm / 27 xg 850 rpm / 40 xg 1000 rpm / 55 xg 1300 rpm / 93 xg 1600 rpm / 140 xg 2200 rpm / 265 xg 4400 rpm / 1060 xg 2, 4, 6, 8, 10, and 30 minutes 24 Volts, DC, 1.7 amp. Includes switching power supply for 100-240 VAC, 50/60 Hz Diameter 6.8”/ 16.3 cm Height 5.3”/12.0 cm Weight 4.75 lbs/2.
References 1. 2. 3. NCCLS. “Protection of Laboratory Workers from Occupationally Acquired Infections; Approved Guideline-Second Edition.” NCCLS document M29-A2 [ISBN 1-56238-453-8]. NCCLS, 940 West Valley Rd, Suite 1400, Wayne, Pennsylvania 19087-1898 USA, 2001. CDC. Recommendations for prevention of HIV transmission in health care settings. MMWR (Suppl. No. 2S):2S-18S, 1987. CDC.
StatSpin Warranty StatSpin, Inc., a Subsidiary of IRIS International, Inc. warrants that the instruments shall be free from defects in material and/or workmanship, under normal use and service, for the period expiring twelve (12) months from the date of installation, provided the purchaser has completed and forwarded to StatSpin the Warranty Registration Card. StatSpin will, at its discretion repair or replace any unit covered under this warranty returned to StatSpin with shipping costs prepaid.
