SMARTSpectro ••••••••••••••••••••••••••••••••••••••••••••••• Spectrophotometer OPERATOR’S MANUAL L Mott v2.2 • Printed-04.
TABLE OF CONTENTS GENERAL INFORMATION Packaging & Delivery ······································································5 General Precautions ········································································5 Safety Precautions ···········································································5 Limits of Liability ·············································································5 Limited Warranty············································································
TABLE OF CONTENTS (cont.
GENERAL INFORMATION n PACKAGING & DELIVERY Experienced packaging personnel at LaMotte Company assure adequate protection against normal hazards encountered in transportation of shipments. After the product leaves the manufacturer, all responsibility for its safe delivery is assured by the transportation company. Damage claims must be filed immediately with the transportation company to receive compensation for damaged goods.
n LIMITED WARRANTY This instrument is guaranteed to be free from defects in material and workmanship for a period of one (1) years from original purchase date. In the event that a defect is found during the warranty time frame, LaMotte Company agrees that it will be repaired or replaced without charge except for the transporation costs. This guarantee does not cover batteries. This product can not be returned without a return authorization number from Lamotte Company.
n STATISTICAL AND TECHNICAL DEFINITIONS RELATED TO PRODUCT SPECIFICATIONS Method Detection Limit (MDL): “The method detection limit (MDL) is defined as the minimum concentration of a substance that can be measured and reported with 99% confidence that the analyte concentration is greater than zero and is determined from analysis of a sample in a given matrix containing the analyte.”1 Note that, “As Dr.
Sensitivity: Sensitivity is the resolution based on how this term is used in LaMotte catalogs. This term is not listed in any of the references. Sometimes it is used for detection limit. It is a confusing term and should be avoided. Repeatability: Repeatability is the within-run precision.6 A run is a single data set, from set up to clean up. Generally, one run occurs on one day.
n EPA COMPLIANCE The SMART Spectro is an EPA-Accepted instrument. EPA-Accepted means that the instrument meets the requirements for instrumentation as found in test procedures that are approved for the National Primary Drinking Water Regulations (NPDWR) or National Pollutant Discharge Elimination System (NPDES) compliance monitoring programs. EPA-Accepted instruments may be used with approved test procedures without additional approval.
CHEMICAL TESTING n WATER SAMPLING FOR CHEMICAL ANALYSIS Taking Representative Samples The underlying factor to be considered for any type of water sampling is whether or not the sample is truly representative of the source. To properly collect a representative sample: n l Sample as frequently as possible. l Collect a large sample or at least enough to conduct whatever tests are necessary. l Make a composite sample for the same sampling area.
this depth for a few minutes. The water percolates into the sample chamber displacing the air which bubbles to the surface. When the bubbles cease to rise, the device has flushed itself approximately five times and it may be raised to the surface for examination. The inner chamber of the sampling device is lifted out and portions of the water sample are carefully dispensed for subsequent chemical analysis.
n AN INTRODUCTION TO COLORIMETRIC ANALYSIS & SPECTROSCOPY Most test substances in water are colorless and undetectable to the human eye. To test for their presence we must find a way to “see” them. The LaMotte SMART Spectro can be used to measure any test substance that is itself colored or can be reacted to produce a color. In fact a simple definition of colorimetry is “the measurement of color” and a colorimetric method is “any technique used to evaluate an unknown color in reference to known colors”.
for a test factor is the complementary color of the test sample. For example the Nitrate-Nitrogen test produces a pink color proportional to the nitrate concentration in the sample (the greater the nitrate concentration, the darker the pink color). A wavelength in the green region should be selected to analyze this sample since a pinkish-red solution absorbs mostly green light.
Below is a list of suggested wavelength ranges for the color of the reacted samples. Use these as a starting point. Sample Color Wavelength Range Yellow 350-450 Yellow-Orange 450-490 Orange 490-510 Pink 510-570 Red 570-600 Green and Blue 600-750 n CALIBRATION CURVES The SMART Spectro contains precalibrated tests for the LaMotte reagent systems (see page 49). The first step in using a non-LaMotte reagent system with your SMART Spectro is to create a calibration curve for the reagent system.
5. Plot results on graph paper or computer using any available plotting program. If results are as %T versus concentration, semilog graph paper must be used. Plot the standard solution concentrations on the horizontal, linear axis, and the %T on the vertical, logarithmic axis. If results are as absorbance versus standard solution concentration, simple linear graph paper can be used. Plot the standard solution concentration on the horizontal axis, and the absorbance on the vertical axis. 6.
n PREPARING DILUTE STANDARD SOLUTIONS Standard solutions should be prepared to create a calibration curve. Standard solutions can be prepared by diluting a known concentrated standard by specified amounts. A chart or computer spreadsheet can be created to determine the proper dilutions. Use volumetric flasks and volumetric pipets for all dilutions. 1. In Column A – Record the maximum concentration of test as determined by the range and path length. 2.
For example, a 10.0 mL water sample was determined to contain 0.3 ppm iron. To a second 10.0 mL sample, 0.1 mL of 50 ppm iron standard was added. The concentration of iron due to the “spike” was (0.10 mL x 50 ppm)/10.0 mL = 0.50 ppm. The concentration of iron determined in the spiked sample should be 0.3 + 0.5 = 0.8 ppm iron. (Note: any error due to the increased volume from the “spike” is negligible).
n INTERFERENCES LaMotte reagent systems are designed to minimize most common interferences. Each individual test instruction discusses interferences unique to that test. Be aware of possible interferences in the water being tested. The reagent systems also contain buffers to adjust the water sample to the ideal pH for the reaction. It is possible that the buffer capacity of the water sample may exceed the buffer capacity of the reagent system and the ideal pH will not be obtained.
OPERATION OF THE SMART SPECTRO n OVERVIEW The SMART Spectro is a portable, microprocessor controlled, direct reading, single beam spectrophotometer. It has a 4 line, 20 character liquid crystal display for alphabetical and numerical messages. The operation is controlled with the keypad through menu driven software in response to selections shown on the display. The test library consists of 100 LaMotte tests (not all 100 may be available at present) and 25 “User Tests”.
n COMPONENTS Figure 1 shows a diagram of the SMART Spectro and the components. AC adapter socket RS232 serial port 10mm square cell in Adapter 25mm round cell in Universal Adapter Display LAMOTTE SMART SPECTRO Chamber PRESS ENTER Scrolling arrows for scrolling between choices Scrolling For arrows for making scrolling selections between choices ENTER OFF Turning off the spectro 20 EXIT For exiting menus ON For turning on the spectro SMART SPECTRO SPECTROPHOTOMETER 05.
n QUICK START Some quick instructions to get into testing. 1. Press ON to turn on the SMART Spectro. The WELCOME screen will appear for about 2 seconds. LAMOTTE SMART Version 1.0 May 2000 2. The MAIN MENU screen will appear automatically. MAIN MENU 12:45 * CALIBRATE WL PROGRAMMED TESTS %T/ABS 3. Press t until the Q is next to PROGRAMMED TESTS. MAIN MENU 00:00 CALIBRATE WL * PROGRAMMED TESTS %T/ABS 4. Press ENTER/Q to select PROGRAMMED TESTS. PROGRAMMED TESTS menu will appear.
8. Press ENTER/Q to select test. 15 Chlorine * SCAN BLANK SCAN SAMPLE END 515 NM 9. Insert blank, press ENTER/Q to scan blank. 15 Chlorine * SCAN BLANK SCAN SAMPLE END 515 NM 10. Insert reacted sample. Press ENTER/Q to scan sample. 15 Chlorine 16.5%T 0.7834 A 1.28 PPM PRINT PRESS ENTER After obtaining test results, scroll with t or s, and make another selection with ENTER/Q. Press EXIT to escape to previous menus. 22 SMART SPECTRO SPECTROPHOTOMETER 05.
GENERAL OPERATING PROCEDURES The operation of the SMART Spectro is controlled by a microprocessor. The microprocessor is programmed with menu driven software. A menu is a list of choices. This allows a selection of various tasks for the spectrophotometer to perform, such as, scan blank, scan sample, and edit test sequences. The keypad is used to make menu selections which are viewed in the display.
n THE DISPLAY & THE MENUS The display allows menu selections to be viewed and chosen. These choices instruct the spectrophotometer to perform specific tasks. The menus are viewed in the display using a general format which is followed from one menu to the next. Each menu is a list of choices or selections. There are four lines in the display. The top line in each menu is a title or pertinent instruction. The top line does not change unless a new menu is selected. The second line is used in two ways.
CALIBRATION n CALIBRATE WAVELENGTH The Calibrate Wavelength (CALIBRATE WL) mode is used to establish or re-establish the accuracy of the wavelength selection process. Normally, the Calibrate Wavelength procedure should be run after the SMART Spectro is turned ON and allowed to warm up for 15 minutes or if operating conditions (temperature, humidity, etc.) change significantly. For field use, when operating with the battery, calibrate wavelength prior to going into the field using AC power.
The Calibrate Wavelength procedure takes about 1-2 minutes to be completed. During the calibration, the Spectro will display two numbers at the bottom of the screen. The first number is fixed. The second number will change and can have a range of values. The microprocessor will move the grating in search of the position that gives a very specific maximum light intensity. The microprocessor will then move the grating a precise predetermined amount from this position.
n SEQUENCES OF TESTS SEQUENCE 1, SEQUENCE 2, and SEQUENCE 3 are alterable sequences. They may be edited using the EDIT function mode. Any of the LaMotte pre-programmed tests or User Tests may be placed in these sequences in whatever testing order that is preferred. Some examples of typical sequences are given below.
n GENERAL TESTING PROCEDURES The following are some step by step examples of how to run tests from the PROGRAMMED TESTS menu. These test procedures are designed to be used with LaMotte SMART Spectro reagent systems. n TESTING WITH THE LaMOTTE PROGRAMMED TESTS Scroll to ALL TESTS with the t button in the PROGRAMMED TESTS menu. Press the ENTER/Q button to select ALL TESTS. Press ENTER/Q button to select 1 Aluminum. PROGRAMMED TESTS SEQUENCE 2 SEQUENCE 3 * ALL TESTS ALL TESTS * 1. Aluminum 2.
The spectrophotometer will scan the sample and the results screen will appear. 1 Aluminum 99.8%T 0.0015A 0.01 PPM PRINT PRESS ENTER Press ENTER/Q to print the result when connected to a printer or computer. To repeat the test, press EXIT to escape to the test screen, then press the ENTER/Q button to scan the sample again. The last blank scanned is used to zero the spectrophotometer for repeat scans.
SETUP & EDIT SEQUENCES & USER TESTS The EDIT menu allows any of the three alterable test sequences (SEQUENCE 1, SEQUENCE 2, and SEQUENCE 3) and any of the 25 User Tests in the ALL TESTS fixed sequence to be edited. This feature allows a sequence or test which is used frequently to be set-up for easy access. The order of the sequence can be arranged to suit the needs of the user. Any combination, and any order of tests from ALL TESTS (including User Tests), may be placed into these sequences.
n ADDING OR DELETING TESTS There are two ways to alter a sequence - INSERT and DELETE. INSERT is used to add a new test to a sequence and to place the new test before an existing test in a sequence. DELETE is used to remove an existing test from a sequence. n Below is a step by step example of how to add a test to SEQUENCE 3 starting from the EDIT menu. Use the t button to scroll to EDIT SEQUENCE 3 in the EDIT menu. Press ENTER/Q button to select EDIT SEQUENCE 3.
Press ENTER/Q button to select Phosphate L. EDIT SEQUENCE 3 * 78 Phosphate L END OF LIST Press ENTER/Q to insert a test before PHOSPHATE L. EDIT SEQUENCE 3 * INSERT DELETE 78 Phosphate L Press ENTER/Q to select 1 Aluminum from the list and insert it before Phosphate L. The display will automatically return to EDIT SEQUENCE 3 menu. INSERT BEFORE * 1 Aluminum 2 Alkalinity-TT 3 Ammonia-N L F : : : END OF LIST SEQUENCE 3 has now been modified and will remain until it is edited again.
n Below is an example of how to delete a test from SEQUENCE 3, which was just created, starting from the EDIT menu. Use the t button to scroll to EDIT SEQUENCE 3 in the EDIT menu. Press ENTER/Q button to select EDIT SEQUENCE 3. EDIT EDIT SEQUENCE 1 EDIT SEQUENCE 2 * EDIT SEQUENCE 3 Press ENTER/Q button to select 1 Aluminum for deletion. EDIT SEQUENCE 3 * 1 Aluminum 78 Phosphate L END OF LIST Use the t button to scroll to DELETE.
n EDIT USER TESTS If a test other than the LaMotte programmed tests is performed regularly, a calibration for it may be entered in one of the 25 User Tests. These tests are originally named “User Test 1 - 25". It will be possible to rename the test, select a wavelength, enter a new calibration and select the number of decimal places used to display the results. A User Test may be added for a reagent system for which no precalibrated test exists.
From the EDIT USER TESTS menu, select the User Test to be entered or changed. In this example, choose 101 User Test 1. Use the t and s buttons to scroll to other User Tests if desired. Select the USER TEST by pressing the ENTER/Q button. EDIT USER TESTS * 101 User Test 1 102 User Test 2 103 User Test 3 104 User Test 4 : END OF LIST NOTE: This menu allows a test to be renamed, a wavelength to be selected, reacted standards to be scanned and the numerical format of the displayed test result to be selected.
n NAMING THE TEST A NAME can be up to 14 characters long. The menu choices for each character are 26 letters A to Z, ten numerals 0 to 9, a space, a dash, a decimal point, and a ! as a terminator. Selecting the terminator indicates the end of the name and stores the name. The terminator, !, is the first menu choice since it is the one character that will always have to be selected. It is before the letter A. DO NOT CHOOSE THE ! UNTIL THE NAMING OF THE TEST HAS BEEN COMPLETED.
n SELECTING THE WAVELENGTH Scroll to SELECT WL. Press ENTER/Q to choose SELECT WL. Use s and t to scroll to the appropriate WAVELENGTH. 101 H2O NAME THE TEST * SELECT WL NEW CALIBRATION FORM AT RESULT SELECT WAVELENGTH 600 NM SET PRESS ENTER Press ENTER/Q to select the wavelength. Display goes back to 101 H2O screen. SMART SPECTRO SPECTROPHOTOMETER 05.
n ENTERING A NEW CALIBRATION Scroll to NEW CALIBRATION. Press the ENTER/Q button. The INPUT DATA NUMBERS menu appears. Using the s and t buttons set the number of data points to be used. When finished press the ENTER/Q button. The NEW CALIBRATION menu appears. The NEW CALIBRATION menu allows the entry of the data points. Select ENTER STD.1 by pressing ENTER/Q button while the * is adjacent to ENTER STD.1 in the display.
The * moves automatically to the STD 1.= line. Place the reacted standard into sample chamber beginning with the lowest concentration. Use the s and t buttons to enter the concentration of the standard by selecting the characters with the blinking cursor. After entering the concentration, select the ! terminator. The standard will then be scanned. The absorbance will be displayed. NOTE: Sample must be in chamber when ! is selected. ENTER STD.1 BLANKED * STD.1. = 1.0 !0123456789. ENTER STD.1 BLANKED STD.
When finished inputting standards scroll down (in the NEW CALIBRATION menu) to the CALCULATE selection. Select CALCULATE. NEW CALIBRATION ENTER STD.7 ENTER STD.8 * CALCULATE From the SELECT DEGREES menu scroll to and select either 1 DEGREE THRU.0 or 1 DEGREE. SELECT DEGREES * 1 DEGREE THRU.0 1 DEGREE NOTE: 1 DEGREE THRU.0 calculates the best straight line fit through the data points and intersects with the origin at 0 ppm, 0 absorbance. This is a classical Beers Law calibration.
n SELECTING THE NUMERICAL FORMAT OF THE RESULT To input tests with very different ranges, the number of decimal places displayed for a result can be selected. A test which ranges from 20 to 1000 ppm should not be displayed with three decimal places. A test with a range from 0.010 to 0.500 needs three decimal places (the microprocessor will always calculate the concentration to many more significant figures than will be displayed). Menu choices of 0, 1, 2, or 3 decimal places will be given for the display.
Press EXIT button again to escape to EDIT menu and once again to escape to PROGRAMMED TESTS menu. NOTE: Test 101 was USER TEST 1 and now is H2O. It is still a USER TEST because its calibration can be changed but it has a different name. 42 EDIT * EDIT SEQUENCE 1 EDIT SEQUENCE 2 EDIT SEQUENCE 3 PROGRAMMED TESTS * SEQUENCE 1 SEQUENCE 2 SEQUENCE 3 ALL TESTS EDIT END OF LIST SMART SPECTRO SPECTROPHOTOMETER 05.
MEASURING IN THE %T/ABS MODE Scroll to %T/ABS with the t button in the MAIN MENU. Press ENTER/Q button to select %T/ABS. MAIN MENU 00:00 CALIBRATE WL PROGRAMMED TESTS * %T/ABS The wavelength appears at the top right of the display. Press ENTER/Q button while * is next to SELECT WL in display to change the wavelength. %T/ABS 604 NM * SELECT WL SCAN BLANK SCAN SAMPLE END OF LIST Use the t s buttons to display the appropriate wavelength. Push ENTER/Q button to select that wavelength .
At this point, it is possible to scan another sample, scan another blank, or select another wavelength. To print the results to a connected computer or serial printer, press ENTER/Q and return to previous menu. Press EXIT to escape to previous menu. %T/ABS 530NM T=90.7%T A=0.0424A PRINT PRESS ENTER If no more samples are to be scanned, press EXIT to return to the MAIN MENU or press the OFF button to turn off the spectrophotometer.
PC LINK The SMART Spectro may be interfaced with any Windows-based program by using the LaMotte SMARTLink2 Program and Interface Cable (Code 1912-3 [3.5 disk] or 1912-CD [compact disk]). The program stores customer information and test data in a database. It can be used to download data stored in the SMART Spectro datalogger for each test site.
TEST MODE Select TEST MODE from the Main Menu. Test Mode allows the user to change the way that blanking and reading of results is carried out during the %T/ABS testing. It does not affect any of the Pre-programmed tests. REGULAR is the default test mode and should be used whenever the blank is clear or has less absorbance than the samples. This will be the case most of the time.
MAINTENANCE n CLEANING Clean with a damp, lint-free cloth. DO NOT ALLOW WATER TO ENTER THE SPECTROPHOTOMETER CHAMBER OR ANY OTHER PARTS OF THE METER. n LIGHT BULB The quartz halogen bulb in the SMART has an approximate life of 1000 hours. If a test is performed and there is no response and the unit is receiving adequate power, the light bulb may need to be replaced. With the meter power ON check the rear lamp access panel to see if any light can be observed through the vents.
TROUBLESHOOTING GUIDE n ERROR MESSAGES OUT OF RANGE If the message OUT OF RANGE is displayed when scanning a sample, the sample may be over range or under range. If the sample is over range the sample should be diluted and tested again (see Sample Dilution Techniques & Volumetric Measurements, page14). n BATTERY (optional) If the symbol BAT appears in the upper left corner of the display when using the battery pack, the battery needs to be charged.
n HELPFUL HINTS n POWER The power supply has an internal switch that handles both 110V and 220V input. STRAY LIGHT The SMART Spectro should have no problems with stray light. Make sure that the sample compartment lid is always fully closed. n ACCIDENTAL LOSS OF POWER If for any reason the meter experiences a loss of power during wavelength calibration, the next time the meter is powered up a wavelength calibration will automatically be performed.
SMARTSpectro ••••••••••••••••••••••••••••••••••••••••••••••• Spectrophotometer TEST INSTRUCTIONS L Mott v2.2 • Printed 04.
SPECTROPHOTOMETER n REAGENT SYSTEMS LIST LaMotte Company continuously updates the list of pre-programmed tests as the calibrations become available. Call LaMotte Technical Services at 1-800-344-3100 (410-778-3100 outside the USA) or e-mail at tech@lamotte.com for a current list of available calibrations.
Hydrogen PeroxideLow Range Hydrogen PeroxideHigh Range Hydrogen Peroxide-Shock Hydroquinone Iodine Iron-Bipyridyl Iron-UDV Iron-Phenanthroline Lead Manganese-Low Range Manganese-High Range Mercury Methylethylketoxime Molybdenum-High Range Nickel Nitrate Nitrogen-Low Range Nitrate-TesTab Nitrite Nitrogen-Low Range Nitrite-TesTab Nitrogen, Total Oxygen Scavengers (listed under individual tests) Ozone-Low Range Ozone-High Range pH-Chlorophenol Red pH-Phenol Red pH-Thymol Blue Phenol Phosphate-Low Range Phospha
ALKALINITY–UDV METHOD–UNIT DOSE VIALS • CODE 4318-H QUANTITY CONTENTS 1 Alkalinity Unit Dose Vials, 10 pouches CODE 4318-H Equipment needed but not supplied: STANDARD ACCESSORY PACKAGE • CODE 1961 1 1 1 Package of 3 Vials (empty) Syringe, 6 mL, plastic Foil Storage Bag 0156 1184 9467 Or: ADVANCED ACCESSORY PACKAGE • CODE 1962 1 1 1 1 1 Pipettor Pipet Tip (0-5 mL) Cuvette Rack Package of 3 Vials (empty) Foil Storage Bag 30528 30695 31695 0156 9467 *WARNING: Reagents marked with a * are considered
PROCEDURE Use 10 mm square cell adapter 1. Press and hold ON button until spectrophotometer turns on. 2. Scroll to and select PROGRAMMED TESTS. 3. Scroll to and select ALL TESTS (or another sequence containing 2 Alk-UDV) from TESTING MENU. 4. Scroll to and select 2 Alk-UDV from menu. 5. Rinse a clean vial (0156) with sample water. 6. Use the syringe (1184) to add 3 mL of sample to the vial. 7. Insert the vial into chamber, close lid and select SCAN BLANK. 8. Remove vial from Spectro. 9.
ALUMINUM ERIOCHROME CYANINE R METHOD • CODE 364I-SC QUANTITY 5g 2 x 120 mL 120 mL 15 mL 1 1 2 1 CONTENTS *Aluminum Inhibitor Reagent *Aluminum Buffer Reagent Aluminum Indicator Reagent Aluminum Complexing Reagent Spoon, 0.1 g, plastic Spoon, 0.05 g, plastic Pipets, 1.0 mL, plastic Test Tube, glass, 5 mL w/cap CODE *7865-C *7866-J 7867-J 7868-E 0699 0696 0354 0230 *WARNING: Reagents marked with a * are considered to be potential health hazards.
PROCEDURE Use universal sample holder. 1. Press and hold ON button until spectrophotometer turns on. 2. Scroll to and select PROGRAMMED TESTS. 3. Scroll to and select ALL TESTS (or another sequence containing 1 Aluminum). 4. Scroll to and select 1 Aluminum from menu. 5. Rinse a clean Spectro tube (0290) with sample water. Fill to the 10 mL line with sample. 6. Insert tube into Spectro chamber and select SCAN BLANK. 7. Rinse a clean test tube (0230) with sample water. Fill to the 5 mL line with sample. 8.
AMMONIA-NITROGEN–LOW RANGE SALICYLATE METHOD • CODE 3659-01-SC QUANTITY 60 mL 10 g 2x5g 1 1 1 CONTENTS *Salicylate Ammonia #1 *Salicylate #2 *Salicylate #3 Spoon, 0.1 g, plastic Spoon, 0.15 g, plastic Pipet, 1.0 mL, plastic CODE *3978-H *7457-D *7458-C 0699 0727 0354 *WARNING: Reagents marked with a * are considered to be potential health hazards. To view or print a Material Safety Data Sheet (MSDS) for these reagents see MSDS CD or www.lamotte.com.
PROCEDURE–FRESH WATER Use universal sample holder. 1. Press and hold ON button until spectrophotometer turns on. 2. Scroll to and select PROGRAMMED TESTS from menu. 3. Scroll to and select ALL TESTS (or another sequence containing 3 Ammonia-N LF) from TESTING MENU. 4. Scroll to and select 3 Ammonia-N LF from menu. 5. Rinse a clean tube (0290) with sample water. Fill to the 10 mL line with sample. 6. Insert tube into chamber, close lid and select SCAN BLANK. (See Note.) 7. Remove tube from Spectro.
PROCEDURE–SALT WATER Use universal sample holder. 1. Press and hold ON button until spectrophotometer turns on. 2. Scroll to and select PROGRAMMED TESTS from menu. 3. Scroll to and select ALL TESTS (or another sequence containing 4 Ammonia-N LS) from TESTING MENU. 4. Scroll to and select 4 Ammonia-N LS from menu. 5. Rinse a clean tube (0290) with sample water. Fill to the 10 mL line with sample. 6. Insert tube into chamber, close lid and select SCAN BLANK. (See Note.) 7. Remove tube from Spectro. Use the 1.
SMART SPECTRO TEST PROCEDURES 05.
AMMONIA-NITROGEN–HIGH RANGE NESSLERIZATION METHOD • CODE 3642-SC QUANTITY 30 mL 2 x 30 mL 1 CONTENTS Ammonia Nitrogen Reagent #1 *Ammonia Nitrogen Reagent #2 Pipet, 1 mL, plastic CODE V-4797-G *V-4798-G 0354 *WARNING: Reagents marked with a * are considered to be potential health hazards. To view or print a Material Safety Data Sheet (MSDS) for these reagents see MSDS CD or www.lamotte.com. To obtain a printed copy, contact LaMotte by e-mail, phone or fax.
PROCEDURE Use universal sample holder. 1. Press and hold ON button until spectrophotometer turns on. 2. Scroll to and select PROGRAMMED TESTS. 3. Scroll to and select ALL TESTS (or another sequence containing 5 Ammonia-N H) from TESTING MENU. 4. Scroll to and select 5 Ammonia-N H from menu. 5. Rinse a clean tube (0290) with sample water. Fill to the 10 mL line with sample. 6. Insert tube into chamber, close lid and select SCAN BLANK. (See Note) 7. Remove tube from Spectro.
BIGUANIDE COLORIMETRIC METHOD • CODE 4044 QUANTITY CONTENTS 2 X 60 mL 1 Biguanide Indicator Pipet, plastic, 1.0 mL CODE 3994-H 0354 Biguanide is a non-chlorine, non-bromine chemical sanitizer. It is more stable than chlorine or bromine and has little chemical odor. Biquanide is an effective bacteriacide but, unlike chlorine and bromine, it does not destroy organic contaminants. Therefore, hydrogen peroxide is added to biguanide pools on a regular basis to eliminate organic contaminants.
PROCEDURE Use universal sample holder 1. Press and hold ON button until spectrophotometer turns on. 2. Scroll to and select PROGRAMMED TESTS. 3. Select ALL TESTS (or another sequence containing 7 Biguanide from TESTING MENU. 4. Scroll to and select 7 Biguanide from menu. 5. Rinse a tube (0290) with sample water. Fill to 10 mL with sample. 6. Insert the tube into chamber, close lid and select SCAN BLANK. 7. Remove the tube from colorimeter. 8. Use the 1.0 mL pipet (0354) to add 2.
BORON AZOMETHINE-H METHOD • CODE 4868 QUANTITY 120 mL 10 g 1 1 1 CONTENTS *Boron Buffer *Boron Indicator Powder Pipet, plastic, 1.0 mL Spoon, 0.15 g Dark Storage Chamber, brown CODE *4869-J *4870-D 0354 0727 0108 *WARNING: Reagents marked with a * are considered to be potential health hazards. To view or print a Material Safety Data Sheet (MSDS) for these reagents see MSDS CD or www.lamotte.com. To obtain a printed copy, contact LaMotte by e-mail, phone or fax.
PROCEDURE Use universal sample holder 1. This test requires a Reagent Blank. Rinse a tube (0290) with clear, colorless, boron free water. Fill to 10 mL line with clear, colorless, boron free water. 2. Use the 1.0 mL pipet (0354) to add 2 mL of *Boron Buffer (4869). Cap and mix. 3. Use the 0.15 g spoon (0727) to add one level measure of *Boron Indicator Powder (4870). Press full spoon against side of jar to compress powder. Scrape off excess powder on inside neck of bottle. Tap excess off spoon handle. 4.
CADMIUM PAN METHOD • CODE 4017 QUANTITY CONTENTS 60 mL 15 mL 30 mL 30 mL 1 2 Buffered Ammonia Reagent Sodium Citrate, 10% PAN Indicator Stabilizing Reagent Pipet, 1.0 mL, plastic Pipet, 0.5 mL, plastic CODE 4020-H 6253-E 4021-G 4022-G 0354 0353 *WARNING: Reagents marked with a * are considered to be potential health hazards. To view or print a Material Safety Data Sheet (MSDS) for these reagents see MSDS CD or www.lamotte.com. To obtain a printed copy, contact LaMotte by e-mail, phone or fax.
PROCEDURE Use universal sample holder. 1. Press and hold ON button until spectrophotometer turns on. 2. Scroll to and select PROGRAMMED TESTS. 3. Scroll to and select ALL TESTS (or another sequence containing 12 Cadmium) from TESTING MENU. 4. Scroll to and select 12 Cadmium from menu. 5. Rinse a tube (0290) with sample water. Fill to the 10 mL line with sample. 6. Insert the tube into chamber, close lid and select SCAN BLANK. 7. Remove tube from Spectro. Use the 1.0 mL pipet (0354) to add 1.
CALCIUM & MAGNESIUM (TOTAL) HARDNESS–UDV UNIT DOSE VIALS • CODE 4309-H QUANTITY CONTENTS CODE 1 Calcium Hardness Unit Dose Vials, 10 pouches 4309-H Equipment needed but not supplied: STANDARD ACCESSORY PACKAGE • CODE 1961 1 1 1 Package of 3 Vials (empty) Syringe, 3 mL, plastic Foil Storage Bag 0156 1184 9467 Or: ADVANCED ACCESSORY PACKAGE • CODE 1962 1 1 1 1 1 Pipettor Pipet Tips (0-5 mL) Cuvette Rack Package of 3 Vials (empty) Foil Storage Bag 30528 30695 31695 0156 9467 *WARNING: Reagents mark
PROCEDURE Use 10 mm square cell adapter 1. Press and hold ON button until spectrophotometer turns on. 2. Scroll to and select PROGRAMMED TESTS. 3. Scroll to and select ALL TESTS (or another sequence containing 13 Ca&Mg Hard-UDV) from TESTING MENU. 4. Scroll to and select 13 Ca&Mg Hard-UDV from menu. 5. Rinse a clean vial (0156) with sample water. 6. Use the syringe (1184) to add 3mL of sample to the vial. 7. Insert the vial into chamber, close lid and select SCAN BLANK. 8. Remove vial from Spectro. 9.
CHLORIDE ARGENTOMETRIC METHOD • CODE 3693-SC QUANTITY 50 1 CONTENTS CODE *Chloride Spectrophotometric Grade Tablets Tablet Crusher 3885A-H 0175 *WARNING: Reagents marked with a * are considered to be potential health hazards. To view or print a Material Safety Data Sheet (MSDS) for these reagents see MSDS CD or www.lamotte.com. To obtain a printed copy, contact LaMotte by e-mail, phone or fax. Chloride is one of the major anions found in water and sewage.
PROCEDURE Use universal sample holder 1. Press and hold ON button until spectrophotometer turns on. 2. Scroll to and select PROGRAMMED TESTS. 3. Scroll to and select ALL TESTS (or another sequence containing 21 Chloride-TT) from TESTING MENU. 4. Scroll to and select 21 Chloride-TT from menu. 5. Rinse a tube (0290) with sample water. Fill to 10 mL with sample. 6. Insert the tube into chamber, close lid and select SCAN BLANK. 7. Remove the tube from Spectro. 8.
CHLORINE LIQUID DPD METHOD • CODE 4859 QUANTITY 30 mL 30 mL 30 mL CONTENTS DPD 1A Free Chlorine Reagent *DPD 1B Free Chlorine Reagent *DPD 3 Total Chlorine Reagent CODE P-6740-G *P-6741-G *P-6743-G *WARNING: Reagents marked with a * are considered to be potential health hazards. To view or print a Material Safety Data Sheet (MSDS) for these reagents see MSDS CD or www.lamotte.com. To obtain a printed copy, contact LaMotte by e-mail, phone or fax.
SAMPLE Chlorine in aqueous solutions is not stable, and the chlorine HANDLING & content of samples or solutions, particularly weak solutions, PRESERVATION: will rapidly decrease. Exposure to sunlight or agitation will accelerate the reduction of chlorine present in such solutions. For best results, start analysis immediately after sampling. Samples to be analyzed for chlorine cannot be preserved or stored. INTERFERENCE: The only interfering substance likely to be encountered in water is oxidized manganese.
PROCEDURE–FREE CHLORINE Use universal sample holder 1. Press and hold ON button until spectrophotometer turns on. 2. Scroll to and select PROGRAMMED TESTS. 3. Scroll to and select ALL TESTS (or another sequence containing 17 Cl DPD-Liq) from TESTING MENU. 4. Scroll to and select 17 Cl DPD-Liq from menu. 5. Rinse a tube (0290) with sample water. Fill to the 10 mL line with sample. 6. Insert the tube into chamber, close lid and select SCAN BLANK. 7. Remove the tube from spectrophotometer. 8.
SMART SPECTRO TEST PROCEDURES 05.
CHLORINE–BROMINE–IODINE DPD METHOD • CODE 3643-SC QUANTITY 100 100 15 mL 1 CONTENTS CODE *Chlorine DPD #1 Instrument Grade Tablets *Chlorine DPD #3 Instrument Grade Tablets Glycine Solution Tablet Crusher *6903A-J *6197A-J 6811-E 0175 *WARNING: Reagents marked with a * are considered to be potential health hazards. To view or print a Material Safety Data Sheet (MSDS) for these reagents see MSDS CD or www.lamotte.com. To obtain a printed copy, contact LaMotte by e-mail, phone or fax.
SAMPLE Chlorine in aqueous solutions is not stable, and the chlorine HANDLING & content of samples or solutions, particularly weak solutions, PRESERVATION: will rapidly decrease. Exposure to sunlight or agitation will accelerate the reduction of chlorine present in such solutions. For best results, start analysis immediately after sampling. Samples to be analyzed for chlorine cannot be preserved or stored. INTERFERENCE: The only interfering substance likely to be encountered in water is oxidized manganese.
PROCEDURE–FREE CHLORINE Use universal sample holder. 1. Press and hold ON button until spectrophotometer turns on. 2. Scroll to and select PROGRAMMED TESTS. 3. Scroll to and select ALL TESTS (or another sequence containing 15 Chlorine) from TESTING MENU. 4. Scroll to and select 15 Chlorine from menu. 5. Rinse a clean tube (0290) with sample water. Fill to the 10 mL line with sample. 6. Insert tube into chamber, close lid and select SCAN BLANK. 7.
BROMINE Like chlorine, bromine is an effective germicidal agent employed in drinking water treatment, pool and spa water sanitation, food service sanitation, and other public health applications. APPLICATION: Drinking, surface, and saline waters; swimming pool water; domestic and industrial waters and wastes. RANGE: 0.00–9.00 ppm Bromine METHOD: In buffered sample bromine reacts with diethyl-p-phenylene diamine (DPD) to produce a pink-red color in proportion to the concentration of bromine present.
PROCEDURE A: BROMINE (NO CHLORINE) Use universal sample holder. 1. Press and hold ON button until spectrophotometer turns on. 2. Scroll to and select PROGRAMMED TESTS. 3. Scroll to and select ALL TESTS (or another sequence containing 9 Bromine-LR) from TESTING MENU. 4. Scroll to and select 9 Bromine-LR from menu. 5. Rinse a clean tube (0290) with sample water. Fill to the 10 mL line with sample. 6. Insert tube into chamber, close lid and select SCAN BLANK. 7. Remove tube from Spectro.
PROCEDURE B: BROMINE IN THE PRESENCE OF CHLORINE Use universal sample holder. 1. Press USE button to turn on colorimeter. 2. Scroll to and select ALL TESTS (or another sequence containing 9 Bromine-LR) from TESTING MENU. 3. Scroll to and select 9 Bromine-LR from menu. 4. Rinse a clean tube (0290) with sample water. Fill to the 10 mL line with sample. 5. Insert tube into chamber close lid and select SCAN BLANK. 6. Rinse a second clean tube (0290) with sample water. Fill to the 10 mL line with sample.
IODINE Like chlorine and bromine, iodine is an effective germicidal agent employed in drinking water treatment, pool and spa water sanitation, food service sanitation, and other public health applications. APPLICATION: Drinking, surface, and saline waters; swimming pool water; domestic and industrial wastes. RANGE: 0.00–14.00 ppm Iodine METHOD: In a buffered sample iodine reacts with diethyl-p-phenylene-diamine (DPD) to produce a pink-red color in proportion to the concentration of iodine present.
PROCEDURE Use universal sample holder. 1. Press and hold ON button until spectrophotometer turns on. 2. Scroll to and select PROGRAMMED TESTS. 3. Scroll to and select ALL TESTS (or another sequence containing 50 Iodine) from TESTING MENU. 4. Scroll to and select 50 Iodine from menu. 5. Rinse a clean tube (0290) with sample water. Fill tube to the 10 mL line with sample. 6. Insert tube into chamber, close lid and select SCAN BLANK. 7. Remove tube from Spectro.
CHLORINE DIOXIDE DPD METHOD • CODE 3644-SC QUANTITY 100 15 mL 1 CONTENTS CODE *Chlorine DPD #1 Instrument Grade Tablets Glycine Solution Tablet Crusher *6903A-J 6811-E 0175 *WARNING: Reagents marked with a * are considered to be potential health hazards. To view or print a Material Safety Data Sheet (MSDS) for these reagents see MSDS CD or www.lamotte.com. To obtain a printed copy, contact LaMotte by e-mail, phone or fax.
8. Add one *Chlorine DPD #1 Instrument Grade Tablet (6903). Crush tablet with tablet crusher. Cap and shake until tablet dissolves. Fill to 10 mL line with sample water. Solution will turn pink if chlorine dioxide is present. Wait 15 seconds, but no longer than 30 seconds. Mix. 9. Insert tube into chamber, close lid and select SCAN SAMPLE. 10. Press OFF button to turn spectrophotometer off or press EXIT button to exit to a previous menu or make another menu selection. SMART SPECTRO TEST PROCEDURES 05.
CHLORINE, FREE – UDV DPD METHOD–UNIT DOSE VIALS • CODE 4311-H QUANTITY 1 CONTENTS CODE *Free Chlorine Unit Dose Vials, 10 pouches 4311-H Equipment needed but not supplied: STANDARD ACCESSORY PACKAGE • CODE 1961 1 1 1- Package of 3 Vials (empty) Syringe, 3 mL, plastic 0156 1184 Or: ADVANCED ACCESSORY PACKAGE • CODE 1962 1 1 1 1 1 Pipettor Pipet Tips (0-5 mL) Cuvette Rack Package of 3 Vials (empty) Foil Storage Bag 30528 30695 31695 0156 9467 *WARNING: Reagents marked with a * are considered to be
Chlorine can be added in the form of chlorine gas, liquid sodium hypochlorite (bleach), granular calcium hypochlorite or as organic chlorine compounds. Chlorine is not present in natural water supplies; if it is present it is the result of chlorination of a water supply or of chlorinated compounds being discharged as waste from industrial operations. The presence of chlorine in concentrations above 0.
PROCEDURE Use 10 mm square cell adapter 1. Press and hold ON button until spectrophotometer turns on. 2. Scroll to and select PROGRAMMED TESTS. 3. Scroll to and select ALL TESTS (or another sequence containing 16 Cl Free-UDV) from TESTING MENU. 4. Scroll to and select 16 Cl Free-UDV from menu. 5. Rinse a clean vial (0156) with sample water. 6. Use the syringe (1184) to add 3mL of sample to the vial. 7. Insert the vial into chamber, close the lid and select SCAN BLANK. 8. Remove the vial from the Spectro. 9.
SMART SPECTRO TEST PROCEDURES 05.
TOTAL CHLORINE–UDV DPD METHOD–UNIT DOSE VIALS • CODE 4312-H QUANTITY 1 CONTENTS CODE *Total Chlorine Unit Dose Vials, 10 pouches 4312-H Equipment needed but not supplied: STANDARD ACCESSORY PACKAGE • CODE 1961 1 1 1 Package of 3 Vials (empty) Syringe, 3 mL, plastic Foil Storage Bag 0156 1184 9467 Or: ADVANCED ACCESSORY PACKAGE • CODE 1962 1 1 1 1 1 Pipettor Pipet Tips (0-5 mL) Cuvette Rack Package of 3 Vials (empty) Foil Storage Bag 30528 30695 31695 0156 9467 *WARNING: Reagents marked with a *
Chlorine can be added in the form of chlorine gas, liquid sodium hypochlorite (bleach), granular calcium hypochlorite or as organic chlorine compounds. Chlorine is not present in natural water supplies; if it is present it is the result of chlorination of a water supply or of chlorinated compounds being discharged as waste from industrial operations. The presence of chlorine in concentrations above 0.
PROCEDURE Use 10 mm square cell adapter 1. Press and hold ON button until spectrophotometer turns on. 2. Scroll to and select PROGRAMMED TESTS. 3. Scroll to and select ALL TESTS (or another sequence containing 18 Cl Total-UDV) from TESTING MENU. 4. Scroll to and select 18 Cl Total-UDV from menu. 5. Rinse a clean vial (0156) with sample water. 6. Use the syringe (1184) to add 3mL of sample to the vial. 7. Insert the vial into chamber, close the lid and select SCAN BLANK. 8. Remove the vial from the Spectro.
SMART SPECTRO TEST PROCEDURES 05.
CHROMIUM DIPHENYLCARBOHYDRAZIDE METHOD • CODE 3697-SC QUANTITY 50 1 CONTENTS CODE *Chromium Spectrophotometric Grade Tablets Tablet Crusher 3889A-H 0175 *WARNING: Reagents marked with a * are considered to be potential health hazards. To view or print a Material Safety Data Sheet (MSDS) for these reagents see MSDS CD or www.lamotte.com. To obtain a printed copy, contact LaMotte by e-mail, phone or fax. Chromium is one of a class of heavy metals found in the bottom mud of polluted bodies of water.
PROCEDURE Use universal sample holder 1. Press and hold ON button until spectrophotometer turns on. 2. Scroll to and select PROGRAMMED TESTS. 3. Scroll to and select ALL TESTS (or another sequence containing 23 Chromium-TT) from TESTING MENU. 4. Scroll to and select 23 Chromium-TT from menu. 5. Rinse a tube (0290) with sample water. Fill to 10 mL with sample. 6. Insert the tube into chamber, close lid and select SCAN BLANK. 7. Remove the tube from Spectro. 8.
CHROMIUM-HEXAVALENT DIPHENYLCARBOHYDRAZIDE METHOD • CODE 3645-SC QUANTITY 10 g 1 50 1 CONTENTS *Chromium Reagent Powder Spoon, 0.1 g, plastic Filter Paper Funnel, Plastic CODE *V-6276-D 0699 0465-H 0459 *WARNING: Reagents marked with a * are considered to be potential health hazards. To view or print a Material Safety Data Sheet (MSDS) for these reagents see MSDS CD or www.lamotte.com. To obtain a printed copy, contact LaMotte by e-mail, phone or fax.
PROCEDURE Use universal sample holder. 1. Press and hold ON button until spectrophotometer turns on. 2. Scroll to and select PROGRAMMED TESTS from menu. 3. Scroll to and select ALL TESTS (or another sequence containing 22 Chromium) from TESTING MENU. 4. Scroll to and select 22 Chromium from menu. 5. Rinse a clean tube (0290) with sample water. Fill to the 10 mL line with sample. 6. Insert tube into chamber, close lid and select SCAN BLANK. 7. Remove tube from Spectro. Use the 0.
CHROMIUM-HEXAVALENT, TRIVALENT & TOTAL DIPHENYLCARBOHYDRAZIDE METHOD • CODE 3698-SC QUANTITY 60 mL 10 g 15 mL 30 mL 60 mL 1 1 1 1 1 1 1 1 CONTENTS CODE *Sulfuric Acid, 5N *Chromium Reagent Powder *Sodium Azide, 5% Potassium Permanganate, 0.5% Deionized Water Pipet, plain, glass, w/cap Pipet, 1.0 mL, plastic Spoon, 0.
INTERFERENCES: High concentrations of mercurous and mercuric ions may interfere. HEXAVALENT CHROMIUM PROCEDURE Use universal sample holder. 1. Press and hold ON button until spectrophotometer turns on. 2. Scroll to and select 22 Chromium from menu. 3. Scroll to and select ALL TESTS (or another sequence containing 22 Chromium) from TESTING MENU. 4. Scroll to and select 22 Chromium from menu. 5. Rinse a clean tube (0290) with sample water. Fill to 10 mL line with sample water. 6.
TOTAL CHROMIUM WITH ACID DIGESTION PROCEDURE 1. Fill graduated cylinder (0418) to 50 mL line with sample water. Transfer to Erlenmeyer flask (0431). 2. Use the 1 mL pipet (0354) to add 5 mL (five measures) of *Sulfuric Acid, 3. 4. 5. 6. 7. 8. 9. 10. 11. 12. 13. 14. 15. 16. 17. 5N (7681). Swirl to mix. þ NOTE: Highly buffered waters may require pH adjustment. Adjust the pH of highly buffered samples to 7.0 ±0.5. Continue procedure. Place flask on burner or hot plate. Bring solution to a gentle boil.
TRIVALENT CHROMIUM PROCEDURE Subtract hexavalent chromium from total chromium. Record as ppm trivalent chromium. Trivalent Chromium = Total Chromium–Hexavalent Chromium Chromium-Hexavalent, Trivalent & Total 4/4 SMART SPECTRO TEST PROCEDURES 10.
COBALT PAN METHOD • CODE 4851 QUANTITY CONTENTS CODE 60 mL *Cobalt Buffer *4852H 60 mL *Cobalt Indicator Reagent *4853H 30 mL *Stabilizer Solution *4854G 2 Pipet, 1.0 mL, plastic 0354 1 Pipet, 0.5 mL, plastic 0353 *WARNING: Reagents marked with a * are considered to be potential health hazards. To view or print a Material Safety Data Sheet (MSDS) for these reagents see MSDS CD or www.lamotte.com. To obtain a printed copy, contact LaMotte by e-mail, phone or fax.
PROCEDURE Use universal sample holder. 1. Press and hold ON button until spectrophotometer turns on. 2. Scroll to and select PROGRAMMED TESTS. 3. Scroll to and select ALL TESTS (or another sequence containing 24 Cobalt) from TESTING MENU. 4. Scroll to and select 24 Cobalt from menu. 5. Rinse a tube (0290) with sample water. Fill to 10 mL with sample. 6. Insert the tube into chamber, close lid and select SCAN BLANK. 7. Remove the tube from Spectro. 8. Use the 1.
COD–LOW RANGE MERCURY FREE DIGESTION • Code 0072-SC MERCURY DIGESTION • Code 0075-SC QUANTITY 25 or 25 CONTENTS *COD Low Level Mercury Free Tubes *COD Low Level Mercury Tubes CODE *0072-SC *0075-SC *WARNING: Reagents marked with a * are considered to be potential health hazards. To view or print a Material Safety Data Sheet (MSDS) for these reagents see MSDS CD or www.lamotte.com. To obtain a printed copy, contact LaMotte by e-mail, phone or fax.
Domestic and industrial wastes. 0–150 mg/L COD Dichromate in the presence of silver salts, at high temperature in a closed system, oxidizes most organic compounds to 95-100% of the theoretical amount. This process is called digestion. As dichromate oxidizes the organic compounds, the amount of yellow color is reduced. The remaining yellow color is measured colorimetrically at the 420 nm and is directly proportional to the COD of the sample. SAMPLE Collect samples in glass and test as soon as possible.
PROCEDURE Use universal sample holder 1. Homogenize sample if necessary. 2. Preheat COD heater block to 150±2°C. 3. Remove cap from COD tube vial. Hold vial at a 45° angle. Use a volumetric pipet, to carefully add 2.0 mL sample water allowing the sample to run down the side of the vial. 4. Cap and mix thoroughly. 5. Rinse the outside of the vial with distilled water. Wipe dry with a paper towel. 6. Repeat steps 3 through 5 using 2.0 mL distilled water. This is the reagent blank. 7.
þ NOTES: Reagents are light sensitive. Unused reagents should be stored in the shipping container, and in the refrigerator if possible, until needed. A reagent blank should be run with each set of samples and with each lot of reagents. The reacted blank will be stable if stored in the dark. To eliminate error caused by contamination, wash all glassware with 20% sulfuric acid. For greater accuracy, a minimum of three repetitions should be performed and the results averaged.
COD–STANDARD RANGE MERCURY FREE DIGESTION • CODE 0073-SC MERCURY DIGESTION • CODE 0076-SC QUANTITY 25 or 25 CONTENTS CODE *COD Standard Level Mercury Free Tubes *COD Standard Level Mercury Tubes *0073-SC *0076-SC *WARNING: Reagents marked with a * are considered to be potential health hazards. To view or print a Material Safety Data Sheet (MSDS) for these reagents see MSDS CD or www.lamotte.com. To obtain a printed copy, contact LaMotte by e-mail, phone or fax.
INTERFERENCES: Volatile organic compounds are not oxidized to the extent that they are in the vapor above the digestion solution. Therefore, they do not contribute to the COD reading. Chloride concentrations above 10% of COD interfere with the mercury free tubes. Chloride above 2000 ppm will interfere with the mercury tubes. Nitrite gives a positive interference of 1.1 ppm O2 per ppm NO2–N which is insignificant unless nitrite concentrations are very high.
PROCEDURE Use universal sample holder 1. Homogenize sample if necessary. 2. Preheat COD heater block to 150±2°C. 3. Remove cap from COD tube vial. Hold vial at a 45° angle. Use a volumetric pipet, to carefully add 2.0 mL sample water allowing the sample to run down the side of the vial. 4. Cap and mix thoroughly. 5. Rinse the outside of the vial with distilled water. Wipe dry with a paper towel. 6. Repeat steps 2 through 5 using 2.0 mL distilled water. This is the reagent blank. 7.
þ NOTES: Reagents are light sensitive. Unused reagents should be stored in the shipping container, and in the refrigerator if possible, until needed. A reagent blank should be run with each set of samples and with each lot of reagents. The reacted blank will be stable if stored in the dark. To eliminate error caused by contamination, wash all glassware with 20% sulfuric acid. For greater accuracy, a minimum of three repetitions should be performed and the results averaged.
COD–HIGH RANGE MERCURY FREE DIGESTION • Code 0074-SC MERCURY DIGESTION • Code 0077-SC QUANTITY 25 or 25 CONTENTS *COD High Level Mercury Free Tubes *COD High Level Mercury Tubes CODE *0074-SC *0077-SC *WARNING: Reagents marked with a * are considered to be potential health hazards. To view or print a Material Safety Data Sheet (MSDS) for these reagents see MSDS CD or www.lamotte.com. To obtain a printed copy, contact LaMotte by e-mail, phone or fax.
INTERFERENCES: Volatile organic compounds are not oxidized to the extent that they are in the vapor above the digestion solution. Therefore, they do not contribute to the COD reading. Contains mercury sulfate to prevent interference from chloride. Nitrite gives a positive interference of 1.1 ppm O2 per ppm NO2–N, which is insignificant unless nitrite concentrations are very high. Other reduced inorganic compounds are stoichiometrically oxidized, causing a positive interference.
PROCEDURE Use universal sample holder 1. Homogenize sample if necessary. 2. Preheat COD heater block to 150±2°C. 3. Remove cap from COD tube vial. Hold vial at a 45° angle. Use a graduated pipet, to carefully add 0.2 mL sample water allowing the sample to run down the side of the vial. 4. Cap and mix thoroughly. 5. Rinse the outside of the vial with distilled water. Wipe dry with a paper towel. 6. Repeat steps 3 through 5 using 0.2 mL distilled water. This is the reagent blank. 7.
þ NOTES: Reagents are light sensitive. Unused reagents should be stored in the shipping container, and in the refrigerator if possible, until needed. A reagent blank should be run with each set of samples and with each lot of reagents. The reacted blank will be stable if stored in the dark. To eliminate error caused by contamination, wash all glassware with 20% sulfuric acid. For greater accuracy, a minimum of three repetitions should be performed and the results averaged.
COLOR PLATINUM COBALT METHOD • NO REAGENTS REQUIRED Color in water may be attributed to humus, peat, plankton, vegetation, and natural metallic ions, such as iron and manganese, or industrial waste. Color is removed to make water suitable for domestic and industrial use. Color may have to be removed from industrial waste before it is discharged to a waterway. APPLICATION: Potable water and water with color due to natural materials.
SMART SPECTRO TEST PROCEDURES 05.
COPPER CUPRIZONE METHOD • CODE 4023 QUANTITY 15 mL 15 mL CONTENTS Copper A *Copper B CODE P-6367-E P-6368-E *WARNING: Reagents marked with a * are considered to be potential health hazards. To view or print a Material Safety Data Sheet (MSDS) for these reagents see MSDS CD or www.lamotte.com. To obtain a printed copy, contact LaMotte by e-mail, phone or fax. The copper content of drinking water generally falls below 0.03 parts per million, but copper levels as high as 1.
PROCEDURE Use universal sample holder. 1. Press and hold ON button until spectrophotometer turns on. 2. Scroll to and select PROGRAMMED TESTS. 3. Scroll to and select ALL TESTS (or another sequence containing 31 Cu-Cuprizone) from TESTING MENU. 4. Scroll to and select 31 Cu-Cuprizone from menu. 5. Rinse a tube (0290) with sample water. Fill to the 10 mL line with sample. 6. Insert the tube into chamber, close lid and select SCAN BLANK. 7. Remove tube from Spectro and add 5 drops of Copper A (P-6367).
COPPER–LOW RANGE BICINCHONINIC ACID METHOD • CODE 3640-SC QUANTITY 50 CONTENTS *Copper Tablets CODE T-3808-H *WARNING: Reagents marked with a * are considered to be potential health hazards. To view or print a Material Safety Data Sheet (MSDS) for these reagents see MSDS CD or www.lamotte.com. To obtain a printed copy, contact LaMotte by e-mail, phone or fax. The copper content of drinking water generally falls below 0.03 parts per million, but copper levels as high as 1.
PROCEDURE Use universal sample holder. 1. Press and hold ON button until spectrophotometer turns on. 2. Scroll to and select PROGRAMMED TEST from menu. 3. Scroll to and select ALL TESTS (or another sequence containing 29 Copper BCA-LR) from TESTING MENU. 4. Scroll to and select 29 Copper BCA-LR from menu. 5. Rinse a clean tube (0290) with sample water. Fill to the 10 mL line with sample. 6. Insert tube into chamber, close lid and select SCAN BLANK. 7.
COPPER–HIGH RANGE DIETHYLDITHIOCARBAMATE METHOD • CODE 3646-SC QUANTITY 15 mL CONTENTS *Copper 1 CODE *6446-E *WARNING: Reagents marked with a * are considered to be potential health hazards. To view or print a Material Safety Data Sheet (MSDS) for these reagents see MSDS CD or www.lamotte.com. To obtain a printed copy, contact LaMotte by e-mail, phone or fax. The copper content of drinking water generally falls below 0.03 parts per million, but copper levels as high as 1.
PROCEDURE Use universal sample holder. 1. Press and hold ON button until spectrophotometer turns on. 2. Scroll to and select PROGRAMMED TESTS from menu. 3. Scroll to and select ALL TESTS (or another sequence containing 32 Copper DDC) from TESTING MENU. 4. Scroll to and select 32 Copper DDC from menu. 5. Rinse a clean tube (0290) with sample water. Fill to the 10 mL line with sample. 6. Insert tube into chamber, close lid and select SCAN BLANK. 7. Remove tube from Spectro and add 5 drops of *Copper 1 (6446).
COPPER–UDV BICINCHONINIC ACID METHOD–UNIT DOSE VIALS CODE 4314-H QUANTITY CONTENTS 1 Copper Unit Dose Vials, 10 pouches CODE 4314-H Equipment needed but not suppled: STANDARD ACCESSORY PACKAGE • CODE 1961 1 1 1 Package of 3 Vials (empty) Syringe, 3 mL, plastic Foil Storage Bag 0156 1184 9467 Or: ADVANCED ACCESSORY PACKAGE • CODE 1962 1 1 1 1 1 Pipettor Pipet Tip (0-5 mL) Cuvette Rack Package of 3 Vials (empty) Foil Storage Bag 30528 30695 31695 0156 9467 *WARNING: Reagents marked with a * are co
SAMPLE Copper has a tendency to be adsorbed to the surface of the HANDLING & sample container. Samples should be analyzed as soon as PRESERVATION: possible after collection. If storage is necessary, 0.5 mL of 20% hydrochloric acid per 100 mL of sample will prevent “plating out”. However, a correction must be made to bring the reaction into the optimum pH range. INTERFERENCES: High concentrations of oxidizing agents, calcium, and magnesium interfere. Silver can also interfere.
PROCEDURE Use 10 mm square cell adapter. 1. Press and hold ON button until spectrophotometer turns on. 2. Scroll to and select PROGRAMMED TESTS. 3. Scroll to and select ALL TESTS (or another sequence containing 33 Copper-UDV) from TESTING MENU. 4. Scroll to and select 33 Copper-UDV from menu. 5. Rinse a clean vial (0156) with sample water. 6. Use the syringe (1184) to add 3 mL of sample to the vial. 7. Insert the vial into chamber, close lid and select SCAN BLANK. 8. Remove vial from Spectro. 9.
SMART SPECTRO TEST PROCEDURES 05.
CYANIDE PYRIDINE-BARBITURIC ACID METHOD • CODE 3660-SC QUANTITY 60 mL 5g 5g 15 mL 15 mL 2 1 1 1 CONTENTS Cyanide Buffer *Cyanide Cl Reagent *Cyanide Indicator Reagent *Hydrochloric Acid 1N *Sodium Hydroxide 1N Spoons, 0.1 g, plastic Pipet, plastic, 1.0 mL pH Short Range Test Paper, pH 9–14 Stirring Rod, Plastic CODE 2850PS-H *2794DS-C *2793DS-C *6130-E *4004-E 0699 0354 2955 0519 *WARNING: Reagents marked with a * are considered to be potential health hazards.
PROCEDURE Use universal sample holder. 1. Press and hold ON button until spectrophotometer turns on. 2. Scroll to and select PROGRAMMED TESTS from menu. 3. Scroll to and select ALL TESTS (or another sequence containing 35 Cyanide) from TESTING MENU. 4. Scroll to and select 35 Cyanide from menu. 5. Rinse a clean tube (0290) with sample water. Fill to the 10 mL line with sample. 6.
CYANURIC ACID MELAMINE METHOD–TURBIDITY • CODE 366I-SC QUANTITY 2 x 250 mL 1 CONTENTS *Cyanuric Acid Test Solution Syringe, 5 mL CODE *4856-K 0807 *WARNING: Reagents marked with a * are considered to be potential health hazards. To view or print a Material Safety Data Sheet (MSDS) for these reagents see MSDS CD or www.lamotte.com. To obtain a printed copy, contact LaMotte by e-mail, phone or fax. Cyanuric acid is added to swimming pool water as a stabilizing agent for free chlorine residuals.
PROCEDURE Use universal sample holder. 1. Press and hold ON button until spectrophotometer turns on. 2. Scroll to and select PROGRAMMED TESTS. 3. Scroll to and select ALL TESTS (or another sequence containing 36 Cyanuric) from TESTING MENU. 4. Scroll to and select 36 Cyanuric from menu. 5. Rinse a clean tube (0290) with sample water. Fill to the 10 mL line with sample. 6. Insert tube into chamber, close lid and select SCAN BLANK. 7. Remove tube from Spectro and pour out water.
CYANURIC ACID–UDV MELAMINE METHOD–TURBIDITY–UNIT DOSE VIALS CODE 4313-H QUANTITY CONTENTS 1 Cyanuric Acid Unit Dose Vials, 10 pouches CODE 4313-H Equipment but not suppled: STANDARD ACCESSORY PACKAGE • CODE 1961 1 1 1 Package of 3 Vials (empty) Syringe, 3 mL, plastic Foil Storage Bag 0156 1184 9467 Or: ADVANCED ACCESSORY PACKAGE • CODE 1962 1 1 1 1 1 Pipettor Pipet Tip (0-5 mL) Cuvette Rack Package of 3 Vials (empty) Foil Storage Bag 30528 30695 31695 0156 9467 *WARNING: Reagents marked with a *
PROCEDURE Use 10 mm square cell adapter. 1. Press and hold ON button until spectrophotometer turns on. 2. Scroll to and select PROGRAMMED TESTS. 3. Scroll to and select ALL TESTS (or another sequence containing 37 Cyanuric-UDV) from TESTING MENU. 4. Scroll to and select 37 Cyanuric-UDV from menu. 5. Rinse a clean vial (0156) with sample water. 6. Use the syringe (1184) to add 3 mL of sample to the vial. 7. Insert the vial into chamber, close lid and select SCAN BLANK. 8. Remove vial from Spectro. 9.
DISSOLVED OXYGEN WINKLER COLORIMETRIC METHOD • CODE 3688-SC QUANTITY CONTENTS 30 mL 30 mL 30 mL 1 1 *Manganese Sulfate Solution *Alkaline Potassium Iodide Azide *Sulfuric Acid 1:1 Sample Tube, screw cap Cap CODE *4167-G *7166-G *6141WT-G 29180 28570 *WARNING: Reagents marked with a * are considered to be potential health hazards. To view or print a Material Safety Data Sheet (MSDS) for these reagents see MSDS CD or www.lamotte.com. To obtain a printed copy, contact LaMotte by e-mail, phone or fax.
COLLECTION & TREATMENT OF THE WATER SAMPLE Steps 1 through 4 below describe proper sampling technique in shallow water. For sample collection at depths beyond arm’s reach, special water sampling apparatus is required (e.g. the LaMotte Water Sampling Chamber, Code 1060; Model JT-1 Water Samplers, Code 1077; Water Sampling Outfit, Code 3103; or Water Sampling Bottle, Code 3-0026). 1. To avoid contamination, thoroughly rinse the screw cap Sample Tube (29180) with sample water. 2.
PROCEDURE Use universal sample holder. 1. Press and hold ON button until spectrophotometer turns on. 2. Scroll to and select PROGRAMMED TESTS. 3. Scroll to and select ALL TESTS (or another sequence containing 39 OXYGEN) from TESTING MENU. 4. Scroll to and select 39 OXYGEN from menu. 5. Rinse a clean tube (0290) with untreated sample water. Fill to the 10 mL line with sample. This tube is the BLANK. 6. Insert tube into chamber, close lid and select SCAN BLANK. 7.
SMART SPECTRO TEST PROCEDURES 05.
FLUORIDE SPADNS METHOD • CODE 3647-01-SC QUANTITY 4 x 30 mL 60 mL 1 1 CONTENTS *Acid-Zirconyl-SPADNS Reagent *Sodium Arsenite Solution Pipet, 0.5 mL, plastic Pipet, 1.0 mL, plastic CODE *3875-G *4128-H 0353 0354 *WARNING: Reagents marked with a * are considered to be potential health hazards. To view or print a Material Safety Data Sheet (MSDS) for these reagents see MSDS CD or www.lamotte.com. To obtain a printed copy, contact LaMotte by e-mail, phone or fax.
INTERFERENCES: The following substances produce a positive interference at the concentration given: Chloride (Cl–) 7000 mg/L 16 mg/L Phosphate (PO4–3) 1 mg/L Hexametaphosphate (NaPO3)6 The following substances produce a negative interference at the concentration given: 5000 mg/L Alkalinity (CaCO3) 0.1 mg/L Aluminum (Al3+) 3+ 10 mg/L Iron (Fe ) 200 mg/L Sulfate (SO4–2) Color and turbidity must be removed or compensated for in the procedure. Temperature should be maintained within 5°C of room temperature.
PROCEDURE Use universal sample holder. 1. Press and hold ON button until spectrophotometer turns on. 2. Scroll to and select PROGRAMMED TESTS. 3. Scroll to and select ALL TESTS (or another sequence containing 41 Fluoride) from TESTING MENU. 4. Scroll to and select 41 Fluoride from menu. 5. This test requires a reagent blank. Rinse a clean tube (0290) with clear, colorless, fluoride free water. Fill to the 10 mL line with clear, colorless, fluoride free water. 6. Use the 0.5 mL pipet (0353) to add 0.
SMART SPECTRO TEST PROCEDURES 05.
HYDRAZINE p-DIMETHYLAMINOBENZALDEHYDE METHOD CODE 3656-SC QUANTITY 2x60 mL 10 g 1 1 CONTENTS *Hydrazine Reagent A *Hydrazine Reagent B Powder Pipet, 1.0 mL, plastic Spoon, 0.15 g, plastic CODE *4841-H *4842-D 0354 0727 *WARNING: Reagents marked with a * are considered to be potential health hazards. To view or print a Material Safety Data Sheet (MSDS) for these reagents see MSDS CD or www.lamotte.com. To obtain a printed copy, contact LaMotte by e-mail, phone or fax.
PROCEDURE Use universal sample holder. 1. Press and hold ON button until spectrophotometer turns on. 2. Scroll to and select PROGRAMMED TESTS. 3. Scroll to and select ALL TESTS (or another sequence containing 45 Hydrazine) from TESTING MENU. 4. Scroll to and select 45 Hydrazine from menu. 5. Rinse a clean tube (0290) with sample water. Fill to the 10 mL line with sample. 6. Insert tube into chamber, close lid and select SCAN BLANK. 7. Remove tube from Spectro.
HYDROGEN PEROXIDE–LOW RANGE DPD METHOD • CODE 3662-SC QUANTITY 30 mL 100 1 CONTENTS *Hydrogen Peroxide Reagent #1 *Hydrogen Peroxide LR Tablets Tablet Crusher CODE *6452-G *6454A-J 0175 *WARNING: Reagents marked with a * are considered to be potential health hazards. To view or print a Material Safety Data Sheet (MSDS) for these reagents see MSDS CD or www.lamotte.com. To obtain a printed copy, contact LaMotte by e-mail, phone or fax.
PROCEDURE Use universal sample holder. 1. Press and hold ON button until spectrophotometer turns on. 2. Scroll to and select PROGRAMMED TESTS. 3. Scroll to and select ALL TESTS (or another sequence containing 46 H Peroxide-LR) from TESTING MENU. 4. Scroll to and select 46 H Peroxide-LR from menu. 5. Rinse a clean tube (0290) with sample water. Fill to the 10 mL line with sample. 6. Insert tube into chamber, close lid and select SCAN BLANK. (See Note) 7.
HYDROGEN PEROXIDE– HIGH RANGE DPD Method • CODE 4045 QUANTITY 30 mL 100 1 1 CONTENTS *Hydrogen Peroxide Reagent #1 *Hydrogen Peroxide LR Tablets Tablet Crusher Pipet, glass CODE *6452-G *6454A-J 0175 0342 *WARNING: Reagents marked with a * are considered to be potential health hazards. To view or print a Material Safety Data Sheet (MSDS) for these reagents see MSDS CD or www.lamotte.com. To obtain a printed copy, contact LaMotte by e-mail, phone or fax.
PROCEDURE Use universal sample holder 1. Press and hold ON button until spectrophotometer turns on. 2. Scroll to and select PROGRAMMED TESTS. 3. Select ALL TESTS (or another sequence containing 47 H Per-HR) from TESTING MENU. 4. Scroll to and select 47 H Per-HR from menu. 5. Use the pipet (0342) to add 5 drops of the sample water to a tube (0290). 6. Dilute to the 10 mL line with distilled or hydrogen peroxide-free water. 7. Insert the tube into chamber, close lid and select SCAN BLANK. 8.
HYDROGEN PEROXIDE–SHOCK DPD Method • CODE 4045 QUANTITY 30 mL 100 1 1 CONTENTS *Hydrogen Peroxide Reagent #1 *Hydrogen Peroxide LR Tablets Tablet Crusher Pipet, glass CODE *6452-G *6454A-J 0175 0342 *WARNING: Reagents marked with a * are considered to be potential health hazards. To view or print a Material Safety Data Sheet (MSDS) for these reagents see MSDS CD or www.lamotte.com. To obtain a printed copy, contact LaMotte by e-mail, phone or fax.
PROCEDURE Use universal sample holder 1. Press and hold ON button until spectrophotometer turns on. 2. Scroll to and select PROGRAMMED TESTS. 3. Select ALL TESTS (or another sequence containing 48 H Per Shock) from TESTING MENU. 4. Scroll to and select 48 H Per Shock from menu. 5. Use the pipet (0342) to add 5 drops of the sample water to a tube (0290). 6. Dilute to the 10 mL line with distilled or hydrogen peroxide-free water. 7. Insert the tube into chamber, close lid and select SCAN BLANK. 8.
IRON BIPYRIDYL METHOD • CODE 3648-SC QUANTITY 30 mL 5g 1 1 CONTENTS *Iron Reagent #1 *Iron Reagent #2 Powder Pipet, 0.5 mL, plastic Spoon, 0.1 g, plastic CODE *4450-G *V-4451-C 0353 0699 *WARNING: Reagents marked with a * are considered to be potential health hazards. To view or print a Material Safety Data Sheet (MSDS) for these reagents see MSDS CD or www.lamotte.com. To obtain a printed copy, contact LaMotte by e-mail, phone or fax. Most natural waters contain some iron.
PROCEDURE Use universal sample holder. 1. Press and hold ON button until spectrophotometer turns on. 2. Scroll to and select PROGRAMMED TESTS. 3. Scroll to and select ALL TESTS (or another sequence containing 51 Iron Phen) from TESTING MENU. 4. Scroll to and select 51 Iron Phen from menu. 5. Rinse a clean tube (0290) with sample water. Fill to the 10 mL line with sample. 6. Insert tube into chamber, close lid and select SCAN BLANK. 7. Remove the tube from Spectro.
IRON I,I0-PHENANTHROLINE METHOD • CODE 3668-SC QUANTITY 15 mL 5g 1 CONTENTS CODE *Acid Phenanthroline Indicator *Iron Reducing Reagent Spoon, 0.1 g, plastic *2776-E *2777-C 0699 *WARNING: Reagents marked with a * are considered to be potential health hazards. To view or print a Material Safety Data Sheet (MSDS) for these reagents see MSDS CD or www.lamotte.com. To obtain a printed copy, contact LaMotte by e-mail, phone or fax. Most natural waters contain some iron.
PROCEDURE Use universal sample holder. 1. Press and hold ON button until spectrophotometer turns on. 2. Scroll to and select PROGRAMMED TESTS. 3. Scroll to and select ALL TESTS (or another sequence containing 53 Iron Phen) from TESTING MENU. 4. Scroll to and select 53 Iron Phen from menu. 5. Rinse a clean tube (0290)with sample water. Fill to the 10 mL mark with sample. 6. Insert tube into chamber, close lid and select SCAN BLANK. 7. Remove the tube from Spectro.
IRON–UDV BIPYRIDYL METHOD–UNIT DOSE VIALS • CODE 4315-H QUANTITY 1 CONTENTS *Iron Unit Dose Vials, 50 pouches CODE *4315-H *WARNING: Reagents marked with a * are considered to be potential health hazards. To view or print a Material Safety Data Sheet (MSDS) for these reagents see MSDS CD or www.lamotte.com. To obtain a printed copy, contact LaMotte by e-mail, phone or fax.
INTERFERENCES: Strong oxidizing agents interfere, as well as copper and cobalt in excess of 5.0 ppm. PROCEDURE Use 10 mm square cell adapter. 1. Press and hold ON button. 2. Scroll to and select PROGRAMMED TESTS. 3. Scroll to and select ALL TESTS (or another sequence containing 52 Iron-UDV) from TESTING MENU. 4. Scroll to and select 52 Iron-UDV from menu. 5. Rinse a clean vial (0156) with sample water. 6. Use the syringe (1184) to add 3 mL of sample to the vial. 7.
LEAD PAR METHOD • CODE 4031 QUANTITY 250 mL 15 mL 30 mL 30 mL 15 mL 1 2 CONTENTS Ammonium Chloride Buffer *Sodium Cyanide, 10% PAR Indicator Stabilizing Reagent DDC Reagent Syringe, 5 mL, plastic Pipet, 0.5 mL, plastic CODE 4032-K *6565-E 4033-G 4022-G 4034-E 0807 0353 *WARNING: Reagents marked with a * are considered to be potential health hazards. To view or print a Material Safety Data Sheet (MSDS) for these reagents see MSDS CD or www.lamotte.com.
PROCEDURE Use universal sample holder. 1. Press and hold ON button until spectrophotometer turns on. 2. Scroll to and select PROGRAMMED TESTS. 3. Scroll to and select ALL TESTS (or another sequence containing 54 Lead) from TESTING MENU. 4. Scroll to and select 54 Lead from menu. 5. Rinse a tube (0290) with sample water. Fill to 10 mL with sample. 6. Insert the tube into chamber, close lid and select SCAN BLANK. 7. Remove the tube from Spectro. Use the Syringe (0807) to remove 5mL of sample from tube.
MANGANESE–LOW RANGE PAN METHOD • CODE 3658-01-SC QUANTITY 4 x 30 mL 30 mL 15 mL 1 1 CONTENTS *Hardness Buffer Reagent *Manganese Indicator Reagent *Sodium Cyanide, 10% Pipet, 0.5 mL, plastic Pipet, 1.0 mL, plastic CODE *4255-G *3956-G *6565-E 0369 0354 *WARNING: Reagents marked with a * are considered to be potential health hazards. To view or print a Material Safety Data Sheet (MSDS) for these reagents see MSDS CD or www.lamotte.com. To obtain a printed copy, contact LaMotte by e-mail, phone or fax.
PROCEDURE Use universal sample holder. 1. Press and hold ON button until spectrophotometer turns on. 2. Scroll to and select PROGRAMMED TESTS. 3. Scroll to and select ALL TESTS (or another sequence containing 55 Manganese L) from TESTING MENU. 4. Scroll to and select 55 Manganese L from menu. 5. Rinse a tube (0290) with sample water. Fill to the 10 mL line with sample. 6. Insert tube into chamber, close lid and select SCAN BLANK. 7. Remove tube from Spectro. Use the 1.0 mL pipet (0354) to add 2.
MANGANESE–HIGH RANGE PERIODATE METHOD • CODE 3669-SC QUANTITY 10 g 15 g 1 1 CONTENTS Manganese Buffer Reagent *Manganese Periodate Reagent Spoon, 0.1 g, plastic Spoon, 0.15 g, plastic CODE 6310-D *6311-E 0699 0727 *WARNING: Reagents marked with a * are considered to be potential health hazards. To view or print a Material Safety Data Sheet (MSDS) for these reagents see MSDS CD or www.lamotte.com. To obtain a printed copy, contact LaMotte by e-mail, phone or fax.
PROCEDURE Use universal sample holder. 1. Press and hold ON button until spectrophotometer turns on. 2. Scroll to and select PROGRAMMED TESTS. 3. Scroll to and select ALL TESTS (or another sequence containing 56 Manganese H) from TESTING MENU. 4. Scroll to and select 56 Manganese H from menu. 5. Rinse a tube (0290) with sample water. Fill to the 10 mL line with sample. 6. Insert tube into chamber, close lid and select SCAN BLANK. 7. Remove tube from Spectro. Use the 0.
MERCURY TMK METHOD • CODE 4861 QUANTITY 50 2 x 250 mL 250 mL 1 1 1 1 CONTENTS *TMK Tablets *Propyl Alcohol *Acetate Buffer Tablet Crusher Test Tube, 10 mL, glass, w/cap Pipet, 1.0 mL, plastic Pipet, 0.5 mL, plastic CODE *4862-H *4863-K *4864-K 0175 0778 0354 0353 *WARNING: Reagents marked with a * are considered to be potential health hazards. To view or print a Material Safety Data Sheet (MSDS) for these reagents see MSDS CD or www.lamotte.com.
PREPARATION OF *TMK INDICATOR þ NOTE: Prepare *TMK Indicator daily. Keep out of direct sunlight. Fill test tube (0778) to the 10 mL line with *Propyl Alcohol (4863). Add one *TMK Tablet (4862). Use tablet crusher (0175) to completely crush tablet. Cap and mix. Shake vigorously for 30 seconds. 1. 2. 3. 4. PROCEDURE Use universal sample holder. 1. Press and hold ON button until spectrophotometer turns on. 2. Scroll and select PROGRAMMED TESTS. 3.
MOLYBDENUM THIOGLYCOLATE METHOD • CODE 3699-02-SC QUANTITY 2 x 30 mL 2 x 30 mL 2.5g 1 2 CONTENTS *Mo Buffer *Molybdenum Oxidizing Reagent *Molybdenum Indicator Powder Spoon, 0.05g, plastic Pipets, 1.0 mL, plastic w/cap CODE *3997-G *6485-G *6486-S 0696 0372 *WARNING: Reagents marked with a * are considered to be potential health hazards. To view or print a Material Safety Data Sheet (MSDS) for these reagents see MSDS CD or www.lamotte.com.
PROCEDURE Use universal sample holder. 1. Press and hold ON button until spectrophotometer turns on. 2. Scroll to and select PROGRAMMED TESTS. 3. Scroll to and select ALL TESTS (or another sequence containing 61 Molybdenum-HR) from TESTING MENU. 4. Scroll to and select 61 MOLYBDENUM-HR from menu. 5. Fill clean tube (0290) to 10 mL line with sample water. 6. Insert tube into chamber, close lid and select SCAN BLANK. 7. Remove tube from Spectro. Use a 1.0 mL pipet (0372) to add 1.0 mL of *Mo Buffer (3997).
NICKEL DIMETHYLGLYOXIME METHOD • CODE 3663-SC QUANTITY 60 mL 30 g 30 mL 250 mL 60 mL 60 mL 3 1 1 1 CONTENTS *Hydrochloric Acid, 2.5N *Ammonium Persulfate Reagent *Silver Nitrate Solution, 0.0141N Sodium Citrate, 10% *Dimethylglyoxime, 1% *Ammonium Hydroxide, Conc. Pipets, 1.0 mL, plastic Spoon, 0.1 g, plastic Test tube, 5-10-12.
PROCEDURE Use universal sample holder. 1. Use the 10 mL graduated cylinder (0416) to measure 10 mL of sample water. Pour into glass test tube (0608). 2. Use the 1 mL pipet (0354) to add 1 mL of *Hydrochloric Acid, 2.5N (6251). 3. Use the 0.1 g spoon (0699) to add 2 measures of *Ammonium Persulfate Reagent (6566). Add two drops of *Silver Nitrate Solution, 0.0141N (6346WT). Mix until the powder has dissolved. The solution will be slightly cloudy at this point. 4.
NITRATE ZINC REDUCTION • CODE 3689-SC QUANTITY 50 1 CONTENTS *Nitrate Spectrophotometic Grade Tablets Tablet Crusher CODE *3881A-H 0175 *WARNING: Reagents marked with a * are considered to be potential health hazards. To view or print a Material Safety Data Sheet (MSDS) for these reagents see MSDS CD or www.lamotte.com. To obtain a printed copy, contact LaMotte by e-mail, phone or fax. Nitrogen is essential for plant growth, but excessive amounts in water supplies can result in nutrient pollution.
PROCEDURE Use universal sample holder 1. Press and hold ON button until spectrophotometer turns on. 2. Scroll to and select PROGRAMMED TESTS. 3. Scroll to and select ALL TESTS (or another sequence containing 66 Nitrate-TT) from TESTING MENU. 4. Scroll to and select 66 Nitrate-TT from menu. 5. Rinse a tube (0290) with sample water. Fill to 10 mL with sample. 6. Insert the tube into chamber, close lid and select SCAN BLANK. 7. Remove the tube from Spectro. 8.
NITRATE-NITROGEN–LOW RANGE CADMIUM REDUCTION METHOD • CODE 3649-SC QUANTITY 2 x 60 mL 5g 1 1 CONTENTS *Mixed Acid Reagent *Nitrate Reducing Reagent Spoon, 0.1 g, plastic Dispenser Cap CODE *V-6278-H *V-6279-C 0699 0692 *WARNING: Reagents marked with a * are considered to be potential health hazards. To view or print a Material Safety Data Sheet (MSDS) for these reagents see MSDS CD or www.lamotte.com. To obtain a printed copy, contact LaMotte by e-mail, phone or fax.
Drinking, surface, and saline waters; domestic and industrial waters. RANGE: 0.00–3.00 ppm Nitrate Nitrogen METHOD: Powdered cadmium is used to reduce nitrate to nitrite. The nitrite that is originally present plus reduced nitrate is determined by diazotization of sulfanilamide and nitrite followed by coupling with N-(1 naphthyl)-ethylenediamine dihydrochloride to form a highly colored azo dye which is measured colorimetrically. SAMPLE Analysis should be made as soon as possible.
PROCEDURE Use universal sample holder. þ NOTE: Place Dispenser Cap (0692) on *Mixed Acid Reagent (V-6278). Save this cap for refill reagents. 1. Press and hold ON button until spectrophotometer turns on. 2. Scroll to and select PROGRAMMED TESTS. 3. Scroll to and select ALL TESTS (or another sequence containing 64 Nitrate-N LR) from TESTING MENU. 4. Scroll to and select 64 Nitrate-N LR from menu. 5. Rinse a clean tube (0290) with sample water. Fill to 10 mL line with sample. 6.
SMART SPECTRO TEST PROCEDURES 05.
NITRITE ZINC REDUCTION • CODE 3694-SC QUANTITY 50 1 CONTENTS *Nitrite Spectrophotometric Grade Tablets Tablet Crusher CODE *3886A-H 0175 *WARNING: Reagents marked with a * are considered to be potential health hazards. To view or print a Material Safety Data Sheet (MSDS) for these reagents see MSDS CD or www.lamotte.com. To obtain a printed copy, contact LaMotte by e-mail, phone or fax.
PROCEDURE Use universal sample holder 1. Press and hold ON button until spectrophotometer turns on. 2. Scroll to and select PROGRAMMED TESTS. 3. Scroll to and select ALL TESTS (or another sequence containing 69 Nitrite-TT) from TESTING MENU. 4. Scroll to and select 69 Nitrite-TT from menu . 5. Rinse a tube (0290) with sample water. Fill to 10 mL with sample. 6. Insert the tube into chamber, close lid and select SCAN BLANK. 7. Remove the tube from Spectro. 9.
NITRITE-NITROGEN–LOW RANGE DIAZOTIZATION METHOD • CODE 3650-SC QUANTITY 2 x 60 mL 5g 1 1 CONTENTS *Mixed Acid Reagent *Color Developing Reagent Spoon, 0.1 g, plastic Dispenser Cap CODE *V-6278-H *V-6281-C 0699 0692 *WARNING: Reagents marked with a * are considered to be potential health hazards. To view or print a Material Safety Data Sheet (MSDS) for these reagents see MSDS CD or www.lamotte.com. To obtain a printed copy, contact LaMotte by e-mail, phone or fax.
PROCEDURE Use universal sample holder. þ NOTE: Place Dispenser Cap (0692) on *Mixed Acid Reagent (V-6278). Save this cap for refill reagents. 1. Press and hold ON button until spectrophotometer turns on. 2. Scroll to and select PROGRAMMED TESTS. 3. Scroll to and select ALL TESTS (or another sequence containing 67 Nitrite-N LR) from TESTING MENU. 4. Scroll to and select 67 Nitrite-N LR from menu. 5. Rinse a clean tube (0290) with sample water. Fill to the 10 mL line with sample. 6.
NITROGEN, TOTAL Chromotropic Acid with Persulfate Digestion Method • Code 4026 QUANTITY CONTENTS 25 Total Nitrogen Hydroxide Reagent Tubes 5g 60 mL *Digestion Reagent Powder Deionized Water CODE 4040-G 4036-C *5115PS-H 5g *Total Nitrogen Reagent A Powder *4041-C 30 *Total Nitrogen Reagent B Tablets *4042 25 *Total Nitrogen Acid Reagent Tubes *4043-G 2 Spoon, 0.15 g, plastic 0727 4 Pipets, 1.
Nitrogen is essential for plant growth, but the presence of excessive amounts in water supplies presents a major pollution problem. Nitrogen compounds may enter water as nitrates or be converted to nitrates from agricultural fertilizers, sewage, industrial and packing house wastes, drainage from livestock feeding areas, farm manures and legumes. Nitrates in large amounts can cause “blue babies” (methemoglobinemia) in infants less than six months of age.
PROCEDURE Use universal sample holder 1. Preheat COD reactor to 100 ±2°C. Follow safety precautions. 2. Remove caps from two *Total Nitrogen Hydroxide Reagent Tubes (4040). 3. Use a 0.15 g spoon (0727) and a funnel (0459) to add one level measure of *Digestion Reagent Powder (4036) to each tube. Tap funnel to dispense powder completely. 4. Use a 1.0 mL pipet (0354) to add 2.0 mL of Deionized Water (5115PS), or organic-free water, to one tube. This is the blank. 5. Use another 1.0 mL pipet (0354) to add 2.
23. Wipe the tubes with a damp towel to remove fingerprints and smudges. Wipe with a dry towel. 24. Insert the blank tube into the chamber. Select SCAN BLANK. Remove the blank tube from the spectrophotometer. 25. Insert the sample tube into the chamber. Select SCAN SAMPLE. Record the result as Total Nitrogen in mg/L N. 26. Press OFF button to turn the spectrophotometer off or press EXIT button to exit to a previous menu or make another menu selection.
OXYGEN SCAVENGERS DEHA (Diethylhydroxylamine), Carbohydrazide, Erythorbic Acid, Hydroquinone, Methylethylketoxime IRON REDUCTION METHOD • CODE 4857 QUANTITY CONTENTS CODE 15 mL *DEHA Reagent #1 *4791-E 15 mL *DEHA Reagent #2 *4792-E 15 mL *DEHA Reagent #3 *4793-E *WARNING: Reagents marked with a * are considered to be potential health hazards. To view or print a Material Safety Data Sheet (MSDS) for these reagents see MSDS CD or www.lamotte.com.
DEHA PROCEDURE Use universal sample holder 1. Press and hold ON button until spectrophotometer turns on. 2. Scroll to and select PROGRAMMED TESTS. 3. Scroll to and select ALL TESTS (or another sequence containing 38 DEHA) from TESTING MENU. 4. Scroll to and select 38 DEHA from menu. 5. Rinse a tube (0290) with sample water. Fill to 10 mL with sample. 6. Insert the tube into chamber, close lid and select SCAN BLANK. 7. Remove the tube from spectrophotometer. 8. Add 3 drops of *DEHA Reagent #1 (4791).
CARBOHYDRAZIDE PROCEDURE Use universal sample holder 1. Press and hold ON button until spectrophotometer turns on. 2. Scroll to and select PROGRAMMED TESTS. 3. Scroll to and select ALL TESTS (or another sequence containing 14 c-hydrazide) from TESTING MENU. 4. Scroll to and select 14 c-hydrazide from menu. 5. Rinse a tube (0290) with sample water. Fill to 10 mL with sample. 6. Insert the tube into chamber, close lid and select SCAN BLANK. 7. Remove the tube from spectrophotometer. 8.
ERYTHORBIC ACID PROCEDURE Use universal sample holder 1. Press and hold ON button until spectrophotometer turns on. 2. Scroll to and select PROGRAMMED TESTS. 3. Scroll to and select ALL TESTS (or another sequence containing 40 E-thorbic A) from TESTING MENU. 4. Scroll to and select 40 E-thorbic A from menu. 5. Rinse a tube (0290) with sample water. Fill to 10 mL with sample. 6. Insert the tube into chamber, close lid and select SCAN BLANK. 7. Remove the tube from spectrophotometer. 8.
HYDROQUINONE PROCEDURE Use universal sample holder 1. Press and hold ON button until spectrophotometer turns on. 2. Scroll to and select PROGRAMMED TESTS. 3. Scroll to and select ALL TESTS (or another sequence containing 49 H-quinone) from TESTING MENU. 4. Scroll to and select 49 H-quinone from menu. 5. Rinse a tube (0290) with sample water. Fill to 10 mL with sample. 6. Insert the tube into chamber, close lid and select SCAN BLANK. 7. Remove the tube from spectrophotometer. 8.
METHYLETHYLKETOXIME PROCEDURE Use universal sample holder 1. Press and hold ON button until spectrophotometer turns on. 2. Scroll to and select PROGRAMMED TESTS. 3. Scroll to and select ALL TESTS (or another sequence containing 58 m-e-ketoxim) from TESTING MENU. 4. Scroll to and select 58 m-e-ketoxim from menu. 5. Rinse a tube (0290) with sample water. Fill to 10 mL with sample. 6. Insert the tube into chamber, close lid and select SCAN BLANK. 7. Remove the tube from spectrophotometer. 8.
OZONE INDIGO METHOD • CODE 365I-SC QUANTITY 15 mL 250 mL 30 mL 1 1 1 1 1 1 CONTENTS Chlorine Inhibitor *Ozone Buffer Indigo Blue Stock Solution Sampling Apparatus Pipet, transfer, 1.0 mL Pipet, transfer, 5 mL Pump, 10 mL Bottle, HR Reagent, amber glass Graduated Cylinder, 50 mL, glass CODE 3990-E *3991-K 3989-G 0681 2-2170 0329 30527 0680-J 0418 WARNING: Reagents marked with a * are considered to be potential health hazards.
PROCEDURE–LOW RANGE Use universal sample holder. A. PREPARATION OF HR REAGENT þ NOTE: The quantity of Indigo Blue Stock solution (3989) supplied will prepare one batch of HR Reagent for the High Range Ozone procedure or five batches of HR Reagent for the Low Range Ozone procedure. 1. Use the 50 mL graduated cylinder to carefully add 45 mL of *Ozone Buffer (3991) to amber glass bottle marked HR Reagent (0680). 2.
PROCEDURE–HIGH RANGE Use universal sample holder. A. PREPARATION OF HR REAGENT þ NOTE: The quantity of Indigo Blue Stock solution (3989) supplied will prepare one batch of HR Reagent for the High Range Ozone procedure or five batches of HR Reagent for the Low Range Ozone procedure. 1. Use the 50 mL graduated cylinder to carefully add 35 mL of *Ozone Buffer (3991) to amber glass bottle marked HR Reagent (0680). 2.
SMART SPECTRO TEST PROCEDURES 07.
pH COLORIMETRIC METHOD • CODE 3700-SC QUANTITY CONTENTS 60 mL 60 mL 60 mL 3 Chlorphenol Red Indicator Phenol Red Indicator Thymol Blue Indicator Pipets, 0.5 mL, plastic w/caps CODE V-2209-H V-2304-H V-2213-H 0369 The term pH (always written with a lower case p and an upper case H) is correctly defined as the negative logarithm of the hydrogen ion concentration.
APPLICATION: METHOD: Drinking, surface, and saline waters; swimming pool water; domestic and industrial wastes. The various pH indicators exhibit a specific color change over a narrow pH range. The color changes are measured colorimetrically. Sample should be analyzed immediately after collection. SAMPLE HANDLING & PRESERVATION: INTERFERENCES: Sample color and turbidity interfere with the colorimetric pH measurement.
PHENOL AMINOANTIPYRINE METHOD • CODE 3652-SC QUANTITY 5g 30 mL 2 x 60 mL 1 1 1 CONTENTS Aminoantipyrine Reagent *Ammonium Hydroxide Solution *Potassium Ferricyanide Solution Spoon, 0.1 g, plastic Pipet, plain, plastic Pipet, 1.0 mL, plastic CODE 7825-C *7826-G *7827-H 0699 0352 0354 *WARNING: Reagents marked with a * are considered to be potential health hazards. To view or print a Material Safety Data Sheet (MSDS) for these reagents see MSDS CD or www.lamotte.com.
PROCEDURE Use universal sample holder. 1. Press and hold ON button until spectrophotometer turns on. 2. Scroll to and select PROGRAMMED TESTS. 3. Scroll to and select ALL TESTS (or another sequence containing 77 Phenol) from TESTING MENU. 4. Scroll to and select 77 Phenol from menu. 5. Rinse a clean tube (0290) with sample water. Fill to the 10 line with sample. 6. Insert tube into chamber, close lid and select SCAN BLANK. 7. Remove tube from Spectro. Use the 0.
PHOSPHATE–LOW RANGE ASCORBIC ACID REDUCTION METHOD • CODE 3653-SC QUANTITY 60 mL 5g 1 1 CONTENTS *Phosphate Acid Reagent *Phosphate Reducing Reagent Pipet, 1 mL, plastic Spoon, 0.1 g, plastic CODE *V-6282-H *V-6283-C 0354 0699 *WARNING: Reagents marked with a * are considered to be potential health hazards. To view or print a Material Safety Data Sheet (MSDS) for these reagents see MSDS CD or www.lamotte.com. To obtain a printed copy, contact LaMotte by e-mail, phone or fax.
Drinking, surface, and saline waters; domestic and industrial wastes (Method based on reactions that are specific for orthophosphate). RANGE: 0.00–3.00 ppm Orthophosphate METHOD: Ammonium molybdate and antimony potassium tartrate react in a filtered acid medium with dilute solution of PO4–3 to form an antimony-phosphomolybdate complex. This complex is reduced to an intense blue colored complex by ascorbic acid. The color is proportional to the amount of phosphate present.
PROCEDURE Use universal sample holder. 1. Press and hold ON button until spectrophotometer turns on. 2. Scroll to and select PROGRAMMED TESTS. 3. Scroll to and select ALL TESTS (or another sequence containing 78 Phosphate-L) from TESTING MENU. 4. Scroll to and select 78 Phosphate-L from menu. 5. Rinse a clean tube (0290) with sample water. Fill to the 10 line with sample. 6. Insert tube into chamber, close lid and select SCAN BLANK. 7. Remove tube from Spectro. Use 1.0 mL pipet (0354) to add 1.
SMART SPECTRO TEST PROCEDURES 05.
PHOSPHATE–HIGH RANGE VANADOMOLYBDOPHOSPHORIC ACID METHOD CODE 3655-SC QUANTITY 2 x 60 mL 1 CONTENTS *VM Phosphate Reagent Pipet, 1.0 mL, plastic CODE *4410-H 0354 *WARNING: Reagents marked with a * are considered to be potential health hazards. To view or print a Material Safety Data Sheet (MSDS) for these reagents see MSDS CD or www.lamotte.com. To obtain a printed copy, contact LaMotte by e-mail, phone or fax.
PROCEDURE Use universal sample holder. 1. Press and hold ON button until spectrophotometer turns on. 2. Scroll to and select PROGRAMMED TESTS. 3. Scroll to and select ALL TESTS (or another sequence containing 79 Phosphate-H) from TESTING MENU. 4. Scroll to and select 79 Phosphate-H from menu. 5. Rinse a clean tube (0290) with sample water. Fill to the 10 mL line with sample. 6. Insert tube into chamber, close lid and select SCAN BLANK. 7. Remove tube from Spectro. Use the 1.0 mL pipet (0354) to add 2.
PHOSPHORUS, TOTAL - LOW RANGE Ascorbic Acid Reduction with Persulfate Digestion Method Code 4024 QUANTITY 25 5g 2 X 30 mL 2 X 30 mL 5g 1 3 1 2 CONTENTS *Total Phosphorus Acid Reagent Tubes *Digestion Reagent Powder *Total Phosphorus LR Hydroxide Reagent *Phosphate Acid Reagent Phosphate Reducing Reagent Spoon, 0.15 g, plastic Pipets, 1.0 mL, plastic Spoon, 0.
Phosphorus in natural waters and wastewaters occurs almost exclusively in the form of orthophosphates, condensed phosphates (pyro-, meta- and other polyphosphates) and organically bound phosphates. Phosphates may be added in small amounts to water supplies during treatment. Larger amounts are introduced to water used for cleaning or laundering as components of commercial cleaning preparations. Phosphates are used to treat boiler water and are components of agricultural and residential fertilizers.
PROCEDURE Use universal sample holder 1. Preheat COD reactor to 150 ±2°C. Follow safety precautions. 2. Remove cap from a *Total Phosphorus Acid Reagent Tube (4035). Use a 1.0 mL pipet (0354) to add 5.0 mL of sample. 3. Use the 0.15 g spoon (0727) and a funnel (0459) to add one level measure of *Digestion Reagent Powder (4036). Tap funnel to dispense powder completely. Cap tube tightly and shake until powder dissolves completely. 4. Place the tube in the COD reactor for 30 minutes. 5.
SMART SPECTRO TEST PROCEDURES 05.
PHOSPHORUS, TOTAL - HIGH RANGE Molybdovanadate Method with Acid Persulfate Digestion Code 4025 QUANTITY 25 60 mL 5g 2 X 30 mL 30 mL 1 3 1 1 CONTENTS *Total Phosphorus Acid Reagent Tubes Deionized Water *Digestion Reagent Powder *Total Phosphorus HR Hydroxide Reagent *Total Phosphorus HR Indicator Reagent Spoon, 0.15 g Pipets 1.0 mL, plastic Pipet, 0.
generally not more than 0.1 mg/L unless the water has become polluted from wastewater sources or excessive drainage from agricultural areas. APPLICATION: Boiler, cooling, and industrial water. RANGE: 0.0–100.0 mg/L Total Phosphorus as phosphate METHOD: Pretreatment of the sample with heat and acid provides conditions for the hydrolysis of condensed inorganic phosphates. Heat, acid and persulfate convert the organic phosphates to orthophosphate during the digestion.
PROCEDURE Use universal sample holder 1. Preheat COD reactor to 150 ±2°C. Follow safety precautions. 2. Remove cap from a *Total Phosphorus Acid Reagent Tube (4035). Use a 1.0 mL pipet (0354) to add 5.0 mL of Deionized Water (5115PS). This is the blank. 3. Remove cap from a *Total Phosphorus Acid Reagent Tube (4035). Use another 1.0 mL pipet (0354) to add 5.0 mL of sample water. This is the sample. 4. Use the 0.
SMART SPECTRO TEST PROCEDURES 05.
POTASSIUM TETRAPHENYLBORON METHOD • CODE 3639-SC QUANTITY 30 mL 5g 1 CONTENTS *Sodium Hydroxide, 1.0N *Tetraphenylboron Powder Spoon, 0.05 g, plastic CODE *4004WT-G *6364-C 0696 *WARNING: Reagents marked with a * are considered to be potential health hazards. To view or print a Material Safety Data Sheet (MSDS) for these reagents see MSDS CD or www.lamotte.com. To obtain a printed copy, contact LaMotte by e-mail, phone or fax.
PROCEDURE Use universal sample holder. 1. Press and hold ON button until spectrophotometer turns on. 2. Scroll to and select PROGRAMMED TESTS. 3. Scroll to and select ALL TESTS (or another sequence containing 81 Potassium) from TESTING MENU. 4. Scroll to and select 81 Potassium from menu. 5. Rinse a clean tube (0290) with sample water. Fill to the 10 mL line with sample. 6. Insert tube into chamber, close lid and select SCAN BLANK. 7. Remove tube from Spectro. Add 4 drops of *Sodium Hydroxide, 1.
SILICA–LOW RANGE HETEROPOLY BLUE METHOD • CODE 3664-SC QUANTITY 30 mL 30 mL 30 mL 10 g 1 CONTENTS *Silica Reagent #1 *Silica Reagent #2 *Silica Reagent #3 *Silica Reagent #4 Spoon, 0.1 g, plastic CODE *V-4466-G *V-4467-G *V-4468-G *V-6284-D 0699 *WARNING: Reagents marked with a * are considered to be potential health hazards. To view or print a Material Safety Data Sheet (MSDS) for these reagents see MSDS CD or www.lamotte.com. To obtain a printed copy, contact LaMotte by e-mail, phone or fax.
Drinking, surface, and saline waters; domestic and industrial wastes. RANGE: 0.00–2.50 ppm Silica METHOD: Reactive silica forms a complex with ammonium molybdate in an acidic solution to produce a yellow-green color in proportion to the amount of silica present. Phosphate also reacts with molybdate but the addition of oxalic acid eliminates the molybdophosphoric acid complex. This silica molybdate complex is then reduced by ascorbic acid to produce an intense blue color.
PROCEDURE Use universal sample holder. 1. Press and hold ON button until spectrophotometer turns on. 2. Scroll to and select PROGRAMMED TESTS. 3. Scroll to and select ALL TESTS (or another sequence containing 85 Silica Lo) from TESTING MENU. 4. Scroll to and select 85 Silica Lo from menu. 5. Rinse a clean tube (0290) with sample water. Fill to the 10 mL line with sample. 6. Insert tube into chamber, close lid and select SCAN BLANK. (See Note) 7. Remove tube from Spectro.
SMART SPECTRO TEST PROCEDURES 05.
SILICA–HIGH RANGE SILICOMOLYBDATE METHOD • CODE 3687-SC QUANTITY 30 mL 30 mL 15 mL CONTENTS *Silica Reagent #1 *Silica Reagent #2 *Silica Reagent #3 CODE *V-4466-G *V-4467-G *V-4468-G *WARNING: Reagents marked with a * are considered to be potential health hazards. To view or print a Material Safety Data Sheet (MSDS) for these reagents see MSDS CD or www.lamotte.com. To obtain a printed copy, contact LaMotte by e-mail, phone or fax.
PROCEDURE Use universal sample holder. 1. Press and hold ON button until spectrophotometer turns on. 2. Scroll to and select PROGRAMMED TESTS. 3. Scroll to and select ALL TESTS (or another sequence containing 86 Silica Hi) from TESTING MENU. 4. Scroll to and select 86 Silica Hi from menu. 5. Rinse a clean tube (0290) with sample water. Fill to the 10 mL line with sample. 6. Insert tube into chamber, close lid and select SCAN BLANK. 7. Remove tube from Spectro. Add 6 drops *Silica Reagent #1 (V-4466).
SULFATE BARIUM CHLORIDE METHOD • CODE 3665-SC QUANTITY 10 g 1 CONTENTS *Sulfate Reagent Spoon, 0.1 g, plastic CODE *V-6277-D 0699 *WARNING: Reagents marked with a * are considered to be potential health hazards. To view or print a Material Safety Data Sheet (MSDS) for these reagents see MSDS CD or www.lamotte.com. To obtain a printed copy, contact LaMotte by e-mail, phone or fax.
PROCEDURE Use universal sample holder. 1. Press and hold ON button until spectrophotometer turns on. 2. Scroll to and select PROGRAMMED TESTS. 3. Scroll to and select ALL TESTS (or another sequence containing 89 SULFATE-HR) from TESTING MENU. 4. Scroll to and select 89 SULFATE-HR from menu. 5. Rinse a clean tube (0290) with sample water. Fill to the 10 mL line with sample. 6. Insert tube into chamber, close lid and select SCAN BLANK. 7. Remove tube from Spectro. Use the 0.
SULFIDE METHYLENE BLUE METHOD • CODE 3654-01-SC QUANTITY 2 X 30 mL 15 mL 2 x 60 mL 2 CONTENTS *Sulfide Reagent A *Sulfide Reagent B Sulfide Reagent C Pipets, 1.0 mL, plastic CODE *V-4458-G *V-4459-E 4460-H 0354 *WARNING: Reagents marked with a * are considered to be potential health hazards. To view or print a Material Safety Data Sheet (MSDS) for these reagents see MSDS CD or www.lamotte.com. To obtain a printed copy, contact LaMotte by e-mail, phone or fax.
PROCEDURE Use universal sample holder. 1. Press and hold ON button until spectrophotometer turns on. 2. Scroll to and select PROGRAMMED TESTS. 3. Scroll to and select ALL TESTS (or another sequence containing 90 Sulfide-LR) from TESTING MENU. 4. Scroll to and select 90 Sulfide-LR from menu. 5. Rinse a clean tube (0290) with sample water. Fill to the 10 mL line with sample. 6. Insert tube into chamber, close lid and select SCAN BLANK. 7. Remove tube from Spectro. Use the 1.0 mL pipet (0354) to add 1.
SURFACTANTS ION PAIR EXTRACTION–BROMPHENOL BLUE INDICATOR CODE 4876 QUANTITY 50 g 10 g 2 X 60 mL 1 1 1 CONTENTS pH Adjustment Powder Sodium Chloride Reagent *DS Indicator Reagent Spoon, 0.5 g, plastic Spoon, 0.1 g, plastic Pipet, 1.0 mL, plastic CODE 4509- H 4877-D *4508-H 0698 0699 0354 *WARNING: Reagents marked with a * are considered to be potential health hazards. To view or print a Material Safety Data Sheet (MSDS) for these reagents see MSDS CD or www.lamotte.com.
PROCEDURE Use universal sample holder. 1. Press and hold ON button until spectrophotometer turns on. 2. Scroll to and select PROGRAMMED TESTS. 3. Scroll to and select ALL TESTS (or another sequence containing 94 Surfactants) from TESTING MENU. 4. Scroll to and select 94 Surfactants from menu. 5. Rinse a tube (0290) with sample water. Fill to 10 mL line with sample. 6. Insert the tube into chamber, close lid and select SCAN BLANK. 7. Remove the tube from Spectro. 8. Use the 0.5 g spoon (0698) to add 0.
TANNIN TUNGSTO-MOLYBDOPHOSPHORIC ACID METHOD CODE 3666-SC QUANTITY 30 mL 2 x 60 mL 1 1 CONTENTS *Tannin Reagent #1 *Tannin Reagent #2 Pipet, plain, plastic Pipet, 1.0 mL, plastic CODE *7833-G *7834-H 0352 0354 *WARNING: Reagents marked with a * are considered to be potential health hazards. To view or print a Material Safety Data Sheet (MSDS) for these reagents see MSDS CD or www.lamotte.com. To obtain a printed copy, contact LaMotte by e-mail, phone or fax.
PROCEDURE Use universal sample holder. 1. Press and hold ON button until spectrophotometer turns on. 2. Scroll to and select PROGRAMMED TESTS. 3. Scroll to and select ALL TESTS (or another sequence containing 96 TANNIN) from TESTING MENU. 4. Scroll to and select 96 TANNIN from menu. 5. Rinse a clean tube (0290) with sample water. Fill to the 10 mL line with sample. 6. Insert tube into chamber, close lid and select SCAN BLANK. 7. Remove tube from Spectro.
TURBIDITY ABSORPTION METHOD • NO REAGENTS REQUIRED Turbidity is a measure of water clarity and is independent of color. Turbidity is caused by undissolved and suspended solids. Mud, silt, algae, and microorganisms can all cause turbidity. Turbidity is a gross measurement of water quality. APPLICATION: Surface and industrial waters for non-compliance monitoring. (For compliance monitoring at low turbidity levels, use a commercial .
PROCEDURE Use universal sample holder. 1. Press and hold ON button until spectrophotometer turns on. 2. Scroll to and select PROGRAMMED TESTS. 3. Scroll to and select ALL TESTS (or another sequence containing 98 Turbidity) from TESTING MENU. 4. Scroll to and select 98 Turbidity from menu. 5. Rinse a clean tube (0290) with deionized water (turbidity free). Fill to the 10 mL line with deionized water. 6. Insert tube into chamber, close lid and select SCAN BLANK. 7.
ZINC ZINCON METHOD • CODE 3667-SC QUANTITY 30 mL 120 mL 10 g 25 g 15 mL 30 mL 1 1 1 2 1 CONTENTS *Zinc Indicator Solution *Methyl Alcohol Sodium Ascorbate Powder *Zinc Buffer Powder *Sodium Cyanide, 10% *Formaldehyde Solution, 37% “Dilute Zinc Indicator Solution” Bottle, with 1 mL pipet assembly Graduated Cylinder, 10 mL, glass Spoon, 0.5 g, plastic Pipets, plain, plastic Spoon, 0.
Drinking and surface waters; domestic and industrial wastewaters. RANGE: 0.00–3.00 ppm Zinc METHOD: Zinc forms a blue colored complex with Zincon in a solution buffered at pH 9.0. Other heavy metals are complexed by cyanide and the zinc cyanide complex is released by the addition of formaldehyde before the other metal cyanide complexes are destroyed. Sodium ascorbate is added to reduce the interference of manganese. SAMPLE Sample should be analyzed within 6 hours after collection.
PROCEDURE Use universal sample holder. A. PREPARATION OF DILUTE ZINC INDICATOR SOLUTION 1. Use a pipet (0352) to measure exactly 5.0 mL of *Zinc Indicator Solution (6314) into 10 mL graduated cylinder (0416). The bottom of the curved surface (the meniscus) of liquid should be at 5.0 mL mark. Pour this into the bottle labeled “Dilute Zinc Indicator Solution”. 2. Use unrinsed graduated cylinder to add 10.0 mL and then 7.8 mL (total of 17.
SMART SPECTRO TEST PROCEDURES 05.
SMARTSpectro ••••••••••••••••••••••••••••••••••••••••••••••• Spectrophotometer APPENDIX L Mott
SMART SPECTRO TEST PROCEDURES 05.
APPENDIX Ammonia in water occurs in two forms: toxic unionized ammonia (NH3) and the relatively non-toxic ionized form, ammonium ion (NH4+). This test method measures both forms as ammonia-nitrogen (NH3–N) to give the total ammonia-nitrogen concentration in water. The actual proportion of each compound depends on temperature, salinity, and pH. A greater concentration of unionized ammonia is present when the pH value and salinity increase. 1.
FOR EXAMPLE: If a fresh water sample at 20°C has a pH of 8.5 and the test result is 1.0 ppm as Total Ammonia-Nitrogen: 1. The percentage from the table is 11.18% (or 0.1118). 2. 1 ppm Total Ammonia-Nitrogen x 0.1118 = 0.1118 ppm Unionized Ammonia-Nitrogen. 3. Total Ammonia-Nitrogen 1.0000 ppm Unionized Ammonia-Nitrogen - 0.1118 ppm Ionized Ammonia-Nitrogen Appendix 2/2 = 0.8882 ppm SMART SPECTRO TEST PROCEDURES 05.
