KDS Legato 200 Series USER’S MANUAL KDS LEGATO 200 SERIES w w w. k d s c i e n t i f i c .
Table of Contents 2 KDS Legato 200 Series User’s Manual Table of Contents SUBJECT PAGE NO. General Information..................................................................................................6 Safety Information ............................................................................................................................6 Warranty and Repair Information ..................................................................................................7 Manual Description ..
Table of Contents 3 KDS Legato 200 Series User’s Manual SUBJECT PAGE NO. Configuring the Pump’s General Parameters ..............................................................................20 Setting the Time and Date ....................................................................................................21 Setting the Audible Alarms ..................................................................................................22 For Near End of Run Selection .........................
Table of Contents 4 KDS Legato 200 Series User’s Manual SUBJECT PAGE NO. Incorporating Advanced Options ................................................................................................46 Go To Step ............................................................................................................................46 Delay ......................................................................................................................................47 Repeat Step.............
Table of Contents 5 KDS Legato 200 Series User’s Manual SUBJECT PAGE NO. Appendices ..............................................................................................................68 Appendix A: Syringe Volume/Diameter Reference Table..........................................................68 Appendix B: Min/Max Flow Rates ..............................................................................................69 Appendix C: Rack Accessories/Options ..................................
General Information 6 KDS Legato 200 Series User’s Manual General Information SAFETY INFORMATION Please read the following safety precautions to ensure proper use of your syringe pump. If the equipment is used in a manner not specified, the protection provided by the equipment may be impaired. To Prevent Hazard or Injury: USE PROPER LINE CORD Use only the specified line cord for this product and make sure line cord is certified for country of use.
General Information 7 KDS Legato 200 Series User’s Manual WARRANTY AND REPAIR INFORMATION ! CAUTION: REFER TO SAFETY INFORMATION AND SETTING UP THE KDS Legato 200 Series BEFORE PLUGGING IN THE PUMP. Manual Description This manual is designed to provide all operational and program information required to operate and maintain the KDS Legato 200 Series pumps. The functions and features are described in the Technical Specifications section.
General Information 8 KDS Legato 200 Series User’s Manual SPECIFICATIONS Accuracy ± 0.35% Reproducibility ± 0.05% Syringes (Min./Max.) 0.5 µl / 140 ml Flow Rate: Minimum (0.5 µl syringe) Maximum (140 ml syringe) 3.06000 pl/min 216.005 ml/min Display 4.
General Information 9 KDS Legato 200 Series User’s Manual SPECIFICATIONS Atmospheric Specifications: Operating Temperature Storage Temperature Operating Humidity Storage Humidity 4˚C to 40˚C (40˚F to 104˚F) -10˚C to 70˚C (14˚F to 158˚F) See Chart Below 20% to 80% RH, non condensing Mode of Operation Continuous Classification Class I Pollution Degree 1 Installation Category II Supplier Name KDScientific Supplier Address 84 October Hill Rd., Holliston, MA 01746 Supplier Phone Number 508.429.
Product Overview 10 KDS Legato 200 Series User’s Manual Product Overview The KD Scientific Legato 200 Series is the next generation of syringe pumps. The series offers unparalleled ease of use through the high resolution color touch screen user interface. The full touch screen interface enables the user to quickly create configurations and recall them for easy use. The TFT color display presents all the pump operating parameters on an easy to view run screen.
Product Overview 11 KDS Legato 200 Series User’s Manual Easy external connections to a computer or other control devices are made through USB interface or RS232 (9 pin Dsub). Simple ASCII commands make communication with the pump easy. For direct control of the pump, the user can use the I/O interface (15pin Dsub). Two trigger outputs and a trigger input in for external events such as a process parameter is met. The footswitch input will allow the control of the pump through an external device.
Product Overview 12 KDS Legato 200 Series User’s Manual LEGATO 200 SERIES TOUCHSCREEN BUTTON REFERENCE The following buttons are part of the KDS Legato 200 Series software: Settings: Access the settings menu used to adjust the following parameters: Date and Time, Audible Alarms, Pusher Block Force, Display Brightness, RS-232 Communications, etc. Page Down: Used to page down in a display list. Page Up: Used to page up in a display list.
Product Overview 13 KDS Legato 200 Series User’s Manual Selecting An Item From a Menu List Selecting items is easy with the Legato 200 Series. Touch the item to select/highlight the item, then touch again to select it or press Canceling a Selection Canceling selections is easy. Press Messages There are different types of messages. Error messages are shown in yellow. • • • • Critical Run--mode Program Set-up Notices, alarms, confirming, and verified messages are show in green.
Product Overview 14 KDS Legato 200 Series User’s Manual OPERATING INSTRUCTIONS Pusher Block Half nut Release Knobs Syringe Barrel Clamp Guide Rods Syringe Holder Bracket Clamp Knobs Run / Fault LED Lead Screw User Interface Touchscreen Protective Antiglare RS232 Serial Input Analog In (Option) Fuse Holder Main Power Switch *Power Source When powering a pump in a chain through an RS485 cable, toggle switch to RS-485.
Product Overview 15 KDS Legato 200 Series User’s Manual SYRINGE LOADING 1 2 4 5 6 3 10 7 8 9 The Standard Legato 200 Series can accommodate up to two syringes. Any type of syringe, glass, plastic or stainless steel can be used. The pump can accommodate from 0.5 ul to 140 ml syringes. The syringe barrel clamp should be flipped for syringes larger than 30 ml. For syringes smaller than 30 ml, the syringe clamp should be used as shown. 1.
Configuring the KDS Legato 200 Series 16 16 KDS Legato 200 Series User’s Manual Configuring the KDS Legato 200 Series The KDS Legato 200 Series program is easy to set up. Select Mode Select Syringe Manufacturer Size The Legato 200 Series is easily configured. When the unit is powered up, the Configuration Screen will be displayed.
Configuring the KDS Legato 200 Series 17 KDS Legato 200 Series User’s Manual Syringe Selection 1. In the Configuration Display select the syringe button. 2. Select the manufacturer type. Then the available syringe sizes will be displayed. In this example we have selected a KDS 10ml Glass Syringe. Press Press 3. For a Custom Syringe use this selection if syringe manufacturer is not listed.
Configuring the KDS Legato 200 Series 18 KDS Legato 200 Series User’s Manual Flow Rate Selection 1. In the Configuration Display enter the flow rate(s). In this example we want to enter 36.003 ml/min. Press 2. Depending on the Mode, the appropriate set rate screen will be displayed. To change the units, press the units. Press to change the units Enter the flow rate Press to select the maximum volume for the syringe size selected Press to select the minimum volume for the syringe size selected 3.
Configuring the KDS Legato 200 Series 19 KDS Legato 200 Series User’s Manual Target Volume/Time Selection 1. In the Configuration Display enter the target volume or time. In this example we want a volume of 5 ml. ✐ NOTE: Target time is only allowed in infusion only or withdrawal only modes. 2. Depending on the Mode either Target Volume (I/W or W/I) will appear or Target Volume or Target Time Selection (I only or W only). Press Here ✐ NOTE: For time enter h:m:s. or press again to select secs.
Configuring the KDS Legato 200 Series 20 KDS Legato 200 Series User’s Manual Previewing the Configuration It is simple to view the Pump Configuration on the RUN display. Press to view Pump Configuration After you have verified the configuration. Press to run the displayed configuration. Press to preview configuration Configuring the Pump’s General Parameters The pump has basic parameters that should be configured during initial start-up.
Configuring the KDS Legato 200 Series 21 KDS Legato 200 Series User’s Manual Setting the Time and Date The date and time is displayed on each screen. A built in battery backup will maintain the date and time even if the pump loses power or is unplugged for a period of time. In the Set-up screen, press Date/Time ✐ ✐ Press NOTE: The date AND time must be entered. NOTE: Remember to activate the battery by removing the plastic tab on the bottom of the pump.
Configuring the KDS Legato 200 Series 22 KDS Legato 200 Series User’s Manual Setting the Audible Alarms In the Set-up screen , press Audible Alarms. Select the alarms, a check mark will indicate the alarms selected. Press If these alarms are selected the pump will provide an audible alarm. Accept the changes made by pressing and go back to the Set-up screen. Cancel the changes made by pressing and go back to the Set-up screen.
Configuring the KDS Legato 200 Series 23 KDS Legato 200 Series User’s Manual For Near End of Run Selection Select the % volume dispensed. For example, if you select 80%, the pump will sound an alarm (3 short beeps) when the 80% of the volume is dispensed. Accept the changes made by pressing and go back to the Set-up screen. Cancel the changes made by pressing and go back to the Set-up screen. Press to select % volume dispensed For a Custom % Use this selection if the % is not listed.
Configuring the KDS Legato 200 Series 24 KDS Legato 200 Series User’s Manual Password Protection A password can be enabled to prevent accidental changes to program settings. Unlocking the touchscreen requires a password, defined by the user. Set password Setting the Password To set the Password go to the Settings screen. Press the Set Password button. Enter your desired password, up to 8 digits, and press the “ACCEPT” button.
Configuring the KDS Legato 200 Series 25 KDS Legato 200 Series User’s Manual Setting the Force Limits The maximum linear force of the syringe pump is 75 lbs or 35 kgs. This force is sufficient to damage delicate, low volume syringes, as well as many standard syringes if pumping viscous fluids. The % of maximum force applied by the pusher block can be changed. In the set up screen, press Force. ✐ NOTE: A minimum value of approximately 30% is required to overcome friction forces in the mechanism.
Configuring the KDS Legato 200 Series 26 KDS Legato 200 Series User’s Manual Setting Display Brightness The backlight can be adjusted. In the Set-up screen, select Display Brightness Press to select % Display Brightness Select the % Display Brightness. For example, if you select 80%, the brightness of the display will be 80% of the maximum. Accept the changes made by pressing and go back to the Set-up screen. Press Here Cancel the changes made by pressing and go back to the Set-up screen.
Configuring the KDS Legato 200 Series 27 KDS Legato 200 Series User’s Manual Setting the Pump Address Up to 100 pumps can be linked together. Each pump can be given a unique address. In the Set-up Screen press Pump Address. Valid entries are 00 to 99. Enter the pump address. Accept the changes made by pressing and go back to the Set-up screen. Cancel the changes made by pressing and go back to the Set-up screen.
Configuring the KDS Legato 200 Series 28 KDS Legato 200 Series User’s Manual Resume Run on Power Loss If Resume Run on Power Loss is selected the pump will power up running when power resumes. ✐ NOTE: This feature is only valid in Infuse Only or Withdraw Only modes. This feature will only execute if the pump is running at the time when power is lost, and was running with no target set.
Operating the KDS Legato 200 Series 29 KDS Legato 200 Series User’s Manual Operating the Pump The Legato 200 Series is easy to operate because all the pump parameters are shown on the RUN Screen. Depending on the mode of the pump, different parameters will be shown on the display. The Legato 200 Series Run Screen is shown below.
Operating the KDS Legato 200 Series 30 KDS Legato 200 Series User’s Manual Running the Pump Simply press the button to start the pump. When the pump is running the is shown. Press the button button to pause the program. The fast forward and fast reverse key are not active when the pump is running. The Legato 200 Series can also be run in a veritical position. All screens will rotate automatically when the pump is placed on its side. 508.429.6809 fax 508.893.0160 e-mail info@kdscientific.
Operating the KDS Legato 200 Series 31 KDS Legato 200 Series User’s Manual Custom Program Workflow The following flow diagram describes the use of an Custom Program: Select Program Select Syringe Manufacturer & Size OR Create New Syringe Type Review Program Steps (Pump Operation Profiles) Enter Pump Operation Profile Parameters Validate Commands and I/O Operations The software provides the user with a variety of prompts and warnings to help guide the setup of programs.
Operating the KDS Legato 200 Series 32 KDS Legato 200 Series User’s Manual STEP-BY-STEP OPERATION EXAMPLE: USING A CUSTOM PROGRAM The following steps describe operation using a Custom Program. In the following description, we are assuming the use of a Program that is already saved on the KDS Legato 200 Series.
Operating the KDS Legato 200 Series 33 KDS Legato 200 Series User’s Manual Configuring Custom Programs Press to see the pump information screen The Legato 200 Series Programmable Models offer the flexibility of configuring custom programs including multistep programs. The programs and steps can also be triggered to start and stop with events and external I/O, and user defined inputs. The maximum number of steps is 800 for all the programs.
Operating the KDS Legato 200 Series 34 KDS Legato 200 Series User’s Manual Configuring Custom Programs The first thing to do on a custom program is to name the program.
Operating the KDS Legato 200 Series 35 KDS Legato 200 Series User’s Manual Setting A Time Delay You can set the KDS Legato 200 Series to run at a specific date and time in the future. This delayed run function allows you to set up all the parameters for operation, then have the pump start unattended at the required time. SETTING A TIME DELAY FOR PROGRAM OPERATION: 1. From the Program Configuration screen, choose the Set Delay Parameters button to enter the Set Delay Date and Time screen. 2.
Operating the KDS Legato 200 Series 36 KDS Legato 200 Series User’s Manual Creating a Custom New Program If none of the Programs stored on the KDS Legato 200 Series match your requirements, you can create a new Program and store the Program for use. The following flow diagram outlines the basic steps you will follow to create a new Program: New User-Defined Program creation workflow TO CREATE A NEW USER-DEFINED PROGRAM 1.
Operating the KDS Legato 200 Series 37 KDS Legato 200 Series User’s Manual TO CREATE A NEW USER-DEFINED PROGRAM (CONTINUED) 4. When you have entered the desired name for your Program, choose the Accept button to go to the Program Configuration screen. Your new Program will now be listed in the Program Select button. 5. Choose the Syringe Select button and complete the description of the syringe by following the procedure outlined previously in the Syringe Selection section of this Guide. 6.
Operating the KDS Legato 200 Series 38 KDS Legato 200 Series User’s Manual Modifying an Existing User-Defined Program Once you have created a Program or have reviewed an Existing Program, you may have a need to modify the structure of the Program (i.e. the types of Steps and Commands included, not just changes in the operating parameters for a specific Step). The following procedures will help you to efficiently modify a stored Program. CHANGING THE ORDER OF STEPS IN A PROGRAM 1.
Operating the KDS Legato 200 Series 39 KDS Legato 200 Series User’s Manual PUMP OPERATION PROFILES The KDS Legato 200 Series Existing Programs and all UserDefined Programs use Pump Operation Profiles as part of the Programs controlling the pump. These profiles are combined with a syringe selection and any Commands or Input/Output triggers to create a complete Program. The following sections describe each profile along with instructions for setting up the profile.
Operating the KDS Legato 200 Series 40 KDS Legato 200 Series User’s Manual Constant Rate Profile TO SET UP/EDIT A CONSTANT RATE PROFILE: The Constant Rate profile is used to run the pump at a defined flow rate and for a specific pumping mode (infuse or withdraw) up to a target volume or for a defined time period. 1. From the Step Definition screen for a Constant Rate profile, choose the Direction button to toggle the Flow Mode between Infuse and Withdraw operation. 2.
Operating the KDS Legato 200 Series 41 KDS Legato 200 Series User’s Manual Ramp Profile 2. Choose the Set Start & End Rate button to enter the Set Start & End Rates screen. The Ramp Rate profile is used to create a linearly increasing or decreasing flow rate for a single pumping mode (infuse or withdraw) over the course of a defined time period.
Operating the KDS Legato 200 Series 42 KDS Legato 200 Series User’s Manual Stepped Profile The Stepped Rate profile is used to create an increasing or decreasing flow rate that varies in a series of equal steps between a starting and ending flow rate.
Operating the KDS Legato 200 Series 43 KDS Legato 200 Series User’s Manual TO SET UP/EDIT A STEPPED RATE PROFILE: 1. From the Stepped Definition screen for a Stepped Rate profile, choose the Direction button to toggle the Flow Mode between Infuse and Withdraw operation. 9. Select the Total Time button (it should be selected by default as indicated by the active data entry box to the right of the button). Enter the desired value using the keypad. 10.
Operating the KDS Legato 200 Series 44 KDS Legato 200 Series User’s Manual Pulse Profile The Pulse Rate profile is used to create an oscillating infusion or withdrawal profile that varies between a starting flow rate and a second rate that can be either or higher or lower than the start rate. The flow rate is constant within each segment of the pulsed flow profile and the user defines the total number of repetitions (pulses) for the oscillating pattern.
Operating the KDS Legato 200 Series 45 KDS Legato 200 Series User’s Manual TO SET UP/EDIT A PULSE RATE PROFILE: 1. From the Pulse Definition screen for a Pulse Rate profile, choose the Direction button to toggle the Flow Mode between Infuse and Withdraw operation. Set Pulse Times screen used to define the length of time the pump will operate at each pulse step level Step Definition screen for a Pulse Profile with Volume mode selected 2. Choose the Rates button to enter the Set Pulse Rates screen. 3.
Operating the KDS Legato 200 Series 46 KDS Legato 200 Series User’s Manual Incorporating Advanced Options (Commands and I/O) Into A Program KDS Legato 200 Series Programs can incorporate a variety of commands that expand the versatility of the system by permitting convenient looping, chaining of events, sending signals to external devices, or accepting inbound signals from external devices. These Advanced Options controls are described in this section of the Guide.
Operating the KDS Legato 200 Series 47 KDS Legato 200 Series User’s Manual Repeat Step Delay The Delay Command is used to insert a time delay in a procedure. You can program a time delay between 0.2 seconds up to 99:99:99 (99 hours, 99 minutes, 99 seconds). TO INSERT A TIME DELAY IN A PROGRAM: 1. From the Program Main screen, choose Step Definition to enter the Step Definition screen. 2. Highlight (select once) the Program Step that you want the time delay to follow. 3.
Operating the KDS Legato 200 Series 48 KDS Legato 200 Series User’s Manual Link Program Stop The Link Command is used to insert a link to another stored Program into the operating sequence of a Program. You can Link a Program any place in the Program sequence. The Stop Command is used to instruct the pump to stop operation. This Command can be used in conjunction with I/O Events to control pump operation based on external events. TO INSERT A STOP COMMAND IN A PROGRAM: 1.
Operating the KDS Legato 200 Series 49 KDS Legato 200 Series User’s Manual I/O EVENT TRIGGERS The KDS Legato 200 Series pump permits easy connection to external I/O devices via a variety of interfaces, including a built-in 15-pin D-sub connector. The KDS Legato 200 Series software incorporates simple commands for incorporating I/O controls into your pump Programs. Integrated commands for control of an external valve, multi-pump synchronization, and general trigger signals are integrated in the software.
Operating the KDS Legato 200 Series 50 KDS Legato 200 Series User’s Manual I/O (OUT) Trigger The I/O (OUT) Trigger command is used to send a signal to an external device when the Step in the Program is reached. TO INSERT AN I/O (OUT) TRIGGER COMMAND: 1. From the Program Main screen, choose Step Definition to enter the Step Definition screen. 2. Highlight (select once) the Program Step that you want the I/O (OUT) Trigger to follow. 3. Choose the ‘INS STEP’ button to access the Program Steps Menu screen.
Operating the KDS Legato 200 Series 51 KDS Legato 200 Series User’s Manual I/O (IN) Event The I/O (IN) Event command is used to have the pump pause and wait for a signal from an external device or for a manual user input at the touchscreen. These external signals are communicated via Pin 2 on the 15-pin I/O Connector port. TO INSERT AN I/O (IN) EVENT COMMAND: 1. From the Program Main screen, choose Step Definition to enter the Step Definition screen. 2.
Operating the KDS Legato 200 Series 52 KDS Legato 200 Series User’s Manual I/O (IN) Event Link The I/O (IN) Event Link command is used to have the pump jump to a linked program if the signal is received. The external signals are communicated via Pin 2 on the 15-pin I/O Connector. TO INSERT AN I/O (IN) EVENT LINK COMMAND: 1. From the Program Main screen, choose Step Definition to enter the Step Definition screen. 2.
Operating the KDS Legato 200 Series 53 KDS Legato 200 Series User’s Manual MANAGING LEGATO 200 SERIES PROGRAMS All Existing and User-Defined Programs are stored in the Program List for ongoing use. Over time, you may want to manage previously created Programs to keep your Program List easy to navigate. In addition, the KDS Legato 200 Series allows you to export Program information to an externally connected pump or to a computer that will be used to control operation.
Operating the KDS Legato 200 Series 54 KDS Legato 200 Series User’s Manual Appending A Program Deleting A Program Follow these instructions to Append a previously created Program into a new Program: Follow these instructions to delete a previously saved Program: 1. From the Program Configuration screen, choose the Program Name button to enter the Mode Selection screen. 1. From the Program Configuration screen, choose the Program Name button to enter the Mode Selection screen. 2.
Operating the KDS Legato 200 Series 55 KDS Legato 200 Series User’s Manual LEGATO 200 SERIES PROGRAM EXPORT AND IMPORT TRANSFER A PROGRAM BETWEEN PUMPS The Legato 200 Series pump permits the daisy chaining of multiple pumps, as well as integration with/control by an external computer through HyperTerminal.
Operating the KDS Legato 200 Series 56 KDS Legato 200 Series User’s Manual Exporting A Program To An External Computer Follow these instructions to export a Program to an external computer. 1. Connect the pump to the external computer via a RS-232 or USB cable. IMPORTING A PROGRAM FROM AN EXTERNAL COMPUTER Follow these instructions to import a Program from an external computer. 2. On the computer, open the HyperTerminal application and verify that the computer can communicate with the pump. 1.
Operating the KDS Legato 200 Series 57 KDS Legato 200 Series User’s Manual EXTERNAL PUMP CONTROL 4. Set up the Port Settings as shown below and click OK. This section of the KDS Legato 200 Series manual describes the control of the pump using an external computer device. If communicating via the USB Port, see Appendix D for one time installation instructions of USB Driver.
Operating the KDS Legato 200 Series 58 KDS Legato 200 Series User’s Manual PUMP CHAIN COMMANDS The Pump Chain commands allow all pump control information to be managed from an external computer source. These commands can also be used to control a series of pumps (up to 100) from a single computer interface. KDS Legato 200 Series commands are communicated to the pump via the RS-232 or USB port interfaces through a terminal program such as HyperTerminal.
Operating the KDS Legato 200 Series 59 KDS Legato 200 Series User’s Manual Command errors CATALOG Command errors are displayed when the command is unrecognized, entered in the wrong mode, or the state of the pump keeps the command from executing (i.e. using the IRUN command if a limit switch is active). Displays a catalog of programs stored in the pump.
Operating the KDS Legato 200 Series 60 KDS Legato 200 Series User’s Manual ECHO Sets or displays the RS232/USB echo state. Valid states are on or off. Command format: echo [on|off] Query response: [##:]OFF [##] or: [##:]ON [##] FTSWITCH Sets or displays the footswitch setting. The footswitch may be used in a toggle on or off mode (momentary), a press to run mode (falling), or a release to run mode (rising).
Operating the KDS Legato 200 Series 61 KDS Legato 200 Series User’s Manual TIME Sets or displays the date and time.
Operating the KDS Legato 200 Series 62 KDS Legato 200 Series User’s Manual STOP / STP Stops the pump. Command formats: stop stp WRUN Runs the pump in the withdraw direction. Configuration Run Screen mode only. Command format: wrun IRAMP Sets or displays the infusion rates while ramping. Configuration Run Screen mode only. Command format: iramp [{start rate} {start units} {end rate} {end units} {ramp time in seconds}] Query response: [##:]Ramp not set up.
Operating the KDS Legato 200 Series 63 KDS Legato 200 Series User’s Manual WRATE Sets or displays the withdraw rate. Configuration Run Screen mode only. The rate argument may be replaced by “max” or “min” to set the maximum or minimum rate, respectively. “lim” may be used to display the range limits. IVOLUME Displays the infused volume. Configuration Run Screen mode only.
Operating the KDS Legato 200 Series 64 KDS Legato 200 Series User’s Manual Time commands CITIME Clears the infused time. Configuration Run Screen mode only. Command format: citime CTIME Clears both the infused and withdrawn times. Configuration Run Screen mode only. Command format: ctime CTTIME Clears the target time. Configuration Run Screen mode only. Command format: cttime CWTIME Clears the withdrawn time. Configuration Run Screen mode only. Command format: cwtime ITIME Displays the infused time.
Operating the KDS Legato 200 Series 65 KDS Legato 200 Series User’s Manual Digital I/O commands Internal commands INPUT Reads and displays the trigger input port status. STATUS Displays the raw status for use with a controlling computer. Command format: input Query response: [##:]Low. [##] or: [##:]High. Command format: status The output is in three integer fields and one flag field, all separated by spaces and terminated by a carriage return / linefeed pair.
Operating the KDS Legato 200 Series 66 KDS Legato 200 Series User’s Manual ERROR CODES Program Errors ERROR 301- Delay time out of range, must be at least 0.20 seconds. ERROR 302- Number of steps must be between 2 and 500. ERROR 306- Download to remote pump incomplete. ERROR 307- Syringe size and flow rate not compatible, cannot set motor speed. ERROR 308- File exists. ERROR 309- Linked to program does not exist. ERROR 310- Not all fields have been set. ERROR 311- Rate is out of range.
Operating the KDS Legato 200 Series 67 KDS Legato 200 Series User’s Manual Critcal Errors Confirm ERROR 401- Infuse rate could not be saved to disk. Contact factory. CONFIRM- Press the lock button again, to lock the screen. ERROR 402- Withdraw rate could not be saved to disk. Contact factory. CONFIRM- Press button again to delete this program. CONFIRM- ERROR 403- Program step could not be saved to disk. Contact factory. Press button again to reset calibration date.
Appendices 68 KDS Legato 200 Series User’s Manual Appendices APPENDIX A: SYRINGE VOLUME/DIAMETER REFERENCE TABLE KD Scientific Stainless Steel Size Diameter 2.5 ml 8 20 50 100 4.851 mm 9.525 19.13 28.6 34.9 Air–Tite HSW Norm-Ject Size Diameter 4.69 mm 9.65 12.45 15.9 20.05 22.9 29.2 1. ml 2.5 5 10 20 30 50 Becton Dickinson ‘Plasti-pak’ Size Diameter 1. ml 3 5 10 20 30 50 60 4.699 mm 8.585 11.989 14.427 19.05 21.59 26.594 26.594 Terumo Japan Size Diameter 1. ml tb 1 ml vc 2.5 5 10 20 30 50 4.
Appendices 69 KDS Legato 200 Series User’s Manual APPENDIX B: MIN/MAX FLOW RATES Nominal Minimum/Maximum Flow Rates for Various Syringes. (Actual Limits will vary depending on syringe manufacturer) Syringe Size Syringe ID Minimum Rate Units Maximum Rate Units 0.5 µl 0.103 mm 3.06000 pl/min 1.59133 µl/min 0.206 mm 12.2400 pl/min 6.36532 µl/min 1 µl 0.1457 mm 5 µl 0.343 mm 2 µl 10 µl 25 µl 50 µl 100 µl 250 µl 500 µl 1 ml 1 ml 3 ml 5 ml 0.485 mm 0.729 mm 1.030 mm 1.457 mm 2.304 mm 3.
Appendices 70 KDS Legato 200 Series User’s Manual APPENDIX C: RACK ACCESSORIES/OPTIONS Mounting a 4 X 140 Multi-Rack (Continued on page 69) Pusher Block Assembly 1. Remove Dual Syringe Anti-Syphon Bracket by unscrewing and removing the two T Knobs and two Spring Washers. 2. Attach the Pusher Bar to the Pusher Block using two flat head screws. 3. Attach the Anti-Syphon bar to the Pusher Bar with three Thumbscrews and three Springs.
Appendices 71 KDS Legato 200 Series User’s Manual Mounting a 4 X 140 Multi-Rack (Continued) Syringe Holder Assembly 1. Remove four set screws from the Dual Syringe Holder using the provided Hex Key. 2. Attach the Multi-Holder onto the Dual Syringe Holder using four #10-32 x 1⁄2" screws using a phillips head screwdriver. 3. Attach Clamp Bar by screwing the Multi-Syringe Clamp Knob into the threaded bar attached to the Multi-Holder. 508.429.6809 fax 508.893.0160 e-mail info@kdscientific.com web www.
Appendices 72 KDS Legato 200 Series User’s Manual Mounting a 6/10 Multi-Rack (Continued on page 71) Pusher Block Assembly 1. Remove Dual Syringe Anti-Syphon Bracket by unscrewing and removing the two T Knobs and two Spring Washers. 2. Attach the Pusher Bar to the Pusher Block with the two flat head screws using a phillips head screwdriver. 508.429.6809 fax 508.893.0160 e-mail info@kdscientific.com web www.kdscientific.
Appendices 73 KDS Legato 200 Series User’s Manual Mounting a 6/10 Multi-Rack (Continued) Syringe Holder Assembly 1. Remove Syringe Clamp bracket from the Syringe Holder by unscrewing knob. 2. Remove four set screws from the Dual Syringe holder using the provided Hex Key. 3. Place the Multi-Holder on the Dual Syringe Holder and attach using four #10-32 x 1⁄2" Screws using phillips head screw driver. 4. Screw the two Post Clamp Guides into Multi Syringe Holder 4.
Appendices 74 KDS Legato 200 Series User’s Manual Syringe Loading - 4 x 140 & 6/10 Multi-Racks 1. Release Tabs 2. Syringe Holder 3. Retaining Bracket (Syringe Holder) 4 4. Anti-Syphon Bar 5. Pusher Bar 7 1 6. Clamp Bar 7. T-Knobs 8. Plunger Holder 8 9. Thumbscrew 2 5 9 6 3 1. Raise or remove Clamp Bar (6) by unscrewing thumb screw (9). 2. Loosen T-Knobs (7), located under the Syringe Holder (2), and slide the Retaining Bracket (3) on the Syringe Holder open. 3.
Appendices 75 KDS Legato 200 Series User’s Manual Mounting a Micro Liter Multi-Rack (Continued on page 74) Pusher Block Assembly 1. Remove the Pusher Blocks Anti-Syphon Bracket by unscrewing and removing the T-Knobs (2) and Spring Washers (2). 2. Attach Micro Anti-Syphon Bracket using T-Knobs (2) and Spring Washers (2). 508.429.6809 fax 508.893.0160 e-mail info@kdscientific.com web www.kdscientific.
Appendices 76 KDS Legato 200 Series User’s Manual Mounting a Micro liter Multi-Rack Syringe Holder Assembly 1. Remove Syringe Clamp and knob from the Syringe Holder 2. Remove two set screws from the Dual Syringe Holder with the provided Hex Key. 3. Place the assembled Micro Syringe Holder onto the dual syringe holder and screw in the two Standoff Bars (2). 4. Place the Clamp Bar Assembly onto the Micro Syringe Holder and push and screw the Thumbscrew Caps (2) into the Standoff Bars. 508.429.
Appendices 77 KDS Legato 200 Series User’s Manual Syringe Loading – Micro Liter Multi-Rack 1 2 6 3 4 5 11 7 8 10 9 1. Release Tabs 2. Pusher Block 3. Retaining Bracket (Pusher Block) 4. 5. 6. 7. Bracket Clamping Knob Thumbscrew Cap Screw Cap Retaining Bracket (Syringe Holder) 8. 9. 10. 11. Syringe Holder T-Knob Bumper Clamp Bar Assembly Front view of KDS Legato 200 Series showing controls for micro liter multi-rack syringe loading 1.
Appendices 78 KDS Legato 200 Series User’s Manual Syringe Loading – Push-Pull Mechanism 9 6 7 2 3 5 Locking Pins 8 4 1 1. Thumbscrews 2. Retaining Bracket, barrel flange 3. Retaining Bracket, plunger flange 5. Pusher Block 8. Locking pins 6. Syringe Clamp 9. Stationary Syringe holder 4. Floating Syringe holder 7. Syringe Clamp Knob Installing a Syringe 1. Loosen Thumbscrews (1) to release the Retaining Brackets (2 & 3) located on the Floating Syringe Holder (4) and Pusher Block (5). 2.
Appendices 79 KDS Legato 200 Series User’s Manual APPENDIX D: EXTERNAL CONNECTIONS User I/O Connector Specifications All Inputs are pulled high to +5 Vdc through a 10k resistor All Outputs are at TTL Logic Levels. Pulse duration should be 0.1s minimum. Pin Assignments 1. Direction control input Rising edge sets pump to infuse Falling edge sets pump to refill 2. Trigger Input Event trigger – falling edge triggers a program event 3.
Appendices 80 KDS Legato 200 Series User’s Manual Analog Input Option ! 10V Turning the Potentiometer CW will increase the motor speed (center pin is 10 VDC) and turning the Potentiometer CCW will decrease the motor speed (center pin is 0 VDC). ✐ ✐ NOTE: Although the Analog Mode cycle time is 100 ms, when changing from min to max (or max to min) rates please allow a minimum of 250 ms. ✐ NOTE: Voltage must be set to 0 VDC when pump is turned on.
Appendices 81 KDS Legato 200 Series User’s Manual Utilizing the USB Interface Mating Face Pin # Signal 1 +5V 3 + Data 2 4 - Data GND USB VIRTUAL COMMPORT DRIVER INSTALLATION When you connect the KDS Legato 200 Series pump to a computer via USB for the first time, Windows will seek to install a driver for communication. The following section details the installation of the Virtual CommPort Driver supplied with the KDS Legato 200 Series Pump. 1. Connect the pump to the computer via USB.
Appendices 82 KDS Legato 200 Series User’s Manual USB VIRTUAL COMMPORT DRIVER INSTALLATION (CONTINUED) 3. Select Browse my computer for driver software. 4. Click Browse and navigate to the pump drivers folder located on the CD supplied with the pump. Select the file KDS Scientific USBCDC.inf. Make sure the Include Subfolders box is checked and then click Next. 5. If a warning message regarding the driver publisher is displayed, choose Install this driver software anyway. 6.
Appendices 83 KDS Legato 200 Series User’s Manual RS-232 Specifications Pin # Signal 2 TXD 5 GND 3 1, 4, 6-9 RXD N/C RS-232 9-Pin D-SubMale Baud Rates: 9600 19200 38400 57600 115200 Word size – 8 Parity – None Stop Bits – 2 Pump PC RS-232 Specifications 508.429.6809 fax 508.893.0160 e-mail info@kdscientific.com web www.kdscientific.
Appendices 84 KDS Legato 200 Series User’s Manual RS-485 CAUTION: DO NOT CONNECT TO FIREWIRE PORTS ON A PC. DAMAGE MAY OCCUR TO PUMP AND/OR PC. ! Mating Face Pin # 1 2 RS-485 In Signal PWR IN GND 1 2 3 4 3 RS-485 A 6 5 TIED TO PIN 6 5 4 6 RS-485 Out Signal RS-485 B TIED TO PIN 5 PWR OUT (+30V)* * TIED TO PIN 4** ** GND TIED TO PIN 3** RS-485 A ** Notes Protected by resettable fuse 100 Ω, ½ w to GND termination RS-485 B ** RS-485 In/Out - IEEE-1394 Sockets P.C.
Appendices 85 KDS Legato 200 Series User’s Manual APPENDIX E: MAINTENANCE Maintenance KDS Legato 200 Series pumps require no special maintenance other than keeping them clean by avoiding accidental spills of pumped material. Refer to the chemical compatibility chart below for specific chemical resistance information. To clean the exterior surfaces, use a lint-free cloth to remove loose dust. Use care to avoid scratching the clear display window.
Appendices 86 KDS Legato 200 Series User’s Manual Fuse Replacement Key Information 1. Make sure the power cord is disconnected from the main supply before servicing the fuse. 2. Use only Type 3AG, 1/4" x 11/4, ½ amp, 250 volt; Type T (time delay) fuses (KD Scientific Part No. 5146-037 or equivalent). Turn off power and remove power cord from power module. Use a straight blade screw-driver to pry open the access door.
Appendices 87 KDS Legato 200 Series User’s Manual Upgrading Legato 200 Series Software ✐ NOTE: Prior to upgrading, users will need to install the KDS Legato 200 Series USB driver as well as the bootloader driver file (KDS Legato 200 Series-Bootloader.inf). Reference “USB Virtual Comport Driver Installation”. 1. Upload the latest software version to your desktop (format is filename.srec). 2. Disconnect all I/O devices and then connect the pump to PC using a USB cable. 3.
Appendices 88 KDS Legato 200 Series User’s Manual TROUBLESHOOTING 1. Infusion Accuracy To ensure infusion accuracy always use new syringe(s) and measure syringe bore diameter and enter actual dimensions in millimeters (mm) using the Custom Syringe entry option. Additionally, make sure that the guide rods and lead screw are properly lubricated. Low Battery 2. RS-232 Difficulties Verify that the baud rates, data framing parameters, data bits, parity, and stop bits on all devices are the same.
Appendices 89 KDS Legato 200 Series User’s Manual APPENDIX F: ORDERING INFORMATION Item No. Description Model Versions Item No.
Declaration of Conformity Manufacturer: KD Scientific, Inc. 84 October Hill Road Holliston, Massachusetts 01746-1388, U.S.A. Phone: (508) 429-6809 We herewith declare that the following product: Product Name: Model No.