User guide

17
5.5.1.2 Entering Known Calibration Curve Constants
The Use Standard Samples checkbox should be
clear and the Curve Fit option should be set to
the appropriate option. The known calibration
curve constants should then be entered into the
appropriate K0 – K3 fields where:-
Concentration = (K3*X
3
)+ (K2*X
2
)+ (K1*X) + K0
X = Photometric value of the sample
5.5.2 Constructing a New Calibration Curve
To construct a new calibration curve the user
must ensure that they check the tick boxes beside
the Calculate Concentration and Use Standard
Sample options.
5.5.2.1 Measuring Standard Samples
Select the Standard tab.
Insert the first standard into the sample position
in the sample chamber and the reference into the
reference position in the sample chamber.
Select the Start button to initiate the
measurement
A new window will open allowing the Standard
name, final photometric reading and
concentration to be edited. The actual
photometric reading of the standard will be
displayed in the Readout window and this value
will default to the deltaAbs field.