Instruction Manual

ROUTINE MAINTENANCE

Estimation of Filter Condition

The negative pressure inside the box will give a good indication of filter and system

condition. Set blower speed dial to the speed that gives the proper negative pressure conditions

inside the box. Record both the blower speed dial setting and the pressure reading on the gauge.

If box pressure begins to drop due to filter loading, increase the blower speed. To check filters,

reduce the blower speed dial setting to the setting recorded when the filters were clean. Read the

differential pressure gauge. When the gauge reading is one-half of the initial reading, replace

filters.

Filter Replacement

When filters become contaminated or after a predetermined length of time in use, the filter

should be replaced. Only experienced personnel, competent in the procedure of filter replacement

should perform this procedure.

Suggested Plan for Decontamination

Activities such as filter changing, service work, moving the cabinet, or gross spillage

generally require a gas decontamination procedure.

ONLY EXPERIENCED PERSONNEL COMPETENT IN THIS

PROCEDURE SHOULD BE USED.

LABCONCO strongly recommends reference to the Laboratory Safety Monograph (a

supplement to the NIH Guidelines for Recombinant DNA Research), published by the U.S.

Department of Health, Education and Welfare, January 1979.

Reference may also be made to the Formaldehyde Decontamination booklet available from

the U.S. Department of Health, Education and Welfare, Public Health Service, National Institute of

Health, National Cancer Institute.

For decontamination procedures with formaldehyde, refer to the following information.

1. NCI Safety Standards for Research Involving Chemical Carcinogens. Publication No.

(NIH) 76-900, 1975.

2. Carcinogen Safety Monographs, NCI Office of Research Safety.

3. “Potential Hazard of Tissue Culture Assays Arising from Carcinogenic Compounds

Incompletely Removed by Washing” E. B. Sansone, J. A. Poiley, R. J. Pienta and W. B.

Lebberz, III, Cancer Research 36.2455 – 1976.

4. “Recommended Decontamination Procedures for Aflatoxin”, L. Stoloff and W. Trager.

Journal of the Association of Official Agricultural Chemists, 48.681 – 1965.