OPERATOR’S MANUAL v3.0 and higher • Printed 2.
CONTENTS GENERAL INFORMATION Packaging & Delivery .................................................................................... General Precautions ...................................................................................... Safety Precautions ........................................................................................ Limits of Liability ............................................................................................ Warranty ......................................
CALIBRATION Calibrate Wavelength .................................................................................. 24 PROGRAMMED TESTS Introduction ................................................................................................. Sequences of Tests ..................................................................................... General Testing Procedures ........................................................................ Testing with the Programmed Tests .....................
GENERAL INFORMATION PACKAGING & DELIVERY Experienced packaging personnel at LaMotte Company assure adequate protection against normal hazards encountered in transportation of shipments. After the product leaves the manufacturer, all responsibility for its safe delivery is assured by the transportation company. Damage claims must be filed immediately with the transportation company to receive compensation for damaged goods.
LIMITS OF LIABILITY Under no circumstances shall LaMotte Company be liable for loss of life, property, profits, or other damages incurred through the use or misuse of their products. WARRANTY LaMotte Company warrants this instrument to be free of defects in parts and workmanship for 2 years from the date of shipment.
SPECIFICATIONS INSTRUMENT TYPE: Single beam spectrophotometer Readout 5 line, 18 character per line LCD Wavelengths 350-1000 nm Wavelength Accuracy ± 2 nm Wavelenth Resolution 1 nm Wavelength Bandwidth 5 nm (max) Photometric Range 0-125%T, –0.1-2.5A Photometric Accuracy ± 0.005A Photometric Stray Light <0.
Precision: Precision is the numerical agreement between two or more measurements.3 The precision can be reported as a range for a measurement (difference between the min and max). It can also be reported as the standard deviation or the relative standard deviation. It is a measure of how close together the measurements are, not how close they are to the correct or true value. The precision can be very good and the accuracy very bad. This is a useful measure of the performance of a test method. 3.Skoog, D.A.
Reproducibility: Reproducibility is the between-run precision.7 7.Jeffery G. H., Basset J., Mendham J., Denney R. C., Vogel’s Textbook of Quantitative Chemical Analysis, 5th ed., Longman Scientific & Technical, 1989, p. 130.
CHEMICAL TESTING WATER SAMPLING FOR CHEMICAL ANALYSIS Taking Representative Samples The underlying factor to be considered for any type of water sampling is whether or not the sample is truly representative of the source. To properly collect a representative sample: • Sample as frequently as possible. • Collect a large sample or at least enough to conduct whatever tests are necessary. • Make a composite sample for the same sampling area.
Sampler (LaMotte Code 1060) will serve as a sub-surface or in-depth sampler. This weighted device is lowered to the sampling depth and allowed to rest at this depth for a few minutes. The water percolates into the sample chamber displacing the air which bubbles to the surface. When the bubbles cease to rise, the device has flushed itself approximately five times and it may be raised to the surface for examination.
AN INTRODUCTION TO COLORIMETRIC ANALYSIS & SPECTROSCOPY Most test substances in water are colorless and undetectable to the human eye. To test for their presence we must find a way to “see” them. The LaMotte SMART Spectro can be used to measure any test substance that is itself colored or can be reacted to produce a color. In fact a simple definition of colorimetry is “the measurement of color” and a colorimetric method is “any technique used to evaluate an unknown color in reference to known colors”.
The choice of the correct wavelength for testing is important. It is interesting to note that the wavelength that gives the most sensitivity (lower detection limit) for a test factor is the complementary color of the test sample. For example the Nitrate-Nitrogen test produces a pink color proportional to the nitrate concentration in the sample (the greater the nitrate concentration, the darker the pink color).
Below is a list of suggested wavelength ranges for the color of the reacted samples. Use these as a starting point. Sample Color Wavelength Range Yellow 350-450 Yellow-Orange 450-490 Orange 490-510 Pink 510-570 Red 570-600 Green and Blue 600-750 CALIBRATION CURVES The SMART Spectro contains precalibrated tests for the LaMotte reagent systems. The first step in using a non-LaMotte reagent system with the SMART Spectro is to create a calibration curve for the reagent system.
5. Plot results on graph paper or computer using any available plotting program. If results are as %T versus concentration, semilog graph paper must be used. Plot the standard solution concentrations on the horizontal, linear axis, and the %T on the vertical, logarithmic axis. If results are as absorbance versus standard solution concentration, simple linear graph paper can be used. Plot the standard solution concentration on the horizontal axis, and the absorbance on the vertical axis. 6.
PREPARING DILUTE STANDARD SOLUTIONS Standard solutions should be prepared to create a calibration curve. Standard solutions can be prepared by diluting a known concentrated standard by specified amounts. A chart or computer spreadsheet can be created to determine the proper dilutions. Use volumetric flasks and volumetric pipets for all dilutions. 1. In Column A – Record the maximum concentration of test as determined by the range and path length. 2.
equal the concentration of the sample plus that added with the “spike”, then an interference may exist. For example, a 10.0 mL water sample was determined to contain 0.3 ppm iron. To a second 10.0 mL sample, 0.1 mL of 50 ppm iron standard was added. The concentration of iron due to the “spike” was (0.10 mL x 50 ppm)/10.0 mL = 0.50 ppm. The concentration of iron determined in the spiked sample should be 0.3 + 0.5 = 0.8 ppm iron. (Note: any error due to the increased volume from the “spike” is negligible).
which is in the concentration range for the test, is obtained. Be sure to multiply the concentration found by the dilution factor (the number of total 10 mL volumes used). Example: 10 mL of sample is diluted with three 10 mL volumes of demineralized water; the dilution factor is four. INTERFERENCES LaMotte reagent systems are designed to minimize most common interferences. Each individual test instruction discusses interferences unique to that test.
OPERATION OF THE SMART SPECTRO OVERVIEW The SMART Spectro is a portable, microprocessor controlled, direct reading, single beam spectrophotometer. It has a 5 line, 18 character liquid crystal display for alphabetical and numerical messages. The operation is controlled with the keypad through menu driven software in response to selections shown on the display. The test library consists of over 80 LaMotte tests and 25 “User Tests”.
COMPONENTS Figure 1 shows a diagram of the SMART Spectro and the components. RS232 Serial Port AC Adapter Socket 10mm Square Cell in Adapter 25mm Round Cell in Universal Adapter Display Chamber Scroll Between Choices Turn Off the Spectro Make Selections Exit Menus Scroll Between Choices Turn On the Spectro Figure 1 SMART Spectro Operator’s Manual 2.
QUICK START 1. Press ON. The LaMotte logo screen will appear for about 2 seconds and the MAIN MENU will appear. MAIN MENU 12:00:01 * CALIBRATE WL PROGRAMMED TESTS %T/ABS PC LINK MAIN MENU 12:00:01 2. Press or to scroll to PROGRAMMED TESTS. CALIBRATE WL * PROGRAMMED TESTS %T/ABS PC LINK 3. Press *ENTER to select PROGRAMMED TESTS. PROGRAMMED TESTS * SEQUENCE 1 SEQUENCE 2 SEQUENCE 3 ALL TESTS 4. Press TESTS. PROGRAMMED TESTS to scoll to ALL SEQUENCE 1 SEQUENCE 2 SEQUENCE 3 * 5.
6. PresS or desired test. ALL TESTS to scroll to the 1 Alkalinity-UDV * 2 Aluminum 3 Ammonia-N L F 4 Ammonia-N L s 7. Press *ENTER to select the test. 2 Aluminum * SCAN BLANK SCAN SAMPLE END 8. Insert the blank. Press *ENTER to scan the blank. 2 Aluminum SCAN BLANK * 9. Insert the reacted sample. Press *ENTER to scan the sample. The result will be displayed. 535 NM SCAN SAMPLE END 535 NM 2 Aluminum T = 16.5%T A = 0.7834A C = 1.
GENERAL OPERATING PROCEDURES The operation of the SMART Spectro is controlled by a microprocessor. The microprocessor is programmed with menu driven software. A menu is a list of choices. This allows a selection of various tasks for the spectrophotometer to perform, such as, scan blank, scan sample, and edit test sequences. The keypad is used to make menu selections which are viewed in the display.
THE DISPLAY & THE MENUS The display allows menu selections to be viewed and chosen. These choices instruct the spectrophotometer to perform specific tasks. The menus are viewed in the display using a general format which is followed from one menu to the next. Each menu is a list of choices or selections. There are five lines in the display. The top line in each menu is a title or pertinent instruction. The top line does not change unless a new menu is selected. The second line is used in two ways.
inner menu to the MAIN MENU by repeatedly pushing the EXIT button. Pushing OFF at any time will turn the spectrophotometer off. CALIBRATION CALIBRATE WAVELENGTH The Calibrate Wavelength (CALIBRATE WL) mode is used to establish or re-establish the accuracy of the wavelength selection process. Normally, the Calibrate Wavelength procedure should be run after the SMART Spectro is turned ON and allowed to warm up for 15 minutes or if operating conditions (temperature, humidity, etc.) change significantly.
will then move the grating a precise predetermined amount from this position. This precise movement will cause the grating to be positioned at 546 nm every time. Once calibrated the wavelength displayed during testing is accurate to ±2 nm. When the wavelength calibration is complete the display will go back to the Main Menu. PROGRAMMED TESTS INTRODUCTION The PROGRAMMED TESTS mode is used to run all LaMotte pre-programmed tests and USER TESTS.
SEQUENCES OF TESTS SEQUENCE 1, SEQUENCE 2, and SEQUENCE 3 are alterable sequences. They may be edited using the EDIT function mode. Any of the LaMotte preprogrammed tests or User Tests may be placed in these sequences in whatever testing order that is preferred. Some examples of typical sequences are given below.
GENERAL TESTING PROCEDURES The following are some step by step examples of how to run tests from the PROGRAMMED TESTS menu. These test procedures are designed to be used with LaMotte SMART Spectro reagent systems. TESTING WITH THE LaMOTTE PROGRAMMED TESTS 1. Press ON. The LaMotte logo screen will appear for about 2 seconds and the MAIN MENU will appear. MAIN MENU 12:00:01 * CALIBRATE WL PROGRAMMED TESTS %T/ABS PC LINK MAIN MENU 12:00:01 2. Press or to scroll to PROGRAMMED TESTS.
5. Press *ENTER to select ALL TESTS. ALL TESTS * 1 Alkalinity-UDV 2 Aluminum 3 Ammonia-N L F 4 Ammonia-N L S 6. Press or desired test. ALL TESTS to scroll to the 1 Alkalinity-UDV * 2 Aluminum 3 Ammonia-N L F 4 Ammonia-N L S 7. Press *ENTER to select the test. The SMART Spectro is ready to scan. The proper wavelength has been selected. 2 Aluminum * SCAN BLANK SCAN SAMPLE END 8. Insert the blank. Press *ENTER to scan the blank. The blank has been stored. 9. Insert the reacted sample.
10.Press *ENTER to print the result when connected to a printer or computer. To repeat the test, press EXIT to escape the test screen, then press *ENTER to scan the sample again. The last blank scanned is used to zero the spectrophotometer for repeat scans. A different blank can be used by pressing the button to scroll back to SCAN BLANK and then scanning another blank. Press EXIT to escape back to the PROGRAMMED TESTS menu if no more samples are to be scanned for this test factor.
SETUP & EDIT SEQUENCES & USER TESTS The EDIT menu allows any of the three alterable test sequences (SEQUENCE 1, SEQUENCE 2, and SEQUENCE 3) and any of the 25 User Tests in the ALL TESTS fixed sequence to be edited. This feature allows a sequence or test which is used frequently to be set-up for easy access. The order of the sequence can be arranged to suit the needs of the user. Any combination, and any order of tests from ALL TESTS (including User Tests), may be placed into these sequences.
ADDING OR DELETING TESTS There are two ways to alter a sequence - INSERT and DELETE. INSERT is used to add a new test to a sequence and to place the new test before an existing test in a sequence. DELETE is used to remove an existing test from a sequence. Below is a step by step example of how to ADD a test to SEQUENCE 3 starting from the EDIT menu. EDIT 1. Press to scroll to the sequence to be edited. EDIT SEQUENCE 1 EDIT SEQUENCE 2 * 2. Press *ENTER to select the sequence to be edited. 3.
5. Press or desired test. INSERT to scroll to the 1 Alkalinity-UDV * 2 Aluminum 3 Ammonia-N L F 4 Ammonia-N LF 6. Press *ENTER to select the test and insert it before END OF LIST. The meter will return to the EDIT SEQUENCE 3 menu. 7. Press *ENTER to select the first test in the sequence and allow a second test to be inserted above it on the list. EDIT SEQUENCE 3 * 2 Aluminum END OF LIST EDIT SEQUENCE 3 * INSERT DELETE 2 Aluminum 8. Press *ENTER to insert a test above the first test.
10.Press *ENTER to add the second test to the list above the first test in the sequence. SEQUENCE 3 has now been modified and will remain until it is deleted again. To run a test in SEQUENCE 3 go to the PROGRAMMED TESTS menu. Press EXIT to exit the EDIT SEQUENCE 3 menu and return to the EDIT menu. Press EXIT to exit the EDIT menu. The meter will save any changes and go to the PROGRAMED TESTS menu.
4. Press *ENTER to select the test to be deleted. EDIT SEQUENCE 3 * INSERT DELETE 2 Aluminum 5. Press or DELETE. EDIT SEQUENCE 3 to scroll to INSERT * 6. Press *ENTER to select DELETE. Sequence 3 has now been modified and will remain until it is edited again. To run a test in SEQUENCE 3 go to the PROGRAMMED TESTS menu. Press ENTER to exit the EDIT SEQUENCE 3 menu to the EDIT menu. Press EXIT to exit the EDIT menu. The meter will save any changes and go the PROGRAMMED TESTS menu.
EDIT USER TESTS If a test other than the LaMotte programmed tests is performed regularly, a calibration for it may be entered in one of the 25 User Tests. These tests are originally named “User Test 1 - 25”. It will be possible to rename the test, select a wavelength, enter a new calibration and select the number of decimal places used to display the results. A User Test may be added for a reagent system for which no precalibrated test exists. A calibration of a LaMotte reagent system may also be entered.
3. Press TESTS. EDIT to scroll to EDIT USER EDIT SEQUENCE 1 EDIT SEQUENCE 2 EDIT SEQUENCE 3 * 4. Press *ENTER to select EDIT USER TESTS. EDIT USER TESTS EDIT * 101 User Test 1 102 User Test 2 103 User Test 3 104 User Test 4 EDIT 5. Press or to scroll to the user test to be edited. 101 User Test 1 * 102 User Test 2 103 User Test 3 104 User Test 4 6. Press *ENTER to select the user test to be edited.
NAMING THE TEST A NAME can be up to 14 characters long. The menu choices for each character are 26 letters A to Z, ten numerals 0 to 9, a space, a dash, a decimal point, and a ! as a terminator. Selecting the terminator indicates the end of the name and stores the name. The terminator, !, is the first menu choice since it is the one character that will always have to be selected. It is before the letter A. DO NOT CHOOSE THE ! UNTIL THE NAMING OF THE TEST HAS BEEN COMPLETED.
4. Select the characters in the name one at a time, from left to right. In this example the new test name will be H2O. Press to scroll right to the first character. Selection of characters is controlled by moving the curser over the top of the letter to be chosen. Press *ENTER with the curser over the character to select it. The character will appear now in the line next to the test number. Continue in this fashion until entire name is entered. Press EXIT to delete/erase any incorrect entry and start over.
SELECTING THE WAVELENGTH 1. Scroll to SELECT WL. 102 H2O NAME THE TEST * SELECT WL NEW CALIBRATION FORMAT RESULT 2. Press *ENTER to select SELECT WL. SELECT WL 546 NM SET PRESS ENTER 3. Press or wavelength. SELECT WL to scroll to desired 560 NM SET PRESS ENTER 4. Press *ENTER to save the wavelength. The display will return to the USER TEST menu. 102 H2O * NAME THE TEST SELECT WL NEW CALIBRATION FORMAT RESULT SMART Spectro Operator’s Manual 2.
ENTERING A NEW CALIBRATION 102 H2O 1. Press to scroll to NEW CALIBRATION. NAME THE TEST SELECT WL * 2. Press *ENTER to select NEW CALIBRATION. NEW CALIBRATION FORMAT RESULT INPUT DATA NUMBERS DATA NUMBERS = 5 SET PRESS ENTER INPUT DATA NUMBERS 3. Press or to select the number of data points that will be used to create the calibration curve. The maximum number of data points is 8. DATA NUMBERS = 6 SET PRESS ENTER 4. Press *ENTER to save the selection. NEW CALIBRATION * ENTER STD.1 ENTER STD.
6. Place a blank in the sample chamber. Press *ENTER to SCAN BLANK. (STD. 1 is always a blank.) The display will indicate that the blank is being scanned. The display will indicate BLANKED. A cursor will appear on the character selection line. 7. Place the reacted standard of the lowest concentration in the chamber. Press or to enter the concentration of the standard by selecting the characters with the cursor. 8. After the concentration has been entered, scroll to !. Press *ENTER.
NEW CALIBRATION 11.Press to scroll to CALCULATE. ENTER STD. 6 ENTER STD. 7 ENTER STD. 8 * 12.Press *ENTER to select CALCULATE. CALCULATE SELECT DEGREES 1 DEGREE THRU.0 1 DEGREE 2 DEGREE * 13.Press *ENTER to select the desired curve fit. 3 DEGREE SELECT DEGREES 1 DEGREE THRU.0 * 1 DEGREE 2 DEGREE 3 DEGREE Note: 1 DEGREE THRU.0 calculates the best straight line fit through the data points and intersects with the origin at 0 ppm, 0 absorbance. This is a classical Beers Law calibration.
15.Press EXIT to return to the SELECT DEGREES menu. Press EXIT again to return to INPUT DATA NUMBERS. Press EXIT again to return to the USER TEST menu. 102 H2O * NAME THE TEST SELECT WL NEW CALIBRATION FORMAT RESULT SELECTING THE NUMERICAL FORMAT OF THE RESULT To input tests with very different ranges, the number of decimal places displayed for a result can be selected. A test which ranges from 20 to 1000 ppm should not be displayed with three decimal places. A test with a range from 0.010 to 0.
4. Press *ENTER to select the number format. 102 H2O * NAME THE TEST SELECT WL NEW CALIBRATION FORMAT RESULT 5. Press EXIT to return to EDIT USER TEST menu. Press EXIT again to escape to EDIT menu and again to return to the PROGRAMMED TEST menu. Note: Test 102 was USER TEST 1 and now is H2O. It is still a USER TEST because its calibration can be changed but it has a different name. 102 H2O * NAME THE TEST SELECT WL NEW CALIBRATION FORMAT RESULT MEASURING IN THE %T/ABS MODE 1. Press ON.
4. Press *ENTER to select SELECT WL SELECT WL WL=560 NM * 5. Press or wavelength. to scroll to desired * SELECT WL WL=600 NM 6. Press *ENTER to select the wavelength. The meter is ready to scan. %T/ABS 600 NM SELECT WL * SCAN BLANK SCAN SAMPLE END OF LIST 7. Insert the blank into the chamber. Press *ENTER to scan the blank. Note: For most %T/ABS tests, a clear colorless water blank should be used. 8. Insert a reacted sample into the chamber. Press *ENTER to scan the sample.
PC LINK The SMART Spectro may be interfaced with any Windows-based program by using the LaMotte SMARTLink2 Program and Interface Cable (Code 1912-3) and compact disk (Code 1912-CD). The program stores customer information and test data in a database. It can be used to download data stored in the SMART Spectro data logger for each test site.
EDIT CLOCK The clock information is used to time stamp the data points in the data logger. 1. Press ON. The LaMotte logo screen will appear for about 2 seconds and the MAIN MENU will appear. MAIN MENU 12:00:01 * CALIBRATE WL PROGRAMMED TESTS %T/ABS PC LINK 2. Press or EDIT CLOCK. MAIN MENU 12:00:01 to scroll to PROGRAMMED TESTS %T/ABS PC LINK * 3. Press *ENTER to select EDIT CLOCK. The display will show seconds-minutes-hours-monthday-year. Note: Hours are in a 24 hour format. 4.
6. Follow the procedure to set the minutes, hour, month, day, and year. Press EXIT to return to the MAIN MENU. MAIN MENU 14:09:43 * CALIBRATE WL PROGRAMMED TESTS %T/ABS PC LINK ENERGY MODE 1. Press ON. The LaMotte logo screen will appear for about 2 seconds and the MAIN MENU will appear. MAIN MENU 12:00:01 * CALIBRATE WL PROGRAMMED TESTS %T/ABS PC LINK MAIN MENU 12:00:01 2. Press or to scroll to ENERGY MODE. %T/ABS PC LINK EDIT CLOCK * 3. Press *ENTER to select ENERGY MODE.
5. Press EXIT to return to the MAIN MENU. MAIN MENU 12:00:01 * CALIBRATE WL PROGRAMMED TESTS %T/ABS PC LINK STORE METHOD 1. Press ON. The LaMotte logo screen will appear for about 2 seconds and the MAIN MENU will appear. MAIN MENU 12:00:01 * CALIBRATE WL PROGRAMMED TESTS %T/ABS PC LINK MAIN MENU 12:00:01 2. Press or to scroll to STORE METHOD. PC LINK EDIT CLOCK ENERGY MODE * 3. Press *ENTER to select STORE METHOD. STORE METHOD STORE METHOD * AUTOMATIC MANUAL END OF LIST 4.
5. Press *ENTER to select the store method and return to the MAIN MENU. MAIN MENU 12:00:01 * CALIBRATE WL PROGRAMMED TESTS %T/ABS PC LINK TEST MODE Select TEST MODE from the Main Menu. Test Mode allows the user to change the way that blanking and reading of results is carried out during the %T/ABS testing. It does not affect any of the Pre-programmed tests. REGULAR is the default test mode and should be used whenever the blank is clear or has less absorbance than the samples.
4. Press or to select the desired test mode. TEST MODE * REGULAR REVERSE END OF LIST 5. Press *ENTER to select the test mode and return to the MAIN MENU. MAIN MENU 12:00:01 * CALIBRATE WL PROGRAMMED TESTS %T/ABS PC LINK BATTERY OPERATION The SMART Spectro can be run using battery power. The battery pack consists of a rechargeable Ni-metal hydride battery pack. The battery pack is not included in the standard meter package and must be purchased as an accessory (Code 2000-BP).
MAINTENANCE CLEANING Clean with a damp, lint-free cloth. DO NOT ALLOW WATER TO ENTER THE SPECTROPHOTOMETER CHAMBER OR ANY OTHER PARTS OF THE METER. LIGHT BULB The quartz halogen bulb in the SMART Spectro has an approximate life of 1000 hours. If a test is performed and there is no response and the unit is receiving adequate power, the light bulb may need to be re-secured or replaced. With the meter power ON check the rear lamp access panel to see if any light acan be observed through the vents.
touch the electronic components. You do not need to touch the electronic components to replace the RAM battery. If you will be touching the electronic components for any other reason, you must wear a grounding strap. If such a strap is not available then take every precaution possible to ground yourself before and during any contact with the electronic components by holding onto a grounded metal pipe or other grounded piece of metal.
TROUBLESHOOTING GUIDE ERROR MESSAGES OUT OF RANGE If the message OUT OF RANGE is displayed when scanning a sample, the sample may be over range or under range. If the sample is over range the sample should be diluted and tested again (see Sample Dilution Techniques & Volumetric Measurements, page16). BATTERY (optional) If the symbol BAT appears in the upper left corner of the display when using the battery pack, the battery needs to be charged.
HELPFUL HINTS POWER The power supply has an internal switch that handles both 110V and 220V input. STRAY LIGHT The SMART Spectro should have no problems with stray light. Make sure that the sample compartment lid is always fully closed. ACCIDENTAL LOSS OF POWER If for any reason the meter experiences a loss of power during wavelength calibration, the next time the meter is powered up a wavelength calibration will automatically be performed. SMART Spectro Operator’s Manual 2.
TEST PROCEDURES v3.0 and higher • Printed 2.
SPECTROPHOTOMETER REAGENT SYSTEMS LIST Call LaMotte Technical Services at 1-800-344-3100 (410-778-3100 outside the USA) or email at tech@lamotte.com for a current list of available calibrations and downloading instructions. Test Factor (Test #) Range (ppm) MDL Test Method (# of Reagents) # of Tests Alkalinity-UDV (2) 0–200 15 Unit Dose Vials (1) 100 Aluminum (1) 0.00–0.30 0.01 Eriochrome Cyanine R (4) 50 Ammonia NitrogenLow Range, Fresh Water (3) 0.00–1.00 0.
Color (28) 0–1,000 15 Platinum Cobalt (0) – Copper-BCA-Low Range (29) 0.00–3.50 0.05 Bicinchoninic Acid (1) 50 Copper-Cuprizone (31) 0.00–2.00 0.01 Cuprizone (2) 50 Copper-DDC (32) 0.00–6.00 0.05 Diethyldithiocarbamate (1) 50 Copper-UDV (33) 0.00–4.00 0.20 Bicinchoninic Acid (1) 100 Cyanide (35) 0.00-0.50 0.50 Pyridine-Barbituric Acid 100 Cyanuric Acid (36) 0–200 16 Melamine (1) 40 Cyanuric Acid-UDV (37) 0–150 5 Melamine (1) 100 DEHA (037) See Oxygen Scavengers 0.
Nitrate NitrogenLow Range (64) 0.00–3.00 0.05 Cadmium Reduction (2) 20 Nitrite-TesTab (69) 0.00-1.25 0.025 Zinc Reduction (1) 50 Nitrite NitrogenLow Range (67) 0.00–0.80 0.02 Diazotization (2) 20 Nitrogen, Total (62) 0-25 mg/L 2 mg/L Chromotropic Acid/ Digestion (6) 25 Oxygen Scanvengers various various DEHA (3) 50 Ozone-Low Range (071) 0.00–0.40 0.02 Indigo Trisulfonate (3) 100 Ozone-High Range (072) 0.00–1.50 0.05 Indigo Trisulfonate (3) 20 pH-Chlorophenol Red (073) 5.
ALKALINITY, UDV UNIT DOSE VIAL METHOD • CODE 4318-J-01 QUANTITY CONTENTS CODE 1 Alkalinity Unit Dose Vials, 20 pouches 4318-J Equipment needed but not supplied: STANDARD ACCESSORY PACKAGE • CODE 1961 1 Package of 3 Vials (empty) 0156 1 Syringe, 6 mL, plastic 1184 1 Foil Storage Bag 9467 Or: ADVANCED ACCESSORY PACKAGE • CODE 1962 Pipettor 30528 1 Pipet Tip (0-5 mL) 30695 1 Cuvette Rack 31695 1 Package of 3 Vials (empty) 0156 1 Foil Storage Bag 9467 *WARNING: Reagents marked with
Drinking and surface water and swimming pool water RANGE: 0–200 ppm as CaCO3 MDL: 15 ppm METHOD: The sample is added to a buffered indicator reagent. The color that develops, ranging from yellow to blue, will indicate the amount of alkalinity in the sample. SAMPLE HANDLING & PRESERVATION: Samples should be analyzed as soon as possible after collection. Sample may be refrigerated for 24 hours. INTERFERENCES: Quats and poly quats at high concentrations will interfere.
PROCEDURE 1. Use 10 mm square cell adapter 2. Press and hold ON button until spectrophotometer turns on. 3. Scroll to and select PROGRAMMED TESTS. 4. Scroll to and select ALL TESTS (or another sequence containing 2 Alk-UDV) from TESTING MENU. 5. Scroll to and select 2 Alk-UDV from menu. 6. Rinse a clean vial (0156) with sample water. 7. Use the syringe (1184) to add 3 mL of sample to the vial. 8. Insert the vial into chamber, close lid and select SCAN BLANK. 9. Remove vial from Spectro.
Test Procedures ALKALINITY, UDV SMART Spectro Test Procedures 2.
ALUMINUM ERIOCHROME CYANINE R METHOD • CODE 364I-SC QUANTITY CONTENTS CODE 5g *Aluminum Inhibitor Reagent *7865-C 2 x 120 mL *Aluminum Buffer Reagent *7866-J 120 mL Aluminum Indicator Reagent 7867-J 15 mL Aluminum Complexing Reagent 7868-E 1 Spoon, 0.1 g, plastic 0699 1 Spoon, 0.05 g, plastic 0696 2 Pipets, 1.0 mL, plastic 0354 1 Test Tube, glass, 5 mL w/cap 0230 *WARNING: Reagents marked with an * are considered to be potential health hazards.
PROCEDURE Use universal sample holder. 1. Press and hold ON button until spectrophotometer turns on. 2. Scroll to and select PROGRAMMED TESTS. 3. Scroll to and select ALL TESTS (or another sequence containing 1 Aluminum). 4. Scroll to and select 1 Aluminum from menu. 5. Rinse a clean Spectro tube (0290) with sample water. Fill to the 10 mL line with sample. 6. Insert tube into Spectro chamber and select SCAN BLANK. 7. Rinse a clean test tube (0230) with sample water.
AMMONIA-NITROGEN, LOW RANGE SALICYLATE METHOD • CODE 3659-01-SC QUANTITY CONTENTS CODE 60 mL *Salicylate Ammonia #1 *3978-H 10 g *Salicylate #2 *7457-D 2x5g *Salicylate #3 Reagent Powder *7458-C 1 Spoon, 0.1 g, plastic 0699 1 Spoon, 0.15 g, plastic 0727 1 Pipet, 1.0 mL, plastic 0354 *WARNING: Reagents marked with an * are considered to be potential health hazards. To view or print a Material Safety Data Sheet (MSDS) for these reagents go to www.lamotte.com.
Low concentrations of ammonia in fresh, brackish and salt water; fresh and salt water aquariums. RANGE: 0.00–1.00 ppm Ammonia-Nitrogen MDL: 0.02 ppm Fresh Waer 0.10 ppm Salt Water METHOD: Salicylate and ammonia react at high pH in the presence of a chlorine donor and an iron catalyst to form a blue indophenol dye, the concentration of which is proportional to the ammonia concentration in the sample.
PROCEDURE–FRESH WATER Use universal sample holder. 1. Press and hold ON button until spectrophotometer turns on. 2. Scroll to and select PROGRAMMED TESTS from menu. 3. Scroll to and select ALL TESTS (or another sequence containing 3 Ammonia-N LF) from TESTING MENU. 4. Scroll to and select 3 Ammonia-N LF from menu. 5. Rinse a clean tube (0290) with sample water. Fill to the 10 mL line with sample. 6. Insert tube into chamber, close lid and select SCAN BLANK. (See Note.) 7.
To determine the percentage of Ammonia-Nitrogen that is unionized and ionized, consult the Appendix. PROCEDURE–SALT WATER Test Procedures Use universal sample holder. 1. Press and hold ON button until spectrophotometer turns on. 2. Scroll to and select PROGRAMMED TESTS from menu. 3. Scroll to and select ALL TESTS (or another sequence containing 4 Ammonia-N LS) from TESTING MENU. 4. Scroll to and select 4 Ammonia-N LS from menu. 5. Rinse a clean tube (0290) with sample water.
CALCULATIONS: To express results as Unionized Ammonia (NH3): ppm Unionized Ammonia (NH3) = ppm Ammonia-Nitrogen (NH3–N) x 1.2 To express results as Ionized Ammonia (NH4): ppm Ionized Ammonia (NH4+) = ppm Ammonia-Nitrogen (NH3–N) x 1.3 NOTES: For the best possible results, a reagent blank should be determined to account for any contribution to the test result by the reagent system. To determine the reagent blank, follow the above test procedure to scan a distilled or deionized water blank.
Test Procedures AMMONIA-NITROGEN, Low Range SMART Spectro Test Procedures 2.
AMMONIA-NITROGEN, HIGH RANGE NESSLERIZATION METHOD • CODE 3642-SC QUANTITY CONTENTS CODE 30 mL Ammonia Nitrogen Reagent #1 V-4797-G *Ammonia Nitrogen Reagent #2 *V-4798-G 2 x 30 mL 1 Pipet, 1 mL, plastic 0354 *WARNING: Reagents marked with an * are considered to be potential health hazards. To view or print a Material Safety Data Sheet (MSDS) for these reagents go to www.lamotte.com. To obtain a printed copy, contact LaMotte by e-mail, phone or fax.
PROCEDURE Use universal sample holder. 1. Press and hold ON button until spectrophotometer turns on. 2. Scroll to and select PROGRAMMED TESTS. 3. Scroll to and select ALL TESTS (or another sequence containing 5 Ammonia-N H) from TESTING MENU. 4. Scroll to and select 5 Ammonia-N H from menu. 5. Rinse a clean tube (0290) with sample water. Fill to the 10 mL line with sample. 6. Insert tube into chamber, close lid and select SCAN BLANK. (See Note) 7. Remove tube from Spectro.
BIGUANIDE COLORIMETRIC METHOD • CODE 4044 QUANTITY CONTENTS CODE 2 X 60 mL Biguanide Indicator 3994-H 1 Pipet, plastic, 1.0 mL 0354 Biguanide is a non-chlorine, non-bromine chemical sanitizer. It is more stable than chlorine or bromine and has little chemical odor. Biquanide is an effective bacteriacide but, unlike chlorine and bromine, it does not destroy organic contaminants. Therefore, hydrogen peroxide is added to biguanide pools on a regular basis to eliminate organic contaminants.
PROCEDURE Use universal sample holder 1. Press and hold ON button until spectrophotometer turns on. 2. Scroll to and select PROGRAMMED TESTS. 3. Select ALL TESTS (or another sequence containing 7 Biguanide from TESTING MENU. 4. Scroll to and select 7 Biguanide from menu. 5. Rinse a tube (0290) with sample water. Fill to 10 mL with sample. 6. Insert the tube into chamber, close lid and select SCAN BLANK. 7. Remove the tube from colorimeter. 8. Use the 1.0 mL pipet (0354) to add 2.
BORON AZOMETHINE-H METHOD • CODE 4868 QUANTITY CONTENTS CODE 120 mL *Boron Buffer *4869-J 10 g *Boron Indicator Powder *4870-D 1 Pipet, plastic, 1.0 mL 0354 1 Spoon, 0.15 g 0727 1 Dark Storage Chamber, brown 0108 *WARNING: Reagents marked with an * are considered to be potential health hazards. To view or print a Material Safety Data Sheet (MSDS) for these reagents go to www.lamotte.com. To obtain a printed copy, contact LaMotte by e-mail, phone or fax.
PROCEDURE Test Procedures Use universal sample holder 1. This test requires a Reagent Blank. Rinse a tube (0290) with clear, colorless, boron free water. Fill to 10 mL line with clear, colorless, boron free water. 2. Use the 1.0 mL pipet (0354) to add 2 mL of *Boron Buffer (4869). Cap and mix. 3. Use the 0.15 g spoon (0727) to add one level measure of *Boron Indicator Powder (4870). Press full spoon against side of jar to compress powder. Scrape off excess powder on inside neck of bottle.
BROMINE, UDV DPD UNIT DOSE VIAL METHOD • CODE 4311-J QUANTITY 1 CONTENTS *Free Chlorine Unit Dose Vials, 20 pouches CODE *4311-J Equipment needed but not supplied: STANDARD ACCESSORY PACKAGE • CODE 1961 1 Package of 3 Vials (empty) 0156 1 Syringe, 3 mL, plastic 1184 1 Foil Storage Bag 9467 Or: ADVANCED ACCESSORY PACKAGE • CODE 1962 Pipettor, 3 mL 30528 1 Pipet Tip (0-5 mL) 30695 1 Cuvette Rack 31695 1 Package of 3 Vials (empty) 0156 1 Foil Storage Bag 9467 *WARNING: Reagents marked
APPLICATION: Drinking, surface, and saline waters; domestic and industrial waters and wastes. RANGE: 0.0 – 22.0 ppm Bromine MDL: 0.3 ppm METHOD: In buffered sample bromine reacts with diethyl-pphenylene diamine (DPD) to produce a pink-red color in proportion to the concentration of bromine present. SAMPLE HANDLING & PRESERVATION: Bromine in aqueous solutions is not stable, and the bromine content of samples or solutions, particularly weak solutions, will rapidly decrease.
PROCEDURE Use 10 mm square cell adapter 1. Press and hold ON button until spectrophotometer turns on. 2. Scroll to and select PROGRAMMED TESTS. 3. Scroll to and select ALL TESTS (or another sequence containing 11 Bromine-UDV) from TESTING MENU. 4. Scroll to and select 11 Bromine-UDV from menu. 5. Rinse a clean vial (0156) with sample water. 6. Use the syringe (1184) to add 3mL of sample to the vial. 7. Insert the vial into chamber, close the lid and select SCAN BLANK. 8.
Test Procedures BROMINE, UDV SMART Spectro Test Procedures 2.
CADMIUM PAN METHOD • CODE 4017-01 QUANTITY CONTENTS CODE 60 mL Buffered Ammonia Reagent 4020-H 15 mL Sodium Citrate, 10% 6253-E 30 mL *PAN Indicator *4021-G 30 mL Stabilizing Reagent 4022-G 1 Pipet, 1.0 mL, plastic 0354 2 Pipet, 0.5 mL, plastic 0369 *WARNING: Reagents marked with an * are considered to be potential health hazards. To view or print a Material Safety Data Sheet (MSDS) for these reagents go to www.lamotte.com.
PROCEDURE 1. Use universal sample holder. 2. Press and hold ON button until spectrophotometer turns on. 3. Scroll to and select PROGRAMMED TESTS. 4. Scroll to and select ALL TESTS (or another sequence containing 12 Cadmium) from TESTING MENU. 5. Scroll to and select 12 Cadmium from menu. 6. Rinse a tube (0290) with sample water. Fill to the 10 mL line with sample. 7. Insert the tube into chamber, close lid and select SCAN BLANK. 8. Remove tube from Spectro. Use the 1.
CALCIUM & MAGNESIUM (TOTAL) HARDNESS, UDV UNIT DOSE VIAL METHOD • CODE 4309-J QUANTITY CONTENTS CODE 1 Calcium Hardness Unit Dose Vials, 20 pouches 4309-J Equipment needed but not supplied: STANDARD ACCESSORY PACKAGE • CODE 1961 1 Package of 3 Vials (empty) 0156 1 Syringe, 3 mL, plastic 1184 1 Foil Storage Bag 9467 Or: ADVANCED ACCESSORY PACKAGE • CODE 1962 Pipettor, 3 mL 30528 1 Pipet Tips (0-5 mL) 30695 1 Cuvette Rack 31695 1 Package of 3 Vials (empty) 0156 1 Foil Storage Bag 9
PROCEDURE Use 10 mm square cell adapter 1. Press and hold ON button until spectrophotometer turns on. 2. Scroll to and select PROGRAMMED TESTS. 3. Scroll to and select ALL TESTS (or another sequence containing 13 Ca&Mg Hard-UDV) from TESTING MENU. 4. Scroll to and select 13 Ca&Mg Hard-UDV from menu. 5. Rinse a clean vial (0156) with sample water. 6. Use the syringe (1184) to add 3mL of sample to the vial. 7. Insert the vial into chamber, close lid and select SCAN BLANK. 8.
CHLORIDE, TESTAB ARGENTOMETRIC TESTAB METHOD • CODE 3693-SC QUANTITY 50 1 CONTENTS *Chloride Spectrophotometric Grade Tablets Tablet Crusher CODE *3885A-H 0175 *WARNING: Reagents marked with an * are considered to be potential health hazards. To view or print a Material Safety Data Sheet (MSDS) for these reagents go to www.lamotte.com. To obtain a printed copy, contact LaMotte by e-mail, phone or fax. Chloride is one of the major anions found in water and sewage.
PROCEDURE Use universal sample holder 1. Press and hold ON button until spectrophotometer turns on. 2. Scroll to and select PROGRAMMED TESTS. 3. Scroll to and select ALL TESTS (or another sequence containing 21 Chloride-TT) from TESTING MENU. 4. Scroll to and select 21 Chloride-TT from menu. 5. Rinse a tube (0290) with sample water. Fill to 10 mL with sample. 6. Insert the tube into chamber, close lid and select SCAN BLANK. 7. Remove the tube from Spectro. 8.
CHLORINE, BROMINE, IODINE DPD METHOD • CODE 3643-SC QUANTITY CONTENTS CODE 100 * DPD #1 Instrument Grade Tablets *6903A-J 100 *DPD #3 Instrument Grade Tablets *6197A-J 15 mL Glycine Solution 6811-E 1 Tablet Crusher 0175 *WARNING: Reagents marked with an * are considered to be potential health hazards. To view or print a Material Safety Data Sheet (MSDS) for these reagents go to www.lamotte.com. To obtain a printed copy, contact LaMotte by e-mail, phone or fax.
Drinking, surface, and saline waters; swimming pool water; domestic and industrial wastes. RANGE: 0.00–4.00 ppm Chlorine MDL: 0.02 ppm METHOD: In the absence of iodide, free available chlorine reacts instantly with DPD to produce a red color. Subsequent addition of potassium iodide evokes a rapid color response from the combined forms of chlorine (chloramines).
PROCEDURE–FREE CHLORINE Use universal sample holder. 1. Press and hold ON button until spectrophotometer turns on. 2. Scroll to and select PROGRAMMED TESTS. 3. Scroll to and select ALL TESTS (or another sequence containing 15 Chlorine) from TESTING MENU. 4. Scroll to and select 15 Chlorine from menu. 5. Rinse a clean tube (0290) with sample water. Fill to the 10 mL line with sample. 6. Insert tube into chamber, close lid and select SCAN BLANK. 7.
BROMINE Like chlorine, bromine is an effective germicidal agent employed in drinking water treatment, pool and spa water sanitation, food service sanitation, and other public health applications. APPLICATION: Drinking, surface, and saline waters; swimming pool water; domestic and industrial waters and wastes. RANGE: 0.00–9.00 ppm Bromine MDL: 0.
PROCEDURE A: BROMINE (NO CHLORINE) Use universal sample holder. 1. Press and hold ON button until spectrophotometer turns on. 2. Scroll to and select PROGRAMMED TESTS. 3. Scroll to and select ALL TESTS (or another sequence containing 9 Bromine-LR) from TESTING MENU. 4. Scroll to and select 9 Bromine-LR from menu. 5. Rinse a clean tube (0290) with sample water. Fill to the 10 mL line with sample. 6. Insert tube into chamber, close lid and select SCAN BLANK. 7. Remove tube from Spectro.
8. Insert tube into chamber, close lid and select SCAN SAMPLE. 9. Press OFF button to exit to previous menu or make another menu selection. PROCEDURE C: FREE AVAILABLE, TOTAL AVAILABLE & COMBINED CHLORINE IN THE PRESENCE OF BROMINE 1. Perform the test for free and combined chlorine as previously described. 2. Perform the test for bromine in the presence of chlorine. 3. Calculations: Residual Bromine (ppm) = Reading BR Free Chlorine in the Presence of Bromine = Free Chlorine – 0.
IODINE Like chlorine and bromine, iodine is an effective germicidal agent employed in drinking water treatment, pool and spa water sanitation, food service sanitation, and other public health applications. APPLICATION: Drinking, surface, and saline waters; swimming pool water; domestic and industrial wastes. RANGE: 0.00–14.00 ppm Iodine MDL: 0.
PROCEDURE Use universal sample holder. Press and hold ON button until spectrophotometer turns on. 2. Scroll to and select PROGRAMMED TESTS. 3. Scroll to and select ALL TESTS (or another sequence containing 50 Iodine) from TESTING MENU. 4. Scroll to and select 50 Iodine from menu. 5. Rinse a clean tube (0290) with sample water. Fill tube to the 10 mL line with sample. 6. Insert tube into chamber, close lid and select SCAN BLANK. 7. Remove tube from Spectro.
CHLORINE-FREE, UDV DPD UNIT DOSE VIAL METHOD • CODE 4311-J QUANTITY 1 CONTENTS *Free Chlorine Unit Dose Vials, 20 pouches CODE *4311-J Equipment needed but not supplied: STANDARD ACCESSORY PACKAGE • CODE 1961 1 Package of 3 Vials (empty) 0156 1 Syringe, 3 mL, plastic 1184 1 Foil Storage Bag 9467 Or: ADVANCED ACCESSORY PACKAGE • CODE 1962 Pipettor 30528 1 Pipet Tips (0-5 mL) 30695 1 Cuvette Rack 31695 1 Package of 3 Vials (empty) 0156 1 Foil Storage Bag 9467 *WARNING: Reagents marke
Test Procedures (bleach), granular calcium hypochlorite or as organic chlorine compounds. Chlorine is not present in natural water supplies; if it is present it is the result of chlorination of a water supply or of chlorinated compounds being discharged as waste from industrial operations. The presence of chlorine in concentrations above 0.5 parts per million should be considered evidence of pollution from chlorine treated effluents or from a process in which high concentrations of chlorine are used.
PROCEDURE Use 10 mm square cell adapter 1. Press and hold ON button until spectrophotometer turns on. 2. Scroll to and select PROGRAMMED TESTS. 3. Scroll to and select ALL TESTS (or another sequence containing 16 Cl Free-UDV) from TESTING MENU. 4. Scroll to and select 16 Cl Free-UDV from menu. 5. Rinse a clean vial (0156) with sample water. 6. Use the syringe (1184) to add 3mL of sample to the vial. 7. Insert the vial into chamber, close the lid and select SCAN BLANK. 8.
Test Procedures CHLORINE-FREE, UDV SMART Spectro Test Procedures 2.
CHLORINE LIQUID DPD METHOD • CODE 4859 QUANTITY CONTENTS CODE 30 mL DPD 1A Free Chlorine Reagent P-6740-G 30 mL *DPD 1B Free Chlorine Reagent *P-6741-G 30 mL *DPD 3 Total Chlorine Reagent *P-6743-G *WARNING: Reagents marked with an * are considered to be potential health hazards. To view or print a Material Safety Data Sheet (MSDS) for these reagents go to www.lamotte.com. To obtain a printed copy, contact LaMotte by e-mail, phone or fax.
APPLICATION: Drinking, surface, and saline waters; swimming pool water; domestic and industrial wastes. RANGE: 0.00–4.00 ppm Chlorine MDL: 0.025 ppm METHOD: In the absence of iodide, free available chlorine reacts instantly with DPD to produce a red color. Subsequent addition of potassium iodide evokes a rapid color response from the combined forms of chlorine (chloramines).
PROCEDURE–FREE CHLORINE Use universal sample holder 1. Press and hold ON button until spectrophotometer turns on. 2. Scroll to and select PROGRAMMED TESTS. 3. Scroll to and select ALL TESTS (or another sequence containing 17 Cl DPD-Liq) from TESTING MENU. 4. Scroll to and select 17 Cl DPD-Liq from menu. 5. Rinse a tube (0290) with sample water. Fill to the 10 mL line with sample. 6. Insert the tube into chamber, close lid and select SCAN BLANK. 7. Remove the tube from spectrophotometer. 8.
Test Procedures CHLORINE, DPD Liquid SMART Spectro Test Procedures 2.
CHLORINE–TOTAL, UDV DPD UNIT DOSE VIAL METHOD • CODE 4312-J QUANTITY 1 CONTENTS *Total Chlorine Unit Dose Vials, 20 pouches CODE *4312-J Equipment needed but not supplied: STANDARD ACCESSORY PACKAGE • CODE 1961 1 Package of 3 Vials (empty) 0156 1 Syringe, 3 mL, plastic 1184 1 Foil Storage Bag 9467 Or: ADVANCED ACCESSORY PACKAGE • CODE 1962 Pipettor, 3 mL 30528 1 Pipet Tips (0-5 mL) 30695 1 Cuvette Rack 31695 1 Package of 3 Vials (empty) 0156 1 Foil Storage Bag 9467 *WARNING: Reagen
Test Procedures (bleach), granular calcium hypochlorite or as organic chlorine compounds. Chlorine is not present in natural water supplies; if it is present it is the result of chlorination of a water supply or of chlorinated compounds being discharged as waste from industrial operations. The presence of chlorine in concentrations above 0.5 parts per million should be considered evidence of pollution from chlorine treated effluents or from a process in which high concentrations of chlorine are used.
PROCEDURE Use 10 mm square cell adapter 1. Press and hold ON button until spectrophotometer turns on. 2. Scroll to and select PROGRAMMED TESTS. 3. Scroll to and select ALL TESTS (or another sequence containing 18 Cl Total-UDV) from TESTING MENU. 4. Scroll to and select 18 Cl Total-UDV from menu. 5. Rinse a clean vial (0156) with sample water 6. Use the syringe (1184) to add 3mL of sample to the vial. 7. Insert the vial into chamber, close the lid and select SCAN BLANK. 8.
Test Procedures CHLORINE-TOTAL, UDV SMART Spectro Test Procedures 2.
CHLORINE DIOXIDE DPD METHOD • CODE 3644-SC QUANTITY 100 CONTENTS *DPD #1 Instrument Grade Tablets CODE *6903A-J 15 mL Glycine Solution 6811-E 1 Tablet Crusher 0175 *WARNING: Reagents marked with an * are considered to be potential health hazards. To view or print a Material Safety Data Sheet (MSDS) for these reagents go to www.lamotte.com. To obtain a printed copy, contact LaMotte by e-mail, phone or fax. Chlorine dioxide is used as a substitute for and an adjunct to chlorine in water treatment.
PROCEDURE Use universal sample holder. 1. Press and hold ON button until spectrophotometer turns on. 2. Scroll to and select PROGRAMMED TESTS from menu. 3. Scroll to and select ALL TESTS (or another sequence containing 20 CHLOR DIOX) from TESTING MENU. 4. Scroll to and select 20 CHLOR DIOX from menu. 5. Rinse a clean tube (0290) with sample water. Fill to 10 mL line with sample. 6. Insert tube into chamber, close lid and select SCAN BLANK. 7. Remove tube from Spectro.
CHROMIUM, HEXAVALENT DIPHENYLCARBOHYDRAZIDE METHOD CODE 3645-SC QUANTITY 10 g CONTENTS *Chromium Reagent Powder CODE *V-6276-D 1 Spoon, 0.1 g, plastic 0699 50 Filter Paper 0465-H 1 Funnel, Plastic 0459 *WARNING: Reagents marked with an * are considered to be potential health hazards. To view or print a Material Safety Data Sheet (MSDS) for these reagents go to www.lamotte.com. To obtain a printed copy, contact LaMotte by e-mail, phone or fax.
PROCEDURE Test Procedures Use universal sample holder. 1. Press and hold ON button until spectrophotometer turns on. 2. Scroll to and select PROGRAMMED TESTS from menu. 3. Scroll to and select ALL TESTS (or another sequence containing 22 Chromium) from TESTING MENU. 4. Scroll to and select 22 Chromium from menu. 5. Rinse a clean tube (0290) with sample water. Fill to the 10 mL line with sample. 6. Insert tube into chamber, close lid and select SCAN BLANK. 7. Remove tube from Spectro.
CHROMIUM-HEXAVALENT, TRIVALENT & TOTAL DIPHENYLCARBOHYDRAZIDE METHOD CODE 3698-SC QUANTITY CONTENTS CODE 60 mL *Sulfuric Acid, 5N *7681-H 10 g *Chromium Reagent Powder *V-6276-D 15 mL *Sodium Azide, 5% *7683-E Potassium Permanganate, 0.5% 7682-G 60 mL Deionized Water 5115PT-H 1 Pipet, plain, glass, w/cap 0341 1 Pipet, 1.0 mL, plastic 0354 1 Spoon, 0.
Drinking, surface, and saline waters; domestic and industrial wastes. RANGE: 0.00–1.00 ppm Chromium MDL: 0.01 ppm METHOD: The trivalent chromium is converted to hexavalent chromium by permanganate under acidic conditions. Hexavalent chromium reacts with 1,5 diphenylcarbohydrazide under acidic conditions to form a redpurple color in proportion to the amount of chromium present.
HEXAVALENT CHROMIUM PROCEDURE Use universal sample holder. 1. Press and hold ON button until spectrophotometer turns on. 2. Scroll to and select 22 Chromium from menu. 3. Scroll to and select ALL TESTS (or another sequence containing 22 Chromium) from TESTING MENU. 4. Scroll to and select 22 Chromium from menu. 5. Rinse a clean tube (0290) with sample water. Fill to 10 mL line with sample water. 6. Insert tube into chamber, close lid and select SCAN BLANK. 7. Remove tube from Spectro. Use 0.
until pink color disappears. 7. Remove flask from heat. Cool sample under running water. This is the digested sample. 8. Pour digested sample into clean graduated cylinder (0418). Dilute to the 50 mL line with Deionized Water (5115). 9. Press and hold ON button until spectrophotometer turns on. 10. Scroll to and select PROGRAMMED TESTS from menu. 11. Scroll to and select ALL TESTS (or another sequence containing 22 Chromium) from TESTING MENU. 12. Scroll to and select 22 Chromium from menu. 13.
CHROMIUM, TESTAB DIPHENYLCARBOHYDRAZIDE TESTAB METHOD CODE 3697-SC QUANTITY 50 CONTENTS *Chromium Spectrophotometric Grade Tablets 1 Tablet Crusher CODE *3889A-H 0175 *WARNING: Reagents marked with an * are considered to be potential health hazards. To view or print a Material Safety Data Sheet (MSDS) for these reagents go to www.lamotte.com. To obtain a printed copy, contact LaMotte by e-mail, phone or fax. Chromium is one of a class of heavy metals found in the bottom mud of polluted bodies of water.
PROCEDURE Use universal sample holder 1. Press and hold ON button until spectrophotometer turns on. 2. Scroll to and select PROGRAMMED TESTS. 3. Scroll to and select ALL TESTS (or another sequence containing 23 Chromium-TT) from TESTING MENU. 4. Scroll to and select 23 Chromium-TT from menu. 5. Rinse a tube (0290) with sample water. Fill to 10 mL with sample. 6. Insert the tube into chamber, close lid and select SCAN BLANK. 7. Remove the tube from Spectro. 8.
COBALT PAN METHOD • CODE 4851 QUANTITY CONTENTS CODE 60 mL *Cobalt Buffer *4852-H 60 mL *Cobalt Indicator Reagent *4853-H 30 mL *Stabilizer Solution *4854-G 2 Pipet, 1.0 mL, plastic 0354 1 Pipet, 0.5 mL, plastic 0353 *WARNING: Reagents marked with an * are considered to be potential health hazards. To view or print a Material Safety Data Sheet (MSDS) for these reagents go to www.lamotte.com. To obtain a printed copy, contact LaMotte by e-mail, phone or fax.
PROCEDURE Use universal sample holder. 1. Press and hold ON button until spectrophotometer turns on. 2. Scroll to and select PROGRAMMED TESTS. 3. Scroll to and select ALL TESTS (or another sequence containing 24 Cobalt) from TESTING MENU. 4. Scroll to and select 24 Cobalt from menu. 5. Rinse a tube (0290) with sample water. Fill to 10 mL with sample. 6. Insert the tube into chamber, close lid and select SCAN BLANK. 7. Remove the tube from Spectro. 8. Use the 1.
COD, LOW RANGE MERCURY FREE DIGESTION METHOD • Code 0072-SC MERCURY DIGESTION METHOD • Code 0075-SC QUANTITY CONTENTS CODE 25 *COD Low Level Mercury Free Tubes *0072-SC or 25 *COD Low Level Mercury Tubes *0075-SC *WARNING: Reagents marked with an * are considered to be potential health hazards. To view or print a Material Safety Data Sheet (MSDS) for these reagents go to www.lamotte.com. To obtain a printed copy, contact LaMotte by e-mail, phone or fax.
Domestic and industrial wastes. RANGE: 0–150 mg/L COD MDL: 5 mg/L METHOD: Dichromate in the presence of silver salts, at high temperature in a closed system, oxidizes most organic compounds to 95-100% of the theoretical amount. This process is called digestion. As dichromate oxidizes the organic compounds, the amount of yellow color is reduced. The remaining yellow color is measured colorimetrically at the 420 nm and is directly proportional to the COD of the sample.
PROCEDURE Use universal sample holder 1. Homogenize sample if necessary. 2. Preheat COD heater block to 150±2°C. 3. Remove cap from COD tube. Hold tube at a 45° angle. Use a volumetric pipet, to carefully add 2.0 mL sample water allowing the sample to run down the side of the tube. 4. Cap and mix thoroughly. 5. Rinse the outside of the tube with distilled water. Wipe dry with a paper towel. 6. Repeat steps 3 through 5 using 2.0 mL distilled water. This is the reagent blank. 7.
A reagent blank should be run with each set of samples and with each lot of reagents. The reacted blank will be stable if stored in the dark. To eliminate error caused by contamination, wash all glassware with 20% sulfuric acid. For greater accuracy, a minimum of three repetitions should be performed and the results averaged. Test Procedures Some samples may be digested completely in less than two hours.
COD, STANDARD RANGE MERCURY FREE DIGESTION METHOD • CODE 0073-SC MERCURY DIGESTION METHOD • CODE 0076-SC QUANTITY CONTENTS CODE 25 *COD Standard Level Mercury Free Tubes *0073-SC or 25 *COD Standard Level Mercury Tubes *0076-SC *WARNING: Reagents marked with an * are considered to be potential health hazards. To view or print a Material Safety Data Sheet (MSDS) for these reagents go to www.lamotte.com. To obtain a printed copy, contact LaMotte by e-mail, phone or fax.
Domestic and industrial wastes. RANGE: 0–1500 mg/L COD MDL: 20 mg/L METHOD: Dichromate in the presence of silver salts, at high temperature in a closed system, oxidizes most organic compounds to 95-100% of the theoretical amount. This process is called digestion. As dichromate oxidizes the organic compounds, a green complex is formed. The concentration of the green complex is measured at 620 nm and is directly proportional to the COD of the sample.
PROCEDURE Use universal sample holder 1. Homogenize sample if necessary. 2. Preheat COD heater block to 150±2°C. 3. Remove cap from COD tube. Hold tube at a 45° angle. Use a volumetric pipet, to carefully add 2.0 mL sample water allowing the sample to run down the side of the tube. 4. Cap and mix thoroughly. 5. Rinse the outside of the tube with distilled water. Wipe dry with a paper towel. 6. Repeat steps 2 through 5 using 2.0 mL distilled water. This is the reagent blank. 7.
A reagent blank should be run with each set of samples and with each lot of reagents. The reacted blank will be stable if stored in the dark. To eliminate error caused by contamination, wash all glassware with 20% sulfuric acid. For greater accuracy, a minimum of three repetitions should be performed and the results averaged. Test Procedures Some samples may be digested completely in less than two hours.
COD, HIGH RANGE MERCURY FREE DIGESTION METHOD • Code 0074-SC MERCURY DIGESTION METHOD • Code 0077-SC QUANTITY CONTENTS CODE 25 *COD High Level Mercury Free Tubes *0074-SC or 25 *COD High Level Mercury Tubes *0077-SC *WARNING: Reagents marked with an * are considered to be potential health hazards. To view or print a Material Safety Data Sheet (MSDS) for these reagents go to www.lamotte.com. To obtain a printed copy, contact LaMotte by e-mail, phone or fax.
APPLICATION: Domestic and industrial wastes. RANGE: 0–15,000 mg/L COD MDL: 500 mg/L METHOD: Dichromate in the presence of silver salts, at high temperature in a closed system, oxidizes most organic compounds to 95-100% of the theoretical amount. This process is called digestion. As dichromate oxidizes the organic compounds, a green complex is formed. The concentration of the green complex is measured at 605 nm and is directly proportional to the COD of the sample.
PROCEDURE Use universal sample holder 1. Homogenize sample if necessary. 2. Preheat COD heater block to 150±2°C. 3. Remove cap from COD tube. Hold vial at a 45° angle. Use a graduated pipet, to carefully add 0.2 mL sample water allowing the sample to run down the side of the tube. 4. Cap and mix thoroughly. 5. Rinse the outside of the tube with distilled water. Wipe dry with a paper towel. 6. Repeat steps 3 through 5 using 0.2 mL distilled water. This is the reagent blank. 7.
A reagent blank should be run with each set of samples and with each lot of reagents. The reacted blank will be stable if stored in the dark. To eliminate error caused by contamination, wash all glassware with 20% sulfuric acid. For greater accuracy, a minimum of three repetitions should be performed and the results averaged. Test Procedures Some samples may be digested completely in less than two hours.
COLOR PLATINUM COBALT METHOD NO REAGENTS REQUIRED Color in water may be attributed to humus, peat, plankton, vegetation, and natural metallic ions, such as iron and manganese, or industrial waste. Color is removed to make water suitable for domestic and industrial use. Color may have to be removed from industrial waste before it is discharged to a waterway. APPLICATION: Potable water and water with color due to natural materials.
PROCEDURE Use universal sample holder. 1. Press and hold ON burton until spectrophotometer turns on. 2. Scroll to and select PROGRAMMED TESTS. 3. Scroll to and select ALL TESTS (or another sequence containing 28 Color) from TESTING MENU. 4. Scroll to and select 28 Color from menu. 5. Rinse a tube (0290) with color-free water (distilled or deionized water). Fill to 10 mL line with color-free water. 6. Insert the tube into chamber, close lid and select SCAN BLANK. 7.
COPPER BCA, LOW RANGE BICINCHONINIC ACID METHOD • CODE 3640-SC QUANTITY 50 CONTENTS *Copper Tablets CODE *T-3808-H *WARNING: Reagents marked with an * are considered to be potential health hazards. To view or print a Material Safety Data Sheet (MSDS) for these reagents go to www.lamotte.com. To obtain a printed copy, contact LaMotte by e-mail, phone or fax. The copper content of drinking water generally falls below 0.03 parts per million, but copper levels as high as 1.
PROCEDURE Use universal sample holder. Press and hold ON button until spectrophotometer turns on. 2. Scroll to and select PROGRAMMED TEST from menu. 3. Scroll to and select ALL TESTS (or another sequence containing 29 Copper BCA-LR) from TESTING MENU. 4. Scroll to and select 29 Copper BCA-LR from menu. 5. Rinse a clean tube (0290) with sample water. Fill to the 10 mL line with sample. 6. Insert tube into chamber, close lid and select SCAN BLANK. 7.
COPPER CUPRIZONE METHOD • CODE 4023 QUANTITY CONTENTS CODE 15 mL Copper A P-6367-E 15 mL Copper B P-6368-E *WARNING: Reagents marked with an * are considered to be potential health hazards. To view or print a Material Safety Data Sheet (MSDS) for these reagents go to www.lamotte.com. To obtain a printed copy, contact LaMotte by e-mail, phone or fax. The copper content of drinking water generally falls below 0.03 parts per million, but copper levels as high as 1.
PROCEDURE Use universal sample holder. 1. Press and hold ON button until spectrophotometer turns on. 2. Scroll to and select PROGRAMMED TESTS. 3. Scroll to and select ALL TESTS (or another sequence containing 31 Cu-Cuprizone) from TESTING MENU. 4. Scroll to and select 31 Cu-Cuprizone from menu. 5. Rinse a tube (0290) with sample water. Fill to the 10 mL line with sample. 6. Insert the tube into chamber, close lid and select SCAN BLANK. 7.
COPPER, DDC DIETHYLDITHIOCARBAMATE METHOD • CODE 3646-SC QUANTITY 15 mL CONTENTS *Copper 1 CODE *6446-E *WARNING: Reagents marked with an * are considered to be potential health hazards. To view or print a Material Safety Data Sheet (MSDS) for these reagents go to www.lamotte.com. To obtain a printed copy, contact LaMotte by e-mail, phone or fax. The copper content of drinking water generally falls below 0.03 parts per million, but copper levels as high as 1.
PROCEDURE Use universal sample holder. 1. Press and hold ON button until spectrophotometer turns on. 2. Scroll to and select PROGRAMMED TESTS from menu. 3. Scroll to and select ALL TESTS (or another sequence containing 32 Copper DDC) from TESTING MENU. 4. Scroll to and select 32 Copper DDC from menu. 5. Rinse a clean tube (0290) with sample water. Fill to the 10 mL line with sample. 6. Insert tube into chamber, close lid and select SCAN BLANK. 7.
COPPER, UDV BICINCHONINIC ACID–UNIT DOSE VIAL METHOD CODE 4314-J QUANTITY CONTENTS CODE 1 Copper Unit Dose Vials, 20 pouches 4314-J Equipment needed but not suppled: STANDARD ACCESSORY PACKAGE • CODE 1961 1 Package of 3 Vials (empty) 0156 1 Syringe, 3 mL, plastic 1184 1 Foil Storage Bag 9467 Or: ADVANCED ACCESSORY PACKAGE • CODE 1962 Pipettor, 3 mL 30528 1 Pipet Tip (0-5 mL) 30695 1 Cuvette Rack 31695 1 Package of 3 Vials (empty) 0156 1 Foil Storage Bag 9467 *WARNING: Reagents m
Drinking, surface, and saline waters; domestic and industrial wastes. RANGE: 0.00–4.00 Copper MDL: 0.20 ppm METHOD: Cupric ions form a purple complex with bicinchoninic acid around pH 6–7, in proportion to the concentration of copper in the sample. SAMPLE HANDLING & PRESERVATION: Copper has a tendency to be adsorbed to the surface of the sample container. Samples should be analyzed as soon as possible after collection. If storage is necessary, 0.
PROCEDURE Use 10 mm square cell adapter. 1. Press and hold ON button until spectrophotometer turns on. 2. Scroll to and select PROGRAMMED TESTS. 3. Scroll to and select ALL TESTS (or another sequence containing 33 Copper-UDV) from TESTING MENU. 4. Scroll to and select 33 Copper-UDV from menu. 5. Rinse a clean vial (0156) with sample water. 6. Use the syringe (1184) to add 3 mL of sample to the vial. 7. Insert the vial into chamber, close lid and select SCAN BLANK. 8. Remove vial from Spectro.
Test Procedures COPPER, UDV SMART Spectro Test Procedures 2.
CYANIDE PYRIDINE-BARBITURIC ACID METHOD CODE 3660-SC QUANTITY CONTENTS CODE 60 mL Cyanide Buffer 2850PS-H 5g *Cyanide Cl Reagent *2794DS-C 5g *Cyanide Indicator Reagent *2793DS-C 15 mL *Hydrochloric Acid 1N *6130-E 15 mL *Sodium Hydroxide 1N *4004-E 2 Spoons, 0.1 g, plastic 0699 1 Pipet, plastic, 1.0 mL 0354 1 pH Short Range Test Paper, pH 9–14 2955 1 Stirring Rod, Plastic 0519 *WARNING: Reagents marked with an * are considered to be potential health hazards.
APPLICATION: Low level concentrations in drinking and surface waters; domestic and industrial waters. This method determines only those cyanides amenable to chlorination. RANGE: 0.00–0.500 ppm Cyanide MDL: 0.05 ppm METHOD: Cyanides react with a chlorine donor to form cyanogen chloride, which subsequently reacts with Pyridine and Barbituric Acid to form a red-blue compound in proportion to the amount of cyanide originally present.
PROCEDURE Use universal sample holder. 1. Press and hold ON button until spectrophotometer turns on. 2. Scroll to and select PROGRAMMED TESTS from menu. 3. Scroll to and select ALL TESTS (or another sequence containing 35 Cyanide) from TESTING MENU. 4. Scroll to and select 35 Cyanide from menu. 5. Rinse a clean tube (0290) with sample water. Fill to the 10 mL line with sample. 6.
Test Procedures CYANIDE SMART Spectro Test Procedures 2.
CYANURIC ACID MELAMINE METHOD–TURBIDITY • CODE 366I-01-SC QUANTITY 2 x 100 mL 1 CONTENTS *Cyanuric Acid Test Solution Syringe, 5 mL CODE *4856-J 0807 *WARNING: Reagents marked with an * are considered to be potential health hazards. To view or print a Material Safety Data Sheet (MSDS) for these reagents go to www.lamotte.com. To obtain a printed copy, contact LaMotte by e-mail, phone or fax. Cyanuric acid is added to swimming pool water as a stabilizing agent for free chlorine residuals.
PROCEDURE Test Procedures Use universal sample holder. 1. Press and hold ON button until spectrophotometer turns on. 2. Scroll to and select PROGRAMMED TESTS. 3. Scroll to and select ALL TESTS (or another sequence containing 36 Cyanuric) from TESTING MENU. 4. Scroll to and select 36 Cyanuric from menu. 5. Rinse a clean tube (0290) with sample water. Fill to the 10 mL line with sample. 6. Insert tube into chamber, close lid and select SCAN BLANK. 7. Remove tube from Spectro and pour out water.
CYANURIC ACID, UDV MELAMINE–TURBIDITY–UNIT DOSE VIAL METHOD CODE 4313-J QUANTITY CONTENTS CODE 1 Cyanuric Acid Unit Dose Vials, 20 pouches 4313-J Equipment but not suppled: STANDARD ACCESSORY PACKAGE • CODE 1961 1 Package of 3 Vials (empty) 0156 1 Syringe, 3 mL, plastic 1184 1 Foil Storage Bag 9467 Or: ADVANCED ACCESSORY PACKAGE • CODE 1962 Pipettor, 3 mL 30528 1 Pipet Tip (0-5 mL) 30695 1 Cuvette Rack 31695 1 Package of 3 Vials (empty) 0156 1 Foil Storage Bag 9467 *WARNING: Rea
Swimming pool water. RANGE: 0–150 Cyanuric Acid MDL: 5 ppm METHOD: A buffered solution of melamine forms a precipitate with cyanuric acid in proportion to the amount of cyanuric acid present. The amount of particles in suspension is measured turbidimetrically. SAMPLE HANDLING & PRESERVATION: Cyanuric acid samples should be analyzed as soon as possible after collection. Deterioration of the sample can be minimized by keeping samples in the dark or refrigerated until analysis can be performed.
PROCEDURE Use 10 mm square cell adapter. 1. Press and hold ON button until spectrophotometer turns on. 2. Scroll to and select PROGRAMMED TESTS. 3. Scroll to and select ALL TESTS (or another sequence containing 37 Cyanuric-UDV) from TESTING MENU. 4. Scroll to and select 37 Cyanuric-UDV from menu. 5. Rinse a clean vial (0156) with sample water. 6. Use the syringe (1184) to add 3 mL of sample to the vial. 7. Insert the vial into chamber, close lid and select SCAN BLANK. 8.
Test Procedures CYANURIC ACID, UDV SMART Spectro Test Procedures 2.
DISSOLVED OXYGEN WINKLER COLORIMETRIC METHOD • CODE 3688-SC QUANTITY CONTENTS CODE 30 mL *Manganese Sulfate Solution *4167-G 30 mL *Alkaline Potassium Iodide Azide *7166-G 30 mL *Sulfuric Acid 1:1 *6141WT-G 1 Sample Tube, screw cap 29180 1 Cap 28570 *WARNING: Reagents marked with an * are considered to be potential health hazards. To view or print a Material Safety Data Sheet (MSDS) for these reagents go to www.lamotte.com. To obtain a printed copy, contact LaMotte by e-mail, phone or fax.
COLLECTION & TREATMENT OF THE WATER SAMPLE Test Procedures Steps 1 through 4 below describe proper sampling technique in shallow water. For sample collection at depths beyond arm’s reach, special water sampling apparatus is required (e.g. the LaMotte Water Sampling Chamber, Code 1060; Model JT-1 Water Samplers, Code 1077; Water Sampling Outfit, Code 3103; or Water Sampling Bottle, Code 3-0026). 1. To avoid contamination, thoroughly rinse the screw cap Sample Tube (29180) with sample water. 2.
PROCEDURE Use universal sample holder. 1. Press and hold ON button until spectrophotometer turns on. 2. Scroll to and select PROGRAMMED TESTS. 3. Scroll to and select ALL TESTS (or another sequence containing 39 OXYGEN) from TESTING MENU. 4. Scroll to and select 39 OXYGEN from menu. 5. Rinse a clean tube (0290) with untreated sample water. Fill to the 10 mL line with sample. This tube is the BLANK. 6. Insert tube into chamber, close lid and select SCAN BLANK. 7.
Test Procedures DISSOLVED OXYGEN SMART Spectro Test Procedures 2.
FLUORIDE SPADNS METHOD • CODE 3647-02-SC QUANTITY CONTENTS CODE 4 x 30 mL *Acid-Zirconyl-SPADNS Reagent *3875-G 2 x 30 mL *Sodium Arsenite Solution *4128-G 1 Pipet, 0.5 mL, plastic 0353 1 Pipet, 1.0 mL, plastic 0354 *WARNING: Reagents marked with an * are considered to be potential health hazards. To view or print a Material Safety Data Sheet (MSDS) for these reagents go to www.lamotte.com. To obtain a printed copy, contact LaMotte by e-mail, phone or fax.
APPLICATION Drinking and surface waters; domestic and industrial waters. RANGE: 0.00–2.00 ppm Fluoride MDL: 0.05 ppm METHOD: Colorimetric test based upon the reaction between fluoride and zirconium dye lake. The fluoride reacts with the dye lake, dissociating a portion of it into a colorless complex ion and dye. As the fluoride concentration increases, the color produced becomes progressively lighter. SAMPLE HANDLING & PRESERVATION: Samples may be stored and refrigerated in plastic containers.
PROCEDURE Use universal sample holder. 1. Press and hold ON button until spectrophotometer turns on. 2. Scroll to and select PROGRAMMED TESTS. 3. Scroll to and select ALL TESTS (or another sequence containing 41 Fluoride) from TESTING MENU. 4. Scroll to and select 41 Fluoride from menu 5. This test requires a reagent blank. Rinse a clean tube (0290) with clear, colorless, fluoride free water. Fill to the 10 mL line with clear, colorless, fluoride free water. 6. Use the 0.
Test Procedures FLUORIDE SMART Spectro Test Procedures 2.
HYDRAZINE p-DIMETHYLAMINOBENZALDEHYDE METHOD CODE 3656-01-SC QUANTITY CONTENTS CODE 2x60 mL *Hydrazine Reagent A *4841-H 10 g *Hydrazine Reagent B Powder *4842-D 1 Pipet, 1.0 mL, plastic 0354 1 Spoon, 0.15 g, plastic 0727 *WARNING: Reagents marked with an * are considered to be potential health hazards. To view or print a Material Safety Data Sheet (MSDS) for these reagents go to www.lamotte.com. To obtain a printed copy, contact LaMotte by e-mail, phone or fax.
PROCEDURE Test Procedures Use universal sample holder. 1. Press and hold ON button until spectrophotometer turns on. 2. Scroll to and select PROGRAMMED TESTS. 3. Scroll to and select ALL TESTS (or another sequence containing 45 Hydrazine) from TESTING MENU. 4. Scroll to and select 45 Hydrazine from menu. 5. Rinse a clean tube (0290) with sample water. Fill to the 10 mL line with sample. 6. Insert tube into chamber, close lid and select SCAN BLANK. 7. Remove tube from Spectro.
HYDROGEN PEROXIDE, LOW RANGE DPD METHOD • CODE 3662-SC QUANTITY CONTENTS CODE 30 mL *Hydrogen Peroxide Reagent #1 *6452-G 100 *Hydrogen Peroxide LR Tablets *6454A-J 1 Tablet Crusher 0175 *WARNING: Reagents marked with an * are considered to be potential health hazards. To view or print a Material Safety Data Sheet (MSDS) for these reagents go to www.lamotte.com. To obtain a printed copy, contact LaMotte by e-mail, phone or fax.
PROCEDURE Test Procedures Use universal sample holder. 1. Press and hold ON button until spectrophotometer turns on. 2. Scroll to and select PROGRAMMED TESTS. 3. Scroll to and select ALL TESTS (or another sequence containing 46 H Peroxide-LR) from TESTING MENU. 4. Scroll to and select 46 H Peroxide-LR from menu. 5. Rinse a clean tube (0290) with sample water. Fill to the 10 mL line with sample. 6. Insert tube into chamber, close lid and select SCAN BLANK. (See Note) 7.
HYDROGEN PEROXIDE, HIGH RANGE DPD METHOD • CODE 4045-01 QUANTITY CONTENTS CODE 30 mL *Hydrogen Peroxide Reagent #1 *6452-G 100 *Hydrogen Peroxide LR Tablets *6454A-J 1 Tablet Crusher 0175 1 Pipet, glass 0342 *WARNING: Reagents marked with an * are considered to be potential health hazards. To view or print a Material Safety Data Sheet (MSDS) for these reagents go to www.lamotte.com. To obtain a printed copy, contact LaMotte by e-mail, phone or fax.
PROCEDURE Use universal sample holder 1. Press and hold ON button until spectrophotometer turns on. 2. Scroll to and select PROGRAMMED TESTS. 3. Select ALL TESTS (or another sequence containing 47 H Per-HR) from TESTING MENU. 4. Scroll to and select 47 H Per-HR from menu. 5. Use the pipet (0342) to add 5 drops of the sample water to a tube (0290). 6. Dilute to the 10 mL line with distilled or hydrogen peroxide-free water. 7. Insert the tube into chamber, close lid and select SCAN BLANK. 8.
HYDROGEN PEROXIDE, SHOCK DPD METHOD • CODE 4045 QUANTITY CONTENTS CODE 30 mL *Hydrogen Peroxide Reagent #1 *6452-G 100 *Hydrogen Peroxide LR Tablets *6454A-J 1 Tablet Crusher 0175 1 Pipet, glass 0342 *WARNING: Reagents marked with an * are considered to be potential health hazards. To view or print a Material Safety Data Sheet (MSDS) for these reagents go to www.lamotte.com. To obtain a printed copy, contact LaMotte by e-mail, phone or fax.
PROCEDURE Use universal sample holder 1. Press and hold ON button until spectrophotometer turns on. 2. Scroll to and select PROGRAMMED TESTS. 3. Select ALL TESTS (or another sequence containing 48 H Per Shock) from TESTING MENU. 4. Scroll to and select 48 H Per Shock from menu. 5. Use the pipet (0342) to add 5 drops of the sample water to a tube (0290). 6. Dilute to the 10 mL line with distilled or hydrogen peroxide-free water. 7. Insert the tube into chamber, close lid and select SCAN BLANK.
IRON BIPYRIDYL METHOD • CODE 3648-SC QUANTITY CONTENTS CODE 30 mL *Iron Reagent #1 *V-4450-G 5g *Iron Reagent #2 Powder *V-4451-C 1 Pipet, 0.5 mL, plastic 0353 1 Spoon, 0.1 g, plastic 0699 *WARNING: Reagents marked with an * are considered to be potential health hazards. To view or print a Material Safety Data Sheet (MSDS) for these reagents go to www.lamotte.com. To obtain a printed copy, contact LaMotte by e-mail, phone or fax.
PROCEDURE Test Procedures Use universal sample holder. 1. Press and hold ON button until spectrophotometer turns on. 2. Scroll to and select PROGRAMMED TESTS. 3. Scroll to and select ALL TESTS (or another sequence containing 51 Iron Bipyr) from TESTING MENU. 4. Scroll to and select 51 Iron Bipyr from menu. 5. Rinse a clean tube (0290) with sample water. Fill to the 10 mL line with sample. 6. Insert tube into chamber, close lid and select SCAN BLANK. 7. Remove the tube from Spectro. Use the 0.
IRON I,I0-PHENANTHROLINE METHOD • CODE 3668-SC QUANTITY CONTENTS CODE 15 mL *Acid Phenanthroline Indicator *2776-E 5g *Iron Reducing Reagent *2777-C Spoon, 0.1 g, plastic 0699 1 *WARNING: Reagents marked with an * are considered to be potential health hazards. To view or print a Material Safety Data Sheet (MSDS) for these reagents go to www.lamotte.com. To obtain a printed copy, contact LaMotte by e-mail, phone or fax.
PROCEDURE Test Procedures Use universal sample holder. 1. Press and hold ON button until spectrophotometer turns on. 2. Scroll to and select PROGRAMMED TESTS. 3. Scroll to and select ALL TESTS (or another sequence containing 53 Iron Phen) from TESTING MENU. 4. Scroll to and select 53 Iron Phen from menu. 5. Rinse a clean tube (0290)with sample water. Fill to the 10 mL mark with sample. 6. Insert tube into chamber, close lid and select SCAN BLANK. 7. Remove the tube from Spectro.
IRON, UDV BIPYRIDYL–UNIT DOSE VIAL METHOD • CODE 4315-J QUANTITY 1 CONTENTS *Total Iron Unit Dose Vials, 20 pouches CODE *4315-J *WARNING: Reagents marked with an * are considered to be potential health hazards. To view or print a Material Safety Data Sheet (MSDS) for these reagents go to www.lamotte.com. To obtain a printed copy, contact LaMotte by e-mail, phone or fax.
Drinking, surface, and saline waters; domestic and industrial wastes. RANGE: 0.00–10.00 ppm MDL: 0.07 ppm METHOD: Ferric iron is reduced to ferrous iron and subsequently forms a colored complex with for a quantitative measure of total iron. SAMPLE HANDLING & PRESERVATION: The sample container should be cleaned with acid and rinsed with deionized water. Addition of acid to adjust the sample. The pH 2-3 will prevent depositation of iron on the container walls.
PROCEDURE Use 10 mm square cell adapter. 1. Press and hold ON button. 2. Scroll to and select PROGRAMMED TESTS. 3. Scroll to and select ALL TESTS (or another sequence containing 52 Iron-UDV) from TESTING MENU. 4. Scroll to and select 52 Iron-UDV from menu. 5. Rinse a clean vial (0156) with sample water. 6. Use the syringe (1184) to add 3 mL of sample to the vial. 7. Insert the vial into the chamber, close the lid and select SCAN BLANK. 8. Remove the vial from the Spectro. 9.
Test Procedures IRON, UDV SMART Spectro Test Procedures 2.
LEAD PAR METHOD • CODE 4031 QUANTITY CONTENTS CODE 250 mL *Ammonium Chloride Buffer *4032-K 15 mL *Sodium Cyanide, 10% *6565-E 30 mL *PAR Indicator *4033-G 30 mL 15 mL Stabilizing Reagent *DDC Reagent 4022-G *4034-E 1 Syringe, 5 mL, plastic 0807 2 Pipet, 0.5 mL, plastic 0353 *WARNING: Reagents marked with an * are considered to be potential health hazards. To view or print a Material Safety Data Sheet (MSDS) for these reagents go to www.lamotte.com.
PROCEDURE Use universal sample holder. 1. Press and hold ON button until spectrophotometer turns on. 2. Scroll to and select PROGRAMMED TESTS. 3. Scroll to and select ALL TESTS (or another sequence containing 54 Lead) from TESTING MENU. 4. Scroll to and select 54 Lead from menu. 5. Rinse a tube (0290) with sample water. Fill to 10 mL with sample. 6. Insert the tube into chamber, close lid and select SCAN BLANK. 7. Remove the tube from Spectro. Empty the tube.
MANGANESE, LOW RANGE PAN METHOD • CODE 3658-01-SC QUANTITY CONTENTS CODE 2 x 60 mL *Hardness Buffer Reagent *4255-H 30 mL *Manganese Indicator Reagent *3956-G 15 mL *Sodium Cyanide, 10% *6565-E 1 Pipet, 0.5 mL, plastic 0369 1 Pipet, 1.0 mL, plastic 0354 *WARNING: Reagents marked with an * are considered to be potential health hazards. To view or print a Material Safety Data Sheet (MSDS) for these reagents go to www.lamotte.com.
PROCEDURE Use universal sample holder. 1. Press and hold ON button until spectrophotometer turns on. 2. Scroll to and select PROGRAMMED TESTS. 3. Scroll to and select ALL TESTS (or another sequence containing 55 Manganese L) from TESTING MENU. 4. Scroll to and select 55 Manganese L from menu. 5. Rinse a tube (0290) with sample water. Fill to the 10 mL line with sample. 6. Insert tube into chamber, close lid and select SCAN BLANK. 7. Remove tube from Spectro. Use the 1.
MANGANESE, HIGH RANGE PERIODATE METHOD • CODE 3669-SC QUANTITY CONTENTS CODE 10 g Manganese Buffer Reagent 6310-D 15 g *Manganese Periodate Reagent *6311-E 1 Spoon, 0.1 g, plastic 0699 1 Spoon, 0.15 g, plastic 0727 *WARNING: Reagents marked with an * are considered to be potential health hazards. To view or print a Material Safety Data Sheet (MSDS) for these reagents go to www.lamotte.com. To obtain a printed copy, contact LaMotte by e-mail, phone or fax.
PROCEDURE Test Procedures Use universal sample holder. 1. Press and hold ON button until spectrophotometer turns on. 2. Scroll to and select PROGRAMMED TESTS. 3. Scroll to and select ALL TESTS (or another sequence containing 56 Manganese H) from TESTING MENU. 4. Scroll to and select 56 Manganese H from menu. 5. Rinse a tube (0290) with sample water. Fill to the 10 mL line with sample. 6. Insert tube into chamber, close lid and select SCAN BLANK. 7. Remove tube from Spectro. Use the 0.
MERCURY TMK METHOD • CODE 4861 QUANTITY CONTENTS CODE 50 *TMK Tablets *4862-H 2 x 250 mL *Propyl Alcohol *4863-K 250 mL *Acetate Buffer *4864-K 1 Tablet Crusher 0175 1 Test Tube, 10 mL, glass, w/cap 0778 1 Pipet, 1.0 mL, plastic 0354 1 Pipet, 0.5 mL, plastic 0353 *WARNING: Reagents marked with an * are considered to be potential health hazards. To view or print a Material Safety Data Sheet (MSDS) for these reagents go to www.lamotte.com.
PREPARATION OF *TMK INDICATOR NOTE: Prepare *TMK Indicator daily. Keep out of direct sunlight. 1. Fill test tube (0778) to the 10 mL line with *Propyl Alcohol (4863). 2. Add one *TMK Tablet (4862). 3. Use tablet crusher (0175) to completely crush tablet. 4. Cap and mix. Shake vigorously for 30 seconds. PROCEDURE Test Procedures Use universal sample holder. 1. Press and hold ON button until spectrophotometer turns on. 2. Scroll and select PROGRAMMED TESTS. 3.
MOLYBDENUM THIOGLYCOLATE METHOD • CODE 3699-02-SC QUANTITY CONTENTS CODE 2 x 30 mL *Mo Buffer *3997-G 2 x 30 mL *Molybdenum Oxidizing Reagent *6485-G 2.5g *Molybdenum Indicator Powder *6486-S 1 Spoon, 0.05g, plastic 0696 2 Pipets, 1.0 mL, plastic w/cap 0372 *WARNING: Reagents marked with an * are considered to be potential health hazards. To view or print a Material Safety Data Sheet (MSDS) for these reagents go to www.lamotte.com.
PROCEDURE Use universal sample holder. 1. Press and hold ON button until spectrophotometer turns on. 2. Scroll to and select PROGRAMMED TESTS. 3. Scroll to and select ALL TESTS (or another sequence containing 61 Molybdenum-HR) from TESTING MENU. 4. Scroll to and select 61 MOLYBDENUM-HR from menu. 5. Fill clean tube (0290) to 10 mL line with sample water. 6. Insert tube into chamber, close lid and select SCAN BLANK. 7. Remove tube from Spectro. Use a 1.0 mL pipet (0372) to add 1.
NICKEL DIMETHYLGLYOXIME METHOD • CODE 3663-SC QUANTITY CONTENTS CODE 60 mL *Hydrochloric Acid, 2.5N *6251PS-H 30 g *Ammonium Persulfate Reagent *6566-G 30 mL *Silver Nitrate Solution, 0.0141N *6346WT-G 250 mL Sodium Citrate, 10% 6253-K 60 mL *Dimethylglyoxime, 1% *6254-H 60 mL *Ammonium Hydroxide, Conc. *6537-H 3 Pipets, 1.0 mL, plastic 0354 1 Spoon, 0.1 g, plastic 0699 1 Test tube, 5-10-12.
PROCEDURE Test Procedures Use universal sample holder. 1. Use the 10 mL graduated cylinder (0416) to measure 10 mL of sample water. Pour into glass test tube (0608). 2. Use the 1 mL pipet (0354) to add 1 mL of *Hydrochloric Acid, 2.5N (6251). 3. Use the 0.1 g spoon (0699) to add 2 measures of *Ammonium Persulfate Reagent (6566). Add two drops of *Silver Nitrate Solution, 0.0141N (6346WT). Mix until the powder has dissolved. The solution will be slightly cloudy at this point. 4.
NITRATE ZINC REDUCTION TESTAB METHOD • CODE 3689-SC QUANTITY 50 CONTENTS *Nitrate Spectrophotometic Grade Tablets 1 Tablet Crusher CODE *3881A-H 0175 *WARNING: Reagents marked with an * are considered to be potential health hazards. To view or print a Material Safety Data Sheet (MSDS) for these reagents go to www.lamotte.com. To obtain a printed copy, contact LaMotte by e-mail, phone or fax.
PROCEDURE Use universal sample holder 1. Press and hold ON button until spectrophotometer turns on. 2. Scroll to and select PROGRAMMED TESTS. 3. Scroll to and select ALL TESTS (or another sequence containing 66 Nitrate-TT) from TESTING MENU. 4. Scroll to and select 66 Nitrate-TT from menu. 5. Rinse a tube (0290) with sample water. Fill to 10 mL with sample. 6. Insert the tube into chamber, close lid and select SCAN BLANK. 7. Remove the tube from Spectro. 8.
NITRATE-NITROGEN, LOW RANGE CADMIUM REDUCTION METHOD • CODE 3649-SC QUANTITY CONTENTS CODE 2 x 60 mL *Mixed Acid Reagent *V-6278-H 5g *Nitrate Reducing Reagent *V-6279-C 1 Spoon, 0.1 g, plastic 0699 1 Dispenser Cap 0692 *WARNING: Reagents marked with an * are considered to be potential health hazards. To view or print a Material Safety Data Sheet (MSDS) for these reagents go to www.lamotte.com. To obtain a printed copy, contact LaMotte by e-mail, phone or fax. U.S.
Drinking, surface, and saline waters; domestic and industrial waters. RANGE: 0.00–3.00 ppm Nitrate Nitrogen MDL: 0.02 ppm METHOD: Powdered cadmium is used to reduce nitrate to nitrite. The nitrite that is originally present plus reduced nitrate is determined by diazotization of sulfanilamide and nitrite followed by coupling with N-(1 naphthyl)ethylenediamine dihydrochloride to form a highly colored azo dye which is measured colorimetrically.
PROCEDURE Use universal sample holder. NOTE: Place Dispenser Cap (0692) on *Mixed Acid Reagent (V-6278). Save this cap for refill reagents. Press and hold ON button until spectrophotometer turns on. 2. Scroll to and select PROGRAMMED TESTS. 3. Scroll to and select ALL TESTS (or another sequence containing 64 Nitrate-N LR) from TESTING MENU. 4. Scroll to and select 64 Nitrate-N LR from menu. 5. Rinse a clean tube (0290) with sample water. Fill to 10 mL line with sample. 6.
Test Procedures NITRATE-NITROGEN, Low Range SMART Spectro Test Procedures 2.
NITRITE ZINC REDUCTION TESTAB METHOD • CODE 3694-SC QUANTITY 50 CONTENTS *Nitrite Spectrophotometric Grade Tablets 1 Tablet Crusher CODE *3886A-H 0175 *WARNING: Reagents marked with an * are considered to be potential health hazards. To view or print a Material Safety Data Sheet (MSDS) for these reagents go to www.lamotte.com. To obtain a printed copy, contact LaMotte by e-mail, phone or fax.
PROCEDURE Use universal sample holder 1. Press and hold ON button until spectrophotometer turns on. 2. Scroll to and select PROGRAMMED TESTS. 3. Scroll to and select ALL TESTS (or another sequence containing 69 Nitrite-TT) from TESTING MENU. 4. Scroll to and select 69 Nitrite-TT from menu . 5. Rinse a tube (0290) with sample water. Fill to 10 mL with sample. 6. Insert the tube into chamber, close lid and select SCAN BLANK. 7. Remove the tube from Spectro. 8.
NITRITE-NITROGEN, LOW RANGE DIAZOTIZATION METHOD • CODE 3650-SC QUANTITY CONTENTS CODE 2 x 60 mL *Mixed Acid Reagent *V-6278-H 5g *Color Developing Reagent *V-6281-C 1 Spoon, 0.1 g, plastic 0699 1 Dispenser Cap 0692 *WARNING: Reagents marked with an * are considered to be potential health hazards. To view or print a Material Safety Data Sheet (MSDS) for these reagents go to www.lamotte.com. To obtain a printed copy, contact LaMotte by e-mail, phone or fax.
PROCEDURE Use universal sample holder. Test Procedures NOTE: Place Dispenser Cap (0692) on *Mixed Acid Reagent (V-6278). Save this cap for refill reagents. 1. Press and hold ON button until spectrophotometer turns on. 2. Scroll to and select PROGRAMMED TESTS. 3. Scroll to and select ALL TESTS (or another sequence containing 67 Nitrite-N LR) from TESTING MENU. 4. Scroll to and select 67 Nitrite-N LR from menu. 5. Rinse a clean tube (0290) with sample water. Fill to the 10 mL line with sample. 6.
NITROGEN, TOTAL CHROMOTROPIC ACID WITH PERSULFATE DIGESTION METHOD • CODE 4026 QUANTITY CONTENTS CODE 25 Total Nitrogen Hydroxide Reagent Tubes 4040-G 5g *Digestion Reagent Powder 60 mL Deionized Water 4036-C *5115PS-H 5g *Total Nitrogen Reagent A Powder *4041-C 30 *Total Nitrogen Reagent B Tablets *4042A-G 25 *Total Nitrogen Acid Reagent Tubes *4043-G 2 Spoon, 0.15 g, plastic 0727 4 Pipets, 1.
Nitrogen is essential for plant growth, but the presence of excessive amounts in water supplies presents a major pollution problem. Nitrogen compounds may enter water as nitrates or be converted to nitrates from agricultural fertilizers, sewage, industrial and packing house wastes, drainage from livestock feeding areas, farm manures and legumes. Nitrates in large amounts can cause “blue babies” (methemoglobinemia) in infants less than six months of age.
PROCEDURE Use universal sample holder 1. Preheat COD reactor to 100 ±2°C. Follow safety precautions. 2. Remove caps from two *Total Nitrogen Hydroxide Reagent Tubes (4040). 3. Use a 0.15 g spoon (0727) and a funnel (0459) to add one level measure of *Digestion Reagent Powder (4036) to each tube. Tap funnel to dispense powder completely. 4. Use a 1.0 mL pipet (0354) to add 2.0 mL of Deionized Water (5115PS), or organic-free water, to one tube. This is the blank. 5. Use another 1.
NOTE: Invert slowly and completely for accurate results. Hold tubes with caps up. Invert the tube and wait for the air bubble to flow to the bottom of the tube. Turn the tube upright and wait for the air bubble to return to the top of the tube. This is one inversion. 22. Wait 5 minutes. 23. Wipe the tubes with a damp towel to remove fingerprints and smudges. Wipe with a dry towel. 24. Insert the blank tube into the chamber. Select SCAN BLANK. Remove the blank tube from the spectrophotometer. 25.
OXYGEN SCAVENGERS TEST FOR DEHA (DIETHYLHYDROXYLAMINE), CARBOHYDRAZIDE, ERYTHORBIC ACID, HYDROQUINONE, METHYLETHYLKETOXIME IRON REDUCTION METHOD • CODE 4857-01 QUANTITY CONTENTS CODE 15 mL *DEHA Reagent #1 *4791-E 15 mL *DEHA Reagent #2 *4792-E 15 mL *DEHA Reagent #3 *4793-E *WARNING: Reagents marked with an * are considered to be potential health hazards. To view or print a Material Safety Data Sheet (MSDS) for these reagents go to www.lamotte.com.
DEHA PROCEDURE Use universal sample holder 1. Press and hold ON button until spectrophotometer turns on. 2. Scroll to and select PROGRAMMED TESTS. 3. Scroll to and select ALL TESTS (or another sequence containing 38 DEHA) from TESTING MENU. 4. Scroll to and select 38 DEHA from menu. 5. Rinse a tube (0290) with sample water. Fill to 10 mL with sample. 6. Insert the tube into chamber, close lid and select SCAN BLANK. 7. Remove the tube from spectrophotometer. 8.
CARBOHYDRAZIDE PROCEDURE Use universal sample holder 1. Press and hold ON button until spectrophotometer turns on. 2. Scroll to and select PROGRAMMED TESTS. 3. Scroll to and select ALL TESTS (or another sequence containing 14 c-hydrazide) from TESTING MENU. 4. Scroll to and select 14 c-hydrazide from menu. 5. Rinse a tube (0290) with sample water. Fill to 10 mL with sample. 6. Insert the tube into chamber, close lid and select SCAN BLANK. 7. Remove the tube from spectrophotometer. 8.
ERYTHORBIC ACID PROCEDURE Use universal sample holder 1. Press and hold ON button until spectrophotometer turns on. 2. Scroll to and select PROGRAMMED TESTS. 3. Scroll to and select ALL TESTS (or another sequence containing 40 E-thorbic A) from TESTING MENU. 4. Scroll to and select 40 E-thorbic A from menu. 5. Rinse a tube (0290) with sample water. Fill to 10 mL with sample. 6. Insert the tube into chamber, close lid and select SCAN BLANK. 7. Remove the tube from spectrophotometer. 8.
HYDROQUINONE PROCEDURE Use universal sample holder 1. Press and hold ON button until spectrophotometer turns on. 2. Scroll to and select PROGRAMMED TESTS. 3. Scroll to and select ALL TESTS (or another sequence containing 49 H-quinone) from TESTING MENU. 4. Scroll to and select 49 H-quinone from menu. 5. Rinse a tube (0290) with sample water. Fill to 10 mL with sample. 6. Insert the tube into chamber, close lid and select SCAN BLANK. 7. Remove the tube from spectrophotometer. 8.
METHYLETHYLKETOXIME PROCEDURE Use universal sample holder 1. Press and hold ON button until spectrophotometer turns on. 2. Scroll to and select PROGRAMMED TESTS. 3. Scroll to and select ALL TESTS (or another sequence containing 58 m-e-ketoxim) from TESTING MENU. 4. Scroll to and select 58 m-e-ketoxim from menu. 5. Rinse a tube (0290) with sample water. Fill to 10 mL with sample. 6. Insert the tube into chamber, close lid and select SCAN BLANK. 7. Remove the tube from spectrophotometer. 8.
OZONE INDIGO METHOD • CODE 365I-SC QUANTITY CONTENTS CODE 15 mL Chlorine Inhibitor 3990-E 250 mL *Ozone Buffer *3991-K 30 mL Indigo Blue Stock Solution 3989-G 1 Sampling Apparatus 0681 1 Pipet, transfer, 1.0 mL 2-2170 1 Pipet, transfer, 5 mL 0329 1 Pump, 10 mL 30527 1 Bottle, HR Reagent, amber glass 0680-J 1 Graduated Cylinder, 50 mL, glass 0418 *WARNING: Reagents marked with an * are considered to be potential health hazards.
PROCEDURE–LOW RANGE Use universal sample holder. A. PREPARATION OF HR REAGENT NOTE: The quantity of Indigo Blue Stock solution (3989) supplied will prepare one batch of HR Reagent for the High Range Ozone procedure or five batches of HR Reagent for the Low Range Ozone procedure. 1. Use the 50 mL graduated cylinder to carefully add 45 mL of *Ozone Buffer (3991) to amber glass bottle marked HR Reagent (0680). 2.
NOTE: HR Reagent must be made fresh each week. If reagent is refrigerated, it may be kept up to 3 weeks. PROCEDURE–HIGH RANGE Use universal sample holder. A. PREPARATION OF HR REAGENT NOTE: The quantity of Indigo Blue Stock solution (3989) supplied will prepare one batch of HR Reagent for the High Range Ozone procedure or five batches of HR Reagent for the Low Range Ozone procedure. 1.
14. Insert reacted Sample Tube into chamber, close lid and select SCAN SAMPLE. Record result. 15. Press OFF button to turn spectrophotometer off or press EXIT button to exit to a previous menu or make another menu selection. Test Procedures NOTE: HR Reagent must be made fresh each week. If reagent is refrigerated, it may be kept up to 3 weeks. OZONE SMART Spectro Test Procedures 2.
pH COLORIMETRIC METHOD • CODE 3700-01-SC QUANTITY CONTENTS CODE 60 mL Chlorphenol Red Indicator V-2209-H 60 mL Phenol Red Indicator V-2304-H 60 mL Thymol Blue Indicator V-2213-H 3 Pipets, 0.5 mL, plastic w/caps 0369 The term pH (always written with a lower case p and an upper case H) is correctly defined as the negative logarithm of the hydrogen ion concentration.
APPLICATION: Drinking, surface, and saline waters; swimming pool water; domestic and industrial wastes. RANGE: The various pH indicators exhibit a specific color change over a narrow pH range. The color changes are measured colorimetrically. METHOD: Sample should be analyzed immediately after collection. SAMPLE HANDLING & PRESERVATION: Sample color and turbidity interfere with the colorimetric pH measurement.
PROCEDURE Use universal sample holder. 1. Use Indicator, Range, & Test Name chart to select the indicator, corresponding to anticipated pH range and to determine corresponding test name to select from spectrophotometer menu. 2. Press and hold ON button until spectrophotometer turns on. 3. Scroll to and select PROGRAMMED TESTS. 4. Scroll to and select ALL TESTS (or another sequence containing the appropriate pH test name) from TESTING MENU. 5.
Test Procedures pH SMART Spectro Test Procedures 2.
PHENOL AMINOANTIPYRINE METHOD • CODE 3652-SC QUANTITY CONTENTS CODE 5g Aminoantipyrine Reagent 7825-C 30 mL *Ammonium Hydroxide Solution *7826-G 2 x 60 mL *Potassium Ferricyanide Solution *7827-H 1 Spoon, 0.1 g, plastic 0699 1 Pipet, plain, plastic 0352 1 Pipet, 1.0 mL, plastic 0354 *WARNING: Reagents marked with an * are considered to be potential health hazards. To view or print a Material Safety Data Sheet (MSDS) for these reagents go to www.lamotte.com.
PROCEDURE Use universal sample holder. 1. Press and hold ON button until spectrophotometer turns on. 2. Scroll to and select PROGRAMMED TESTS. 3. Scroll to and select ALL TESTS (or another sequence containing 77 Phenol) from TESTING MENU. 4. Scroll to and select 77 Phenol from menu. 5. Rinse a clean tube (0290) with sample water. Fill to the 10 line with sample. 6. Insert tube into chamber, close lid and select SCAN BLANK. 7. Remove tube from Spectro. Use the 0.
PHOSPHATE–LOW RANGE ASCORBIC ACID REDUCTION METHOD CODE 3653-SC QUANTITY CONTENTS CODE 60 mL *Phosphate Acid Reagent *V-6282-H 5g *Phosphate Reducing Reagent *V-6283-C 1 Pipet, 1 mL, plastic 0354 1 Spoon, 0.1 g, plastic 0699 *WARNING: Reagents marked with an * are considered to be potential health hazards. To view or print a Material Safety Data Sheet (MSDS) for these reagents go to www.lamotte.com. To obtain a printed copy, contact LaMotte by e-mail, phone or fax.
Test Procedures APPLICATION: Drinking, surface, and saline waters; domestic and industrial wastes (Method based on reactions that are specific for orthophosphate). RANGE: 0.00–3.00 ppm Orthophosphate MDL: 0.04 ppm METHOD: Ammonium molybdate and antimony potassium tartrate react in a filtered acid medium with dilute solution of PO4–3 to form an antimony-phosphomolybdate complex. This complex is reduced to an intense blue colored complex by ascorbic acid.
PROCEDURE Use universal sample holder. 1. Press and hold ON button until spectrophotometer turns on. 2. Scroll to and select PROGRAMMED TESTS. 3. Scroll to and select ALL TESTS (or another sequence containing 78 Phosphate-L) from TESTING MENU. 4. Scroll to and select 78 Phosphate-L from menu. 5. Rinse a clean tube (0290) with sample water. Fill to the 10 line with sample. 6. Insert tube into chamber, close lid and select SCAN BLANK. 7. Remove tube from Spectro. Use 1.0 mL pipet (0354) to add 1.
Test Procedures PHOSPHATE, Low Range SMART Spectro Test Procedures 2.
PHOSPHATE–HIGH RANGE VANADOMOLYBDOPHOSPHORIC ACID METHOD CODE 3655-SC QUANTITY 4 x 30 mL CONTENTS CODE *VM Phosphate Reagent *4410-G Pipet, 1.0 mL, plastic 0354 1 *WARNING: Reagents marked with an * are considered to be potential health hazards. To view or print a Material Safety Data Sheet (MSDS) for these reagents go to www.lamotte.com. To obtain a printed copy, contact LaMotte by e-mail, phone or fax.
PROCEDURE Use universal sample holder. Press and hold ON button until spectrophotometer turns on. 2. Scroll to and select PROGRAMMED TESTS. 3. Scroll to and select ALL TESTS (or another sequence containing 79 Phosphate-H) from TESTING MENU. 4. Scroll to and select 79 Phosphate-H from menu. 5. Rinse a clean tube (0290) with sample water. Fill to the 10 mL line with sample. 6. Insert tube into chamber, close lid and select SCAN BLANK. 7. Remove tube from Spectro. Use the 1.
PHOSPHORUS-TOTAL, LOW RANGE ASCORBIC ACID REDUCTION WITH PERSULFATE DIGESTION METHOD • CODE 4024-01 QUANTITY CONTENTS CODE 25 *Total Phosphorus Acid Reagent Tubes *4035-G 5g *Digestion Reagent Powder *4036-C 2 X 30 mL *Total Phosphorus LR Hydroxide Reagent *4038-G 2 X 30 mL *Phosphate Acid Reagent *V-6282-G 5g Phosphate Reducing Reagent V-6283-C 1 Spoon, 0.15 g, plastic 0727 3 Pipets, 1.0 mL, plastic 0354 1 Spoon, 0.
Test Procedures Phosphorus in natural waters and wastewaters occurs almost exclusively in the form of orthophosphates, condensed phosphates (pyro-, meta- and other polyphosphates) and organically bound phosphates. Phosphates may be added in small amounts to water supplies during treatment. Larger amounts are introduced to water used for cleaning or laundering as components of commercial cleaning preparations.
PROCEDURE Use universal sample holder 1. Preheat COD reactor to 150 ±2°C. Follow safety precautions. 2. Remove cap from a *Total Phosphorus Acid Reagent Tube (4035). Use a 1.0 mL pipet (0354) to add 5.0 mL of sample. 3. Use the 0.15 g spoon (0727) and a funnel (0459) to add one level measure of *Digestion Reagent Powder (4036). Tap funnel to dispense powder completely. Cap tube tightly and shake until powder dissolves completely. 4. Place the tube in the COD reactor for 30 minutes. 5.
Test Procedures PHOSPHORUS-TOTAL, Low Range SMART Spectro Test Procedures 2.
PHOSPHORUS-TOTAL, HIGH RANGE MOLYBDOVANADATE WITH ACID PERSULFATE DIGESTION METHOD • CODE 4025-01 QUANTITY 25 CONTENTS CODE *Total Phosphorus Acid Reagent Tubes 60 mL Deionized Water *4035-G 5115PS-H 5g *Digestion Reagent Powder *4036- C 2 X 30 mL *Total Phosphorus HR Hydroxide Reagent *4037-G 30 mL *Total Phosphorus HR Indicator Reagent *4039-G 1 Spoon, 0.15 g 0727 3 Pipets 1.0 mL, plastic 0354 1 Pipet, 0.
Test Procedures Phosphorus in natural waters and wastewaters occurs almost exclusively in the form of orthophosphates, condensed phosphates (pyro-, meta- and other polyphosphates) and organically bound phosphates. Phosphates may be added in small amounts to water supplies during treatment. Larger amounts are introduced to water used for cleaning or laundering as components of commercial cleaning preparations.
PROCEDURE Use universal sample holder Preheat COD reactor to 150 ±2°C. Follow safety precautions. 2. Remove cap from a *Total Phosphorus Acid Reagent Tube (4035). Use a 1.0 mL pipet (0354) to add 5.0 mL of Deionized Water (5115PS). This is the blank. 3. Remove cap from a *Total Phosphorus Acid Reagent Tube (4035). Use another 1.0 mL pipet (0354) to add 5.0 mL of sample water. This is the sample. 4. Use the 0.
Test Procedures PHOSPHORUS-TOTAL, High Range SMART Spectro Test Procedures 2.
POTASSIUM TETRAPHENYLBORON METHOD • CODE 3639-SC QUANTITY CONTENTS CODE 30 mL *Sodium Hydroxide, 1.0N *4004WT-G 5g *Tetraphenylboron Powder *6364-C 1 Spoon, 0.05 g, plastic 0696 *WARNING: Reagents marked with an * are considered to be potential health hazards. To view or print a Material Safety Data Sheet (MSDS) for these reagents go to www.lamotte.com. To obtain a printed copy, contact LaMotte by e-mail, phone or fax.
PROCEDURE Test Procedures Use universal sample holder. 1. Press and hold ON button until spectrophotometer turns on. 2. Scroll to and select PROGRAMMED TESTS. 3. Scroll to and select ALL TESTS (or another sequence containing 81 Potassium) from TESTING MENU. 4. Scroll to and select 81 Potassium from menu. 5. Rinse a clean tube (0290) with sample water. Fill to the 10 mL line with sample. 6. Insert tube into chamber, close lid and select SCAN BLANK. 7. Remove tube from Spectro.
SILICA–LOW RANGE HETEROPOLY BLUE METHOD • CODE 3664-SC QUANTITY CONTENTS CODE 30 mL *Silica Reagent #1 *V-4466-G 30 mL *Silica Reagent #2 *V-4467-G 30 mL *Silica Reagent #3 *V-4468-G 10 g *Silica Reagent #4 *V-6284-D 1 Spoon, 0.1 g, plastic 0699 *WARNING: Reagents marked with an * are considered to be potential health hazards. To view or print a Material Safety Data Sheet (MSDS) for these reagents go to www.lamotte.com. To obtain a printed copy, contact LaMotte by e-mail, phone or fax.
Drinking, surface, and saline waters; domestic and industrial wastes. RANGE: 0.00–2.50 ppm Silica MDL: 0.03 ppm METHOD: Reactive silica forms a complex with ammonium molybdate in an acidic solution to produce a yellowgreen color in proportion to the amount of silica present. Phosphate also reacts with molybdate but the addition of oxalic acid eliminates the molybdophosphoric acid complex. This silica molybdate complex is then reduced by ascorbic acid to produce an intense blue color.
PROCEDURE Use universal sample holder. 1. Press and hold ON button until spectrophotometer turns on. 2. Scroll to and select PROGRAMMED TESTS. 3. Scroll to and select ALL TESTS (or another sequence containing 85 Silica Lo) from TESTING MENU. 4. Scroll to and select 85 Silica Lo from menu. 5. Rinse a clean tube (0290) with sample water. Fill to the 10 mL line with sample. 6. Insert tube into chamber, close lid and select SCAN BLANK. (See Note) 7. Remove tube from Spectro.
Test Procedures SILICA, Low Range SMART Spectro Test Procedures 2.
SILICA–HIGH RANGE SILICOMOLYBDATE METHOD • CODE 3687-SC QUANTITY CONTENTS CODE 30 mL *Silica Reagent #1 *V-4466-G 30 mL *Silica Reagent #2 *V-4467-G 15 mL *Silica Reagent #3 *V-4468-G *WARNING: Reagents marked with an * are considered to be potential health hazards. To view or print a Material Safety Data Sheet (MSDS) for these reagents go to www.lamotte.com. To obtain a printed copy, contact LaMotte by e-mail, phone or fax.
Boiler and cooling waters; domestic and industrial wastes. RANGE: 0–50 ppm Silica MDL: 1 ppm METHOD: Silica forms a complex with ammonium molybdate in an acidic solution to produce a yellow color in proportion to the amount of silica present. Phosphate also reacts with molybdate but the addition of oxalic acid eliminates the molybdophosphoric acid complex.
PROCEDURE Use universal sample holder. 1. Press and hold ON button until spectrophotometer turns on. 2. Scroll to and select PROGRAMMED TESTS. 3. Scroll to and select ALL TESTS (or another sequence containing 86 Silica Hi) from TESTING MENU. 4. Scroll to and select 86 Silica Hi from menu. 5. Rinse a clean tube (0290) with sample water. Fill to the 10 mL line with sample. 6. Insert tube into chamber, close lid and select SCAN BLANK. 7. Remove tube from Spectro.
Test Procedures SILICA, High Range SMART Spectro Test Procedures 2.
SULFATE BARIUM CHLORIDE METHOD • CODE 3665-SC QUANTITY 10 g CONTENTS *Sulfate Reagent 1 Spoon, 0.1 g, plastic CODE *V-6277-D 0699 *WARNING: Reagents marked with an * are considered to be potential health hazards. To view or print a Material Safety Data Sheet (MSDS) for these reagents go to www.lamotte.com. To obtain a printed copy, contact LaMotte by e-mail, phone or fax. APPLICATION: Drinking and surface waters; domestic and industrial wastes.
PROCEDURE Test Procedures Use universal sample holder. 1. Press and hold ON button until spectrophotometer turns on. 2. Scroll to and select PROGRAMMED TESTS. 3. Scroll to and select ALL TESTS (or another sequence containing 89 SULFATE-HR) from TESTING MENU. 4. Scroll to and select 89 SULFATE-HR from menu. 5. Rinse a clean tube (0290) with sample water. Fill to the 10 mL line with sample. 6. Insert tube into chamber, close lid and select SCAN BLANK. 7. Remove tube from Spectro. Use the 0.
SULFIDE METHYLENE BLUE METHOD • CODE 3654-02-SC QUANTITY CONTENTS CODE 2 X 30 mL *Sulfide Reagent A *V-4458-G 15 mL *Sulfide Reagent B *V-4459-E 2 x 60 mL Sulfide Reagent C 4460-H 2 Pipets, 1.0 mL, plastic 0354 *WARNING: Reagents marked with an * are considered to be potential health hazards. To view or print a Material Safety Data Sheet (MSDS) for these reagents go to www.lamotte.com. To obtain a printed copy, contact LaMotte by e-mail, phone or fax. SMART Spectro Test Procedures 2.
Drinking, surface, and saline waters; domestic and industrial wastes. RANGE: 0.00–1.00 ppm Sulfide MDL: 0.02 ppm METHOD: Under suitable conditions the sulfide ion reacts with p-aminodimethylaniline and ferric chloride to produce methylene blue in proportion to the sulfide concentration. Ammonium phosphate is added to remove the color due to the ferric iron.
PROCEDURE Use universal sample holder. 1. Press and hold ON button until spectrophotometer turns on. 2. Scroll to and select PROGRAMMED TESTS. 3. Scroll to and select ALL TESTS (or another sequence containing 90 Sulfide-LR) from TESTING MENU. 4. Scroll to and select 90 Sulfide-LR from menu. 5. Rinse a clean tube (0290) with sample water. Fill to the 10 mL line with sample. 6. Insert tube into chamber, close lid and select SCAN BLANK. 7. Remove tube from Spectro. Use the 1.
Test Procedures SULFIDE SMART Spectro Test Procedures 2.
SURFACTANTS ION PAIR EXTRACTION–BROMPHENOL BLUE INDICATOR METHOD • CODE 4876-01 QUANTITY CONTENTS CODE 50 g pH Adjustment Powder 4509- H 10 g Sodium Chloride Reagent 4877-D 2 X 60 mL *DS Indicator Reagent *4508-H 1 Spoon, 0.5 g, plastic 0698 1 Spoon, 0.1 g, plastic 0699 1 Pipet, 1.0 mL, plastic 0354 *WARNING: Reagents marked with an * are considered to be potential health hazards. To view or print a Material Safety Data Sheet (MSDS) for these reagents go to www.lamotte.com.
PROCEDURE Use universal sample holder. 1. Press and hold ON button until spectrophotometer turns on. 2. Scroll to and select PROGRAMMED TESTS. 3. Scroll to and select ALL TESTS (or another sequence containing 94 Surfactants) from TESTING MENU. 4. Scroll to and select 94 Surfactants from menu. 5. Rinse a tube (0290) with sample water. Fill to 10 mL line with sample. 6. Insert the tube into chamber, close lid and select SCAN BLANK. 7. Remove the tube from Spectro. 8. Use the 0.
TANNIN TUNGSTO-MOLYBDOPHOSPHORIC ACID METHOD CODE 3666-01-SC QUANTITY CONTENTS CODE 30 mL *Tannin Reagent #1 *7833-G 2 x 60 mL *Tannin Reagent #2 *7834-H Pipet, plain, plastic 0352 Pipet, 1.0 mL, plastic 0354 *WARNING: Reagents marked with an * are considered to be potential health hazards. To view or print a Material Safety Data Sheet (MSDS) for these reagents go to www.lamotte.com. To obtain a printed copy, contact LaMotte by e-mail, phone or fax.
PROCEDURE Use universal sample holder. 1. Press and hold ON button until spectrophotometer turns on. 2. Scroll to and select PROGRAMMED TESTS. 3. Scroll to and select ALL TESTS (or another sequence containing 96 TANNIN) from TESTING MENU. 4. Scroll to and select 96 TANNIN from menu. 5. Rinse a clean tube (0290) with sample water. Fill to the 10 mL line with sample. 6. Insert tube into chamber, close lid and select SCAN BLANK. 7. Remove tube from Spectro.
TURBIDITY ABSORPTION METHOD • NO REAGENTS REQUIRED Turbidity is a measure of water clarity and is independent of color. Turbidity is caused by undissolved and suspended solids. Mud, silt, algae, and microorganisms can all cause turbidity. Turbidity is a gross measurement of water quality. APPLICATION: Surface and industrial waters for non-compliance monitoring. (For compliance monitoring at low turbidity levels, use a commercial .
FORMAZIN STOCK SOLUTION The turbidity calibration curve for this instrument was prepared by using formazin solutions as a reference. A 4000 FTU standard solution is available (Order Code 6195-H, 60 mL) that can be diluted with low turbidity water to prepare solutions within the test range.Dilutions from this stock solution should be prepared fresh daily with low turbidity water.
ZINC ZINCON METHOD • CODE 3667-SC QUANTITY CONTENTS CODE 30 mL *Zinc Indicator Solution *6314-G 120 mL *Methyl Alcohol *6319-J 10 g Sodium Ascorbate Powder 6316-D 25 g *Zinc Buffer Powder *6315-G 15 mL *Sodium Cyanide, 10% *6565-E 30 mL *Formaldehyde Solution, 37% *5128-G 1 “Dilute Zinc Indicator Solution” Bottle, with 1 mL pipet assembly 0128-MT 1 Graduated Cylinder, 10 mL, glass 0416 1 Spoon, 0.5 g, plastic 0698 2 Pipets, plain, plastic 0352 1 Spoon, 0.
APPLICATION: Drinking and surface waters; domestic and industrial wastewaters. RANGE: 0.00–3.00 ppm Zinc MDL: 0.025 ppm METHOD: Zinc forms a blue colored complex with Zincon in a solution buffered at pH 9.0. Other heavy metals are complexed by cyanide and the zinc cyanide complex is released by the addition of formaldehyde before the other metal cyanide complexes are destroyed. Sodium ascorbate is added to reduce the interference of manganese.
PROCEDURE Use universal sample holder. A. PREPARATION OF DILUTE ZINC INDICATOR SOLUTION 1. Use a pipet (0352) to measure exactly 5.0 mL of *Zinc Indicator Solution (6314) into 10 mL graduated cylinder (0416). The bottom of the curved surface (the meniscus) of liquid should be at 5.0 mL mark. Pour this into the bottle labeled “Dilute Zinc Indicator Solution”. 2. Use unrinsed graduated cylinder to add 10.0 mL and then 7.8 mL (total of 17.
Test Procedures determine the reagent blank only when a new lot number of reagents is obtained. ZINC SMART Spectro Test Procedures 2.
APPENDIX Ammonia in water occurs in two forms: toxic unionized ammonia (NH3) and the relatively non-toxic ionized form, ammonium ion (NH4+). This test method measures both forms as ammonia-nitrogen (NH3–N) to give the total ammonia-nitrogen concentration in water. The actual proportion of each compound depends on temperature, salinity, and pH. A greater concentration of unionized ammonia is present when the pH value and salinity increase. 1.
FOR EXAMPLE: If a fresh water sample at 20°C has a pH of 8.5 and the test result is 1.0 ppm as Total Ammonia-Nitrogen: 1. The percentage from the table is 11.18% (or 0.1118). 2. 1 ppm Total Ammonia-Nitrogen x 0.1118 = 0.1118 ppm Unionized AmmoniaNitrogen. 3. Total Ammonia-Nitrogen 1.0000 ppm _ 0.1118 ppm Ionized Ammonia-Nitrogen = 0.8882 ppm Appendix Unionized Ammonia-Nitrogen APPENDIX SMART Spectro Test Procedures 2.
