Manual

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1200 COLORIMETER

AMMONIA-NITROGEN

CODE 3680-01

QUANTITY CONTENTS CODE

30 mL Ammonia Nitrogen Reagent #1 V-4797-G

3 x 30 mL *Ammonia Nitrogen Reagent #2 *V-4798-G

1 Pipet, 1 mL, plastic 0354

1 Colorimeter Tubes, with caps 0290-6

1 Water Sample Collecting Bottle 0688

1 1200 Colorimeter for Ammonia Nitrogen 26737

*WARNING: Reagents marked with an * are considered to be potential health hazards. To view

or print a Material Safety Data Sheet (MSDS) for these reagents go to lamotte.com. To obtain a

printed copy, contact LaMotte by e-mail, phone or fax.

To or der in di vid ual re agents or test kit com po nents, use the spec i fied code num ber.

INTRODUCTION

Ammonia nitrogen is present in various concentrations in many surface and ground

water supplies. Any sudden change in the concentration of ammonia nitrogen in a water

supply is cause for suspicion. A product of microbiological activity, ammonia nitrogen is

sometimes accepted as chemical evidence of pollution when encountered in natural

waters.

Ammonia is rapidly oxidized in natural water systems by special bacterial groups that

produce nitrite and nitrate. This oxidation requires that dissolved oxygen be available in

the water. Ammonia is an additional source of nitrogen as a nutrient which may

contribute to the expanded growth of undesirable algae and other forms of plant growth

that overload the natural system and cause pollution.

Summary of content (4 pages)

PAGE 1
200 COLORIMETER AMMONIA-NITROGEN CODE 3680-01 QUANTITY CONTENTS CODE 30 mL 3 x 30 mL 1 1 1 1 Ammonia Nitrogen Reagent #1 *Ammonia Nitrogen Reagent #2 Pipet, 1 mL, plastic Colorimeter Tubes, with caps Water Sample Collecting Bottle 1200 Colorimeter for Ammonia Nitrogen V-4797-G *V-4798-G 0354 0290-6 0688 26737 *WARNING: Reagents marked with an * are considered to be potential health hazards. To view or print a Material Safety Data Sheet (MSDS) for these reagents go to lamotte.com.
PAGE 2
AMMONIA NITROGEN TEST PROCEDURE: NESSLER METHOD Read the 1200 Colorimeter Manual before proceeding. Carefully wipe tubes dry before inserting into the colorimeter chamber. AMMONIA NITROGEN 1. 3. 5. 7. 2. Fill the Water Sample Collecting Bottle (0688) with sample water. This will be used to dispense sample water for the tests. Rinse and fill a colorimeter tube (0290) to the 10 mL line with sample water. Cap and wipe dry. 4. Push the READ button to turn the meter on.
PAGE 3
NOTE: For the best possible results, carry a reagent blank through the procedure. After scanning the blank in Step 4, perform the test procedure on clear, colorless, distilled or deioized water. Subtract results of regent blank from all subsequent test results. NOTE: If the reading displays Er2, repeat procedure on diluted sample, and multiply the result by the appropriate dilution factor. See 1200 Colorimeter Instruction Manual for procedure.
PAGE 4
FOR EXAMPLE: A fresh water sample at 20°C has a pH of 8.5 and the test result is 1.0 ppm as total Ammonia-Nitrogen. 1. The percentage from the table is 11.18% (or 0.1118). 2. 1 ppm total Ammonia-Nitrogen x 0.1118 = 0.1118 ppm Unionized Ammonia-Nitrogen 3. Total Ammonia-Nitrogen Unionized Ammonia-Nitrogen Ionized Ammonia-Nitrogen 1.0000 ppm - 0.1118 ppm = 0.8882 ppm AMMONIA NITROGEN TEST METHOD SPECIFICATIONS APPLICATION Drinking , surface, and saline waters; domestic and industrial wastes. RANGE 0 to 5.