Spectrophotometers Models 7300 & 7305 Operating Manual 730 005 REV E /12-10
Safety Please read this information carefully prior to installing or using this equipment. 1. The unit described in this manual is designed be operated only by trained personnel. Any adjustments, maintenance and repair must be carried out as defined in this manual, by a person qualified to be aware of the hazards involved. 2. It is essential that both operating and service personnel employ a safe system of work, in addition to the detailed instructions specified in this manual. 3.
Contents Page Safety 3 Section 1 - Introduction 8 1.1 1.2 Instrument description Instrument specification Section 2 - Installation 2.1 2.2 2.3 2.4 2.5 2.6 Unpacking Installation Display Controls Rear panel Front panel 8 8 9 9 9 10 11 12 12 Section 3 - Theory and practice of spectroscopy measurements 13 3.1 3.2 3.3 13 13 14 Theory of spectroscopy measurement Spectroscopy measurement Good practice guidelines Section 4 - Instrument setup 16 4.1 16 17 17 18 18 18 4.2 4.3 4.4 4.5 4.
.3.2 6.4 6.4.1 6.4.2 Calibrating to a factor Sample measurement Measuring a sample after calibrating to a standard Measuring a sample after calibrating to a factor SECTION 7 – PRINTING AND AUTOLOGGING 26 7.1 7.1.1 7.1.2 7.2 7.2.1 7.2.2 7.3 26 26 27 27 27 28 28 Printing Print Setup Printing Results Autologging Setting the Number of Sample Repetitions Selecting Result’s Destination Connecting to a PC Section 8 - Accessories and spare parts 8.1 8.2 8.2.1 8.2.2 8.2.3 8.2.3.1 8.2.3.2 8.2.3.3 8.2.3.4 8.
Section 1 - Introduction 1.1 INSTRUMENT DESCRIPTION The 7300 and 7305 spectrophotometers are suited to a wide range of applications in education, quality control, environmental and clinical analysis. The 7300 is a visible spectrophotometer covering a wavelength range from 320nm to 1000nm. The 7305 is a UV/Visible spectrophotometer with a wavelength range from 198nm to 1000nm. Both models feature measurement modes for absorbance, % transmittance and concentration.
Section 2 - Installation 2.1 UNpacking Remove the 7300 or 7305 from the packaging and ensure the following items are included: 1. Model 7300 spectrophotometer (730 001), or Model 7305 spectrophotometer (730 501) 2. 24V 65W power supply unit (021 060) 3. Pack of 100 disposable plastic visible wavelength cuvettes (060 084), or pack of 100 disposable UV plastic cuvettes (060 230) 4. Jenway 73 series PC software (735 100) and interface cable (013 203) 5. Instruction manual (730 005) 6.
1 3 7300 2 4 09:02 Fig 2.2.2 – All Power On Tests Complete 1. Instrument check – ensures the validity of the saved parameters 2. Dark test 3. Checks for the accessory fitted. If an active accessory is found the instrument verifies communication and response 4. Self calibration of wavelengths 2.3 DISPLAY These spectrophotometers have a dot matrix display which enables icons and graphs to be displayed clearly.
2.4 CONTROLS The keypad used for these models enables an easy and effective way of navigating the different measurement modes, entering numbers, saving and analysing results. The soft keys are active when an icon is displayed above or adjacent to the key. The only exception to this is the back key which is always active. The main menu screen and surrounding keypad is displayed below. 7300 1 5 1 5 09:02 2 3 4 Fig. 2.4.1 – Display 1. Photometrics measurement mode 2. Back key 3. Time and date menu 4.
2.5 REAR PANEL The image below shows the rear panel on the instrument: 2 3 4 5 1 Fig. 2.5.1 – Rear Panel 2.6 1. Lamp access panel Allows access to lamp when replacement is necessary 2. Power switch On/off switch for the unit 3. Power in socket Connection socket for power supply unit 4. RS232 serial port Connection to a PC or external serial printer 5. Output sockets Analogue output FRONT PANEL The image below shows the front panel of the instrument: 1 2 4 3 Fig. 2.6.1 – Front Panel 1.
SECTION 3 – Theory and Practice of Spectroscopy Measurements 3.1 THEORY OF SPECTROSCOPY MEASUREMENT UV-visible spectroscopy is the measurement of the absorbance of light at a specific wavelength in a sample. This is used to identify the presence and concentration of molecular entities within the sample. The Beer-Lambert law is used to relate the absorption of light to the properties of the sample through which the light is travelling through.
The optical layout of the 7300 and 7305 spectrophotometers is shown below: Lamp Entrance slit Grating Collimator mirror Exit Slit Detector Sample Collecting Lens Figure 3.2.1 – Diagram of light path The light from the pre-focused tungsten halogen (7300) or pre-aligned xenon (7305) lamp is focused onto the grating, with 1200 lines per millimeter, which separates the light into discreet wavelengths.
inner chamber (the area filled with sample) must be wider than the aperture in the sample holder or light will reach the detector without passing through the sample. In this case, semi-micro or micro cuvettes with self-screening black surrounds must be used or, alternative holders for these cuvettes should be used. e) Glass test tubes and other sample tubes should be used with care. Where possible, matched tubes should be used and any index mark set to the correct position before measurements are made.
SECTION 4 – Instrument Setup 4.1 NAVIGATING AND SCREEN SETUP The main menu screen is displayed below. 7300 Photometrics measurement mode Concentration measurement mode 09:02 Instrument settings menu Back key Time and date menu Fig 4.1.1 – Home Screen To navigate around the spectrophotometer screen press the soft keys adjacent to icons displayed on the screen. In the main menu either of the two soft keys adjacent to the measurement mode icon can be pressed to access the mode.
The measurement settings can be accessed through the utility toolbar displayed on the left hand side of the expanded operating menu. This toolbar provides the same functions in all of the measurement modes. The utility toolbar enables access to printing, print setup options and autologging options. For more details on the different functions of the utility toolbar refer to section 7. 4.2 TIME AND DATE The time and date menu enables the current time and date to be set.
4.4 DIAGNOSTICS 0.00 Go to WL 500.0 nm ON 4.5 READ The diagnostic function allows simple checks to be carried out on the instrument. The wavelength can be changed, the lamp can be turned on and off and a sensitivity reading can be performed. To exit this function without performing any checks press the back key. SCREEN CONTRAST The screen contrast function enables the brightness of the screen to be set. In the instrument settings menu press the key adjacent to the screen contrast icon.
0.000 100.0 ABS %T 400 nm The lamp and fan will be turned back on and the lamp will begin to warm up. The lamp cold icon is displayed adjacent to the calibrate to zero icon in the measurement mode. The time needed for the lamp to warm up is five minutes. 09:02 0.000 100.0 ABS %T Once the warm up time of five minutes is complete the lamp cold icon disappears.
SECTION 5 – Photometrics The photometrics measurement mode enables simple measurements of absorbance and % transmittance to be performed. The sample is measured at one wavelength and at one point in time. There are no post measurement calculations available in this measurement mode. 5.1 MODE SPECIFIC PARAMETERS 0.000 100.0 The photometrics minimal operating menu enables calibration to zero absorbance/100% transmittance and simple readings to be taken without changing any measurement parameters.
The toggle icon enables the large primary display to be set to show the absorbance or % transmittance. To change the primary and secondary displays press the key adjacent to the toggle icon. Repeat presses will cycle the displays between absorbance or % transmittance. 5.2.1 Selecting a Wavelength The wavelength can be adjusted in the expanded operating menu by using the keys adjacent to the arrow icons to increase or decrease the wavelength.
SECTION 6 – Concentration The concentration measurement mode enables simple measurements of absorbance and concentration to be performed. In this measurement mode it is possible to calibrate against a standard of a known concentration or use a known factor. The sample is measured at one wavelength at one point in time. There are no post measurement calculations available in this measurement mode. 6.1 MODE SPECIFIC PARAMETERS 0.000 0.
2.008 The settings menu is accessed through the expanded operating menu by pressing the key adjacent to the settings icon. In the settings menu press the key below the wavelength icon. 1.000 ppm 400nm 4 0 0 0 This will open a number entry screen. Use the keys at the bottom of the screen to select the digit to be adjusted. Use the keys adjacent to the arrow icons to increase or decrease the wavelength to the required number.
6.2.2.2 Changing the Resolution The resolution that the concentration is displayed as can be selected from 1, 0.1, 0.01 or 0.001 by repeat presses of the key below the resolution icon in the settings menu. 6.2.2.3 Using a Standard 000 0 00 00 1.0 The standard menu enables the value of a standard to be entered. This function is accessed by pressing the key adjacent to the standard icon. This opens the extended number entry screen.
0.000 0.000 ppm ABS 600 nm 0.000 ABS 20.000 Press the key below the calibrate to zero absorbance or standard icon, this will open another menu with the option to re-calibrate to zero absorbance or to calibrate to the previously entered standard value. Press the key adjacent to the calibrate to standard icon. If the standard selected requires a factor beyond the range of the instrument the check standard icon will be displayed. 09:02 20.00 0.
SECTION 7 – Printing and Autologging The utility toolbar in the expanded operating menu provides access to printing, print setup options, and autologging options. 0.000 Print/print settings 0.000 Autolog menu 0002x 003s ppm ABS 600 nm 09:02 Fig 7.1 - Expanded Operating Menu 7.1 PRINTING 0.000 0.000 ppm ABS The utility toolbar in the expanded operating menu enables results to be printed and print setup options to be set.
7.1.2 PRINTING RESULTS 0.132 73.8 ABS %T 400 nm 0 9 : 02 7.2 AUTOLOGGING 0.000 0.000 ppm ABS 600 nm 0002x 003s 09:02 7.2.1 Results displayed in the expanded operating menu can be printed by pressing the key adjacent to the printer icon. Depending on the printout destination previously selected the result will be sent to the internal printer or the external printer.
7.2.2 Selecting Result’s Destination The autolog menu enables the result’s destination to be set. To select the internal printer press the key adjacent to the printer icon. This option is only available if an internal printer is connected. To send the results to an external instrument such as a PC or a serial printer press the key adjacent to the computer icon. X 0005 7.
SECTION 8 – Accessories and Spare Parts 8.1 OPTIONAL ACCESSORIES 8.
Squeeze the grey plastic clips together so that the printer top opens. Slot the printer into the top of the instrument and push down until it fits flush to all four sides. Insert the paper roll into the printer – ensuring that there is some paper sticking out of the printer before clicking the grey plastic back into place. Switch the instrument on. The power and error lights on the printer will flash.
8.2.3.1 Automatic 8 cell turret Take the 8 cell turret base plate. Connect the power supply in the bottom of the sample chamber to the connector on the underside of the baseplate. Place the base plate in the sample chamber. Replace screws 1 to 4. Take the 8 cell carousel and place on top of the motor, taking care to align the three ball bearings with the grooves on the motor shaft. Gently push the carousel down onto the motor shaft until it is located into place.
8.2.3.3 Sipper pump 6. For this accessory as well as removing the passive accessory base plate, the front panel of the instrument must also be removed. Loosen screws 5 and 6 until the front panel can be lifted out in a forward direction. 5. Take the sipper base plate. Connect the power supply in the bottom of the sample chamber to the connector on the underside of the base plate. Place the base plate in the sample chamber. Replace screws 1 to 4.
7. Cut a small length of the sipper pump tube and push this over one end of the capillary tube. Connect this to the inlet port of the flow-through cuvette. 8. Route the tube into the two retaining clips located on the base plate at the side of the pump head. 9. Fit the sipper probe and secure using the thumbscrew. Feed the capillary tubing through the tube and up through the sipper probe, allowing sufficient length for it to pass into a suitable receptacle. For pumping: 7. 1. 3. 4. 1.
8.3 USING THE ACCESSORIES 8.3.1 Automatic 8 cell turret 0.000 0.000 When the automatic 8 cell turret is in use the 8 cell turret icon is displayed in the bottom right hand corner of the screen. The current cell position is displayed adjacent to the 8 cell turret icon. The 0 position should always be used for the zero calibration sample. ppm ABS 500 nm 0 09:02 To perform measurements using the automatic 8 cell turret, insert the cuvettes containing the samples into turret positions 1 to 7.
8.3.3 Sipper pump 0.000 100.0 ABS %T 400 nm 09:02 8.3.3.1 When the sipper is in use the sipper pump icon is displayed in the bottom right hand corner of the screen. The pump direction is displayed by an arrow icon below the sipper pump icon. The sipper pump can operate in manual or timed mode, depending on the option selected in sipper pump settings. To open the sipper pump settings hold the key below the sipper pump icon for 2 seconds.
8.3.3.2 TIMED SIPPER PUMP SETTINGS To operate the sipper pump in timed mode press the key adjacent to the timed sipper pump icon. Press the key below the calibrate timed sipper icon. Select the required pump direction by pressing the key below the forwards or backwards arrow icon. Press the key adjacent to the tick icon to continue to the next stage of the calibration sequence. Insert the inlet tubing into the sample container and press the key adjacent to the single greater than icon.
To program an air gap remove the inlet tubing from the sample container and press the key adjacent to the single greater than icon. The sipper pump will start and air will be pumped through the tubing to the flow through cell. Once the required amount of air has been taken up press the key adjacent to the stop icon. The time taken for air uptake is recorded. To fine tune the amount of air uptake press the keys below the plus or minus icons to increase or decrease the amount of air taken in.
Once the measurement has been performed remove the tubing from the sample and press the key below the sipper pump icon to perform the next stage of the calibration sequence. 0.000 100.0 Confirmation will be needed to start the sipper pump. Press the key adjacent to the cross icon to cancel and return to the expanded operating menu. Press the key adjacent to the tick icon to confirm and start the sipper pump. The pump will run for the previously recorded air gap take up time.
8.
SECTION 9 – Maintenance and Service 9.1 ROUTINE MAINTENANCE Ensure the external surfaces of the unit are clean and free from dust. The sample area should always be kept clean and any accidental spillage should be wiped away immediately. To give added protection when not in use, the unit should be disconnected from the mains supply and covered with the optional dust cover. The only routine maintenance which maybe required is the replacement of the light source.
9.3 SERVICE Our dedicated service staff are on hand to help in the unlikely event that your Jenway equipment develops a fault. Please contact them by one of the following means with a clear description of the problem: E-mail: service@bibby-scientific.com Tel: +44 (0) 1785 810475 Fax: +44 (0) 1785 810471 On occasion it may be necessary for your equipment to be sent back to our Service Department for repair.
SECTION 10 – Troubleshooting 10.1 ERROR CODES If an error code is displayed it will be accompanied by a spanner icon and a symbol to indicate if the error is a warning (caution icon) or fatal (stop icon). If the error is fatal contact your local distributor or Jenway service department (refer to section 9.3). If the error is a warning it may be possible to retry the test. In this case a back icon will also be displayed.
Err 5 Light Saturation Not Found This error indicates that the peak light hasn’t been found at zero. The most likely causes of this error are: Fatal 1. Lamp failure. 2. Deterioating lamp signal. 3. Sample or cuvette in the sample holder. Solution: Ensure that the sample holder is empty. Restart the unit, if the problem persists contact a service technican.
10.2 TROUBLESHOOTING GUIDE Issue Solution Unable to achieve zero absorbance or 100% transmittance when calibrating Ensure that there is not a sample in the sample chamber. Ensure the instrument lid is closed before and during the calibration. Ensure the lamp is working – if the lamp has failed replace the lamp (7300) or lamp module (7305). Unable to achieve a reading Ensure the correct cuvette is being used so that light isn’t being when measuring a sample absorbed by the cuvette.
SECTION 11 – Declaration of Conformity 45
SECTION 11 – Declaration of Conformity 46
SECTION 12 – Glossary of Icons Mode Icon Description Common Back key Common Tick icon - Done/yes Common Cross icon – Cancel/no Common JW icon - Opens expanded operating menu Common Printer icon - Print/open printer settings Common No results to send to printer Common Computer icon - RS232 serial port for connection to an external serial printer or a computer Common No results to send to RS232 Common English English icon - Language selection Common Arrow icon - Res
Common Stop icon – accompanied by error code Main Menu Opens concentration measurement mode Main Menu Opens photometrics measurement mode Main Menu Instrument settings Main Menu 48 12.
Concentration Concentration Standard menu 000 Resets standard or factor value to zero Concentration Check standard Accessories No single cell - Method created on a unit with a single cell accessory fitted Accessories 8 cell turret icon – the automatic 8 cell turret is in use Accessories Error finding cell position 0 on turret Accessories No turret - Method created on a unit with a turret accessory fitted Accessories Accessories °C °F Accessories Degrees Celsius Degrees Fahrenhe
Accessories Sipper peltier pump in use Accessories No sipper peltier - Method created on a unit with a sipper peltier accessory fitted Accessories PC comms - shown when the instrument is connected to a PC and communicating via the PC software Error Spanner - Refer to section 10
INDEX ACCESSORIES Accessories - connecting Accessories - using AUTOLOGGING CONCENTRATION CALIBRATION METHOD SETUP MODE SPECIFIC PARAMETERS SAMPLE MEASUREMENT Connecting to a PC Controls Display Keypad Rear panel Declaration of conformity Error codes Good practice guidelines Icon GLOSSARY Installation Unpacking Instrument setup Contrast Expanded operating menu GLP settings Lamp save Navigating Utility toolbar Lamp replacement Tungsten halogen lamp replacement Xenon lamp module replacement Language PHOTOME
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