Spectrophotometers Models 7310 & 7315 Operating Manual 731 005 REV E/12-10
Safety Please read this information carefully prior to installing or using this equipment. 1. The unit described in this manual is designed be operated only by trained personnel. Any adjustments, maintenance and repair must be carried out as defined in this manual, by a person qualified to be aware of the hazards involved. 2. It is essential that both operating and service personnel employ a safe system of work, in addition to the detailed instructions specified in this manual. 3.
Contents Page Safety 3 Section 1 - Introduction 1.1 Instrument description 1.2 Instrument specification 8 8 8 Section 2 - Installation 2.1 Unpacking 2.2 Installation 2.3 Display 2.4 Controls 2.5 Rear panel 2.6 Front panel 12 12 12 13 14 15 15 Section 3 - Theory and practice of spectroscopy measurements 3.1 Theory of spectroscopy measurement 16 3.2 3.3 Spectroscopy measurement Good practice guidelines 16 16 17 Section 4 - Instrument setup 4.
.2.2.4 6.3 6.3.1 6.3.2 6.4 6.4.1 6.4.2 6 Using a factor Calibration Calibrating to a standard Calibrating to a factor Sample measurement Measuring a sample after calibrating to a standard Measuring a sample after calibrating to a factor 29 29 29 30 30 30 30 Section 7 - Spectrum 7.1 Mode specific parameters 7.2 Method setup 7.2.1 Scan settings 7.2.1.1 Selecting absorbance or % transmittance 7.2.1.2 Setting start and end wavelengths 7.2.1.3 Setting the scan interval 7.2.1.
9.2.1.9 9.3 9.4 9.5 Setting the kinetics measurement time Calibration Sample measurement Data analysis 48 48 48 49 Section 10 - Saving, printing and autologging 10.1 Saving methods 10.1.1 Saving methods to internal memory 10.1.2 Saving methods to USB memory stick 10.2 Opening methods 10.2.1 Opening methods from internal memory 10.2.2 Opening methods from USB memory stick 10.3 Deleting methods 10.4 Saving results 10.5 Opening results 10.6 Deleting results 10.7 Printing 10.
12.2.2 12.3 8 Xenon lamp module replacement Service 71 72 Section 13 - Troubleshooting 13.1 Error codes 13.2 Troubleshooting guide 13.
Section 1 - Introduction 1.1 INSTRUMENT DESCRIPTION The 7310 and 7315 spectrophotometers are suited to a wide range of applications in education, quality control, environmental and clinical analysis. The 7310 is a visible spectrophotometer covering a wavelength range from 320nm to 1000nm. The 7315 is a UV/Visible spectrophotometer with a wavelength range from 198nm to 1000nm. Both models have five measurement modes: photometrics, concentration, spectrum scanning, quantitation and kinetics.
7310 7315 Spectrum Range 320 to 1000nm 198 to 1000nm Scan interval Selectable 1, 2 or 5nm Analysis Absorbance or % transmittance and peak and valley wavelengths Other Beam height 15mm Light source Tungsten halogen lamp Xenon lamp Lamp save Yes Not applicable GLP Current time and date, user ID, settings lock and method lock Number of users 999 Methods memory 48 in each measurement mode Results memory Limited by attached mass storage device Removable media USB (supplied) Outputs
Section 2 - Installation 2.1 UNpacking Remove the 7310 or 7315 from the packaging and ensure the following items are included: 1. Model 7310 spectrophotometer (731 001), or Model 7315 spectrophotometer (731 501) 2. 24V 65W power supply unit (021 060) 3. Pack of 100 disposable plastic visible wavelength cuvettes (060 084), or pack of 100 disposable UV plastic cuvettes (060 230) 4. 2 GB USB memory stick (019 146) 5.
1 3 7310 2 4 09:02 5 Fig 2.2.2 – All Power On Tests Complete 1. Instrument check – ensures the validity of the saved parameters 2. Dark test 3. Checks for the accessory fitted. If an active accessory is found the instrument verifies communication and response 4. Self calibration of wavelengths 5. Checks communication between USB memory stick port and the instrument 2.3 DISPLAY These spectrophotometers have a dot matrix display which enables icons and graphs to be displayed clearly.
2.4 CONTROLS The keypad used for these models enables an easy and effective way of navigating the different measurement modes, entering numbers, saving and analysing results. The soft keys are active when an icon is displayed above or adjacent to the key. The only exception to this is the back key which is always active. The main menu screen and surrounding keypad is displayed below. 8 1 7310 1 2 8 7 2 7 09:02 3 4 5 Fig. 2.4.1 – Display 1. Spectrum measurement mode 2.
2.5 REAR PANEL The image below shows the rear panel on the instrument: 2 3 4 5 1 Fig. 2.5.1 – Rear Panel 1. Lamp access panel Allows access to lamp when replacement is necessary 2.6 2. Power switch On/off switch for the unit 3. Power in socket Connection socket for power supply unit 4. RS232 serial port Connection to a PC or external serial printer 5. Output sockets Analogue output FRONT PANEL The image below shows the front panel of the instrument: 1 2 5 4 3 Fig. 2.6.
SECTION 3 – Theory and Practice of Spectroscopy Measurements 3.1 THEORY OF SPECTROSCOPY MEASUREMENT UV-visible spectroscopy is the measurement of the absorbance of light at a specific wavelength in a sample. This is used to identify the presence and concentration of molecular entities within the sample. The Beer-Lambert law is used to relate the absorption of light to the properties of the sample through which the light is travelling through.
The optical layout of the 7310 and 7315 spectrophotometers is shown below: Lamp Entrance slit Grating Collimator mirror Exit Slit Detector Sample Collecting Lens Figure 3.2.1 – Diagram of light path The light from the pre-focused tungsten halogen (7310) or pre-aligned xenon (7315) lamp is focused onto the grating, with 1200 lines per millimeter, which separates the light into discreet wavelengths.
inner chamber (the area filled with sample) must be wider than the aperture in the sample holder or light will reach the detector without passing through the sample. In this case, semi-micro or micro cuvettes with self-screening black surrounds must be used or, alternative holders for these cuvettes should be used. e) Glass test tubes and other sample tubes should be used with care. Where possible, matched tubes should be used and any index mark set to the correct position before measurements are made.
SECTION 4 – Instrument Setup 4.1 NAVIGATING AND SCREEN SETUP The main menu screen is displayed below. Kinetics measurement mode Spectrum measurement mode 7310 Photometrics measurement mode Concentration measurement mode 09:02 Instrument settings menu Back key Time and date menu Quantitation measurement mode Fig 4.1.1 – Home Screen To navigate around the spectrophotometer screen press the soft keys adjacent to icons displayed on the screen.
The measurement settings can be accessed through the utility toolbar displayed on the left hand side of the expanded operating menu. This toolbar provides the same functions in all of the measurement modes. The utility toolbar enables access to printing, print setup options, opening, saving and deleting results and methods and autologging options. For more details on the different functions of the utility toolbar refer to section 10. 4.
4.4 SECURITY AND SETTING PASSWORDS 4.4.1 Setting Security Codes The security code function enables a security code to be set to lock the instrument settings and measurement mode settings. The security code is not specific to the user ID but is designed to enable an administrator to control either the instrument or protocols. The security code menu is accessed through the instrument settings menu. 6 4.4.2 6 0 In the instrument settings menu press the key adjacent to the security code icon.
One press will lock the methods instantly. To unlock the methods press the key adjacent to the method lock icon again. The methods are now unlocked. If the settings lock is active this must be disabled before the method lock can be activated or deactivated. In all the measurement modes if a user tries to save changes to a method when the method lock is active the padlock icon flashes on the screen and changes cannot be saved. 4.
0 4.8 0 1 The user ID function can be accessed through the instrument settings menu by pressing the key adjacent to the user ID icon. Use the keys at the bottom of the screen to select the digit to be changed. Use the keys adjacent to the arrow icons to increase or decrease the number. Once the preferred user ID has been set press the key adjacent to the tick icon to save and return to the instrument settings menu.
0.000 100.0 ABS %T 400 nm 09:02 0.000 100.0 ABS %T The time set will begin to count down when there is no lamp activity. When the count down is complete the lamp and the fan will be turned off and the lamp save icon is shown in all the measurement modes. To bring the instrument out of the lamp save in order to perform a measurement press the key below the lamp save icon. The lamp and fan will be turned back on and the lamp will begin to warm up.
SECTION 5 – Photometrics The photometrics measurement mode enables simple measurements of absorbance and % transmittance to be performed. The sample is measured at one wavelength and at one point in time. There are no post measurement calculations available in this measurement mode. 5.1 MODE SPECIFIC PARAMETERS 0.000 100.0 The photometrics minimal operating menu enables calibration to zero absorbance/100% transmittance and simple readings to be taken without changing any measurement parameters.
The toggle icon enables the large primary display to be set to show the absorbance or % transmittance. To change the primary and secondary displays press the key adjacent to the toggle icon. Repeat presses will cycle the displays between absorbance or % transmittance. 5.2.1 Selecting a Wavelength The wavelength can be adjusted in the expanded operating menu by using the keys adjacent to the arrow icons to increase or decrease the wavelength.
SECTION 6 – Concentration The concentration measurement mode enables simple measurements of absorbance and concentration to be performed. In this measurement mode it is possible to calibrate against a standard of a known concentration or use a known factor. The sample is measured at one wavelength at one point in time. There are no post measurement calculations available in this measurement mode. 6.1 MODE SPECIFIC PARAMETERS 0.000 0.
2.008 The settings menu is accessed through the expanded operating menu by pressing the key adjacent to the settings icon. In the settings menu press the key below the wavelength icon. 1.000 ppm 400nm 4 0 0 0 This will open a number entry screen. Use the keys at the bottom of the screen to select the digit to be adjusted. Use the keys adjacent to the arrow icons to increase or decrease the wavelength to the required number.
6.2.2.2 Changing the Resolution The resolution that the concentration is displayed as can be selected from 1, 0.1, 0.01 or 0.001 by repeat presses of the key below the resolution icon in the settings menu. 6.2.2.3 Using a Standard 000 0 00 00 1.0 The standard menu enables the value of a standard to be entered. This function is accessed by pressing the key adjacent to the standard icon. This opens the extended number entry screen.
0.000 0.000 ppm ABS 400 nm 0.000 ABS 20.000 Press the key below the calibrate to zero absorbance or standard icon, this will open another menu with the option to re-calibrate to zero absorbance or to calibrate to the previously entered standard value. Press the key adjacent to the calibrate to standard icon. If the standard selected requires a factor beyond the range of the instrument the check standard icon will be displayed. 09:02 10.00 0.
SECTION 7 – Spectrum The spectrum measurement mode enables measurements of absorbance or % transmittance over a range of wavelengths to be performed. The absorbance or % transmittance at each wavelength is plotted graphically. Post measurement tools such as peaks and valleys analysis and spectral points analysis can be performed. This operating mode can be used to partially characterise a sample. 7.
7.2 METHOD SETUP In this measurement mode all of the method setup parameters are accessed through the scan settings menu. To open the scan settings menu press the key adjacent to the scan settings icon in the expanded operating menu. 2.500 ABS -0.500 350 450nm 550 09:02 7.2.1 Scan Settings This function enables the graph y-axis, absorbance or % transmittance operating mode, start and end wavelengths and scan interval to be set.
If the start wavelength entered is the same as the end wavelength the end wavelength will automatically be set to be one times the scan interval. For example, if the start wavelength is entered as 500nm but the end wavelength is already set to 500nm and the scan interval is 2nm, the end wavelength will be automatically adjusted to 502nm. If the end wavelength entered is the same as the start wavelength the start wavelength will automatically be set to be one times the scan interval.
+ 7.3 2 .3 0 0 To change the maximum absorbance or % transmittance value press the key adjacent to the maximum y-axis value, this opens a number entry screen. Use the keys adjacent to the arrow icons to increase or decrease the number. To change the sign press the key below the + or - icon. Repeat presses will cycle between + and –. Once the required absorbance or % transmittance value has been entered press the key adjacent to the tick icon. CALIBRATION 2.500 ABS 0 100 -0.
To stop the scan before completion press the key below the scan in progress icon. Confirmation will be needed to stop the sample scan. Press the key adjacent to the cross icon to continue with the scan of the sample or press the key adjacent to the tick icon to confirm stopping the scan. 2.500 ABS -0.
7.5.2 Peaks and Valleys Table nm 425 450 475 1.500 ---1.500 nm 425 475 ---1.000 ---- In the peaks and valleys table screen it is possible to display both peaks and valleys, just peaks or just valleys. To display the peaks only press the key below the peak only icon. To redisplay the peaks and valleys press the same key again. ABS 1.500 1.500 nm 450 This function displays all the detected peaks and valleys above the selected threshold, in tabular form.
0 5 0 The wavelength to be analysed can also be entered manually. This can be done in two ways; firstly by pressing the key below the wavelength icon in the spectral points analysis menu. This opens a number entry screen where the wavelength can be input manually. Use the keys at the bottom of the screen to select the digit to be changed and use the keys adjacent to the arrow icons to increase or decrease the number.
SECTION 8 – Quantitation The quantitation measurement mode enables sample concentrations to be calculated using a standard curve. In this mode a number of standard solutions covering a range of known concentrations are measured at a set wavelength. The absorbance or % transmittance of these solutions is plotted to create a standard curve. Once the standard curve has been created a sample of unknown concentration can be measured and the concentration calculated using the standard curve. 8.
8.2 METHOD SETUP 8.2.1 Selecting a Wavelength -0.000 0.000 The wavelength can be changed in the expanded operating menu by using the keys adjacent to the arrow icons. Use the keys to increase or decrease the wavelength until the required wavelength has been selected. The wavelength selected should be the same wavelength that the standards and unknown samples are to be measured at. The wavelength can also be adjusted in the standard curve screen. Refer to section 8.2.3 for more details.
8.2.2.3 Changing the Resolution The resolution of the concentration can be selected from 1, 0.1, 0.01 or 0.001 by repeat presses of the key below the resolution icon. 8.2.2.4 Selecting Absorbance or % Transmittance The operating mode can be changed between absorbance or % transmittance by pressing the key below the Abs or %T icon. Repeat presses will cycle between absorbance or % transmittance. 8.2.2.5 Adding Standards 1 2 3 0.10 0.2 0 0.3 0 0 . 100 0 . 200 0 . 300 ppm + ABS 0 0 00 .
0.600 ABS 0.300 400 nm 0.000 0.00 0.30 ppm 0.60 y = mx+c When the current standard curve is displayed the concentration and photometric value of the last sample measured is also displayed on the curve. The curve fit algorithm can be changed by pressing the key below the curve fit icon. Repeat presses of the key cycles the curve fit between linear, linear through zero, quadratic, quadratic through zero and interpolate.
Once this measurement has been performed the standard concentration samples can be measured. Remove the cuvette containing the blank solution from the sample chamber and insert the cuvette containing the first standardised solution to be measured. Close the instrument lid and press the key adjacent to the tick icon to take a reading of the standard. The concentration along with the photometric value will then be displayed. This standard can be re-measured by pressing the key adjacent to the back icon. 0.
8.5 DATA ANALYSIS 0.600 ABS Y = mX+C m = c = r2 = 0.300 1. 000 0. 000 1. 149 0.000 0.00 y = mx+c 0.30 ppm 0.60 In quantitation the data analysis examines the statistics of the standard curve and algorithm of the curve fit. The curve statistics function is accessed by pressing the key below the S icon in the quantitation curve menu. The algorithm of the curve fit can be changed by pressing the key below the curve fit icon. For more information regarding curve fit and statistics refer to section 8.
SECTION 9 – Kinetics The kinetics measurement mode enables the absorbance or % transmittance of an active molecule to be measured over a period of time; for example enzyme analysis of horseradish peroxidase. The absorbance or % transmittance is measured at regular time intervals at a set wavelength over a period of time. The results are plotted on a graph to show the change in absorbance or % transmittance over time.
9.2 METHOD SET UP In this measurement mode all the method set up parameters are accessed through the settings menu. To open the settings menu press the key adjacent to the settings icon in the expanded operating menu. 3.000 ABS -5.000 0.00 5.0 s 10.00 09:02 9.2.1 Kinetics Settings The settings menu enables the wavelength, units, resolution, graph y-axis, operating mode, standard, factor, measurement time, lag time and start on level to be set.
- + 0 .5 2 .5 0 0 0 0 Use the keys at the bottom of the screen to select the digit to be changed and use the keys adjacent to the arrow icons to increase or decrease the number. To change the sign press the key below the + or - icon. Repeat presses will cycle between + and –. Once the required absorbance or % transmittance value has been entered press the key adjacent to the tick icon.
9.2.1.4 Changing the Resolution The resolution of the concentration can be selected from 1, 0.1, 0.01 or 0.001 by repeat presses of the key below the resolution icon. 9.2.1.5 Selecting Concentration Units The concentration units can be selected from a number of options: no units, %, ppm, EBC, SRM, mEq/l, mEq, M, mM, µM, nM, U, U/l, U/ml, g/l, mg/l, µg/l, ng/l, g/dl, mg/dl, µg/dl, mg/ml, µg/ml, ng/ml, µg/µl, ng/µl, mol/l, mmol/l.
9.2.1.8 Selecting a Wavelength 0 4 0 To adjust the wavelength press the key adjacent to the wavelength icon. This will open the number entry screen. Use the keys at the bottom of the screen to select the digit to be changed and the keys adjacent to the arrow icons to increase or decrease the number. Once the required wavelength has been entered press the key adjacent to the tick icon to save the changes and return to the settings menu screen. 0 9.2.1.9 Setting the Kinetics Measurement Time 0 9.
2.500 ABS -0.500 350 450nm 550 If a start on level has been set the Abs/%T icon is displayed in the centre of the screen. The instrument will take photometric readings until the start on level is reached. Once the absorbance/% transmittance level has been reached the readings will be plotted on the graph. 09:02 Once the measurement has commenced it can be aborted by pressing the key below the kinetics measurement in progress icon.
3.000 ABS ppm 0.000 1.000 0.152 0.152 -5.000 0.00 5.0 s 10.00 3.000 ABS ppm 0.000 1.000 0.152 0.152 -5.000 0.00 5.0 s 10.00 In the kinetics moving line screen the initial and final time lines appear as two vertical lines. The line which is selected is represented by a dashed line ---- and can be moved using the keys below the greater than (>) or less than (<) icons at the bottom of the screen. Move the dashed line to the point on the x-axis which is to be the initial time.
SECTION 10 – Saving, Printing and Autologging The utility toolbar in the expanded operating menu provides access to printing, print setup options, opening, saving and deleting results and methods and autologging options. If the method lock has been activated the method selection menu is disabled. Print/print settings Results selection menu 0.000 0.000 Method selection menu Autolog menu ppm ABS 400 nm 0 9 : 02 Fig 10.1 - Expanded Operating Menu 10.
10.1.2 SAVING METHODS TO USB MEMORY STICK 01 TEST 05 02 06 03 07 04 08 In the expanded operating menu press the key adjacent to the method icon to open the method selection menu. Select the method to save to USB memory stick by pressing the key adjacent to the method location. Use the keys below the arrow icons to move page up or down. Hold the key below the create method icon for 2 seconds to open the save to UBS memory stick menu.
10.2.2 OPENING METHODS FROM USB MEMORY STICK 01 TEST 05 02 06 03 07 04 08 In the expanded operating menu press the key adjacent to the method icon to open the method selection menu and use the keys below the arrow icons to move page up or down. Select an empty location to save the method by pressing the key adjacent to the location. Hold the key below the open method icon for 2 seconds. Either a single method or all 48 methods can be opened from the USB memory stick.
1.000 0.099 ppm ABS In the expanded operating menu press the key adjacent to the USB memory stick icon to open the results selection menu. 400 nm 09:02 1 / 1 JW0000~0 A B C D E F G H I J K L M N O P Q R S T U V WX Y Z _ If the USB memory stick doesn’t have any results stored on it only the save to USB memory stick icon will be displayed. To save a result press the key below the save to USB memory stick icon. This will open the results naming menu. The default result name will be displayed.
Operating mode Photometrics Operator Id 2 Method Id 0 Wavelength 420 Sample interval 1 Sample Count 0 Results Destination Internal Cal Time/Date 10 : 54 : 33 05/07/2010 ID TimeDateCell 001 01. 4.0. 091 10 : 54 : 40 05/07/2010 The information displayed is specific to the measurement mode which the result was generated and saved in. Use the keys below the arrow icons to display more information. When opening a spectrum or kinetics result the result can be viewed graphically on an axis or as a text file.
10.7.1 Print Setup 0.000 0.000 To open the print setup menu hold the key adjacent to the printer icon for 2 seconds in the expanded operating menu. ppm ABS 400 nm 09:02 To select the language for the printouts press the key adjacent to English icon. Repeat pressing of the key cycles the language between English, Français, Deutsche, Espânôl and Italiano. English The destination of the printouts can be the internal printer or an external serial printer.
Repeat presses will cycle between 0, 1, 2, 5, 10 or 50. Once the required options have been selected press the key adjacent to the tick icon to save and exit print-set up. 10.7.1.3 Print Setup – QUANTITATION In the quantitation measurement mode print setup menu it is possible to print off the statistics for the standard curve. To select the curve statistics press the key adjacent to the curve statistics icon. Repeat presses of the key will cycle between a tick and a cross icon for selected or deselected.
10.8 AUTOLOGGING 0.000 0.000 ppm ABS 400 nm 0 9 : 02 Expanded Operating Menu 10.8.1 The autolog function enables repeat measurements of the same sample to be performed with a set time period between each measurement. This produces a batch of results for the same sample. The autolog function also enables the results to be autologged to different destinations. The autolog menu is accessed from the utility toolbar in the expanded operating menu by pressing the key adjacent to the autolog icon.
0.000 100.0 ABS %T 400 nm 0002x 003s 09:02 The number of repetitions and time interval will be displayed below the autolog icon. To commence autologging press the key below the measure sample icon. Once the first measurement has been performed the time period starts counting down until it reaches zero and then the next measurement will be taken. This will reduce the repetition number by one. When the number of repetitions reaches zero autologging is complete.
SECTION 11 – Accessories and Spare Parts 11.1 OPTIONAL ACCESSORIES 11.
Squeeze the grey plastic clips together so that the printer top opens. Slot the printer into the top of the instrument and push down until it fits flush to all four sides. Insert the paper roll into the printer – ensuring that there is some paper sticking out of the printer before clicking the grey plastic back into place. Switch the instrument on. The power and error lights on the printer will flash.
11.2.3.1 Automatic 8 cell turret Take the 8 cell turret base plate. Connect the power supply in the bottom of the sample chamber to the connector on the underside of the baseplate. Place the base plate in the sample chamber. Replace screws 1 to 4. Take the 8 cell carousel and place on top of the motor, taking care to align the three ball bearings with the grooves on the motor shaft. Gently push the carousel down onto the motor shaft until it is located into place.
11.2.3.3 Sipper pump 6. For this accessory as well as removing the passive accessory base plate, the front panel of the instrument must also be removed. Loosen screws 5 and 6 until the front panel can be lifted out in a forward direction. 5. Take the sipper base plate. Connect the power supply in the bottom of the sample chamber to the connector on the underside of the base plate. Place the base plate in the sample chamber. Replace screws 1 to 4.
7. Cut a small length of the sipper pump tube and push this over one end of the capillary tube. Connect this to the inlet port of the flow-through cuvette. 8. Route the tube into the two retaining clips located on the base plate at the side of the pump head. 9. Fit the sipper probe and secure using the thumbscrew. Feed the capillary tubing through the tube and up through the sipper probe, allowing sufficient length for it to pass into a suitable receptacle. For pumping: 7. 1. 3. 4. 1.
11.3 USING THE ACCESSORIES 11.3.1 Automatic 8 cell turret 0.000 0.000 When the automatic 8 cell turret is in use the 8 cell turret icon is displayed in the bottom right hand corner of the screen. The current cell position is displayed adjacent to the 8 cell turret icon. The 0 position should always be used for the zero calibration sample. ppm ABS 500 nm 0 09:02 To perform measurements using the automatic 8 cell turret, insert the cuvettes containing the samples into turret positions 1 to 7.
11.3.2 Peltier 0.000 100.0 ABS %T 400 nm 19.9 -> 120.5 09:02 ºC 2 0 . 0 This opens the peltier settings screen. Use the keys at the bottom of the screen to select the digit to be changed and use the keys adjacent to the arrow icons to increase or decrease the number. The temperature can be set in °C or °F by pressing the key adjacent to the °C icon. Repeat presses will cycle between °C and °F.
0.000 100.0 ABS %T 400 nm Confirmation will be needed to start the sipper pump. Press the key adjacent to the tick icon to confirm and start the sipper pump. Press the key adjacent to the cross icon to cancel and return to the expanded operating menu. 09:02 0.000 100.0 ABS %T To stop the sipper pump press the key adjacent to the stop icon. Ensure that the flow through cuvette contains enough sample before pressing the key below the measure sample icon. 400 nm 09:02 11.3.3.
To fine tune the amount of sample uptake press the key below the plus or minus icon to increase or decrease the amount of sample taken up. The recorded time will be adjusted accordingly. Once the fine tuning is complete, or if none is required, press the key adjacent to the tick icon to move to the next stage of the calibration sequence. 000 This stage allows an air gap to be added to the calibration sequence. If an air gap is not required press the key below the 000 icon to set the air gap to zero.
0.000 100.0 To perform a measurement place the sipper tubing into the sample and press the key below the sipper pump icon. ABS %T 400 nm 09:02 0.000 100.0 Confirmation will be needed to start the sipper pump. Press the key adjacent to the cross icon to cancel and return to the expanded operating menu. Press the key adjacent to the tick icon to confirm and start the sipper pump. The pump will run for the previously recorded sample take up time.
The pump direction is displayed by an arrow icon below the sipper peltier icon. The combined sipper peltier pump combines the functionality of the peltier and sipper pump. To open the sipper peltier settings hold the key below the sipper peltier icon for 2 seconds. The settings menu is the same as the sipper pump settings except for the peltier icon in the top left hand corner. Pressing the key adjacent to the peltier icon will open the peltier settings enabling the temperature to be set.
SECTION 12 – Maintenance and Service 12.1 ROUTINE MAINTENANCE Ensure the external surfaces of the unit are clean and free from dust. The sample area should always be kept clean and any accidental spillage should be wiped away immediately. To give added protection when not in use, the unit should be disconnected from the mains supply and covered with the optional dust cover. The only routine maintenance which maybe required is the replacement of the light source.
12.3 SERVICE Our dedicated service staff are on hand to help in the unlikely event that your Jenway equipment develops a fault. Please contact them by one of the following means with a clear description of the problem: E-mail: service@bibby-scientific.com Tel: +44 (0) 1785 810475 Fax: +44 (0) 1785 810471 On occasion it may be necessary for your equipment to be sent back to our Service Department for repair.
SECTION 13 – Troubleshooting 13.1 ERROR CODES If an error code is displayed it will be accompanied by a spanner icon and a symbol to indicate if the error is a warning (caution icon) or fatal (stop icon). If the error is fatal contact your local distributor or Jenway service department (refer to section 12.3). If the error is a warning it may be possible to retry the test. In this case a back icon will also be displayed.
Err 5 Light Saturation Not Found This error indicates that the peak light hasn’t been found at zero. The most likely causes of this error are: Fatal 1. Lamp failure. 2. Deterioating lamp signal. 3. Sample or cuvette in the sample holder. Solution: Ensure that the sample holder is empty. Restart the unit, if the problem persists contact a service technican.
13.2 TROUBLESHOOTING GUIDE Issue Solution Unable to achieve zero absorbance or 100% transmittance when calibrating Ensure that there is not a sample in the sample chamber. Ensure the instrument lid is closed before and during the calibration. Ensure the lamp is working – if the lamp has failed replace the lamp (7310) or lamp module (7315). Unable to achieve a reading when measuring a sample Ensure the correct cuvette is being used so that light isn’t being absorbed by the cuvette.
SECTION 14 – Declaration of Conformity 76
SECTION 14 – Declaration of Conformity 77
SECTION 15 – Glossary of Icons Mode Description Common Back key Common Tick icon - Done/yes Common Cross icon – Cancel/no Common JW icon - Opens expanded operating menu Common Printer icon - Print/open printer settings Common No results to send to printer Common Computer icon - RS232 serial port for connection to an external serial printer or a computer Common No results to send to RS232 Common 78 Icon English English icon - Language selection Common USB me
Common Calibrate to zero icon Common Pencil icon - Select letter/number Common Eraser icon - Delete letter/number Common AB icon - Change letter case/number Common Delete Common Method icon – opens method selection screen Common Create method Common Open method Common - - - - Units icon – opens unit selection screen Resolution Common Common 400nm Wavelength Common ABS/%T Abs/%T icon - Operating mode either absorbance or % transmittance Common Lamp cold
Main Menu Main Menu 80 Instrument settings 12.
Instrument Settings Diagnostics Photometrics Measure sample Photometrics Toggle icon – switches between ABS/%T Concentration Settings menu Concentration Measure to a factor Concentration Measure to a standard Concentration 0.000 ABS Calibrate to zero absorbance Concentration 0.
Spectrum Single less than icon - decrease the wavelength by 1 times scan interval Spectrum Double greater than icon - increase the wavelength by 10 times scan interval Spectrum Single greater than icon - increase the wavelength by 1 times scan interval Spectrum Wavelength icon - manually enter the wavelength Spectrum Add points to the spectral points analysis table Spectrum View spectral points analysis table Spectrum Data point interval – used in print setup Quantita
Kinetics Start kinetics measurement Kinetics Kinetics measurement in progress Kinetics Sigma icon - Post measurement statistics Kinetics Kinetics moving line Kinetics Update factor in settings Kinetics Toggle icon – switches between the initial and final point lines Kinetics Double less than icon - Decrease the time by 5 seconds intervals Kinetics Single less than icon - Decrease the time by 1 second intervals Kinetics Double greater than icon - Increase the time by 5 seco
Accessories Accessories 84 Skip take up time – uses the previously set take up time 000 000 – sets air gap to zero Accessories Stop sipper pump Accessories Reduce sample uptake/reduce air gap Accessories Increase sample uptake/increase air gap Accessories No sipper - Method created on a unit with a sipper accessory fitted Accessories Sipper peltier pump in use Accessories No sipper peltier - Method created on a unit with a sipper peltier accessory fitted Accessories
INDEX ACCESSORIES Accessories - connecting Accessories - using AUTOLOGGING CONCENTRATION CALIBRATION METHOD SETUP MODE SPECIFIC PARAMETERS SAMPLE MEASUREMENT Connecting to a PC Controls Display Keypad Rear panel Declaration of conformity Error codes Good practice guidelines Icon GLOSSARY Installation Unpacking Instrument setup Contrast Expanded operating menu GLP settings Lamp save Mode selection Navigating Utility toolbar KINETICS CALIBRATION
Routine maintenance Safety SAVING, PRINTING AND AUTOLOGGING DELETING METHODS DELETING RESULTS OPENING METHODS OPENING RESU LTS PRINTING RESULTS SAVING METHODS SAVING RESULTS Screen setup SERVICE Spare parts SPECTRUM CALIBRATION DATA ANALYSIS METHOD SETUP MODE SPECIFIC PARAMETERS SAMPLE MEASUREMENT Technical specification Technical support Theory and practice of spectroscopy measurements Spectroscopy measurement Theory of spectroscopy measurements Troublesh
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