KERN OCO-2 Operating Manual Inverted System Microscopes
Content 1. Introduction ..................................................................................................................................1 2. Safety Symbols ..............................................................................................................................1 3. Meaning of the text symbols.........................................................................................................2 4. Precautions When Unpacking the Microscope ............................
1. Introduction Dear friend, thank you for using the inverted biological microscope made by our company. It is our great honor to have you as our subscriber. In order to give you timely knowledge of how to use the product, we have especially written this manual. Efforts have been made to ensure thoroughness in contents, and brevity in style. It is our hope that you can get the basics about the product such as its configurations, the ways to operate it, fault clearance, and maintenance.
3. Meaning of the text symbols. Caution： Negligence of the warnings in the manual may cause Physical harm or mechanic damage (the objects nearby may also be affected). ！ Caution： Negligence of the warnings in the manual may cause Physical harm or mechanic damage (the objects nearby may also be affected). A reminding notice (for easy operation and maintenance) 4.
3 Lanp chamber C-mountvideo Camera C-mountdigital camera C-mountdigitalport C-mount video port three-position reflector slider DSZ main body Lambda P la te bulb cover Analyzer teardrop stage inser t plate 60mm Petridish holder v address plain holder PH UCIS objective auxilia ry stage specimen stage re ctangular stage With flexible right handle UCIS objective centering telescope 76x26 haemacyto Meter plate holder Objective DIC polarizer (Diagram Phase slide 5.
. Preparation for use Please refer to diagram 1 on page 3 about the names of the following items. 6-1 Attaching the Eyepieces 6-1-1 Remove the eyepieces' dust caps. 6-1-2 Insert G10X eyepiece into the eyepiece sleeve. 6-2 6-1 Attaching the Objectives 6-2-1 Remove the stage center plate and attach the objectives to the revolving nosepiece through the hole on the stage left by the plate.
6-5 Attaching the Holder for 76mm * 76mm slice 6-5-1 This part is used for the observation of the76mm * 76mm slice. 6-5-2 Open the spring clip of the rectangular stage with flexible right handle, slide the holder into its right position (Be sure that the rectangular groove facing up), loose the clip gently, and finally put the Slice in place. 6-5 6-6 Connecting the Cables 6-6-1Cables and cords are vulnerable when bent or twisted. Never subject them to excessive force.
6-8-2 While holding the mounting lever of the filter holder, insert a filter. (1) Hold the filter by its edge to avoid leaving fingerprints or smudges on the filter surfaces. (2) After the illumination has been ignited, the filter will be very hot. Be sure to set the main switch to "O"(OFF) and allow the filter holder and filters to cool down before replacing filters. 6-8-3 Engage each filter in the light path by moving the filter holder in the direction of the arrow.
7-3-2 For detailed information, please refer to the specifications about patch clamp, thermostatic cover, and the thermostatic stage. 8. Operations 8-1 The procedure of transmitted light bright field observation and phase contrast observation, please refer to diagram 2 in page 08. For more detail information, please refer to 8-2 and 8-3. Transmitted light brightfield observation Set the main switch to "I"(ON) and adjust the intensity of illumination.
8-2 The details of transmitted light brightfield observation procedure 8-2-1 Set the main switch to "I"(ON) and adjust the intensity of illumination. A. Make sure that the light intensity control knob is in the MIN(minimum intensity)position and set the main switch to "I"(ON). B. Rotate the knob toward MAX(maximum intensity)to increase the intensity and the illumination brightness. C. When the microscope system is not be used for a long period,set the main switch to "O"(OFF).
(3) Detaching the Fine Adjustment Knob The fine adjustment knob is designed detachable in order to prevent interference between the knob and the operator's hand manipulating the X- and Y- axis knobs. Loosen the clamping screw using the Allen screwdriver and remove the fine adjustment knob. After detaching, the seat of the fine adjustment knob is hollowed to facilitate manipulation with the thick of a finger.
8-2-12 Insert the filters(Please refer to 6.8) 8-2-13 Adjust the brightness 8-2-14 Start observation 8-3 Observation of phase contrast 8-3-1 Set the main switch to "I"(ON)(Please refer to 8.2.1) 8-3-2 Disengage the filter other than frost filter from the light path. 8-3-3 Select the light path with the light path selector lever. 8-3-4 Attach the phase contrast slider. 8-3-5 Engage the objective matched with the phase contrast slider in the light path.
8-3-10 Place the specimen on the stage. 8-3-11 Bring the specimen into focus. 8-3-12 Adjust the brightness. 8-3-13 Adjust the interpupillary distance and diopter. 8-3-14 Engage the objective to be used in the light path and bring the specimen in focus. 8-3-15 Adjust the aperture iris and field iris diaphragms. 8-3-16 Engage the required filters. 8-3-17 Adjust the brightness. 8-3-18 Start observation.
9. Troubleshooting Guide Under certain conditions, performance of the microscope may be adversely affected by factors other than defects. If problems occur, please review the following list and take remedial action as needed. If you cannot solve the problem after checking the entire list, please contact your local representative for assistance. Problem Cause Remedy Power cord of the power supply unit is unplugged. Plug in the power cord. Main switch of the power supply unit is not ON.
Problem Cause Remedy f ) Visibility of the image is poor: Image is not sharp. Contrast is poor. Details are poorly visible. Poor contrast observation. g) The image is blurred. Objective is engaged Make sure that revolving incorrectly in the light path. nosepiece clicks into place correctly. during Replace the plastic culture vessel with a glass vessel. Specimen is tilted with respect to the stage. h) Field of one eye does not match that of the other.
power cord from the wall outlet, then allow the old bulb and lamp socket to cool before replacing the bulb with a new of the designated type. 2 Attaching the Lamp Socket Insert the plug into the socket, then push the guide pins gently into the guide holes. 10. Maintenance of the microscope 1. Clean all glass components by wiping gently with gauze.
